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Life Science and Biotechnology
Published by Universitas Jember
ISSN : -     EISSN : 29884713     DOI : https://doi.org/10.19184/lsb
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Life Science and Biotechnology publishing rigorously peer-reviewed research that seeks to advance our understanding of fundamental processes in biology. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, and the public worldwide. Our journal publishes peer-reviewed research of broad interest in all areas of biology and at the interface of related disciplines (Genetics, Biodiversity, Biometry, Bioinformatics, Evolution, Ecology, Physiology, Biotechnology, Developmental biology, and Biomedical science).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 5 Documents
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Search results for , issue "Vol 1 No 2 (2023): November 2023" : 5 Documents clear
The HEMOLYSIS ACTIVITY OF BACTERIA ISOLATS FROM PELANGI FOREST OF IJEN GEOPARK Sattya Arimurti; esti Utarti; sutoyo sutoyo; siswanto siswanto; Tantri Raras Ayuningtyas
Life Science and Biotechnology Vol 1 No 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.44097

Abstract

Ijen geopark is one of Indonesia's geoparks, which is located in East Java. A total of 153 bacteria have been isolated from Pelangi Forest, which were then given the isolate code IHP. These bacterial isolates can be utilized in industries, including organic matter decomposer agents, plant biocontrol agents, and probiotics. To ensure these bacteria are safe to use in various fields, they must be non-disease-causing (non-pathogenic). Safety evaluations based on the hemolysis reactions offer simple tests to ease the analysis of potential pathogenic bacteria. The study aimed to evaluate the safety of bacterial isolates from Pelangi Forest for their hemolysis reactions. The hemolysis test was conducted using blood agar media, from which isolates with a negative (λ) reaction. Based on the results of hemolysis tests, 30 out of 153 bacterial isolates (19.60%) were found to be negative reactions. These bacteria are safe to proceed with for potential analysis.
Salmonella typhimurium injection as an immunostimulant: Study on chickens (Gallus gallus domesticus) Asmoro lelono; Rizky Surya
Life Science and Biotechnology Vol 1 No 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.43625

Abstract

Salmonella is a gram-negative bacteria commonly found in poultry-based products such as eggs and meat, which can affect quality and human health. The presence of these bacteria in poultry management can be traced to feed, water, substrate, and interactions with the environment. This study aims to investigate at the immune system of the avian at the age when the immune system begins to be produced independently through injection of S.typhimurium colonies. Salmonella culture at a density of 10-8 and 10-12 is injected into the digestive tract, and the increase in body temperature is measured immediately after injection. Two weeks later, an analysis of leukocyte differentiation is carried out. We found that salmonella injection affected body temperature and the rate of increase in body mass, both based on treatment and sex. This indicates that the immune system has worked even though the leukocyte differentiation indicators have not shown significant results. Chicks at the age of one month are able to show an immune response to bacterial infections through self-defence mechanisms. Further research needs to be carried out to understand whether leukocyte differentiation stimulates leukocyte variation with age.
The Diversity of Birds in Mangrove Forest at Biosite Pangpang Bay, Ijen Geopark Arif Mohammad Siddiq; Retno Wimbaningrum; Hari Sulistiyowati; Rendy Setiawan; Silvia Dwi Febrianti; Firman Syauqi Nur Sabila
Life Science and Biotechnology Vol 1 No 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.44165

Abstract

The Biosite Pangpang Bay Ijen Geopark (BPBIG) has a high potential biodiversity value outside the conservation area, particularly in the mangrove forest. Namun informasi keragaman ini masih belum sepenuhnya lengkap. Sehingga penelitian ini bertujuan untuk menentukan keragaman jenis burung di hutan mangrove BPBIG. The research was conducted on October 2023 using the point count method at six observation points in the mangrove forest. Data analysis was carried out in two approaches, i.e., species composition and species diversity index using Shannon Wiener index (H’). We recorded 39 bird species, consisting of 32 genera and 21 families, in the mangrove forest of BPBIG. There is one bird species that has important conservation status based on the IUCN Red List and National Regulation, namely L. javanicus (a vulnerable and protected species). Furthermore, there are also three migratory birds (T. sanctus, N. phaeopus, and A. hypoleucos) that use the mangrove forest of BPBIG as stopover sites during migration periods. The results of the Shannon Wiener index showed that the bird diversity in the BPBIG mangrove ecosystem is in the medium category (H' = 2.02). Finally, based on these results, it can be concluded that the bird species in the mangrove forest of BPBIG are quite diverse.
Poiploidy induction of Indonesian Black Rice Oryza sativa L. Var. Cempo Ireng with Bio-catharantine Ludfi Kurniawan; Alvina Nur Laili; Devi Silvia Angraeni; Salsabila Qurrotu ‘Ain; Dyah Retno Wulandari; Fuad Bahrul Ulum
Life Science and Biotechnology Vol 1 No 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.43753

Abstract

High demand on black rice Oryza sativa L. due to their high antioxydant content and better nutrition than the white rice. On the other hand this high price rice still less interest for the farmet to cultivate it. Therefore, improvement of plant characters was carried out through polyploid induction using the natural anti-mitotic compound bio-catharanthine. The purpose of this study was to test the effectiveness of bio-catharanthine in inducing polyploidy in Cemp ireng black rice. This study used a two-factor completely randomized design (CRD) method with three replications. The treatment factor of bio-catharanthine concentration was 1%, 1.5 %, 2 %, 2 %, 2.5 % and 3 % with the soaking time of 48 hours. The germination rate, ploidy level, stomatal size and density, and antioxidant were measuret to observed the characteristic of the mutan. The results showed that the treatments of 3 % bio-catharanthine enhanced the chromosome number of black rice Cempo ireng. Biocatharantine did not affected the germination rate through the treatments. Out mutants shows alternation on the stomatal size and density. The antioxidant of leaf sample did not changed after the treatment. Bio-catharanthine on high concentration migh be possible to be applied on polyploidy induction in black rice
in-silico PASS prediction for medicinal potency of antioxidant from Ranunculus japonicus Thunb. Yusrina Risky Amalini; Yusfi Afidah
Life Science and Biotechnology Vol 1 No 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.45078

Abstract

Ranunculus japonicus has been traditionally used to treat various ailments, including malaria, jaundice, gastric problems, toothache, and eye inflammation. Recent studies reported that the plant extract effectively treat rheumatoid arthritis due to its secondary metabolites with antioxidant activities, such as flavonoids and phenols. In this study, we analyzed the flavonoids and phenols from R. japonicus using in silico methods. The study determined the flavonoid and phenol contents based on existing literature. The antioxidant potential analysis was conducted using PASS (Prediction of Activity Spectra for Substances). The results indicate the presence of two flavonoid compounds, namely Apigenin 6,8-di-C-|A-L-Arabinopyranoside and 3-Methylquercetin, and three phenol compounds, namely 3-Hydroxytrisol 3-O-glucoside, 4-Hydroxy-3-methoxyacetate, and Hydroxytrisol. Two compounds exhibited high bioactive potential (Pa value > 0.7), two compounds showed medium bioactive potential (Pa value > 0.5), and one compound exhibited low bioactive potential (Pa value < 0.5). The most bioactive compounds were Apigenin 6,8-di-C-|A-L-Arabinopyranoside and 3-Methylquercetin. The compounds with medium bioactivity were 3-Hydroxytrysol 3-O-glucoside and 4-Hydroxy-3-methoxycinnamic. The PASS online analysis revealed the potential of antioxidants for medicinal potencies

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