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Jurnal Natur Indonesia

JN
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Published by Universitas Riau

ISSN : 14109379 EISSN : 25030345 DOI : http://dx.doi.org/10.31258/jnat

Core Subject : Science, Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

JURNAL NATUR INDONESIA terbit sejak tahun 1998, merupakan jurnal ilmu sains yang menyajikan artikel mengenai hasil penelitian, pemikiran dan pandangan dari peneliti dan pakar dalam bidang biosains (ilmu dasar), meliputi biologi, fisika, kimia dan matematika. Jurnal Natur Indonesia melibatkan mitra bestari yang menelaah setiap artikel sesuai dengan bidang ilmunya. Nama dan asal institusi mitra bestari tersebut tercantum pada halaman bagian normor 2 dari setiap volume penerbitan. Jurnal ini diterbitkan setahun dua kali, pada bulan April dan Oktober.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 10 Documents

Search results for , issue "Vol 10, No 2 (2008)" : 10 Documents clear

Pengaruh Spesies Inang dan Sumber Nutrisi Terhadap Produksi Spora Fungi Mikoriza Arbuskula Delvian, Delvian
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

An experiment to study the effect of host species and source of nutrient on spore production of an ArbusculeMycorrhizal Fungus, Gigaspora margarita, has been carried out. Combination treatments studied consisted ofhost species (Zea mays and Pueraria javanica) and nutrient factor (no fertilizer, fertilizer and Red Hyponex).Growth medium used was a mix of soil and sand in 1: 1 ratio (v/v) which was sterilized. Results of the experimentshowed that spore production of G. margarita was affected by host species and also by nutrient applied, thoughthere was no interaction between the two factors. However, P. javanica produced more spore than Z. mays.Treatment with Red Hyponex result much higher spores than others, but however without fertilizer produced morespores than Urea, TSP and KCl fertilizers.

Uji parasitik Beberapa Spesies Jamur Tanah terhadap Globodera rostochiensis (Woll.) Secara In Vitro Kalay, A. Marthin; Natasasmita, S; Suganda, T; Simarmata, T
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Potato cyst nematode (Globodera rostochiensis Woll.) is an important plant pathogen on potatoes. The development of this nematode in soil could be controlled by using soil fungi. In vitro assay to determine the capacity of soil fungi Fusarium oxysporum TR1, F. solani TR2, F. oxysporum KT1, F. chlamydosporum KT2, F. oxysporum SM1, Paecilomyces lilacinus SM3, and F. chlamydosporum SM4 to parasite G. rostochiensis has been cariied out. The results showed that all of tested fungal species enable to reduce the number of living J2 out from the cyst. The highest J2 reduction, 67.48%, was evidence by using P. lilacinus.

Isolasi dan Karakterisasi Protease Ekstraseluler dari Bakteri dalam Limbah Cair Tahu Fatoni, Amin; Zusfahair, Zusfahair; Lestari, Puji
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Protease has been used in large application industrial process such as detergent, leather, textil, softdrink, andmedicine. In order to find unique protease, many substances were explored as proteases of bacteria sources. Inthis study, tofu liquid waste was used as a source of bacteria producing proteases. Waste sample was growth inskim milk agar medium showing proteases activity, it was used to produce extracellular protease. The microbialcolonies were identified as Staphyllococcus sp. Protease was extracted with 5000 g centrifugation at 4 0C, andpurificated with ammonium sulphate precipitation continued with dialisis. Optimum production time, pH, metal ion,EDTA, specific activity, KM, and Vmaks were studied for enzyme characterization. Volume of crude enzyme was 300ml, with spesific activity of 3.55 U/mg. Protease obtained from 60% ammonium sulphate fraction had the highestspecific activity of 68.22 U/mg. Study on the protease characterization revealed that optimum temperature of thisenzyme was 400C. The optimum pH of the enzyme was found to be 8.0. The kinetic parameters K M dan Vmaks withcasein as substrate were 0.31% and 51.55 U/ml. Some inhibitory effect was observed in the presence of EDTA, Cu +2,Co+2, Zn+2, and enzyme activity was stimulated by Mg+2, indicating that this ion had a functional role in the molecularstructure of the enzyme.

Asimtotik Model Multivariate Adaptive Regression Spline Otok, Bambang Widjanarko; Guritno, Suryo; Subanar, Subanar
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Parameter estimation in MARS model executed by minimizing penalized least-squarer (PLS). Through somerequirement, asymtotic estimator characteristic from MARS prediction model has been successfully proven. Theresearch result shows that GCV can work properly to determine the best model that applied on MARS model. Solar’s vehicles produce opacity that exceed the standard limit of emition quality which was adjusted in Kepmen LH No.35 Year 1993, as large as 88 percent from 408 percent. Applying years, cylinder volume, type of machine, andvehicle’s radius are the variables that influences the opacity.

Kopolimerisasi Grafting Campuran N-Isopropilakrilamida dan Glisidil Metakrilat pada Film Polietilen yang Diinduksi oleh Sinar Ultraviolet Suka, Irwan Ginting
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Liquid phase ultraviolet irradiation was used to graft the mixtures of N-isopropylacrylamide and glycidyl methacrylateto a linear low-density polyethylene film (thickness of 30 μm) surface. Xanthone was used as photoinitiator, whichwas coated on the film surface earlier. The surface of the grafted polyethylene samples were characterized byattenuated total-reflection IR spectroscopy and scanning electron microscopy. Elemental analysis indicated thatthe NIPAAm fraction with respect to GMA in the graft copolymer increased with increasing NIPAAm ratio in thecomonomer feed solution. The reactivity ratios of NIPAAm and GMA monomers determined in the present graftcopolymerization system were found to be 0,31 ± 0,1 and 4,8 ± 0,2, respectively. It was found that the epoxy groupsof GMA-grafted chains in the NIPAAm/GMA-grafted films have the ability to react with ethylenediamine (En). Theaminated NIPAAm/GMA-grafted film had an excellent ability to adsorb cupric ion. The temperature-responsivecharacter of the resulting grafted films was evaluated by measuring the water absorbency of the grafted NIPAAm/GMA when immersed in water and/or methanol at 5 to 50 0C.

Analisis Komposisi dan Kandungan Karotenoid Total dan Vitamin A Fraksi Cair dan Padat Minyak Sawit Kasar (CPO) Menggunakan KCKT Detektor PDA Syahputra, M. Rio; Karwur, Ferry F; Limantara, Leenawaty
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

This study was carried out on two phases of Crude Palm Oil (CPO) to determine the total and composition ofcarotenoid and vitamin A content. Total of carotenoid was analyzed using spectrophotometer UV-Vis, and then theresult was calculated by Gross (1991) equation. The vitamin A content was calculated by NAS-NRC equation (1974).The type and composition of both phases of CPO were determined by Choo’s method (1994) by using HPLC withPhoto Diode Array (PDA) detector. The sample was prepared in two methods, with and without saponification. Theresult shows that total carotenoids in liquid and solid phase of CPO are 536 ± 13.2 g/g (liquid), 352 ± 17.7 μg/g(solid) and the vitamin A were 89.4 ± 2.2 RE (liquid), 58.7 ± 3.0 RE (solid), respectively. The carotenoid compositionsof both phases of CPO were dominated by - and -carotenes. The result shows that - and -carotenes preparedby saponification method in liquid phase are 29.03% and 60.88%, and without saponification (direct method) are28.14% and 59.44%. The result for solid phase shows that - and -carotenes by saponification are 25.89% and60.81%, and without saponification (direct method) are 30.00% and 56.92%. The research also shows the advantagesof using HPLC with PDA detector for identification and analysis of type and carotenoid composition.

Urgensi dan Mekanisme Biosintesis Metabolit Sekunder Mikroba Laut Nofiani, Risa
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Marine microorganism is one of biologically active potential resources of secondary metabolites. Its potency areso promising that the knowledge of how its secondary metabolite occured need to be studied and collected. Thoseknowledges will enable further study is improving secondary metabolite production in the laboratory. In nature,secondary metabolites synthesis occur when there are effect of both biotic and abiotic factors such as sea waterand microbe symbiosis with other living materials. When this is explained in metabolic pathways, secondarymetabolite synthesis affected by available nutrient and regulated by autoinducer molecules through quorum sensingmechanism

Penapisan Aktivitas dan Senyawa Antidiabetes Ekstrak Air Daun Dandang Gendis (Clinacanthus nutans) Nurulita, Yuana; Dhanutirto, Haryanto; Soemardji, Andreanus A.
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

An investigation of the antidiabetic activity of Aqueous Extract and its fractions from a different polarity solvent:ethanol, ethyl acetate, and n-hexane of Dandang Gendis Leaves (Clinacanthus nutans Lindau) on Mile SwissWebster Mice has been carried out. The effect of aqueous extract of dose of 50, 100, and 150 mg/kg BW wastested on glucose serum level of alloxan induced that mice. Its fractions were tested by glucose tolerance method.Aqueous extract (150 mg/kg BW) significantly decreased blood glucose serum level (pd”0,05) that had the sameeffect as oral glibenclamide (0,65 mg/kg BW) administrated once a day for 9 days. Aqueous extract that wasfractionated has 5 fraction. Fraction of ethanol extract that was insoluble in ethyl acetate at a dose of 100 mg/kgBW was the most active fraction, that significantly (p<0,05) decreased blood glucose serum level of mice atglucose tolerance method of 18,4% in 3 hours after 2 g/kg BW of glucose administration. Thin layer chromatography(TLC) of that fraction ethanol 95%-chloroform 9:2 as eluent gave 5 spots under ultraviolet ray and showed spesificcompounds by qualitative test. Spot 1 (steroid) gave Rf 0,14; spot 2 and spot 3 that gave Rf 0,53 and 0, 67 wereflavonoid, spot 4 (triterpenoid) gave Rf 0,77; and spot 5 (chlorophyll) gave Rf 0,89. This research show that this plantcan used for hipoglicemic traditional medicine.

Microbiological and Ecophysiological Characterization of Green Algae Dunaliella sp. for Improvement of Carotenoid Production ainuri, Muhammad Z; Kusumaningrum, Hermin P.; Kusdiyantini, Endang
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

An isolate of green algae Dunaliella sp. from BBAP Jepara is usually used as a source for carotenoid supplementfor marine animal cultivation in the local area. In order to improve carotenoid production especially detection ofbiosynthetic pathway from the organisms investigated in this study, the main purpose of this study is characterizingDunaliella sp. based on it’s microbiological and ecophysiological characters. The research was done by characterizethe growth, the cell and colonies microbiologically, total pigment production, and also characterize all of theecophysiological factors affecting the algal growth and survival. The results of this research showed that Dunaliellasp. posseses typical characteristic of green eucaryote alga, in their growth and ecological condition. The extremecharacters which was toleration ability to high salinity environment of was used to conclude Dunaliella sp. asDunaliella salina.

Teknologi Fragmentasi Buatan Karang (Caulastrea furcata dan Cynarina lacrimalis) dalam Upaya Percepatan Pertumbuhan pada Kondisi Terkontrol Zulfikar, Zulfikar; Soedharma, Dedi
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The objective of research were analyze water quality condition of water circulation system at laboratory and tomeasured growth survival rate of Caulastrea furcata and Cynarina lacrimalis which was fragmented at laboratory.Fragmentation treatment of Caulastrea furcata become 1, 2, 3, and 4 polyp that was rearing on circulation systemdid not give significant impact on height and length growth after 160 days rearing and fragmentation of Cynarinalacrimalis on circulation system give significant impact. Mean of growth length of Caulastrea furcata on treatment1, 2, 3 and 4 polyp in every month after 160 days was 1.64 mm, 1.55 mm, 1.42 mm , and1.08 mm whereas growthbroad was 0.71 mm, 0.82 mm, 0.51 mm, 0.62 mm, and mean of growth length Cynarina lacrimalis for the sametreatment in every month was 1.47 mm, 0.90 mm, 0.62 mm, 0.61 mm whereas growth broad was 1.57 mm, 1.16mm, 0.93 mm, 0.89 mm. Fragmentation treatment of Caulastrea furcata become 1 polyp was best length if compareother treatment and Cynarina lacrimalis was treatment became 2 devide.

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Titania T Nugroho

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Titania T Nugroho

Contact Email titania.nugroho@lecturer.unri.ac.id

Phone +62811764031

Journal Mail Official natur@ejournal.unri.ac.id

Editorial Address Gedung LPPM Universitas Riau Jl. HR Subrantas Km 12,5 Pekanbaru 28293

Location Kota pekanbaru, Riau INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Titania T Nugroho

Contact Email
titania.nugroho@lecturer.unri.ac.id

Phone
+62811764031

Journal Mail Official
natur@ejournal.unri.ac.id

Editorial Address
Gedung LPPM Universitas Riau Jl. HR Subrantas Km 12,5 Pekanbaru 28293

Location
Kota pekanbaru,

Riau

INDONESIA