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Fuad Bahrul Ulum
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Life Science and Biotechnology
Published by Universitas Jember
ISSN : -     EISSN : 29884713     DOI : https://doi.org/10.19184/lsb
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Life Science and Biotechnology publishing rigorously peer-reviewed research that seeks to advance our understanding of fundamental processes in biology. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, and the public worldwide. Our journal publishes peer-reviewed research of broad interest in all areas of biology and at the interface of related disciplines (Genetics, Biodiversity, Biometry, Bioinformatics, Evolution, Ecology, Physiology, Biotechnology, Developmental biology, and Biomedical science).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 15 Documents
 1   2 
Screening Ethanol-Producing Yeast Origin from Pineapple Honey Ananas comosus (L.) Merr var Quenn Sutoyo Sutoyo; Siswanto Siswanto
Life Science and Biotechnology Vol 1 No 1 (2023): May
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i1.39560

Abstract

The availability of fossil-based fuels will gradually decrease while the amount required increases. This has prompted research to find alternative energy sources, including bioethanol. Bioethanol, as an alternative energy source, can be used as a transportation fuel better than fossil-based fuels because ethanol has a high octane number, laminar velocity and heat of vaporisation. Meanwhile, the level of carbon and hydrocarbon emissions is low so it can increase combustion efficiency in vehicles. In addition, the use of bioethanol as fuel is environmentally friendly and renewable. Ethanol can be produced through a fermentation process using ethanol-producing yeast. Pineapple honey is known for its high sugar and other nutrient content, so it is supposed that many types of yeast can grow well in it. This study aims to isolation and screening of bioethanol-producing yeast from Pineapple Honey. This study was carried out by yeast isolation method, primary yeast screening, secondary yeast screening, and characterisation of selected yeast isolates. Data on ethanol production by yeast isolates were analysed by ANOVA with α=0.05 using the R studio program. This primary isolation and screening phase found eight yeast isolates capable of fermenting glucose and producing acid and gas in the test medium. Test ethanol production in DPE medium with 18% glucose content on all isolates showed that all isolates could produce ethanol with a range of 4.20-10.35%. Isolates of IK4 showed the highest ethanol production ability (10.35%) (p<0.5%) in significant contrast to the other seven isolates. Based on macroscopic and microscopic morphological characterisations, IKM 4 isolates show similarities to the genus Saccharomyces Keywords: ethanol, pineapple, yeasts
Poiploidy induction of Indonesian Black Rice Oryza sativa L. Var. Cempo Ireng with Bio-catharantine Ludfi Kurniawan; Alvina Nur Laili; Devi Silvia Angraeni; Salsabila Qurrotu ‘Ain; Dyah Retno Wulandari; Fuad Bahrul Ulum
Life Science and Biotechnology Vol 1 No 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.43753

Abstract

High demand on black rice Oryza sativa L. due to their high antioxydant content and better nutrition than the white rice. On the other hand this high price rice still less interest for the farmet to cultivate it. Therefore, improvement of plant characters was carried out through polyploid induction using the natural anti-mitotic compound bio-catharanthine. The purpose of this study was to test the effectiveness of bio-catharanthine in inducing polyploidy in Cemp ireng black rice. This study used a two-factor completely randomized design (CRD) method with three replications. The treatment factor of bio-catharanthine concentration was 1%, 1.5 %, 2 %, 2 %, 2.5 % and 3 % with the soaking time of 48 hours. The germination rate, ploidy level, stomatal size and density, and antioxidant were measuret to observed the characteristic of the mutan. The results showed that the treatments of 3 % bio-catharanthine enhanced the chromosome number of black rice Cempo ireng. Biocatharantine did not affected the germination rate through the treatments. Out mutants shows alternation on the stomatal size and density. The antioxidant of leaf sample did not changed after the treatment. Bio-catharanthine on high concentration migh be possible to be applied on polyploidy induction in black rice
A Preliminary Study of The Javan Leopard Behaviour in Meru Betiri National Park Nur Kholiq; Adi Sucipto
Life Science and Biotechnology Vol 2 No 1 (2024): May 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i1.47666

Abstract

Meru Betiri National Park (MBNP, 526km2), is one of only three protected areas in east Java that can support a viable Javan leopard population. The Javan leopard has become a priority for management following the extinction of the Javan tiger. Therefore, it is important to know how the Javan Leopard behaves to improve species management. We conducted a field study using camera traps to determine the daily behavior of the Javan leopard in MBNP. A monitoring site was established with 73 stations, with a density of one station per 0.5 x 0.5 km grid cell, providing different but comparable densities and spatial coverage. The results indicate that the Javan leopard displays 9 daily behaviors including walking, observing, standing, cheek rubbing, scraping, urine spraying, claw marking, parenting, vocalizing, and others. Each behavior was observed with a relative frequency of walking (63.25%), observing (14.53%), standing (7.69%), cheek rubbing (5.98%), scraping (4.27%), urine spraying (1.71%), claw marking (0.85%), parenting (0.85%) and vocalizing (0.85%). An analysis of the time spent on each behavior revealed their proportion as follows: walking (382 s, 49.29%), watching (208 s, 26.84%), standing (66 s, 8.52%), cheek rubbing (27 s, 3.48%), scraping (57 s, 7.35%), urine spraying (17 s, 2.19%), vocalizing (9 s, 1.16%), claw marking (4 s, 0.52%) and parenting (5 s, 0.65%).
The matK Mini-barcode as a Potential Molecular Identification Tool for Medicinal Orchids Mukhamad Su'udi; Dwi Setyati; Gita Ayu Khoirunnisa; Yusfi Afidah; Fuad Bahrul Ulum
Life Science and Biotechnology Vol 1 No 1 (2023): May
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i1.39851

Abstract

matK is one of the universal loci which is commonly used as a barcode in the molecular identification of plants. Up to now, the problem of matK as a single locus is the use of the entire gene region as barcodes. The long amplicon size of matK raises several problems in DNA barcoding. The aim of this study is to uncover a small region of the matK gene as a potential mini-barcode region for alternative sites to get more efficient and effective results. In silico studies performed include the collection of matK sequence of medicinal orchid from GenBank, the amplicon size from matK primer sets that are commonly used in the Orchidaceae family, and PCR analysis. Data analysis was done by comparing the results of PCR amplification from several primer sets: matK 390F-1326R, 743F-R2, 712F-1154R and 712F-1216R. The results show that all primer pairs are able to amplify the DNA template isolated from Dendrobium purpureum. However, the shorter DNA band obtained from matK 743F-R2, 712F-1154R and 712F-1216R primers sets were visualized more thick than the longer amplicon band produced from matK primer 390F-1326R. This indicates that short matK sequence for DNA barcoding (mini-barcode) is more efficient in amplifying DNA templates compared to generally applied barcoding using full length sequences.
in-silico PASS prediction for medicinal potency of antioxidant from Ranunculus japonicus Thunb. Yusrina Risky Amalini; Yusfi Afidah
Life Science and Biotechnology Vol 1 No 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.45078

Abstract

Ranunculus japonicus has been traditionally used to treat various ailments, including malaria, jaundice, gastric problems, toothache, and eye inflammation. Recent studies reported that the plant extract effectively treat rheumatoid arthritis due to its secondary metabolites with antioxidant activities, such as flavonoids and phenols. In this study, we analyzed the flavonoids and phenols from R. japonicus using in silico methods. The study determined the flavonoid and phenol contents based on existing literature. The antioxidant potential analysis was conducted using PASS (Prediction of Activity Spectra for Substances). The results indicate the presence of two flavonoid compounds, namely Apigenin 6,8-di-C-|A-L-Arabinopyranoside and 3-Methylquercetin, and three phenol compounds, namely 3-Hydroxytrisol 3-O-glucoside, 4-Hydroxy-3-methoxyacetate, and Hydroxytrisol. Two compounds exhibited high bioactive potential (Pa value > 0.7), two compounds showed medium bioactive potential (Pa value > 0.5), and one compound exhibited low bioactive potential (Pa value < 0.5). The most bioactive compounds were Apigenin 6,8-di-C-|A-L-Arabinopyranoside and 3-Methylquercetin. The compounds with medium bioactivity were 3-Hydroxytrysol 3-O-glucoside and 4-Hydroxy-3-methoxycinnamic. The PASS online analysis revealed the potential of antioxidants for medicinal potencies

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