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Life Science and Biotechnology
Published by Universitas Jember
ISSN : -     EISSN : 29884713     DOI : https://doi.org/10.19184/lsb
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Life Science and Biotechnology publishing rigorously peer-reviewed research that seeks to advance our understanding of fundamental processes in biology. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, and the public worldwide. Our journal publishes peer-reviewed research of broad interest in all areas of biology and at the interface of related disciplines (Genetics, Biodiversity, Biometry, Bioinformatics, Evolution, Ecology, Physiology, Biotechnology, Developmental biology, and Biomedical science).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 5 Documents
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Search results for , issue "Vol 2 No 1 (2024): May 2024" : 5 Documents clear
Growth and physiological adaptation of Ageratum conyzoides L. under salinity stress Yusi Ananda Putri; Edia Fitri Dwinianti; Ulfatul Inayah; Abdillah Maulana Farhan
Life Science and Biotechnology Vol 2 No 1 (2024): May 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i1.49086

Abstract

Physiological stress affects all plants, including salinity stress, which poses significant challenges. Ageratum conyzoides L. was selected as the subject of this study due to its reputed ability to thrive under various stress conditions, owing to its excellent adaptive capacity. The study employed salt solutions at concentrations of 1000 ppm, 3000 ppm, and 5000 ppm, administered as a single treatment over four weeks. The aim was to assess the impact of salinity stress on the growth, stomatal density, and chlorophyll content of A. conyzoides. Results indicate that salt treatment adversely affected the height of A. conyzoides. Stomatal density was highest at the 5000 ppm concentration, attributed to intensified transpiration in response to stress compared to lower concentrations. Chlorophyll content also showed an reduced at higher salt concentrations. These findings highlight the physiological responses of A. conyzoides to salinity stress, providing insights into its adaptation mechanisms under adverse environmental conditions.
DNA Barcode Characteristic of Dendrobium crumenatum based on ITS2 Nurfajri Eka Fitri; Mukhamad Su’udi .; Sindhi Wahidatul Ikrimah
Life Science and Biotechnology Vol 2 No 1 (2024): May 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i1.48632

Abstract

Dendrobium crumenatum, known as the pigeon orchid, is a type of orchid that is used as medicine by local people. Identification of D.crumenatum has limitations because it is similar to the orchid species D. heterocarpum. DNA barcoding is an alternative technique for identifying D.crumenatum using molecular markers. One of the molecular markers that is reliable and widely used in DNA barcoding is ITS2. This study aims to identify the ITS2 sequence as a molecular barcode effective for D.crumenatum. Genomic DNA isolation of Dendrobium crumenatum was carried out using the Cetyl Trimethyl Ammonium Bromide (CTAB) method and DNA amplification using PCR. The results showed that DNA barcoding research using the ITS2 sequence in D. crumenatum provides specific results to distinguish D. crumenatum from other Dendrobium species. The similarity in flower morphology in D. crumenatum and D. formosum proposed as the cause of close proximity shown in phylogenetic tree. Based on these results, the ITS2 sequence is highly recommended as a molecular marker for barcoding orchids, especially D. crumenatum since its capability for differentiating between species with morphological similarities. Key words: Dendrobium crumenatum, DNA Barcoding, ITS2
Characterization of Apyrase from the Salivary Glands of Ae. aegypti Based on Bioinformatics Database Syubbanul - Wathon; Alvina Nur Laili; Adriana Iflaha; Kaysha Rahmadita Widyadana; Zahniar Zahniar; Dyah Rahmadhani Saraswati; Rike Oktarianti; Kartika Senjarini
Life Science and Biotechnology Vol 2 No 1 (2024): May 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i1.48353

Abstract

The salivary glands of mosquitoes, which act as disease vectors, contain biological components that facilitate the blood-feeding process and transmit pathogens to the human body. Apyrase, a protein from the salivary glands of Ae. aegypti, is known to be an immunogenic protein that influences the host's immunity. It can prevent platelet aggregation by hydrolyzing ATP or ADP into AMP and thus facilitate the blood-feeding process. This mechanism is also exploited by pathogens to obtain entry into the host's body. Vaccination with the apyrase protein could control dengue virus transmission by enhancing the immune response to inhibit the pathogen's entry. To examine the potential of apyrase, it is critical to explore its nature and characteristics by using bioinformatics databases. This quantitative descriptive study involved apyrase protein from the salivary glands of Ae. aegypti obtained from several bioinformatics databases, namely UniProt, SWISSMODEL, ENZYME, Rhea, CFSSP, GlyCosmos, STRING, and QuickGo. The findings contribute to a more fine-cut understanding of apyrase as a potential vaccine to inhibit dengue transmission.
Prediction Prediction of the Insecticidal Potency of Biduri Plants (Calotropis gigantea) using the PASS online web resource Fahma Wardah Sururin; Nida An Khafiyya
Life Science and Biotechnology Vol 2 No 1 (2024): May 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i1.47444

Abstract

Calotropis gigantea, a wild plant thriving in arid environments, has been traditionally used for medicinal purposes by communities near Baluran National Park (BNP). The latex of C. gigantea is used as a crab poison due to its ability to cause fatal damage and separation of body parts, possibly related to chitin disruption in insects. This study explores the potential insecticidal properties of secondary metabolites in C. gigantea using Prediction of Activity Spectra for Substance (PASS) Online. Out of 68 identified secondary metabolites, six show significant insecticidal potential, namely Profenophos, Ethion, Alpha-Citral, 1-Phenylethyl acetate, (E)-dec-3-en-2-one, and Benzaldehyde. Notably, Profenophos, Ethion, Alpha-Citral, and Benzaldehyde exhibit toxic properties effective against insects, with enzyme inhibitory activity affecting nerve signaling and immune systems, suggesting potential for bio-insecticide development.
A Preliminary Study of The Javan Leopard Behaviour in Meru Betiri National Park Nur Kholiq; Adi Sucipto
Life Science and Biotechnology Vol 2 No 1 (2024): May 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i1.47666

Abstract

Meru Betiri National Park (MBNP, 526km2), is one of only three protected areas in east Java that can support a viable Javan leopard population. The Javan leopard has become a priority for management following the extinction of the Javan tiger. Therefore, it is important to know how the Javan Leopard behaves to improve species management. We conducted a field study using camera traps to determine the daily behavior of the Javan leopard in MBNP. A monitoring site was established with 73 stations, with a density of one station per 0.5 x 0.5 km grid cell, providing different but comparable densities and spatial coverage. The results indicate that the Javan leopard displays 9 daily behaviors including walking, observing, standing, cheek rubbing, scraping, urine spraying, claw marking, parenting, vocalizing, and others. Each behavior was observed with a relative frequency of walking (63.25%), observing (14.53%), standing (7.69%), cheek rubbing (5.98%), scraping (4.27%), urine spraying (1.71%), claw marking (0.85%), parenting (0.85%) and vocalizing (0.85%). An analysis of the time spent on each behavior revealed their proportion as follows: walking (382 s, 49.29%), watching (208 s, 26.84%), standing (66 s, 8.52%), cheek rubbing (27 s, 3.48%), scraping (57 s, 7.35%), urine spraying (17 s, 2.19%), vocalizing (9 s, 1.16%), claw marking (4 s, 0.52%) and parenting (5 s, 0.65%).

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