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Fuad Bahrul Ulum
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Life Science and Biotechnology
Published by Universitas Jember
ISSN : -     EISSN : 29884713     DOI : https://doi.org/10.19184/lsb
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Life Science and Biotechnology publishing rigorously peer-reviewed research that seeks to advance our understanding of fundamental processes in biology. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, and the public worldwide. Our journal publishes peer-reviewed research of broad interest in all areas of biology and at the interface of related disciplines (Genetics, Biodiversity, Biometry, Bioinformatics, Evolution, Ecology, Physiology, Biotechnology, Developmental biology, and Biomedical science).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 15 Documents
 1   2 
Growth and physiological adaptation of Ageratum conyzoides L. under salinity stress Yusi Ananda Putri; Edia Fitri Dwinianti; Ulfatul Inayah; Abdillah Maulana Farhan
Life Science and Biotechnology Vol 2 No 1 (2024): May 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i1.49086

Abstract

Physiological stress affects all plants, including salinity stress, which poses significant challenges. Ageratum conyzoides L. was selected as the subject of this study due to its reputed ability to thrive under various stress conditions, owing to its excellent adaptive capacity. The study employed salt solutions at concentrations of 1000 ppm, 3000 ppm, and 5000 ppm, administered as a single treatment over four weeks. The aim was to assess the impact of salinity stress on the growth, stomatal density, and chlorophyll content of A. conyzoides. Results indicate that salt treatment adversely affected the height of A. conyzoides. Stomatal density was highest at the 5000 ppm concentration, attributed to intensified transpiration in response to stress compared to lower concentrations. Chlorophyll content also showed an reduced at higher salt concentrations. These findings highlight the physiological responses of A. conyzoides to salinity stress, providing insights into its adaptation mechanisms under adverse environmental conditions.
In Silico Primer Design for geographical detection of Apis florea using Cytochrome c oxidase subunit 1 (COX1) gene Nurul Insani Shullia; Kuswati Kuswati; Aditya Kurniawan; Hajar Syifa Fiarani
Life Science and Biotechnology Vol 1 No 1 (2023): May
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i1.40052

Abstract

The yellow dwarf honey bee, Apis florea are well distributed in South Asia to South East Asia. This species is expanded and introduce area from their original distribution. However, the distribution of this honey bee in Indonesia is unexplored. The cytochrome c oxidase subunit 1 (COX1) gene are success to detect original geographic of introduce A. florea found in Egypt. The A. florea specific primer of COX1 gene are needed to produce the molecular marker for geographical origin detection. Thus, this study aims to in silico design the COX1 gene primer of A. florea using Primer3 and Primer-BLAST. This study results the best candidate primer of Af6_COX1_F and Af6_COX1_R primers that start from 212 to 983 regarding the A. florea COX1 gene (NC_021401) and produce 772 bp of amplicon. The melting temperature of forward primer are 54. 63 and 55.58°C, GC content of 40 and 45%, GC clamp of 3 and 2 for forward and reverse primer, respectively. There are no secondary structure of those primer and 100% homolog with A. florea COX1 sequences, thus those primer is potentially as the geographical origin marker of A. florea.
The HEMOLYSIS ACTIVITY OF BACTERIA ISOLATS FROM PELANGI FOREST OF IJEN GEOPARK Sattya Arimurti; esti Utarti; sutoyo sutoyo; siswanto siswanto; Tantri Raras Ayuningtyas
Life Science and Biotechnology Vol 1 No 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.44097

Abstract

Ijen geopark is one of Indonesia's geoparks, which is located in East Java. A total of 153 bacteria have been isolated from Pelangi Forest, which were then given the isolate code IHP. These bacterial isolates can be utilized in industries, including organic matter decomposer agents, plant biocontrol agents, and probiotics. To ensure these bacteria are safe to use in various fields, they must be non-disease-causing (non-pathogenic). Safety evaluations based on the hemolysis reactions offer simple tests to ease the analysis of potential pathogenic bacteria. The study aimed to evaluate the safety of bacterial isolates from Pelangi Forest for their hemolysis reactions. The hemolysis test was conducted using blood agar media, from which isolates with a negative (λ) reaction. Based on the results of hemolysis tests, 30 out of 153 bacterial isolates (19.60%) were found to be negative reactions. These bacteria are safe to proceed with for potential analysis.
The Composition and Diversity of Fresh Water Fish in The Upstream of the Bedadung River, Jember District Rendy Setiawan Setiawan
Life Science and Biotechnology Vol 1 No 1 (2023): May
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i1.38088

Abstract

The river is an irrational body of water from upstream to downstream. The upstream part of the river generally has a narrow width of the river flow, heavy air currents, the right and left banks of the river have vegetation trees, sandy rocky substrate types, and are located in the highlands or mountains. This character is also found in the Upper Bedadung River in the Argopuro Mountains. Characteristics in the upper reaches of the river are thought to affect the existence of creatures that live in it, such as fish. The purpose of this study was to determine the composition, dominance, and types of freshwater fish in the upper reaches of the Bedadung River, Jember Regency. The research method used is roaming method. The exploration was carried out at four research stations. The results showed that in the Upper S. Bedadung five species of fish were found including Channa gachua, Poecilia reticulata, Barbodes binotatus, Glyptothorax platypogon, and Nemacheilus fasciatus. The diversity of freshwater fish species in the upper reaches of the Bedadung River is moderate.
DNA Barcode Characteristic of Dendrobium crumenatum based on ITS2 Nurfajri Eka Fitri; Mukhamad Su’udi .; Sindhi Wahidatul Ikrimah
Life Science and Biotechnology Vol 2 No 1 (2024): May 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i1.48632

Abstract

Dendrobium crumenatum, known as the pigeon orchid, is a type of orchid that is used as medicine by local people. Identification of D.crumenatum has limitations because it is similar to the orchid species D. heterocarpum. DNA barcoding is an alternative technique for identifying D.crumenatum using molecular markers. One of the molecular markers that is reliable and widely used in DNA barcoding is ITS2. This study aims to identify the ITS2 sequence as a molecular barcode effective for D.crumenatum. Genomic DNA isolation of Dendrobium crumenatum was carried out using the Cetyl Trimethyl Ammonium Bromide (CTAB) method and DNA amplification using PCR. The results showed that DNA barcoding research using the ITS2 sequence in D. crumenatum provides specific results to distinguish D. crumenatum from other Dendrobium species. The similarity in flower morphology in D. crumenatum and D. formosum proposed as the cause of close proximity shown in phylogenetic tree. Based on these results, the ITS2 sequence is highly recommended as a molecular marker for barcoding orchids, especially D. crumenatum since its capability for differentiating between species with morphological similarities. Key words: Dendrobium crumenatum, DNA Barcoding, ITS2
Salmonella typhimurium injection as an immunostimulant: Study on chickens (Gallus gallus domesticus) Asmoro lelono; Rizky Surya
Life Science and Biotechnology Vol 1 No 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.43625

Abstract

Salmonella is a gram-negative bacteria commonly found in poultry-based products such as eggs and meat, which can affect quality and human health. The presence of these bacteria in poultry management can be traced to feed, water, substrate, and interactions with the environment. This study aims to investigate at the immune system of the avian at the age when the immune system begins to be produced independently through injection of S.typhimurium colonies. Salmonella culture at a density of 10-8 and 10-12 is injected into the digestive tract, and the increase in body temperature is measured immediately after injection. Two weeks later, an analysis of leukocyte differentiation is carried out. We found that salmonella injection affected body temperature and the rate of increase in body mass, both based on treatment and sex. This indicates that the immune system has worked even though the leukocyte differentiation indicators have not shown significant results. Chicks at the age of one month are able to show an immune response to bacterial infections through self-defence mechanisms. Further research needs to be carried out to understand whether leukocyte differentiation stimulates leukocyte variation with age.
Characterization of Apyrase from the Salivary Glands of Ae. aegypti Based on Bioinformatics Database Syubbanul - Wathon; Alvina Nur Laili; Adriana Iflaha; Kaysha Rahmadita Widyadana; Zahniar Zahniar; Dyah Rahmadhani Saraswati; Rike Oktarianti; Kartika Senjarini
Life Science and Biotechnology Vol 2 No 1 (2024): May 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i1.48353

Abstract

The salivary glands of mosquitoes, which act as disease vectors, contain biological components that facilitate the blood-feeding process and transmit pathogens to the human body. Apyrase, a protein from the salivary glands of Ae. aegypti, is known to be an immunogenic protein that influences the host's immunity. It can prevent platelet aggregation by hydrolyzing ATP or ADP into AMP and thus facilitate the blood-feeding process. This mechanism is also exploited by pathogens to obtain entry into the host's body. Vaccination with the apyrase protein could control dengue virus transmission by enhancing the immune response to inhibit the pathogen's entry. To examine the potential of apyrase, it is critical to explore its nature and characteristics by using bioinformatics databases. This quantitative descriptive study involved apyrase protein from the salivary glands of Ae. aegypti obtained from several bioinformatics databases, namely UniProt, SWISSMODEL, ENZYME, Rhea, CFSSP, GlyCosmos, STRING, and QuickGo. The findings contribute to a more fine-cut understanding of apyrase as a potential vaccine to inhibit dengue transmission.
Epiphytic ferns and orchids adaptation mechanism based on stomatal structure and chlorophyll content in Urban Forest of Jember University, Indonesia Fuad bahrul Ulum; Dwi Setyati; M. Su'udi
Life Science and Biotechnology Vol 1 No 1 (2023): May
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i1.39678

Abstract

Epiphytic orchids and ferns in urban forests simulating a lowland tropical forest ecology were studied. This study aims to provide information about the epiphytes' adaptation to living in a dynamic microclimate of the tree canopy. Five epiphytes from the two taxa (orchids and ferns) were observed in their stomatal structure and chlorophyll contents. The stomatal type, size, and density were analysed with microscopic observation. The chlorophyll content was measured using a spectrophotometer. The orchids developed different mechanisms for water conservation compared to the ferns. Orchis develop smaller sizes and denser stomata to support photosynthesis rate as they had water absorbers and storage organs. On the other hand, ferns tend to avoid drought stress by developing fewer numbers and sparse stomata. The higher chlorophyll content in ferns was for photosynthesis efficiency purposes
The Diversity of Birds in Mangrove Forest at Biosite Pangpang Bay, Ijen Geopark Arif Mohammad Siddiq; Retno Wimbaningrum; Hari Sulistiyowati; Rendy Setiawan; Silvia Dwi Febrianti; Firman Syauqi Nur Sabila
Life Science and Biotechnology Vol 1 No 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.44165

Abstract

The Biosite Pangpang Bay Ijen Geopark (BPBIG) has a high potential biodiversity value outside the conservation area, particularly in the mangrove forest. Namun informasi keragaman ini masih belum sepenuhnya lengkap. Sehingga penelitian ini bertujuan untuk menentukan keragaman jenis burung di hutan mangrove BPBIG. The research was conducted on October 2023 using the point count method at six observation points in the mangrove forest. Data analysis was carried out in two approaches, i.e., species composition and species diversity index using Shannon Wiener index (H’). We recorded 39 bird species, consisting of 32 genera and 21 families, in the mangrove forest of BPBIG. There is one bird species that has important conservation status based on the IUCN Red List and National Regulation, namely L. javanicus (a vulnerable and protected species). Furthermore, there are also three migratory birds (T. sanctus, N. phaeopus, and A. hypoleucos) that use the mangrove forest of BPBIG as stopover sites during migration periods. The results of the Shannon Wiener index showed that the bird diversity in the BPBIG mangrove ecosystem is in the medium category (H' = 2.02). Finally, based on these results, it can be concluded that the bird species in the mangrove forest of BPBIG are quite diverse.
Prediction Prediction of the Insecticidal Potency of Biduri Plants (Calotropis gigantea) using the PASS online web resource Fahma Wardah Sururin; Nida An Khafiyya
Life Science and Biotechnology Vol 2 No 1 (2024): May 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i1.47444

Abstract

Calotropis gigantea, a wild plant thriving in arid environments, has been traditionally used for medicinal purposes by communities near Baluran National Park (BNP). The latex of C. gigantea is used as a crab poison due to its ability to cause fatal damage and separation of body parts, possibly related to chitin disruption in insects. This study explores the potential insecticidal properties of secondary metabolites in C. gigantea using Prediction of Activity Spectra for Substance (PASS) Online. Out of 68 identified secondary metabolites, six show significant insecticidal potential, namely Profenophos, Ethion, Alpha-Citral, 1-Phenylethyl acetate, (E)-dec-3-en-2-one, and Benzaldehyde. Notably, Profenophos, Ethion, Alpha-Citral, and Benzaldehyde exhibit toxic properties effective against insects, with enzyme inhibitory activity affecting nerve signaling and immune systems, suggesting potential for bio-insecticide development.

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