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Contact Name
Sutiman Bambang Sumitro
Contact Email
berkalahayati@yahoo.com
Phone
+62341570631
Journal Mail Official
wulidanisa@berkalahayati.org
Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia
Location
Unknown,
Unknown
INDONESIA
Berkala Penelitian Hayati
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Articles 507 Documents
Virtual prediction of antiviral potential of ginger (Zingiber officinale) bioactive compounds against spike and MPro of SARS-CoV2 protein Ahmad Hafidul Ahkam; Feri Eko Hermanto; Adzral Alamsyah; Iva Himmatul Aliyyah; Fatchiyah Fatchiyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (33.627 KB) | DOI: 10.23869/50

Abstract

Coronavirus disease 2019 (COVID-19) is a human disease caused by SARS-CoV2 becomes a serious health threat after infected more than 6 million people globally. The virus enters the host cell through an S protein on its surface and begins its life cycle with the help of a key protein, MPro. On the other hand, several bioactive from Ginger have been reported for their antiviral properties, but few studies related to COVID-19. This study aims to evaluate the potential of a few bioactive compounds from Ginger as anti-SARS-CoV2. Gingerenone A, gingerol, geraniol, shogaol, zingiberene, zingiberenol, and zingerone were used as ligand to be docked with S protein and MPro. Drug-likeness properties also evaluated using SwissADME. Gingerenone A constantly gave the lowest binding energy compared to others both with S or MPro. However, gingerol, geraniol, shogaol, zingiberene, zingiberenol, and zingerone could interact with key residues responsible for MPro catalytic domain, while geraniol, shogaol, zingiberene, zingiberenol, and zingerone could interfere S-ACE2 binding shape and increase its binding energy. The drug-likeness analysis also revealed that all of the analyzed compounds have no violation of Lipinski’s Rule of 5. In conclusion, gingerol, geraniol, shogaol, zingiberene, zingiberenol, and zingerone from Ginger have good potential as antiviral agents with good oral bioavailability and flexibility
Effect of gibberellin to honeydew severity and agronomical characters of soybean genotypes attacked by Bemisia tabaci Dyah Kusuma Wardhani Syakhbikhisma Arsy; Siti Zubaidah; Heru Kuswantoro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 1 (2017): December 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.685 KB) | DOI: 10.23869/53

Abstract

One of the factors that reduces soybean production is pest attack such as Bemisia tabaci. To increase soybean production, the physiological processes of the plant have to grow well causing optimal plant growth. It can be optimized by adding gibberellin. The research aimed to study the influence of gibberellin concentrations to honeydew severity and agronomical characters of soybean genotypes attacked by Bemisia tabaci. The gibberellin was given when the soybean plant was 35 days after planting. The gibberellin concentrations were 0, 25, 50, and 75 ppm. The result showed that the difference gibberellin concentrations did not affect honeydew severity, but the genotypes affected honeydew severity. The most susceptible genotype was shown by Gumitir. In the agronomical characters, UM 4-1 had the highest number of filled pods, but it was not significantly different from the variety of Wilis and UM 2-4. It was closely related to the number of total pods in which UM 4-1 also became the genotype producing the greatest number of total pods, although the yield was not quite different from the variety of Wilis and UM 2-4. Genotype of UM 4-1 also showed the highest number of total reproductive nodes per plant. The seed thickness was also influenced by genotype × gibberellins interaction. UM 2-4 at 50 ppm resulted the thickest seed. The 50 ppm concentration can provide an impact with the greatest number of total reproductive nodes per plant than the other concentrations. UM 2-4 also showed the highest number of total pod at 75 ppm concentration. The 50 ppm concentration is recommended to be given since it can influence the number of filled pod, number of total pods, seed thickness and number of total reproductive nodes per plant. The gibberellin concentration significantly influenced the number of filled pods, number of total pods, and number of total reproductive nodes per plant.
The effect of lactic acid fermentation on fig (Ficus carica) fruit flavonoid Ernanin Dyah Wijayanti; Nur Candra Eka Setiawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 1 (2017): December 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (543.503 KB) | DOI: 10.23869/54

Abstract

Fig fruit contains a quite high flavonoid that supports the use of it for several disease therapies. Yet, most of the flavonoid in plants is difficult to be digested since it bounds with the glycoside, so the hydrolysis is necessary. The hydrolysis can be done through the lactic acid fermentation. This research aims to determine the effect of lactic acid fermentation on fig fruit flavonoid. Dried fig fruit was prepared into a fig fruit extract and fermented at 37°C for 24 hours using 4 types of starter bacteria; Lactobacillus acidophilus, L. bulgaricus, L. casei and L. plantarum. The fermentation result was identified its flavonoid using dye reaction, determined its flavonoid content using the spectrophotometry with the quercetin standard, and analyzed qualitatively using the LC-MS/MS. The results show that the flavonoid was identified in both before and after the fermentation, but the flavonoid content decreases 30 – 50% after the fermentation. The LC-MS/MS shows that the identified flavonoid is rutin, with the relatively higher percentage after the fermentation. In addition, the catechin and epicatechin are not detected. It can be concluded that the lactic acid fermentation affects the fig fruit flavonoid. The fermentation with all types of starter bacteria decreases the total flavonoid content of fig fruit juice.
Diversity of parasitic plants and their hosts in Kepala Jeri and Pemping agroforestry Batam Indonesia Solikin Solikin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 1 (2017): December 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (702.885 KB) | DOI: 10.23869/55

Abstract

Parasitic plants lived by adhering, absorbing water, minerals and food to host plants. The objective of this research was to determine the diversity of the parasitic plants and their hosts in agroforestry lands where farmers cultivated various plants around their houses or forests. The research was conducted in Kepala Jeri and Pemping Islands, Batam, Riau Islands Province in October 2014 using exploration methods. Plant inventory and data collection were done along determined exploration tracks. There were three species of the parasitic plants were found namely Cassytha filiformis L., Dendrophthoe pauciflora Dans. and Viscum stenocarpum Dans. The most dominant parasitic plant was C. filiformis, whereas the least dominant parasitic plant was V. stenocarpum. There were 26 species, from 24 genera and 17 families of host plants of C. filiformis whereas hosts of D. pauciflora were 15 species, from 12 genera and 9 families.
The potency of Micrococcus sp. L II 61 bacteria as oil sludge cleaning agent Ni’matuzahroh Ni’matuzahroh; Intan Ayu Pratiwi; Tini Surtiningsih; Fatimah Fatimah; Sri Sumarsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.394 KB) | DOI: 10.23869/56

Abstract

This research aimed to reveal the ability of Micrococcus sp. L II 61 bacteria that was isolated from Pegirian Surabaya as oil cleaning agent. This is an experimental research to detect the presence of biosurfactant and lipase enzyme in culture supernatant of Micrococcus sp. L II 61 with aliphatic hy-drocarbon (cooking oil) as a substrate growth. Biosurfactant production was evaluated by measuring the surface tension of supernatant using tensiom-eter du Nouy and measuring the emulsification activity value using diesel oil as hydrocarbon test. Lipase enzyme was detected by measuring lipolitic activity value of crude enzyme (culture supernatant) by using p-nitrofenil palmitic (p-npp) as a substrate test. Data were analyzed descriptively. The results showed that Micrococcus sp. L II 61 produced biosurfactant with surface tension decreasing of culture supernatant up to 30.27 ± 1.17 mN/m compared than aquadest and value of hydrocarbon emulsification activity (AE 1 hour) up to 20.24 ± 0.68 %. Culture of Micrococcus sp. L II 61 after 16 hours incubation have a lipolytic activity 33.53 ± 0.14 U/mL at pH 7 and 37 oC. Supernatant of Micrococcus sp. L II 61 100% (v/v) give the high-est percentage of oil sludge solubility, i.e. 86.38 ± 2.39%. Micrococcus sp. L II 61 is a highly potential to be developed as oil sludge cleaning agent.
Molecular biology education in Indonesia - suggestions for improvement Wolfgang Nellen; Atika Hanoum Rahasta; Feri Eko Hermanto; Margareth Rika Wahyusari Gabriella Lorenza
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (825.118 KB) | DOI: 10.23869/59

Abstract

Education in molecular biology is considered difficult because it is complex and requires a lot of basic knowledge. Focused on basic science facts, it is usually taught with little connection to everyday life. A second problem is that the topic is rather abstract and results are only indirectly observed: we know that DNA is a double helix - but nobody has seen it directly. In contrast to subjects in the humanities and social sciences, molecular biology is a handy-craft where sophisticated theory and practical lab-work go hand in hand. Molecular biology is not so much a specific sub-discipline of biosciences but rather a huge tool box which is indispensable for all other fields of biology. It is therefore absolutely required for all biologists to know the basics and the potential of molecular biology in their specific field of interest. We present some thoughts how to make the theory more attractive, how to combine learning, training and teaching of theory and practice, how to stimulate independent, critical thinking in students and final-ly, how to identify and support excellent students who should advance science in the next generation. Last not least, we emphasize the necessity to expand information and teaching of molecular biology to the public.
Potential probiotic evaluation of two Lactobacillus plantarum strains isolated from Indonesian fermented food and fruit A’liyatur Rosyidah; Heddy Julistiono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (707.508 KB) | DOI: 10.23869/60

Abstract

Two Lactobacillus plantarum strains, InaCC B122 and InaCC B128, isolated from Indonesian fermented food, “dadih” and fruit were evaluated for probiotic potential. These two lactobacilli strains were subjected to a series of in vitro test to assess their surviving capability in gastrointestinal track and antimicrobial activity. The test included acid and bile salt tolerance, adhesion to the yeast Saccharomyces cereviseae cells, heat resistance and antimicrobial activity against Mycobacterium smegmatis, Escherichia coli, and Staphylococcus aureus. Both strains were able to grow under acid and bile salt condition for 24 h. Co-aggregation percentage of strain InaCC B122 and InaCC B128 against the yeast cells was 52.3 % and 53.8 % respec-tively. Heat treatments (45 ℃ for 30 min. and 55 ℃ for 15 min.) did not affect co-aggregation percentages. Their supernatants had antimicrobial activity against M. smegmatis, E. coli and S. aureus with MIC of 5 %, 10 % and 10 % respectively. In conclusion, based on this assessment, these strains are good candidate for probiotic.
Quercetin, rutin, proanthocyanidin, catechin and epitacethin as fatty acid synthase inhibitor using virtual screening Candra Rini Hasanah Putri; Sutiman Bambang Sumitro; Setyawati Karyono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 1 (2017): December 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.501 KB) | DOI: 10.23869/62

Abstract

Fatty acid synthase is known to participate in the occurrence of malignancies, so fatty acid synthase inhibition is expected can restrain malignancy. In this research, virtual screening is done by molecular docking between the active ingredients in Tamarindus indica with three domains of FAS. In thioesterase domain, it turns out all of the active ingredients in Tamarindus indica can bind thioesterase domain right in the place where orlistat (as a reference inhibitor) bonded, with a higher strength than the orlistat. In enoyl [acyl-carrier-protein] reductase domain, it turns out the binding affinity of quercetin, rutin, catechin and epicatechin against fatty acid synthase are greater than the reference inhibitor, triclosan. In malonyl-CoA / acetyl-CoA-ACP-transacylase domain, it turns out the binding affinity of quercetin, rutine, proanthocyanidin, and catechin against fatty acid synthase is greater than the natural substrate, Malonyl-CoA. The high binding affinity of the active ingredients in Tamarindus indica against the two domains of fatty acid synthase that may also can be occupied by reference inhibitors, showed the ability of Tamarindus indica as an inhibitor of fatty acid synthase. While the high ability of active ingredients in Tamarindus indica to bind to a domain that should be occupied by the natural substrate of fatty acid synthase (malonyl-CoA) demonstrated the ability of Tamarindus indica to inhibit fatty acid synthase's work in a way to compete with the natural substrate. This study shows that Tamarindus indica may serve as anti-malignancy through its ability to inhibit fatty acid synthase.
Metallothionein analysis and cell damage levels on the liver and gill Of Barbonymus gonionotus In Brantas River, Indonesia Alfiah Hayati; Nita Yuliarini; Agoes Soegianto; Hana Widyana; Inesavira Rindaputri; Nuris Auliya; Putri Ayu Ika
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 1 (2017): December 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (446.438 KB) | DOI: 10.23869/63

Abstract

This study aimed to analyze the levels of metallothionein and damage to hepatocytes and gills of Barbonymus gonionotus in the Brantas River (upstream and downstream), and the correlation levels of metallothionein in the gills and liver of Barbonymus gonionotus with different sampling time variation (March, June, and September 2016). Fishes were caught using trawl in two stations (Karangkates Reservoir and the Kali Jagir river). Fish gills and livers were taken for histopathological analysis and levels of metallothionein were measured by ELISA method. Gills histopathological analysis showed that the highest damage in Karangkates reservoir dan Kali Jagir river is hyperplasia. Liver histopathological analysis in Karangkates reservoir showed normal hepatocytes meanwhile necrosis is highest damage found in Kali Jagir river. Metallothionein levels of fish in Karangkates reservoir for March, June, and September were 0.18 ng/mL; 0,18 ng/mL; and 0.2 ng/mL (gills fish); 0,18 ng/mL; 0,34 ng/mL; and 0,21 ng/mL (liver fish) respectively. Metallothionein levels of fish in Kali Jagir river for March, June, and September were 0,26 ng/mL; 0,18 ng/mL; and 0,18 ng/mL (gills fish); 0,2 ng/mL; 0,45 ng/mL; 0,19 ng/mL (liver fish) respectively. We conclude that the damage to the gills and liver of Barbonymus gonionotus was mostly found in Kali Jagir River and the highest metallothionein level of Barbonymus gonionotus also presented in Kali Jagir River.
The utilization of ink cap mushroom (Coprinus cinereus) on palm oil mill effluent degradation Yohanes Bernard Subowo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.433 KB) | DOI: 10.23869/64

Abstract

A research on the utilization of ink cap mushroom (Coprinus cinereus) on Palm Oil Mill Effluent (POME) degradation was conducted. Palm oil in-dustry is an agricultural industry which keeps on developing in Indonesia. Aside from producing palm oil, this industry also generates POME. POME has high level of BOD and COD value thus it is dangerous for the environment. Coprinus cinereus was examined to degrade POME because of its ability to produce ligninases enzymes. The aim of this research was to evaluate ligninases enzymes produced by C. cinereus after the addition of inducers and its ability in degrading POME. Coprinus cinereus was cultivated on Defined Medium (DM) then the mycelia was harvested Inducers used in this study were CuSO4, KNO3 and sucrose. The suspension of Coprinus cinereus mycelia was used to degrade POME. The result showed that Coprinus cinereus could produce Laccase, Mangan Peroxidase and Lignin Peroxidase on DM. The addition of 200 μM CuSO4 and 10 mM KNO3 on DM could increase laccase activity by 62.92 %. Coprinus cinereus has ability to degrade Poly R-478 and POME. The treatment of 200 μM CuSO4 on media could increase the degradation of Poly R-478 by 19.32% after 9 days of incubation. The addition of 15 g/L sucrose on POME could increase decolorization by 75.26 % and COD reduction by 91.26 % after 27 days of incubation. Ink Cap Mushroom (Coprinus cinereus) can be used for POME degradation. The addition of sucrose and heating treatment would accelerate COD reduction.

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