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Rachmat Hidayat
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Bioscientia Medicina : Journal of Biomedicine and Translational Research
Published by HM Publisher
ISSN : -     EISSN : 25980580     DOI : https://doi.org/10.37275/bsm
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
This journal welcomes the submission of articles that offering a sensible transfer of basic research to applied clinical medicine. BioScientia Medicina covers the latest developments in various fields of biomedicine with special attention to : 1.Rhemumatology 2.Molecular aspect of Indonesia Traditional Herb 3.Cardiology and Cardiovascular diseases 4.Genetics 5.Immunology 6.Environmental health 7.Toxicology 8. Neurology 9. Pharmacology 10. Oncology 11. Other multidisciplinary studies related medicine. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 597 Documents
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Sleep and Bone Density: A Study on Postmenopausal Indonesian Women Shafira Irmayati; Muhammad Reagan; Legiran Legiran
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 1 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i1.68

Abstract

Background: Low bone density has been known as one of the factors that increased fracture risk. Aging, estrogen deficiency, low BMI, and inadequate calcium intake are known factors that contribute to decreased bone density. Other than the known factors, some research show that sleep duration can also lower bone density. Sleep affects bone density in a way that it causes increased level of cortisol, proinflammatory cytokine, and decreased in physical activity. Method: This study is an observational analytic study with cross-sectional design. It is conducted on menopausal patients that has been examined with bone mineral densitometry at Poliklinik Reumatologi Penyakit Dalam RSUP Dr. Mohammad Hoesin Palembang in November 2017 to Oktober 2018. Samples are collected using total sampling technique. Results: From 93 subjects that participated in this study, 20,4% of them had osteopenia, and 51,6% had osteoporosis. Patients with low bone density, mainly slept for 6 to 8 hours at night per day, 30 minutes of nap per day, and has a total sleep duration for less than 8 hours each day. This study shows no significant association between night-time sleep, daytime napping, and total sleep duration with bone density (p value = 0,168, p value = 0,831, p value = 0,984). Analysis on other risk factors show significant association between body mass index and low bone density (p value = 0,002). Conclusion: There are no significant association between sleep duration and bone density in menopausal patients at Poliklinik Reumatologi RSUP Dr. Mohammad Hoesin Palembang.
The Effect of Ischemic-Reperfusion on Acute Kidney Injury Pathogenesis to the Glomerular Microscopic Appearance and Cystatin C Level in Wistar White Rat. Annisa Wimaulia Azlin; Rachmat Hidayat; Kemas Ya'kub Rahadiyanto
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 1 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i1.79

Abstract

Acute kidney injury (AKI) is a sudden decrease of kidney function. The incidence of AKI is increasing every year. One of the causes of AKI is the lack of blood supply to the kidneys (prerenal). At the present time, there are not many studies that report unilateral ischemic-reperfusion (UIR) duration which can cause kidney damage especially glomerulus. The aim of this study is to determine the effect of UIR duration to glomerular microscopic appearance, GFR and cystatin C produced in Wistar white rats. This study was performed in vivo by using Post-test Only Control Group Design. Unilateral Ischemic-Reperfusion was administered on the rat’s left kidney and recovery was done according to specified time. After recovery time, rat’s blood was taken, the rat was euthanized, and its kidneys were taken and stained with Picrosirius Red coloring. The kidneys were observed by using OptiLED and the photos were analyzed with ImageJ software. Blood samples were tested by ELISA to measure the cystatin C levels and the levels were converted into Larrson formula to obtain Glomerular Filtration Rate. The level of cystatin C increased along with the longer duration of UIR and compared inversely proportional to GFR which decreased along with the rise of UIR duration. Cystatin C and GFR had a significant mean difference (p<0.05) with all groups, except for the duration of the UIR group <60 minutes. The percentage of collagen obtained fluctuated but the whole group which was carried out by UIR had a significantly different collagen amount (p <0.05) with the sham-operated group. The average glomerular picture showed the addition of collagen, Bowman's capsule thickening and vascular retraction. The longer duration of UIR will worsen the kidney function. Keywords: Unilateral Ischemic-Reperfusion, Cystatin C. Glomerular Filtration Rate, Collagen Area Fraction, Glomerulus
The Relationships between Neutrophil/Lymphocyte Ratio and Child-Turcotte-Pugh Classification in Assessing Severity of Liver Cirrhosis Griselda Griselda; Kemas Ya'Kub Rahadiyanto; Rachmat Hidayat
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 1 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i1.80

Abstract

Background Liver cirrhosis is a disease with high morbidity and mortality in Indonesia. The Child-Turcotte-Pugh classification is a noninvasive parameter to assess severity of liver cirrhosis. Meanwhile, the neutrophil/lymphocyte ratio can reflect person's immunity to liver cirrhosis. This study was conducted to determine the agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification in assessing severity of liver cirrhosis. Methods Analytical observational study with Cohen’s Kappa agreement test was conducted in 86 liver cirrhosis patients. Data was retrieved by observing the medical records. Neutrophil count, lymphocyte count, hepatic encephalopathy, ascites, albumin, bilirubin, and INR were all recorded. Documented variables were analyzed by the Cohen’s Kappa agreement test. Results In Cohen’s Kappa agreement test, there was moderate agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification in all samples (κ = 0.591 and p <0.001). Agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification based on age and gender was found to be κ = 0.777 at age ≤45 years, κ = 0.532 at age >45 years, κ = 0.682 in male, and κ = 0.445 in female (p <0.001). The highest agreement is the substantial agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification obtained at age ≤45 years (κ = 0.777 and p <0.001) and male (κ = 0.682 and p <0.001). Conclusion There is moderate agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification in assessing severity of liver cirrhosis. Substantial agreement obtained at age ≤45 years and male gender. Keywords: Neutrophil/Lymphocyte Ratio, Child-Turcotte-Pugh, Liver Cirrhosis
Association of Stress Level with Menstrual Disturbance Among Female Students in Medical Faculty Sriwijaya University Fildzah Hashifah Taufiq; Herry Hasnawi; Rachmat Hidayat
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 1 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i1.82

Abstract

Association Of Stress Level With Menstrual Cycle Disturbance Among Female Students In Medical Faculty Of Sriwijaya University. Menstruation occurs regularly every month will form a menstrual cycle. Normal menstrual cycles in women range from 21-35 days. However, the menstrual cycle is not always normal, many women experience menstrual disturbance. Menstrual cycle disturbance are caused by several factors, which is stress. This study aims to analyze the association of stress level with menstrual cycle disturbance among female students in Medical Faculty of Sriwijaya University. This study is analytical cross-sectional study with primary data from menstrual cycle, stress ISMA, personality type DSM V questionnaires.. In this study, 503 samples are fulfilled inclusion criteria. Among 503 subjects, 59 (11.7%) female students experienced polymenorrhea, 391 (77.7%) female students had normal menstrual cycles, 53 (10.6%) female students experienced olygomenorrhea. Chi Square test showed that there was an association between stress level and menstrual cycle disturbance. There is a significant association between between stress level and menstrual cycle disturbance among female students in Medical Faculty of Sriwijaya University. Keywords: Stress level, Menstrual cycle disturbance.
Body Mass Index as a Parameter of Running Speed indri seta septadina; Tri Suciati; Ha Sakinah Se
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 2 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i2.83

Abstract

Speed is determined by several factors such as anatomical and physiological factors. Body Mass Index (BMI) is one of an indicator from anthropometric measurement that is relevant to distinguish the capacity and performance of athletes. The aim of this study is to investigate the correlation between BMI and running speed. It was an observational research with cross-sectional design of 35 students of Athlete High School in Palembang. students. The primary data was taken from anthropometric measurements (stature meters, weight scales, meters) and assessment of 50 meters running speed tests. Data analysis was executed by using Pearson or Spearman correlation test in SPSS. The BMI of subjects varied from 17.05 to 27.12 with mean 21.48. The running speed of subjects varied from 5.67 to 8.17 m/s, with an average of 6.78 m/s. The bivariate analysis showed that there was a weak negative correlation between BMI and running speed (r = -0160). There was a weak negative correlation between BMI and running speed (r = -0160). A negative correlation means that the greater BMI then the less time it takes to run at a distance of 50 meters, in other words, the better performance of athletes.
Antibacterial Activity of Cinnamon Extract (Cinnamomum burmannii) against Staphylococcus aureus and Escherichia coli In Vitro Nita Parisa; Rahma Nur Islami; Ella Amalia; Mariana Mariana; Riana Sari Puspita Rasyid
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 2 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i2.85

Abstract

Abstract Infectious disease is one of the most common diseases in the world. Staphylococcus aureus and Escherichia coli are two common causes of infection and are resistant to many antibiotics, so the new agents are needed to overcome antibiotic resistance. Cinnamon is often used as a preservative because it has antibacterial activity. Cinnamomum burmannii is kind of native cinnamon from Indonesia. The antimicrobial active compounds cinnamaldehyde and eugenol are the main reasons for its antibacterial activity. This study observed the efficacy of the cinnamon extract (Cinnamomum burmannii) as antibacterial against Staphylococcus aureus and Escherichia coli. An experimental study, in vitro using Post-test Only Control Group Designed, has been done in Microbiology and Biotechnology Laboratory of Medical Faculty of Sriwijaya University. Cinnamon was extracted, then tested for its antibacterial activity using well diffusion and serial dilution to determine diameter of inhibition zone and minimum bactericidal concentration. Phytochemical tests were also conducted to determine the antibacterial compounds of cinnamon extract. Ethanol extract of cinnamon was able to inhibit the growth of Staphylococcus aureus with MBC 5% and inihibitory zone 6,84±0,68 mm and Escherichia coli with MBC 10% and inhibitory zone 5,69±0,69 mm. Cinnamon extract which has the greatest effectiveness is concentration of 40% with inhibition zone 15,69±0,80 mm (Staphylococcus aureus) and 9,63±0,59 mm (Escherichia coli). This ability is due to the antibacterial compounds as evidenced by positive results in various phytochemical tests. Cinnamon extract is effective as antibacterial against Staphylococcus aureus and Escherichia coli in vitro. Keywords: efficacy, antibacterial, Cinnamomum burmannii, Staphylococcus aureus, Escherichia coli
A Hospital-Based Study: Interventions for Patients with Disorders of Sex Development (DSD) in National Reference Hospital, South Sumatera Region, Indonesia Ziske Maritska; Bintang Arroyantri Prananjaya; Andhika Diaz Maulana
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 2 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i2.86

Abstract

ABSTRACT Background. Disorders of Sex Development (DSD) is a term used to describe a congenital condition where sex chromosomes, gonads, and or anatomy of a person are atypical. It is estimated to affect 1:4,500 people worlwide with varied clinical findings, hence the integrated and diverse interventions. Objective. This study wished to observe the many interventions DSD patients received in Dr. Mohammad Hoesin Hospital as one of the national reference hospitals in Indonesia for South Sumatera region. Methods. Medical records of 173 patients with clinical findings in accordance with DSD spectrum during 2013-2017 was observed in order to identify both surgical and medical interventions applied. Results. Sixty two patients were diagnosed of having hypospadias (35.84%), making it the most common clinical finding among DSD patients in this hospital. Surgical intervention was the most favored intervention, with 155 patients (89.6%) underwent various surgeries, ranging from urethroplasty to vaginoplasty and many more. Five patients(2.9%) were treated with either hydrocortisone and florinef, or both. One patient diagnosed with Congenital Adrenal Hyperplasia (CAH) had both surgical and medical intervention. Conclusion. DSD gives a wide phenotypic spectrum, making its management both challenging and complicated. Surgical, medical, and or a combination of both are the indispensable interventions when dealing with DSD patients. Study focusing on psychological intervention for DSD patients is further needed. Keyword: Disorders of Sex Development, DSD, surgical, medical, intervention.
Antibacterial Efficacy of Aloe vera Sap Against Staphylococcus aureus and Escherichia coli Safira Azahra; Nita Parisa; Fatmawati Fatmawati; Ella Amalia; Venny Larasati
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 2 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i2.87

Abstract

Abstract Background Aloe vera is a plant that has been used as an alternative drug. This plant contains various compounds, like anthraquinone, saponin, flavonoid, alkaloid, and tannin that has an antibacterial effect against Staphylococcus aureus and Escherichia coli. Both of it were responsible for the infection incident. This study aims to determine the efficacy of Aloe vera sap as an antibacterial against Staphylococcus aureus and Escherichia coli. Methods An experimental study, in vitro using post-test only control group design, has been done at laboratory of Medical Faculty of Sriwijaya University, by examining the antibacterial activity of Aloe vera sap in five different concentration (5%, 10%, 20%, 40%, dan 80%) using well diffusion and solid dillusion method to determine the minimum bactericidal concentration (MBC). And then continued with the phytocemical screening to determine the compound inside the Aloe vera sap. Results Aloe vera sap were able to kill Staphylococcus aureus at 5% and Eshcerichia coli at 80%. Compatibility test showed that Aloe vera sap with concentration of 10%, 20%, 40%, and 80% are compatible with amoxicillin, therefore 80% is compatible with cefotaxime. This ability due to the compound that it contains, which is alkaloid, flavonoid, tannin, quinone, and saponin. Conclusion Aloe vera sap is effective as an antibacterial against Staphylococcus aureus and Escherichia coli. Keyword: Aloe vera sap, antibacterial, efficacy, Staphylococcus aureus, Escherichia coli.
Biomolecular Aspects of Schizophrenia Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 2 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i2.88

Abstract

Abstract Schizophrenia is a common psychiatric disorder, which is characterized by severe distortion of reality; disturbances in thoughts, feelings and behavior; according to DSM V is a disorder form deviations fundamentals and characteristics of thought and perception, and by the innapropriate or blunted affect. The influence of genetics is believed to have a role in psychiatric disorders, especially if the disorder has occurred in young adults or adolescents. The pathophysiology of schizophrenia is closely related to disorders of the biomolecular aspects of the central nervous system. Dopamine activity in the striatal area and prefrontal cortex is a mechanism believed to be the cause of the emergence of positive and negative symptoms in schizophrenia. Meanwhile, neuronal cell apoptosis and increased oxidants, especially in the basal ganglia and prefrontal cortex areas cause worsening of negative symptoms experienced by schizophrenic patients.
Aphrodisiac Effects of Areca Fruit in Erectile Dysfunction Rat Model Nita Parisa; Muhammad Totong Kamaluddin; Salni Salni
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 3 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i3.90

Abstract

Abstract. Erectile Dysfunction (ED) cases are quite high in both women and men and increase with age. Areca fruit, ginseng and purwoceng are plants that have been traditionally used to increase male vitality. This in vivo experimental research study with pre and post-test control group design located in the laboratory of animal house and the biomolecular laboratory Faculty of Medicine Sriwijaya University. The subjects of this study were albino rats (Rattus norvegicus), Wistar strains, divided into 6 groups are negative control, Sildenafil, Areca Fruit Extract (AFE) 50 mg/kgBW, AFE 100 mg/kgBW, Combination of Areca Fruit, Ginseng and Purwoceng (AGP) 50 mg/kgBW and AGP 100 mg/kgBW. Female rat was injected by giving 500 mg/100 g of subcutaneous progesterone and 10 μg/100 g of BB benzoate 48 hours before mating so that female mice enter the esterus phase. In this study it was found that Combination of Areca fruit, Ginseng and Purwoceng (AGP) group 50mg/kgBW significantly improved testosterone levels compared to sildenafil, while single areca fruit (AFE) dose 50mg/kgBW increased FSH and LH levels as well as sildenafil statistically. Keywords: areca fruit, erectile dysfunction, FSH, ginseng, LH, purwoceng, testosteron.

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