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INDONESIA
journal of Basic Medical Veterinary
Published by Universitas Airlangga
ISSN : 23026820     EISSN : 27749959     DOI : https://dx.doi.org/10.20473
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
This journal published original articles, review articles, and case studies in Indonesian or English, in the scope of JBMV has a broad coverage of relevant topics across veterinary basic medical sciences which includes: preclinical and paraclinical disciplines like Anatomy, Physiology, Biochemistry, Pathology, Microbiology, Pharmacology and Medical Education.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 57 Documents
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EFEKTIVITAS PEMBERIAN NANOPARTIKEL EKSTRAK DAUN JUWET (Syzygium cumini) SEBAGAI ADJUVANT TERAPI TERHADAP GAMBARANHISTOPATOLOGI PULMO MENCIT (Mus musculus) YANG DIINFEKSI Plasmodium bhergei Zerlinda Dyah Ayu; Lilik Maslachah; Koesnoto Supranianondo; Endang Suprihati; Hani Plumeriastuti; Rahmi Sugihartuti
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21091

Abstract

The purpose of this study was to learn how to use juwet nanoparticle leaf extract (Syzygium cumini) as an adjunct therapy in pulmonary histopathological mice (Mus musculus) supported by Plasmodium berghei. This study used 30 rats divided into six groups consisting of K0 (disagree without agreement), P1 (fight without agreement), P2 (fight and dispute with chloroquine), P3 (fight and talk with juwet leaf extract), P4 (useful and useful with juwet leaf extract)) and P5 (extracts and combinations given with chloropine and juwet leaf extract nanoparticles) protected by 1x105 in 0.2 ml of Plasmodium berghei. Data were analyzed by Kruskal Wallis using SPSS and continued with the Mann-Whitney test. The results of this study showed that there were significant differences (p <0.05) between P2 and P5, but did not show a significant difference (p> 0.05) with P1, P3 and P4. Based on this study, can be witnessed by juwet nanoparticles (Syzygium Cumini) additional therapeutic leaf extract which is expected to reduce pulmonary damage (hemmoragi, alveolar congestion, edema, hemosiderin) mice (Mus musculus) infected with Plasmodium berghei. 
PENGUJIAN VAKSIN HEPATITIS B FASE SUBKRONIS TERHADAP BERAT ORGAN DAN DIAMETER PULPA PUTIH LIMPA TIKUS PUTIH (Rattus norvegicus) Afifatus Anindya; Kuncoro Puguh Santoso; Fedik Abdul Rantam; Kadek Rachmawati; Chairul Anwar Nidom; Thomas Valentinus Widiyatno; Hani Plumeriastuti
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20410

Abstract

This study aims to determine the effect of hepatitis B vaccine of spleen weight and white pulp diameter in rat (Rattus norvegicus). This study used 20 male wistar rats aged 8 weeks with an average weight of 200-250 grams, which was divided into four groups. Control group (P0) was given PBS solution, treatment group 1 (P1) was given type 1 hepatitis B vaccine, treatment group 2 was given type 2 hepatitis B vaccine, and treatment group 3 (P3) was given type 3 hepatitis B vaccine. Dose administered 0.4 ml and given repeatedly at days 0,30, and 60. Animals model to euthanize on day 75 for sampling. The spleen organ is weighed after the necropsing process and then the spleen is stored for the preparation of histopathologic with HE staining (Hematoxylin Eosin). The data obtained are weight data of spleen organ and spleen white pulp diameter. The results showed the highest weight of spleen organ 0.92 gram in group P0 and the largest white pulp diameter 34,34 μm in group P1. The results of data analyzed using one-way ANOVA (Analysis of Variance) and continued with Duncan alpha test. One Way Anova and Duncan alpha test showed that there were non significantly different between groups (p>0.05). The conclusion is we know that hepatitis B vaccine type 1,2, and 3 can’t give the effect for spleen weight and white pulp diameter in rat (Rattus norvegicus).
HISTOPATHOLOGICAL APPEARANCE OF THYMUS ON BROILER UNDER CHRONIC HEAT STRESS Ratna Andriani; Arimbi Arimbi; Dadik Rahardjo; Hani Plumeriastuti; Djoko Legowo; Eka Pramyrtha Hestianah
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19987

Abstract

The aim of this research was to know histopathological change of broiler’s thymus which exposed by chronic heat stress. The results revealed that there were negative impact on immune organ (thymus). Broiler that exposed to chronic heat stress showed significantly increased number of heterophil and apoptosis histiocytes and cell depletion. There were 20 broilers that divided into 2 groups, each group consist of 10 broiler. Control group (P0) reared at temperature 24-28oC and humidity 40-55% and Heat Stress group (P1) reared at temperature 36-40oC on the litter housing system at Experimental Animals Laboratory, Faculty of Veterinary Medicine, Universitas Airlangga. Before the treatments, broiler had been reared from day-old-chicks to 21-day-old, then continued with heat stress condition from day 22 to 42. Thymus was collected on day 42 to examine histopathological change of thymus and analyzed using Mann-Whitney Test. The results showed significant difference (P<0.05) between Control group and HS (Heat Stress) group by increased number of heterophil, apoptosis histiocytes and cell depletion.
PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP PENURUNAN KADAR MALONDIALDEHID (MDA) PARU MENCIT JANTAN (Mus musculus) YANG DIPAPAR ASAP ROKOK Sapna Kurnia Dewi; Widya Paramitha Lokapirnasari; Dewa Ketut Meles; Imam Mustofa; Maslichah Mafruchati; Iwan Sahrial Hamid
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20416

Abstract

This research aimed to determine the effect of kepok banana peel extract (Musa acuminata) given by per oral in decreasing levels of malondialdehyde (MDA) in lung of male mice (Mus musculus) which was exposed to cigarette smoke. The 24 experimental male mice were divided into 6 groups. The therapy was given for 14 days, all mice were sacrificed and lung MDA level were examined with thiobarbituricacid (TBA) methods. The result of the data analysis test using one-way ANOVA (Analysis of Variance) showed that there were significant differences between the group of animals that exposed to cigarette smoke ( = 840.50± 186.47 nmol / g) and the group of animals that were given with extract of kepok banana peel 56 mg / kg bw  ( = 6471.25 ± 69.25 nmol/g). Besides that, there was an significant difference between the groups of animals that were exposed cigarette smoke ( = 840.50± 186.47 nmol / g) with the group of animals that were given with vitamin C 260 mg/kg bw ( = 555.25± 61.16 nmol / g). It could be concluded that extract of kepok banana peel and vitamin C could decrease MDA levels in lung of male mice which was exposed cigarette smoke. The increased therapy dose gave affect in lowering levels of malondialdehyde in lung.
PEMBERIAN EKSTRAK MENIRAN (Phyllanthus niruri Linn) TERHADAP GAMBARAN HISTOPATOLOGI INFUNDIBULUM AYAM PETELUR YANG DIINFEKSI Escherichia coli Adinda Riezki Irawan; Emy Koestanti Sabdoningrum; Sri Hidanah; Sri Chusniati; Sri Pantja Madyawati; Benjamin Christoffel Tehupuring
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19992

Abstract

The purpose of this research aimed to prove the effect of meniran extract (Phyllanthus niruri Linn) on layer hen infected with Escherichia coli to reduce inflammation and congestion on infundibulum histopathological view.  This research used 20 samples of layer hen. The treatment in this research was P0- (not infected with Esherichia coli and did not treated with meniran(Phyllanthus niruri Linn)), P0+ (infected with Escherichia coli and did not treated with meniran(Phyllanthus niruri Linn)), P1 (infected with Escherichia coli and treated with 10% meniran extract (Phyllanthus niruri Linn)), P2 (infected with Escherichia coli and treated with 20% meniran extract (Phyllanthus niruri Linn)), P3 (infected with Escherichia coli and treated with 30% meniran extract (Phyllanthus niruri Linn)). The data of this study analyzed by Kruskal-wallis and followed with Mann-withney test. The result of this research can be concluded that 10% concentration of meniran extract (Phyllanthus niruri Linn) was effective to eliminate Escherichia coli, 20% concentration of meniran extract (Phyllanthus niruri Linn) able to promote antibacterial activity, and 30% concentration of meniran extract shown antibacterial rate on high potential so it can be used to reduce inflammation and congestion as the result of Escherichia coli infection.
GAMBARAN PATOLOGI HEPAR IKAN LELE DUMBO (Clarias gariepinus) YANG DIPAPAR LOGAM TIMBAL NITRAT Pb(NO3)2 Yolanda Dellavia; Thomas Valentinus Widiyatno; Boedi Setiawan; Arimbi Arimbi; Bambang Poernomo; Yeni Dhamayanti
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21092

Abstract

This research aimed to know the changed damage of liver African catfish (Clarias gariepinus) were exposed by lead nitrat. This study used twenty four of African catfish (Clarias gariepinus) with average weight of 20-25 grams, size 10-12 cm, age ± two months. This research was designed by a completely randomized design (CRD). All member of population of the African catfish were divided into six groups, consist of six repetitions each, namely P1, P2, P3, and P0 as a negative control respectively.  P1 was given with dosage of lead nitrat 7,26 mg/liter, P2 was given with dosage of lead nitrat 14,53 mg/liter, and P3 was given with dosage of lead nitrat 29,06 mg/liter. According to the macroscopic observed, show that liver became swollen and pale.  The histopathological features of hepar were examined under light microscope in 400 times magnification. Scoring method were using Bernet Scoring Method to examined the presence of degeneretion, congesti, necrotic, and infiltration of leukocyte. Then, Kruskal-Wallis test through with Mann-Whitney test of statistical analysis. The statistical analysis showed the median number of P0 (0,05), P1 (0,45), P2 (1,10), and P3 (1,40) respectively. From the result can be concluded lead exposure with dose 29,06 mg/liter severe which was heavily histopatological in hepatocytes cell of african catfish (Clarias gariepinus) liver.
HISTOPATHOLOGICAL CHANGES OF KIDNEY OF BROILER CHICKEN EXPOSED TO CHRONIC HEAT STRESS Irene Teh Kai Xin; Hani Plumeriastuti; Chairul Anwar; Kadek Rachmawati; Suzanita Utama; Djoko Legowo
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20411

Abstract

The aim of this research was to know histopathological changes of kidney of broiler chicken exposed to chronic heat stress. Twenty broilers were divided randomly into 2 groups, 10 broilers each are being exposed to chronic heat stress for 21 days. The first 21 days were the adaptation period in a chamber with temperature 24-28o C and humidity 40-55%. After 21 days, continue with exposure to heat stress in a chamber with temperature 36-40o C and humidity 50-65% in 8 hours per day. After exposed to chronic heat stress, kidney tissues were processed, and kidney tissue histopathological changes were evaluated by using the Klopfleisch modified scoring method. The data was analyzed by Mann – Whitney Test. The result of this research showed that chronic heat stress exposure causing the presence of degeneration of tubular epithelial cell, necrosis of tubular epithelial cell, necrosis of glomerular and interstitial infiltration. From the analysis data the overviewof multiparametric showed that when control group compared with the treatment group showed significantly difference (p<0.05). 
PENGARUH PEMBERIAN PROPOLIS LEBAH TERHADAP GAMBARAN HISTOPATOLOGI HEPAR MENCIT (Mus musculus) BETINA YANG DIPAPAR LOGAM BERAT PB ASETAT [Pb(C2H3O2)2] Sartika Dewi Rachmani; Erma Safitri; Roesno Darsono; Eka Pramyrtha Hestianah; Hani Plumeriastuti; Kuncoro Puguh Santoso
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19988

Abstract

Lead acetate in the hepatobiliary system may cause peroxidation catalysis of unsaturated fatty acids, reduce nitrogenoxide and increase hydroxyl radical. Lead acetate produces oxidative stress characterized by free radical formation and inhibits lipid peroxidation. Giving antioxidants can neutralize free radicals from the detrimental effects that arise on the process or excess oxidation reactions. The purpose of this research was to find out how the effect of bee propolis on histopathologic images of hepatic mice (Mus musculus) of females exposed to lead acetate [Pb(C2H3O2)2]. The subjects were 25 mice (Mus musculus) mushulus of BALB/C strain with average weight 25-30 gram and 8 weeks old, divided into 5 treatment groups, each consisting of 5 heads per group. The K- group was given a Tween 80 solution at a doses of 0.5 mg/kgBW for 20 consecutive days. The K+ group, which was given only lead acetate at a doses of 10 mg/kgBW orally for 10 days. P1, P2, and P3 were given 10 mg/kgBW lead acetate solution orally for 10 days. The following 10 days were given bee propolis with doses of P1 200 mg/kgBW, P2 400 mg/kgBW, and P3 800 mg/kgBW. On the 21th days the mice were dissected, to observe the extent of the damage. All data were performed using a statistical test with Kruskal Wallis test and if there was a marked difference between treatment groups (p<0.05), then the Mann-Whitney test was followed. The results obtained that bee propolis can repair hepatic cell damage in mice (Mus musculus) of females exposed to lead acetate. Increased dose of bee propolis is ineffective in repairing hepatic cell damage in mice (Mus musculus) of females exposed to lead acetate.
STIMULASI TITIK REPRODUKSI DENGAN LASERPUNKTUR SEMIKONDUKTOR TERHADAP PENAMPILAN BIRAHI SAPI BALI (Bos sondaicus) Hanifah Khairun Nisa; Budiarto Budiarto; Tatang Santanu Adikara; Herry Agoes Hermadi; Soeharsono Soeharsono; Imam Mustofa
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21088

Abstract

This research to know the stimulation of reproduction point with a semiconductor laserpuncture to estrous synchronization of Bali Cattle was conducted in Loka Penelitian Sapi Potong Grati-Pasuruan, East Java. Estrous synchronization is a technique to get the sign of estrous and ovulation (estrous outside of estrous cycle) to be easier to detect estrous, synchronization of breeding, and shorter of calving interval. Laserpuncture was applied on 8 head bali cattles at 14 reproduction acupuncture points, 10 second for each point, 3 times frequency with interval 1 day (20-24 times). The variabel of this assessment were respons and signs of estrous, synchronized of estrous with Chi square test and T-test method. Result showed that there was significant correlation between semiconductor laserpuncture with estrous synchronization (p < 0,05). The result of assessment 8 (100%) head bali cattle was estrous. The respons of estrous appeared 1 head (12,5%) on 5th day (4th day post laserpuncture), 4 head (50%) on 6th day (5th day post laserpuncture) 1 head (12,5) on 8th day (7th day post laserpuncture), and 2 head (25%) on 10th day (9th day post laserpuncture). This result showed that semi conductor laserpuncture technology was effective to stimulate estrous synchronization and resulting ovulation, with the evidence that conception was occurred.
EFEK PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL LEYDIG MENCIT (Mus musculus) JANTAN YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Milla Nursadida; Hani Plumeriastuti; Yeni Dhamayanti; Widjiati Widjiati; Epy Muhammad Luqman; Arimbi Arimbi
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19998

Abstract

This study was aim to examine the effect of kebar grass extract (Biophytum petersianum Klotzsch) to against number leydig cells of mice (Mus musculus) by exposed 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Twenty five male mice (Mus musculus) 4 months with avarage body weight 20 g were used. These animals were divided into five groups (K(-), K(+), P1, P2 and P3). K(-) was treated with placebo, K(+) was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw, P1 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,045 mg/g Bw/day P2 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,080 mg/g Bw/day, P3 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 1,350 mg/g Bw/day. This research has been conducted for 53 days. The data were compared using ANOVA and Duncan test by SPSS 22.4 for windows. The result showed that Kebar Grass Extract in all of groups can prevent the damage of leydig cells in testis that exposed by TCCD significantly (p<0,05) and kebar grass extract 0,135 mg/kgBw/day can increase amount of leydig cells maximaly.

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