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Jurnal Fitopatologi Indonesia

jfiti
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Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

Arjuna Subject : -

Articles 361 Documents

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Identifikasi Tomato infectious chlorosis virus dan Tomato chlorosis virus melalui Reverse Transcription Polymerase Chain Reaction dan Analisis Sikuen Nukleotida Sari Nurulita; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Tomato chlorosis disease was found more frequent on production area in West Java recently. Observation in the field showed typical symptoms of Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV). The objective of this research is to identify TICV and ToCV associated with chlorosis disease on tomato plants in West Java (Cipanas, Lembang and Garut) using reverse transcription polymerase chain reaction (RT-PCR) method and nucleotide sequence analysis. DNA target of 417 bp and 360 bp was successfully amplified only from Cipanas sample using specific primers, TICV-CF/TICV-CR and ToCV-CF/ToCV-CR, respectively. Sequence analysis confirmed that chlorosis disease in Cipanas was associated with TICV and ToCV infection. Nucleotide sequence and phylogenetic analysis showed that TICV from Cipanas has high homology to and belongs to the same group with TICV from Japan and Spain; whereas ToCV from Cipanas has high homology to and belongs to the same group with ToCV from America.

Keefektifan Perlakuan Panas Kering dan Iradiasi UV-C untuk Mematikan Cendawan Model Microcyclus ulei Aprida Cristin; Meity Suradji Sinaga; Abdul Muin Adnan
Jurnal Fitopatologi Indonesia Vol 9 No 2 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

This study was carried out to develop treatment methods and determine the effective dose of dry heat treatment and UV-C irradiation to kill the fungus models of Microcyclus ulei on soybean grains and soybean meals without damaging its nutritional content. The fungus models used in this study were Colletotrichum gloeosporioides, Botryodiplodia theobromae, Fusarium oxysporum f. sp. niveum, and Sclerotium rolfsii. The results showed that UV-C treatment up to 12 and 24 hours exposure time at15 and 30 cm from UV-C light was not effective to kill all fungus models in PDA. Dry heat treatment at 55 °C for 30 and 60 minutes was effective to kill cultures of C. gloeosporioides, B. theobromae, and S. rolfsii in PDA, except for F. oxysporum f. sp. niveum. Further experiments on soybean showed that dry heat treatment at 55 °C for 30 minutes was effective to kill conidia of C. gloeosporioides, B. theobromae, and F. oxysporum f. sp. niveum, without damaging its protein content. However, dry heat treatment at 60 °C for 60 minutes has been proved not effective to kill sclerotia of S. rolfsii. If M. ulei had the similar resistance as S. rolfsii, then it would need a higher temperature and time than used in this study to kill the fungusKey words: Botryodiplodia theobromae, Colletotrichum gloeosporioides, effective doses, Fusarium oxysporum f. sp. niveum, Sclerotium rolfsii, soybean meals

Ketahanan Tiga Varietas Jahe terhadap Fusarium oxysporum f. sp. zingiberi in Vitro dan in Planta Dyah Pancasiwi; Soedarmono Soedarmono; Endang Mugiastuti; Loekas Soesanto
Jurnal Fitopatologi Indonesia Vol 9 No 2 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Fusarium oxysporum f. sp. zingiberi is the causal agent of dry rot on ginger and could highly effect ginger production. Resistant variety is one of disease control strategy that is recommended. Various ginger varieties are available but their response to F. oxysporum f. sp. zingiberi has not been reported. In this study three ginger varieties were evaluated for their resistence to F. oxysporum f. sp. zingiberi based on in vitro and in planta inoculalation method. Gadjah and Emprit varieties were considered moderately susceptible whereas Merah varieties was moderately resistance to F. oxysporum f. sp. zingiberi. pathogen will be indicated.Key words: dry rot disease, ginger var. Merah, resistant variety, resistance

Ketahanan Galur Padi Hibrida Potensi Hasil Tinggi terhadap Penyakit Tungro Ifa Manzila; Tri Puji Priyatno; Ida Hanarida
Jurnal Fitopatologi Indonesia Vol 9 No 3 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

ABSTRACT Wild rice Oryza rufipogon accession can be used as a source of resistance genes to develop elite rice varieties for Rice tungro virus. This study aimed to examine some potential high yield rice lines developed by crossing IR64 with O. rufipogon for their response to three isolates of Rice tungro virus originating from Bogor, Sumedang, and Bali. Virus transmission was done by insect vector, Nephottetix virescens. Variation on plant response was observed. Three lines i.e. Bio5-AC-Blas/BLB, Bio62-AC-Blas/BLB-03, Bio111-BC-Pir7, showed stabile resistance response to all isolates of Rice tungro virus; 6 lines i.e. Bio132-AC2-Blas, Bio138-AC2-Blas, Bio148-Mamol-Dro, Bio154-Mamol-Dro, Bio159-Mamol-Dro, Bio 153-Mamol-Dro were moderately resistance. Virus isolates from Sumedang and Bali is more virulence than isolate from Bogor based on observation on incubation period, disease severity and suppression of plant height.

Minyak Nilam sebagai Biofungisida untuk Pengendalian Penyakit Antraknosa Cabai Desni Roha Miriam Sakerebau; Bonny Poernomo Wahyu Soekarno Poernomo Wahyu Soekarno
Jurnal Fitopatologi Indonesia Vol 9 No 3 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Anthracnose caused by C. capsici is the most important factor causing low yield on chili in Indonesia. A research was conducted to examine to examine the potential of patchouli oil in suppressing anthracnose disease development on chili. In vitro test was conducted by growing C. capsici on potato dextrose agar (PDA) medium that has been added with different concentration of patchouli oil, i.e. 10, 5, 2.5, 1.25 and 0.625%. Negative controls treatment (Kn) was conducted by growing C. capsici on PDA without patchouli oil, whereas positive controls (Kp) was conducted by growing C. capsici on PDA containing mancozeb fungicide. Patchouli oil -A significantly suppressed the growth of C. capsici compared to patchouli oil-B on 10 days after harvest, i.e. 91.15% suppression for all concentration level except for 0.625% which caused 85.92% suppression. Further examination using patchouli oil-A showed that incubation period on curative test was 8 days after inoculation (DAI) whereas on preventive and induced resistance test were 5 DAI. Disease severity at 10 DAI reached 4.8%, 23.20%, and 29.60% on curative, preventive, and induced resistance tests, respectively. Disease severity was 29.60% and 68.80% on positive control and negative control, respectively.

Pengendalian Penyakit Budok dengan Fungisida dan Deteksi Residu pada Daun Nilam Christanti Sumardiyono; Sedyo Hartono; Nasrun Nasrun; Sukamto Sukamto
Jurnal Fitopatologi Indonesia Vol 9 No 3 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Budok caused by Synchytrium pogostemonis is the important and destructive disease on patchouli plant (Pogostemon cablin). Chemical control must be done in incidence with high disease intensity and prediction of outbreak. The experiment was done using benomyl (Benlate 50 WP) and cuprous oxide (Kocide 77WP) 0.1%, 0.2% and mixture of both ( 1 g cuprous oxide and 1 g L-1 benomyl). Patchouli plant was sprayed eight times with one week interval. Harvesting was done two weeks after the end of spraying. Residue analysis of Cu was done with AAS and benomyl residue with HPLC. The result showed that 0.1% benomyl significantly reduced the disease intensity. The other treatments showed lower result. Harvested patchouli plants treated with 0.1% benomyl showed higher yield compare with 0.2% benomyl and cuprous oxyde. Cu residue in dried leaves was between 460 ppm and 950 ppm. Residue of benomyl was 54–100 ppb. Diseased seedling still can be used after spraying by benomyl four times with 2 weeks interval.

Penyakit Kering Pelepah pada Tanaman Kelapa Sawit di Provinsi Kalimantan Timur dan Sumatera Utara Donnarina Simanjuntak; Agus Susanto
Jurnal Fitopatologi Indonesia Vol 9 No 3 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

The rapid development of oil palm industry is indicated by wide monoculture plantation. This condition causes the development of many diseases such as basal stem rot, bud rot, leaf spot, anthracnose, and fruit rot diseases. Recently, a new disease causing dry frond symptom was reported from plantations in 2 provinces, i.e. East Kalimantan and North Sumatera. Disease exploration was conducted in Muara Wahau estate, East Kalimantan and Kota Pinang (Labuhan Batu) estate, North Sumatera. Typical symptoms involved decaying which started from the tip of frond that spread into the middle, rotting brown to blackish; when rot had reached the middle, the frond becomes dry, and finally broken. Isolation and identification of the fungi from the infected plant showed the association of Thielaviopsis sp., although Koch Postulate–based assay was unable to prove that the disease was caused by infection of Thielaviopsis sp.

Penggunaan Bakteriofag untuk Kit Detektor Patogen Hawar Bakteri Kedelai Nurul Rama Dhany; Hardian Susilo Addy; Wiwiek Sri Wahyuni
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Bacterial blight disease on soybean caused by Pseudomonas syringae pv. glycinea is an important factor causing yield loss in Indonesia. Bacteriophage isolated from irrigation water around the soybean field can be used as indicator for the presence of phytopathogenic bacteria. The objectives of this research was to obtain suitable composition of detector materials to detect P. syringae pv. glycinea using bacteriophage. Composition of detector kit contains of nutrient broth medium with 0.1% of bromothymol blue, 10 g talk and 1 g CMC which will be rubbed on to the detector paper and caused green colour development (pH ± 7) when the paper was dipped on to bacteria suspension, added by a drop of bacteriophage suspension and incubated for 24 hour, the colour will be changed. Yellow color indicated growth activity of P. syringae pv. glycinea where as blue colour indicated suppression of P. syringae pv. glycinea.

Ganoderma boninense Penyebab Penyakit Busuk Batang Atas Kelapa Sawit Agus Susanto; Agus Eko Prasetyo; Hari Priwiratama; Sri Wening; Surianto Surianto
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Basal stem rot disease (BSR) caused by Ganoderma boninense is the major pathogen of oil palm in Indonesia and Malaysia. Sometimes in the same location, especially in the peatland, upper stem rot (USR) disease is also found. This research was carried out to identify the pathogen of upper stem rot by molecular approach. Fruiting bodies of fungus were collected from 3 plants with upper stem rot symptoms and 2 plants with basal stem rot symptoms from Labuhan Batu North Sumatra and Kampar Riau. Results showed that all of the fruiting body samples taken from plants with upper stem rot and basal stem rot symptoms were G. boninense.

Tomato yellow leaf curl Kanchanaburi virus Penyebab Penyakit Mosaik Kuning pada Tanaman Terung di Jawa Tega Kintasari; Dwi Wiyati Nurul Septariani; Sri Sulandari; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Yellow and yellow mosaic symptoms was observed on eggplant (Solanum melongena) around Bogor, Bandung, Pati, Rembang, and Bantul during a survey in early 2013. Polymerase chain reaction based detection was carried out using total DNA isolated from symptomatic leaf samples and a pair of Begomovirus-universal primers, SPG1/SPG2. The expected size (~900 bp) amplicon was detected from all five symptomatic samples, indicating the presence of Begomovirus infection. Each amplicon was sequenced followed by basic local alignment search tool analysis. It was revealed that the sequence had the highest homology (98.8%) with Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) sequence. This is the first report on TYLCKaV infection on eggplant in Indonesia.

Page 5 of 37 | Total Record : 361

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