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Berkala Penelitian Hayati

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Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur

ISSN : 08526834 EISSN : 2337389X DOI : https://doi.org/10.23869/bphjbr

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 507 Documents

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Evaluation of Bacillus thuringiensis-based bioinsecticide impacts on arthropod biodiversity in the intercropping system Yulia Pujiastuti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 1 (2019): December 2019
Publisher : The East Java Biological Society

The intercropping system is intended to increase vegetable production. However, The presence of pests reducing production levels is a challenging constraint and should be controlled. In this study, Controlling insect pests will be evaluated by Bacillus thuringiensis-based bioinsecticide. The purpose of the research was to investigate the biodiversity of insect pests and their natural enemies in intercropping systems (cucumber and long beans), applied by B. thuringiensis-based bioinsecticide, chemical insecticide, and no-both application. Observation of existing insect-plant canopy was directly observed, and those on soil surface were observed by pitfall traps and yellow trays, as well. The research was performed in the experimental farm of the Sriwijaya University Faculty of Agriculture, Indralaya Campus, Ogan Ilir, South Sumatra, from July until October 2018. Results indicated three ecological roles of insects, namely predators/carnivores, pests/herbivores, and pollinators. Other insects and arthropods obtained from direct observation sampling method identified ten orders, but treatments of B. thuringiensis-based bioinsecticide resulted in 29 families of insects, chemical treatment was 17, and control (no treatment) was 32 families. B. thuringiensis-based bioinsecticides may be used in the intercropping system since it causes the highest biodiversity (H '= 2.96), high predator population (45%) and low pest population (41%) compared to other treatments.

Developmental the pattern of embryogenic microspore of rice (Oryza sativa L.) based on morphological characteristic Siti Nurbaiti; Yekti Asih Purwestri; Budi Setiadi Daryono; Endang Semiarti; Ari Indrianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 1 (2019): December 2019
Publisher : The East Java Biological Society

Microspore culture is a rapid approach to get double haploid plants in shorten time which is important for plant breeding. Microspore culture of pigmented rice cultivar Segreng was used to observe the pattern of embryogenic development based on the characteristics of morphology. Characterization between embryogenic and non-embryogenic microspores was conducted after exposed anther to carbohydrate starvation in B medium culture at 33oC for 4 days. The result showed that enlarged microspore and star-like structure of fragmented cytoplasm become a marker of embryogenic microspore after stresses treatment. More complex of fragmented microspore was found at 4 days in A2 medium and developed further into multicellular structure. These multicellular structures might be originated either by symmetrical or asymmetrical division. The longer incubation, the higher frequency of complex fragmented microspore that developed into multicellular structure release from exine has appeared.

Phytochemical properties and antioxidant activities of the leaf extracts of Boenninghausenia albiflora (Hook.) Rchb. ex. Meisn. (Rutaceae) I Putu Agus Hendra Wibawa; Putri Sri Andila; Vienna Saraswaty; I Gede Tirta
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Boenninghausenia albiflora is a well-known plant for being used in various traditional treatments. The plant is also reported to contain an essential oil which has a distinctive and pungent aroma. The present study aimed at investigating extraction yields, phytochemical compounds and antioxidants, and potential uses of B. albiflora leaves extracted with acetone solvent. Bioactive compounds of the leaf extract of B. albiflora were identified by GC-MS analysis while the antioxidant assay was measured by DPPH method. The extract obtained from acetone contained 30 compounds including coumarin group, 2-Isoprenyl-2,3-dihydro-furo [3,2-G] chromen-7-one (22.89% w/v) as the highest percentage compound. Other coumarins such as suberosin and dehydrogeijerin were also identified in this study. The IC50 values of the leaves extract of B. albiflora was 194.3 ppm which was higher than propyl gallate. Thus indicating B. albiflora as potential sources of anti-cancer, anti-inflammatory and antioxidant.

Profile of microbial community of naturally fermented Sumbawa mare’s milk using next-generation sequencing Yoga Dwi Jatmiko; Irfan Mustafa; Tri Ardyati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

This study aimed to investigate the bacterial and fungal/yeast diversity in naturally fermented Sumbawa mare’s milk through a next-generation sequencing approach, and evaluate the quality of fermented mare’s milk based on the presence of pathogenic or undesirable microorganisms. Microbial density determined using plate count agar (total aerobic bacteria), de Man Rogosa Sharpe agar (Lactobacillus), M17 agar (Lactococcus) and yeast peptone dextrose agar supplemented with streptomycin 50 ppm (yeast). Nutritional content and acidity level of each fermented milk sample were also evaluated. Genomic DNA was extracted using FastDNA Spin (MPBIO). The total gDNA was further analyzed using illumina high-throughput sequencing (paired-end reads), and the sequence results were analysed using QIIME v.1.9.1 to generate diversity profiles. The difference in nutrient content of mare’s milk was thought to affect the density and diversity of microbes that were able to grow. Fermented mare’s milk samples from Sumbawa had the highest bacterial diversity compared to samples from Bima and Dompu. However, fermented mare’s milk from Dompu had the best quality which was indicated by the absence of bacteria that have the potential to be pathogenic or food spoilage, such as members of the Enterobacteriaceae family (Enterobacter, Klebsiella and Escherichia-Shigella) and Pseudomonas. Genus of Kazachstania and Kluyveromyces, as well as family Dipodascaceae were frequently observed fungi/yeast from Sumbawa fermented mare’s milk. The presence of potential pathogenic bacteria warrants special attention in improving the hygiene of manufacturing process.

Seed storage importance and behaviour of a vulnerable species Angsana (Pterocarpus indicus Willd.) Ridesti Rindyastuti; Febrina Artauli Siahaan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

Angsana (Pterocarpus indicus Willd.) is one of woody plant species listed in the IUCN red list for priority in conservation as a vulnerable species. The effective and low-cost methods of seed storage is required to support the ex-situ conservation through seedling recruitments for population improvement. This study used completely randomized factorial design with 2 factors i.e. seed storage methods (room temperature, sealed jar and cold temperature), and storage duration (1,2,3,4 and 5 months). This study used 3 replications for each treatments and 10 seeds for each replication. The result showed that the seed of P. indicus start to germinate in 5-11 days after seeding while leaf emerged in 15-13 days after seeding. Seed viabilities generally were high until 3 months of storage (up to 65%) and start to decline at 4 months of storage at 51%. Storage methods and storage duration significantly influenced the seedling growth, viability, and Relative Growth Rate (RGR). There was an interaction between two factors. The storage at room temperature during a month (1RT) has the highest seedling height. The seeds stored in sealed jar during 5 months (5SJ) showed the highest seedling RGR, while the seeds stored in sealed jar during 3 months (3SJ) has the highest seed viability. For longer term storage, the seed of P. indicus should be stored in sealed jar or at room temperature at 11% moisture content. Both in the rainy and dry season, seedling recruitments were low and sapling stage was rare which showed low seedling establishment. Moreover, based on the phenological information, it is better to store the seed during the rainy season.

Effect of NPK and KNO3 fertilizer on yield and quality of Madura tobacco Prancak N1 Djajadi Djajadi; Roni Syaputra
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

Madura tobacco Prancak N1 is a new superior variety with higher quality but lower nicotine content than Prancak 95 tobacco variety. The rate and form of fertilizer for the new variety has not yet been defined so that farmers have to use adding nitrogen fertilizer their tobacco with no phosphorus and potassium fertilizer. The objective of the research was to determine effect of NPK and KNO3 fertilizers on yield and quality of Madura tobacco var. Prancak N1. The research was carried out at Cen-lecen village, Pakong Sub District of Pamekasan District, Madura. The experiment was laid out in a randomized block design with three replication of each plot. Treatments consisted of 9 packs of fertilization with NPK compound fertilizer (8-15-19), and KNO3 (13-0-45) and those were compared with 1 packed of recommended using single fertilizer of 40 kg N-ZA + 36 kg P2O5-SP36 + 25 kg K2O-ZK + 5 ton organic manure per hectare. Results showed that addition of compound fertilizers NPK plus KNO3 increased absorption of macro nutrients NPK, yield and quality of madura tobacco Prancak N1 variety. Package fertilizers NPK plus KNO3 with the rate of 40 kg N + 36 kg P2O5 + 61 K2O per hectare was suggested for madura tobacco Prancak N1 variety to produce high yield (846 kg/ha), grade index value (87.4), and crop index value (73.9).

In silico exploration the phenolic compound of olive leaves as acetylcholinesterase enzyme (AChE) inhibitor for Alzheimer’s disease therapy Nurul Jadid Mubarakati; Oktavia Rahayu Puspitarini; Tintrim Rahayu; Alik Maulidiyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

Olive (Olea europaea) have been cultivated and grown well in tropical climates such as Indonesia. Indonesia local community have used olive as herbal medicines due to its active compounds known as oleuropein that has many biological activities including as neurotherapy in Alzheimer's disease. The purpose of this study is to explore the potential phenolic compounds of olive and examine the acetylcholinesterase (AChE) inhibitory activity displayed by different olive polyphenols through a silico approach. The bioactive compounds of olive which had been analyzed in this study were phenolic compound included oleuropein, demethyl-oleuropein, ligstroside, oleoside, verbascoside, luteolin 7-glucoside, and hydroxytyrosol. Interaction of bioactive compounds with acetylcholinesterase (AChE) was analyzed through molecular specific docking using AutoDock Vina with Pyrx Software. The result elucidate that olive contain potential biological activities as antioxidant, anti-inflammatory, antineoplastic, free radical scavenger, antibacterial, antifungal, expression TP53 enhancer, caspase 8 stimulant, platelet adhesion inhibitor, treatment for lipoprotein disorder, antiviral and dementia treatment/Alzheimer disease. The highest bioactivity percentage of olive phenolic compound are as an antioxidant of 82%, anti-inflammatory of 73%, and anti-cancer (antineoplastic) of 70% respectively. Based on molecular docking analysis show one of olive phenolic compound of the dimethyl-oleuropein has strong interaction with AChE as pointed in the binding affinity of demethyl-oleuropein +AChE of -8.9 kcal/mol has closed to galanthamine binding affinity of -10.3 kcal/mol. Along with glutamate acid 202, tyrosine 133 and tyrosine 124 are the major contributors in the target-ligand interactions. The selected demethyl-oleuropein ought to be tested in clinical studies to discover new neuro-therapeutic candidates.

Effect of bait density on consumption rates and mortality of subterranean termite Coptotermes curvignathus Yuliati Indrayani; Dewi Fatmawati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

Utilization of baits for controlling termites is achieving high attention in recent year. One of affecting factors of baiting preference of termites including the density of bait. In this study, the effect of bait density on consumption rates and mortality of subterranean termite Coptotermes curvignathus was investigated. Baits prepared by 1:1:1 weight ratio of mixed blended official waste paper, cardboards and cajuput (Melaleuca leucadendra) leaf which were formed into a cube measuring 2x2x1 cm. The baits density were 0.5, 0.6, 0.7, 0.75, 0.8, 1.0 gr/cm3. No choice and multiple choice tests were conducted with five replications. Clear plastics cup measuring diameter 6 cm and 12 cm were employed for no choice test and multiple choice tests respectively. Fifty workers and five soldiers of C. curvignathus were used for no choice test, while 150 workers and 15 soldiers were tested in the multiple choices test. The results indicated that bait with lower density had a better consumption rates to C. curvignathus. The influence of bait density on termite mortality was also reported, since there is a relationship between consumption rates and termite mortality which is contrary to the general toxicity test. The percentage of consumption rates was still lower therefore further research is indispensible on reformulation of the baits.

Biofilm formation and detection of A/D genes in MRSA (Methicillin-Resistant Staphylococcus aureus) and MSSA (Methicillin-Sensitive Staphylococcus aureus) Rini Purbowati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

MRSA (Methicillin-Resistant Staphylococcus aureus) arises due to excessive use of methicillin antibiotics and complications from all medical practices that have been carried out lately. The prevalence of MRSA tends to increase and its ability to form biofilms so that it has the potential to significantly increase mortality and morbidity. Biofilms are considered one of the microbial virulence factors. Biofilm formation in S. aureus is regulated by the expression of PIA which mediates attachment between cells to cells and is a product of the ABDC ica gene. This study aims to determine the ability of biofilm formation as one of the virulence factors in MRSA compared to MSSA (Methicillin-Sensitive Staphylococcus aureus) . its relationship to the presence of A and D ica genes as biofilm coding genes. Biofilm formation assay on MRSA and MSSA using the Microtiter Plate (MtP) method and the A/D gene detection assay performed by PCR method. The results showed that the incubation end value of Optical Density (OD) for MSSA1 (0.75), MSSA2 (0.46), MRSA1 (0.53) isolates according to the formula were interpreted as moderate biofilm producer. The conclusions of this study were all of the test isolates (MSSA1, MSSA2 and MRSA1) were able to form a biofilm. Distribution of ica A and ica D genes in MSSA 1 and MSSA 2 was varied while MRSA1 had the ica A and ica D genes.

An Overview of Environmental Education for Millennial Visitors in Indonesian Botanic Gardens Rosniati A Risna; Anggun R Gumilang
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

In an attempt to meet the information and education needs of millennial visitors – the new tourism frontiers – in industrial revolution 4.0 era, environmental education program in Indonesian Botanic Gardens (IBGs) has applied a new paradigm. As the world become more digital, the IBGs has also developed more digital programs in terms of environment, biology and conservation education. There are mobile apps for self-guided tours, digital class for various topics related with horticulture practices, virtual adventurous games, online reservation, are to name some. Annual numbers of IBGs visitors showed increasing trends in period of study, which was probably caused by increasing quality of information, education program, digital facilities, and displays, including photo spots. This paper will describe how IBGs play important role in supporting the Sustainable Development Goals (SDGs) in quality education, as well as Target 14 of Global Strategy for Plant Conservation (GSPC) by making more engagement with millennial visitors.

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Sutiman Bambang Sumitro

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Sutiman Bambang Sumitro

Contact Email berkalahayati@yahoo.com

Phone +62341570631

Journal Mail Official wulidanisa@berkalahayati.org

Editorial Address Jalan Surakarta No. 5 Malang, Indonesia

Location Unknown, Unknown INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Sutiman Bambang Sumitro

Contact Email
berkalahayati@yahoo.com

Phone
+62341570631

Journal Mail Official
wulidanisa@berkalahayati.org

Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia

Location
Unknown,

Unknown

INDONESIA