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Contact Name
Sutiman Bambang Sumitro
Contact Email
berkalahayati@yahoo.com
Phone
+62341570631
Journal Mail Official
wulidanisa@berkalahayati.org
Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia
Location
Unknown,
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INDONESIA
Berkala Penelitian Hayati
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Articles 491 Documents
Activity of Mikania micrantha leaf extract against subterranian termite and wood decay Indrayani, Yuliati; Alkhadi, Alkhadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 26 No 2 (2021): June 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1652.098 KB) | DOI: 10.23869/bphjbr.26.2.20211

Abstract

Mikania micrantha is a wild weed that thrives in tropical areas, grows rapidly in areas with high humidity and light intensity as well as in fertile soil. Meanwhile, Indonesia's tropical climate is also a suitable habitat for the breeding of termites and wood decay fungi. This study aims to determine the bioactivity of M. micrantha leaf extract against subterranean termite, Coptotermes curvignathus (Holmgren) and wood decaying fungus, Schizopyllum commune and its optimal extract concentration to prevent termite attack and inhibit fungal growth. The extracts were obtained by drying and mashing of M. micrantha leaves. The leaf powder was sieved with a 40-60 mesh filter and macerated with methanol for 3x24 h. The maceration results were filtered and the filtrate was concentrated using a rotary evaporator. Furthermore, identification of the secondary metabolites of the extract was carried out by phytochemistry. The bioactivity test included anti-subterranean termite and wood decay fungus using Potatos Dextrose Agar (PDA) medium. The results showed that M. micrantha leaf extract affected sample weight loss, termite mortality rates and the growth of wood decay fungi. Furthermore, the concentration of M. micrantha extract was inversely proportional to the sample weight loss and directly proportional to the termite mortality rate. In addition, the concentration of M. micrantha leaf extract had a negative correlation with the growth of the decaying fungus of S. commune therefore, the concentration of the extract was directly proportional to the growth inhibition of fungus. Meanwhile, the 4% extract concentration led to the increase in termite mortality rate above 80% and inhibited fungal growth by 100%. The results suggest that the bio-efficacy data from this study may be used to develop wood protecting systems based on wild weeds which are found in tropical areas.
Test of protease activity on pigeon pea beans (Cajanus cajan (L.) Millsp.) fermented by Aspergillus niger Pujiati, Pujiati; Primiani, Cicilia Novi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 2 (2018): June 2018
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (727.126 KB) | DOI: 10.23869/546

Abstract

The objective of this study is to establish the effect of inoculum concentration and incubation time of protease activity on pigeon pea beans fermented by Aspergillus niger. The study comprises two independent variables: the inoculum concentration of Aspergillus niger, which consists of four levels (0 mL/g; 0.2 mL/g; 0.4 mL/g, and 0.6 mL/g), and six levels of incubation time (0 h, 24 h, 48 h, 72 h, 96 h, and 120 h). The obtained data were tested using two-way analysis of variance (ANOVA) followed by an LSD test with a significance level of 5%. The results show that the inoculum concentration of Aspergillus niger and incubation time do affect the activity of the protease enzyme. The highest enzyme activity of 0.298 U/ml was obtained at an inoculum concentration of Aspergillus niger of 0.4 mL/g, with an incubation time of 96 h (P2K4).
PENGARUH PEMUPUKAN DAN VEGETASI TERHADAP KEBERADAAN JAMUR TANAH DI LAHAN BEKAS PENAMBANGAN EMAS YANG DIREKLAMASI PADA DAERAH CIMANGGU DAN BOJONG PARI JAMPANG SUKABUMI Yulinaeri, Titin; Suciatmih , Suciatmih; Suharna, Nandang
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 7 No 1 (2001): December 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.418 KB) | DOI: 10.23869/509

Abstract

In order to know the effect of vegetation and fertilizer on the occurrence of soil fungi, a study was conducted in reclamated gold mining land both in Cimanggu and Bojong Pari, Jampang, Sukabumi. Soil fungi were isolated by dilution plate method, using a “tauge sucrose agar� medium with 50 ppm antibiotic and incubated at room temperature for 2-3 days. Fungi identification follows Domsch et al (1980), Samson et al (1981), and Barnet (1969).The effect of vegetation and fertilizer on the composition of soil fungi in reclamated land both Cimanggu and Bojong Pari were not quite different. Aspergillys, Eupenicillium, and Pennicillium maybe dominant fungi in these areas. These fungi were probably involved in reclamation of land. To obtain faster reclamation process, the fungi are better involved as one of introduced microorganism besides Rhizobium and Mychorizal fungi or mixed inoculants. Besides election of suitable flora and fauna, soil fungi are expected to be able recover degraded land into original ecosystem.
Evaluation of Bacillus thuringiensis-based bioinsecticide impacts on arthropod biodiversity in the intercropping system Pujiastuti, Yulia
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 1 (2019): December 2019
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.34 KB) | DOI: 10.23869/17

Abstract

The intercropping system is intended to increase vegetable production. However, The presence of pests reducing production levels is a challenging constraint and should be controlled. In this study, Controlling insect pests will be evaluated by Bacillus thuringiensis-based bioinsecticide. The purpose of the research was to investigate the biodiversity of insect pests and their natural enemies in intercropping systems (cucumber and long beans), applied by B. thuringiensis-based bioinsecticide, chemical insecticide, and no-both application. Observation of existing insect-plant canopy was directly observed, and those on soil surface were observed by pitfall traps and yellow trays, as well. The research was performed in the experimental farm of the Sriwijaya University Faculty of Agriculture, Indralaya Campus, Ogan Ilir, South Sumatra, from July until October 2018. Results indicated three ecological roles of insects, namely predators/carnivores, pests/herbivores, and pollinators. Other insects and arthropods obtained from direct observation sampling method identified ten orders, but treatments of B. thuringiensis-based bioinsecticide resulted in 29 families of insects, chemical treatment was 17, and control (no treatment) was 32 families. B. thuringiensis-based bioinsecticides may be used in the intercropping system since it causes the highest biodiversity (H '= 2.96), high predator population (45%) and low pest population (41%) compared to other treatments.
Developmental the pattern of embryogenic microspore of rice (Oryza sativa L.) based on morphological characteristic Nurbaiti, Siti; Purwestri, Yekti Asih; Daryono, Budi Setiadi; Semiarti, Endang; Indrianto, Ari
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 1 (2019): December 2019
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (613.554 KB) | DOI: 10.23869/18

Abstract

Microspore culture is a rapid approach to get double haploid plants in shorten time which is important for plant breeding. Microspore culture of pigmented rice cultivar Segreng was used to observe the pattern of embryogenic development based on the characteristics of morphology. Characterization between embryogenic and non-embryogenic microspores was conducted after exposed anther to carbohydrate starvation in B medium culture at 33oC for 4 days. The result showed that enlarged microspore and star-like structure of fragmented cytoplasm become a marker of embryogenic microspore after stresses treatment. More complex of fragmented microspore was found at 4 days in A2 medium and developed further into multicellular structure. These multicellular structures might be originated either by symmetrical or asymmetrical division. The longer incubation, the higher frequency of complex fragmented microspore that developed into multicellular structure release from exine has appeared.
Phytochemical properties and antioxidant activities of the leaf extracts of Boenninghausenia albiflora (Hook.) Rchb. ex. Meisn. (Rutaceae) Wibawa, I Putu Agus Hendra; Andila, Putri Sri; Saraswaty, Vienna; Tirta, I Gede
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (438.347 KB) | DOI: 10.23869/19

Abstract

Boenninghausenia albiflora is a well-known plant for being used in various traditional treatments. The plant is also reported to contain an essential oil which has a distinctive and pungent aroma. The present study aimed at investigating extraction yields, phytochemical compounds and antioxidants, and potential uses of B. albiflora leaves extracted with acetone solvent. Bioactive compounds of the leaf extract of B. albiflora were identified by GC-MS analysis while the antioxidant assay was measured by DPPH method. The extract obtained from acetone contained 30 compounds including coumarin group, 2-Isoprenyl-2,3-dihydro-furo [3,2-G] chromen-7-one (22.89% w/v) as the highest percentage compound. Other coumarins such as suberosin and dehydrogeijerin were also identified in this study. The IC50 values of the leaves extract of B. albiflora was 194.3 ppm which was higher than propyl gallate. Thus indicating B. albiflora as potential sources of anti-cancer, anti-inflammatory and antioxidant.
Profile of microbial community of naturally fermented Sumbawa mare’s milk using next-generation sequencing Jatmiko, Yoga Dwi; Mustafa, Irfan; Ardyati, Tri
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.54 KB) | DOI: 10.23869/20

Abstract

This study aimed to investigate the bacterial and fungal/yeast diversity in naturally fermented Sumbawa mare’s milk through a next-generation sequencing approach, and evaluate the quality of fermented mare’s milk based on the presence of pathogenic or undesirable microorganisms. Microbial density determined using plate count agar (total aerobic bacteria), de Man Rogosa Sharpe agar (Lactobacillus), M17 agar (Lactococcus) and yeast peptone dextrose agar supplemented with streptomycin 50 ppm (yeast). Nutritional content and acidity level of each fermented milk sample were also evaluated. Genomic DNA was extracted using FastDNA Spin (MPBIO). The total gDNA was further analyzed using illumina high-throughput sequencing (paired-end reads), and the sequence results were analysed using QIIME v.1.9.1 to generate diversity profiles. The difference in nutrient content of mare’s milk was thought to affect the density and diversity of microbes that were able to grow. Fermented mare’s milk samples from Sumbawa had the highest bacterial diversity compared to samples from Bima and Dompu. However, fermented mare’s milk from Dompu had the best quality which was indicated by the absence of bacteria that have the potential to be pathogenic or food spoilage, such as members of the Enterobacteriaceae family (Enterobacter, Klebsiella and Escherichia-Shigella) and Pseudomonas. Genus of Kazachstania and Kluyveromyces, as well as family Dipodascaceae were frequently observed fungi/yeast from Sumbawa fermented mare’s milk. The presence of potential pathogenic bacteria warrants special attention in improving the hygiene of manufacturing process.
Seed storage importance and behaviour of a vulnerable species Angsana (Pterocarpus indicus Willd.) Rindyastuti, Ridesti; Siahaan, Febrina Artauli
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.886 KB) | DOI: 10.23869/21

Abstract

Angsana (Pterocarpus indicus Willd.) is one of woody plant species listed in the IUCN red list for priority in conservation as a vulnerable species. The effective and low-cost methods of seed storage is required to support the ex-situ conservation through seedling recruitments for population improvement. This study used completely randomized factorial design with 2 factors i.e. seed storage methods (room temperature, sealed jar and cold temperature), and storage duration (1,2,3,4 and 5 months). This study used 3 replications for each treatments and 10 seeds for each replication. The result showed that the seed of P. indicus start to germinate in 5-11 days after seeding while leaf emerged in 15-13 days after seeding. Seed viabilities generally were high until 3 months of storage (up to 65%) and start to decline at 4 months of storage at 51%. Storage methods and storage duration significantly influenced the seedling growth, viability, and Relative Growth Rate (RGR). There was an interaction between two factors. The storage at room temperature during a month (1RT) has the highest seedling height. The seeds stored in sealed jar during 5 months (5SJ) showed the highest seedling RGR, while the seeds stored in sealed jar during 3 months (3SJ) has the highest seed viability. For longer term storage, the seed of P. indicus should be stored in sealed jar or at room temperature at 11% moisture content. Both in the rainy and dry season, seedling recruitments were low and sapling stage was rare which showed low seedling establishment. Moreover, based on the phenological information, it is better to store the seed during the rainy season.
Effect of NPK and KNO3 fertilizer on yield and quality of Madura tobacco Prancak N1 Djajadi, Djajadi; Syaputra, Roni
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.672 KB) | DOI: 10.23869/22

Abstract

Madura tobacco Prancak N1 is a new superior variety with higher quality but lower nicotine content than Prancak 95 tobacco variety. The rate and form of fertilizer for the new variety has not yet been defined so that farmers have to use adding nitrogen fertilizer their tobacco with no phosphorus and potassium fertilizer. The objective of the research was to determine effect of NPK and KNO3 fertilizers on yield and quality of Madura tobacco var. Prancak N1. The research was carried out at Cen-lecen village, Pakong Sub District of Pamekasan District, Madura. The experiment was laid out in a randomized block design with three replication of each plot. Treatments consisted of 9 packs of fertilization with NPK compound fertilizer (8-15-19), and KNO3 (13-0-45) and those were compared with 1 packed of recommended using single fertilizer of 40 kg N-ZA + 36 kg P2O5-SP36 + 25 kg K2O-ZK + 5 ton organic manure per hectare. Results showed that addition of compound fertilizers NPK plus KNO3 increased absorption of macro nutrients NPK, yield and quality of madura tobacco Prancak N1 variety. Package fertilizers NPK plus KNO3 with the rate of 40 kg N + 36 kg P2O5 + 61 K2O per hectare was suggested for madura tobacco Prancak N1 variety to produce high yield (846 kg/ha), grade index value (87.4), and crop index value (73.9).
In silico exploration the phenolic compound of olive leaves as acetylcholinesterase enzyme (AChE) inhibitor for Alzheimer’s disease therapy Mubarakati, Nurul Jadid; Puspitarini, Oktavia Rahayu; Rahayu, Tintrim; Maulidiyah, Alik
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.905 KB) | DOI: 10.23869/23

Abstract

Olive (Olea europaea) have been cultivated and grown well in tropical climates such as Indonesia. Indonesia local community have used olive as herbal medicines due to its active compounds known as oleuropein that has many biological activities including as neurotherapy in Alzheimer's disease. The purpose of this study is to explore the potential phenolic compounds of olive and examine the acetylcholinesterase (AChE) inhibitory activity displayed by different olive polyphenols through a silico approach. The bioactive compounds of olive which had been analyzed in this study were phenolic compound included oleuropein, demethyl-oleuropein, ligstroside, oleoside, verbascoside, luteolin 7-glucoside, and hydroxytyrosol. Interaction of bioactive compounds with acetylcholinesterase (AChE) was analyzed through molecular specific docking using AutoDock Vina with Pyrx Software. The result elucidate that olive contain potential biological activities as antioxidant, anti-inflammatory, antineoplastic, free radical scavenger, antibacterial, antifungal, expression TP53 enhancer, caspase 8 stimulant, platelet adhesion inhibitor, treatment for lipoprotein disorder, antiviral and dementia treatment/Alzheimer disease. The highest bioactivity percentage of olive phenolic compound are as an antioxidant of 82%, anti-inflammatory of 73%, and anti-cancer (antineoplastic) of 70% respectively. Based on molecular docking analysis show one of olive phenolic compound of the dimethyl-oleuropein has strong interaction with AChE as pointed in the binding affinity of demethyl-oleuropein +AChE of -8.9 kcal/mol has closed to galanthamine binding affinity of -10.3 kcal/mol. Along with glutamate acid 202, tyrosine 133 and tyrosine 124 are the major contributors in the target-ligand interactions. The selected demethyl-oleuropein ought to be tested in clinical studies to discover new neuro-therapeutic candidates.

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