Jurnal Natur Indonesia
JURNAL NATUR INDONESIA terbit sejak tahun 1998, merupakan jurnal ilmu sains yang menyajikan artikel mengenai hasil penelitian, pemikiran dan pandangan dari peneliti dan pakar dalam bidang biosains (ilmu dasar), meliputi biologi, fisika, kimia dan matematika. Jurnal Natur Indonesia melibatkan mitra bestari yang menelaah setiap artikel sesuai dengan bidang ilmunya. Nama dan asal institusi mitra bestari tersebut tercantum pada halaman bagian normor 2 dari setiap volume penerbitan. Jurnal ini diterbitkan setahun dua kali, pada bulan April dan Oktober.
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Aktivitas Promoter â-aktin Ikan Medaka Jepang (Oryzias latipes) pada Ikan Mas (Cyprinus carpio)
', Alimuddin;
Purwanti, Lola Irma;
Ath-thar, MH. Fariduddin;
Muluk, Chairul;
Carman, Odang;
Sumantadinata, Komar
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau
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DOI: 10.31258/jnat.11.2.70-77
This study was conducted to examine activity of medaka (Oryzias latipes) â-actin promoter (mBP) in common carp(Cyprinus carpio) as the first step towards development of common carp transgenic in country. Gene constructpmBP-hrGFP that consists of mBA promoter and humanized Renilla reniformis green fluorescent protein gene(hrGFP) was injected into cytoplasm of one cell stage of common carp by using microinjector. PmBP-hrGFPconcentration used for microinjection was 50 μg/mL aquabides. Parameters observed were survival rate of embryo(SRe), hatching rate (HR) and expression of hrGFP gene. SRe was calculated before eggs hacthed, while hatchingrate (HR) was after all of eggs hatched. The activity of mBA promoter was analyzed by observation of hrGFP genetransient expression using a fluorescence microscope. The results of experiment showed that SRe (87,5%) andHR (79.2%) of control was respectevily higher than that of injected treatment (75.0% & 61.7%). Expression of hrGFPwas observed firstly at blastula (12 hours after fertilization) to 1-day-old larval stages (24 hours after hatching)with higher gene expression at blastula to late gastrula stages. Percentage of micronjected larvae expressinghrGFP at 6 hours after hatching reached 71.6 ± 6.7%. Conclusion was that mBA promoter could drove hrGFPexpression in common carp, hence it can be used to produce common carp transgenic by changing hrGFP withgenes correlated with important traits in aquaculture.
Faktor-Faktor yang Mempengaruhi Produksi Biodiesel dari Minyak Sawit Mentah Menggunakan Katalis Padat Kalsium Karbonat yang Dipijarkan
Awaluddin, Amir;
', Saryono;
Nelvia, Sri;
', Wahyuni
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau
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DOI: 10.31258/jnat.11.2.129-134
The demand for petroleum has increased recently due to the increase of world population, industries andtransportation. Biodiesel (fatty acids methyl esters) has become attractive because of high price of petroleum,limited recourses of crude oil, and environmental concerns. Most biodiesel is produced by transesterification oftriglycerides of refined/edible type oils using methanol and homogeneous catalyst such NaOH and KOH. The useof heterogeneous calcined CaCO3 catalyst, has advantages such as the ease of phase separation betweencatalyst and biodiesel. This paper presents factors affecting the synthesis of biodiesel from crude palm using thecalcined CaCO3 catalyst . The synthesis is carried out by two steps, the acid-catalyzed pre-esterification of free-fatty acid and followed by base-catalyzed transesterification of triglycerides. A study of optimizing the reactioncondition of the esterification followed by transesterification of crude palm oil (CPO) is performed to obtainmaximum production of biodiesel. Under conditions of catalyst calcination temperature of 9000C, reactor time of1.5 hours, catalyst dosage of 1,5%, reaction temperature of 700C and methanol/oil molar ratio of 9 : 1, the oilconversion is 74,6%. The as-synthesized biodiesel meets the requirements of Indonesian National Standard (SNI)for biodiesel.
Kowanin, Suatu Santon dari Kulit Batang Garcinia cowa Roxb
', Darwati;
Bahti, Husen H;
', Supriyatna;
', Dachriyanus
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau
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DOI: 10.31258/jnat.11.2.109-114
The compound tetraoxygenated xanthone was isolated from the crude extract of the stem bark of Garcinia cowaRoxb. The compound tetraoxygenated xanthone was caried out as yellow crystal with melting point 136-1370C.The structure of this compound was detremined base on spectroscopic methods, including UV, IR, 1H-NMR,13C-NMR 1D and 2D. The compound was found to exhibit cytotoxicity against T47D cell by SRB method
Leaf Anatomy of Three Varians of Arundina graminifolia (D. Don.) Hochr
Sulistiarini, Diah;
Tihurua, Eka Fatmawati
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau
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DOI: 10.31258/jnat.11.2.78-82
Three variations of Arundina graminifolia flowers have been recognized, (1) group of big flower with reddish violetlip and purplelish white sepals and petals (2) group of small flower with white lip and white sepals and petals (3)similar to second variation but with purple color on the lip apex. In order to clarify the taxonomy status of thosethree variations, a leaf anatomical study had been carried out. Results of this study showed that there were somedifferences among them in trichome shape and number mesophyll layers. Big flower group of Arundina hastrichome club shape and thick mucous in the apex with mesophyll layer 9-11; for small flower group has white lip,trichome club shape, thick mucous and granulate in the apex, but only 2 mesophyl layers; whereas for other smallflower group with purple lip has trichome cup shape and 10-13 layers of mesophyll. Based on the differences inflower size and their color variation, and combined with leaf anatomical study, we suggested the three variationsof A. graminifolia should be in separated groups.
Biologi Perbungaan Keladi Tikus (Typhonium flageliforme): Seludang Bunga Menghambat Penyerbukan?
Rachman, Erlin
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau
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DOI: 10.31258/jnat.11.2.83-88
A study on the flower biology on keladi tikus (T. flageliforme) was conducted to uncover if its spathe inhibitspollination from external pollen resources and causes very low fruit set. The spathe box closely envelopes thesexually vital properties of its inflorenscence. Observation was carried out on some plant clump grown on someuniform potting. A hole was made with scalpel about 4 x 5 mm on a side of the spathe to break its isolation. Somenearer flowers in same clump were left without treatment at all as experimental control. Parameter observed waspresence or absence of seeded fruit setting on spadix. The study result showed that almost all treated flowerspadix per potting, set seeded fruits significantly higher (63, 69 ± 37, 64) than that of controls (3, 57 ± 9, 45) so it isa great possibility that spathe can inhibit partially fruit set. Flower structure and its reproductive phenology werealso described.
Bio-Oil dari Limbah Padat Sawit dengan Metoda Pirolisa
Saputra, Edy;
Bahri, Syaiful
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau
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DOI: 10.31258/jnat.11.2.124-128
Bio oil is most important alternative energy resources for human life due to the production of fossil fuel tend todecline. Numbers of researches have been developed on alternative energy resources, such as the conversion ofbiomass. The palm solid waste which is consisted of wood, stem and empty bunch having size of diameter in rangeof 2 to 10 mesh were used in this work. The pyrolysis method is selected in this research. This process was donein stainless steel reactor having length and length of 60.0 and 3.81 cm respectively at temperature of 450 to 600oCunder nitrogen flow. Gas chromatography HP 5890 is utilized to analyze of the bio oil result. The result achieved thehighest yield at 500oC and particle diameter of 2-6 mesh.
Keragaman Lumut pada Marga Pandanus di Taman Nasional Ujung Kulon, Banten
Windadri, Florentina Indah
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau
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DOI: 10.31258/jnat.11.2.89-93
Ujung Kulon National Park is one of conservation area in Banten Province. A research on bryophytes that grow onpandanus substrat in this area have been done at March to April 2008. The surrounding areas methode used onthis research. In the collections found 9 species usually growth on bark of Pandanus dubius. Two species wasreported as the important species, because one species (Fissidens teysmanianum Doz. & Molk) is endemicspecies in Java and the other (Calymperes cougiense Besch.) as abundant species in Malesia and it is only foundin Polynesia region.
Keanekaragaman Makrozoobenthos pada Hutan Mangrove yang Direhabilitasi di Pantai Timur Sumatera Utara
', Onrizal;
Simarmata, Fernades SP;
Wahyuningsih, Hesti
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau
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DOI: 10.31258/jnat.11.2.94-103
Macrozoobenthos is a group of important fauna in mangrove ecosystem. Mangrove rehabilitation is required toincrease biodiversity and density of fauna, including macrozoobenthos. The aim of this research was to studyinfluence of biotic and abiotic factors to diversity and abundance of macrozoobenthos. The research was conductedat natural and rehabilitated mangrove forest in Percut Sei Tuan, east coastal of North Sumatra. The results showedthat density of macrozoobenthos were significantly correlated to the soil texture, soil organic carbon and pH.Besides, macrozoobenthos diversity was mostly influenced by stand basal area, salinity and silt proportion of soiltexture.
Perubahan Bentuk Planlet Pisang Raja Sereh Hasil Mutasi dengan Ethyl Methane Sulphonate (EMS) Secara In Vitro
Yanti, Yulmira;
Habazar, Trimurti;
', Mardinus;
', Mansyurdin
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau
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DOI: 10.31258/jnat.11.2.104-108
The shoot of “rajasereh” banana was treated by 0,2% and 0,5% of ethyl methane sulfonate (EMS), for 2 and 4 hoursthourgh in vitro. The results showed that treatment of EMS mutagen would be changed morphologycal characterseither in planlet. In planlet stage was obtained four morphololgycal variations. One of the variant have characterizedthe colour yellowish; the leaves and stem are yellowish: the leaves were small and spiral. The value of coefficientof variant for morfphologycal characters of planlet “raja sereh” banana increased compare to the control. Theextreme value of coefficient of variant in planlet stage was found the times shoots is 84,31%, while control that is8,24%. EMS mutagen could caused several planlets shorter, total of leaves could of shoot are mostly
Efek Beta Karoten dan Agregasi Klorofil Pada Fotostabilitas Klorofil a dalam Pelarut Aseton
Costa, Junet. F. da;
Karwur, Ferry F;
Limantara, Leenawaty
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau
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DOI: 10.31258/jnat.11.2.115-123
Chlorophyll as photosynthetic pigment has many benefits to human such as antioxidant, antibacterial, color additive,immunity, and photosensitizer. Concerning to these applications, chlorophyll a will react with oxygen and light andreduce its effectiveness. It is then important to understand the stability of chlorophyll a on oxygen and light.Stability of chlorophyll a in the presence of beta-carotene (1:1) in acetone was studied for various waterconcentrations. Each solution was exposed to red light (ë e” 630 nm) for 0.5; 1.0; 2.5; 5.0; 10; 20; 30; 40; 50; and 60minutes and their spectrum were analysed. When water was added (8.33; 16.67; 25; 33.33; 41.67; 50; 58.33; 66.67;75; and 83.33 percent) to the acetone solution of chlorophyll a, the chlorophylls aggregate, and in the presence ofbeta-carotene, the chlorophyll more stable. The water shifts the chlorophyll a spectrum toward red duringillumination with or without the presence of carotene. The formation of oligomeric chlorophyll a aggregate occurswithin 10-20 minutes after the water was applied. This strategy of aggregating the chlorophylls to dissipateexcess energy captured from light does not support the photostability of chlorophyll as much as beta-carotene.Surprisingly, when beta carotene was mixed with 66.7% and 75.0% of water, the chlorophyll a degraded 5.56% and9.71% respectively. In conclusion, the aggregate form of chlorophyll a and the presence of beta-carotene increasephotostability of chlorophyll a in acetone solution.