cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Iman Rusmana
Contact Email
rusmana13@yahoo.com
Phone
+62217560536
Journal Mail Official
microbiology.indonesia@gmail.com
Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
Location
Kota tangerang,
Banten
INDONESIA
Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 398 Documents
 17   18   19   20   21 
Antimicrobial Bioactive Compounds of Snail Seromucoid as Biological Response Modifier Immunostimulator AGNES SRI HARTI; NONY PUSPAWATI; RAHAJENG PUTRININGRUM
Microbiology Indonesia Vol. 13 No. 2 (2019): June 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1945.349 KB) | DOI: 10.5454/mi.13.2.3

Abstract

Anti-microbial bioactive compounds from snail (Achatina fullica Ferussac) contained in snail seromucoid. It contains bioactive compounds such as glycans, peptides, glycopeptides, and chondroitin sulfate which can function as biological response modifiers (BRM) immunostimulators. Immunostimulators are compounds that can increase cellular immune responses in various ways, namely increasing the number and activity of T cells, NK cells, and macrophages and releasing interferons and interleukin. Immunostimulators are compounds that can increase cellular immune responses in various ways, namely increasing the number and activity of T cells, NK cells, macrophages and releasing interferons and interleukins.The purpose of this study was to analyze antimicrobial bioactive seromucoid compound of snail (Achatina fullica Ferrusac) as biological response modifiers (BRM) immunostimulators. The research methods based on experimental laboratory results with research stages including snail seromucoid isolation; antimicrobial activity; characterization physicochemical and profile of snail seromucoid proteins. The results of antimicrobial activity showed that 100% seromucoid concentrations had MIC (Minimal Inhibition Concentration) in Staphylococcus aureus, Candida albicans, and Pseudomonas aeruginosa. The physicochemical examination results showed specific gravity of 1.010; pH 8, -1 -1 -1g lucose 16 mg dL ; 9 mg dL cholesterol; protein 2.8 mg dL and negative heavy metals (Pb, Cu, Hg, Al). The results of the analysis of protein profiles showed that there were 3 subunits of proteins, range from 55 to 72 kDa and 1 specific protein sub unit of 43 kDa which was thought to be antimicrobial and biological response modifiers (BRM) immunostimulators.
Levels of TNF-α in PBMC (Peripheral Blood Mononuclear Cells) Induced by Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in vitro FITHRIYAH SJATHA; OKTIVIA CHANDRA MUSTIKA; BETI ERNAWATI DEWI; TJAHJANI MIRAWATI SUDIRO
Microbiology Indonesia Vol. 13 No. 2 (2019): June 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (709.517 KB) | DOI: 10.5454/mi.13.2.4

Abstract

Dengue infection is a global health problem with an increasing incidence every year and now endemic in more than 100 WHO countries. Dengue infection is caused by dengue virus (DENV) which is an RNA virus with positive single strand, with ±11kb genome size encoding 3 structural proteins, 7 non-structural proteins, and two Untranslated Region (UTR). NS1 protein is known to have a very important role in the development of severe DENV infection, by the direct effect causing host cells damage and indirect effect by activating immune response to induce the secretion of excess cytokines. This study aims to evaluate whether recombinant pcNS1 plasmids which have been proven expressing recombinant NS1 proteins in previous studies is able to induce cytokine secretion from Peripheral Blood Mononuclear Cells (PBMC). Transfected Chinese Hamster Ovary-K1 (CHO-K1) cells with recombinant pcNS1 plasmid was co-cultured with PBMC from healthy donor. After 48 h post co-cultured, cell supernatant was collected and TNF-α levels and NS1 recombinant were measured by ELISA. The results showed that recombinant NS1 protein was expressed in CHO-K1 mammalian cell line and able to induce TNF-α with higher levels compared to control.
Polyoxygenated Diterpene Produced by The Indonesian Marine Sponge Callyspongiasp. as an Inhibitor of the Human Pancreatic Cancer Cells VIQQI KURNIANDA; SUCI FARADILLA; SOFYATUDDIN KARINA; SRI AGUSTINA; MARIA ULFAH; CHITRA OCTAVINA; FARAH SYAHLIZA; MUHAMMAD RIZKI RAMADHAN; SYAHRUL PURNAWAN; MUSRI MUSMAN
Microbiology Indonesia Vol. 13 No. 2 (2019): June 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (986.522 KB) | DOI: 10.5454/mi.13.2.5

Abstract

The bioactive compound from the Indonesian Marine SpongeCallyspongia sp.isolated based on bioassay-guided separation with several steps of chromatography has been done. There are 4 major compound known as isocopalane diterpene derivatives together with a plausible new compound, isocopalanol, a polyoxygenated isocopalane diterpene. Isocopalanol has molecular weight [M+H]+ 412.576 m/z indicate that the compound has molecular formula C24H44O5 determined by LCMS-ESI. This compound has bioactivity IC50 = 0.1 μg / mL against human pancreatic cancer cell. 
Comparison of N-Acetyl-L-Cysteine-Sodium Hydroxide-Based and Modified Petroff's Decontamination Method for Mycobacterium tuberculosis Culture ARIYANI KIRANASARI; MUHAMMAD RAYHAN
Microbiology Indonesia Vol. 15 No. 3 (2021): September 2021
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (505.868 KB) | DOI: 10.5454/mi.15.3.5

Abstract

Indonesia is one of 22 countries with a high incidence of tuberculosis in the world, particularly related to TBHIV and MDR-TB cases. Contamination of normal flora from nasopharyngeal tract is the main problem to isolate Mycobacterium tuberculosis (MTB) from sputum. Therefore, a safe solution to decontaminate sputum without killing MTB bacilli is needed. N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH)-based method and Petroff's method, which was modified with NaOH (4%) are widely used in laboratories. In the present study, we will evaluate the different of these methods. Of the 110 sputum samples were collected from suspected cases of Pulmonary TB, and the decontamination of sputum by these methods was performed, after acid-fast bacillus (AFB) smear, then the samples were cultured in Lowensteins Jensen slant medium. The positive culture was validated by chromatography test for detecting the antigen of MPT-64 and PNB. Based on the investigation, it has been shown that neither NALC-NaOH (71%) nor modified Petroff's methods (66%) had a significant effect on the positivity rate of AFB smear. However, the contamination on culture was significantly higher in samples treated with NALC-NaOH (21%) compared to Modified Petroff methods (13%) (p< 0.05). In addition, the proportion of positive culture in NALC-NaOH was lower than modified Petroff. In conclusion, our study proved that modified Petroff methods are still more effective on sputum decontamination than NALC-NaOH based on the positivity rate of MTB culture. Though, not significantly different on AFB microscopic examination.
Anti Fungal Activity of Chitinolytic Bacteria Lysinibacillus fusiformis and Brevibacillus reuszeri Against The Fungal Pathogens Rhizoctonia solani and Fusarium oxysporum Mashuri Masri; Eka Sukmawaty; As Awalia Amir
Microbiology Indonesia Vol. 15 No. 4 (2021): December
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (729.924 KB) | DOI: 10.5454/mi.15.4.3

Abstract

Abstract. Chitinolytic bacteria can produce chitinase, reported as a biocontrol agent against plants. This research aims to see chitinolytic activity in inhibiting the growth of Rhizoctonia solani and Fusarium oxysporum. Anti fungal testing in dual culture test by growing each of the chitinolytic bacteria, Lysinibacillus fusiformis and Brevibacillus reuszeri, with the pathogenic fungi, F. oxysporum and R. solani, in Petri dishes containing Chitin Agar Media facing a distance of 3 cm. The results showed that chitinolytic bacterial isolates were capable inhibit the fungus by having the activity of each index inhibition of L. fusiformis isolates (30%), B. reuszeri (77%) against F. oxysporum, and R. solani fungi isolates (100%) for each chitinolytic bacterial isolate. Keywords : Anti fungal, Chitinolytic bacteria, Pathogenic fungi.
Antibacterial and Antioxidant Activities of Ginger Essential Oils Mega Ferdina Warsito; Febriana Untari; Anggia Prasetyoputri; Fauzy Rachman; Eris Septiana; Asep Bayu; Akhirta Atikana; Linda Sukmarini; Masteria Yunovilsa Putra
Microbiology Indonesia Vol. 15 No. 4 (2021): December
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1605.595 KB) | DOI: 10.5454/mi.15.4.1

Abstract

Ginger is a rhizomatous perennial herb that grows abundantly in tropical areas. It has been used around the world as a spice, flavoring agent, and ingredient in traditional medicine. Ginger essential oils (GEOs) are derivatives of ginger that can be found in various products used in daily life, such as food, pharmaceutical, and cosmetics. The present study analyzed the chemical compositions, antioxidant, and antibacterial activities of three commercially available GEOs. The compositions of GEOs were identified using the gas chromatography method. The antioxidant activity was evaluated using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2’-azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay methods. The antibacterial activity was determined using a disc diffusion assay based on the diameter of the inhibition zone (DIZ). The main compounds identified from the samples were zingiberene, α-curcumene, β-sesquiphellandrene, camphene, α-farnesene, β-bisabolene, α-pinene, and 3-carene. The IC50 values were found to be 5.3023 and 1.4504 mg/mL for GEO1; 0.9249 and 0.5276 mg/mL for GEO2; and 10.4463 and 3.3535 mg/mL for GEO3 when evaluated using DPPH and ABTS assay methods, respectively. All samples showed antibacterial activity against Staphylococcus aureus ATCC 13420 and Bacillus subtilis (collection of Indonesian Institute of Sciences), while only GEO2 and 3 displayed inhibitory effect against Escherichia coli ATCC 9637.
Relationship and structural diversity of bacterial manganese superoxide dismutases and the strategy for its application in therapy and cosmetics Debbie S. Retnoningrum; Aluicia Anita Artarini; Wangsa T. Ismaya; Abykhair Muhammad; Muhammad D. Fadilah; Ratna A. Utami
Microbiology Indonesia Vol. 15 No. 4 (2021): December
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2349.93 KB) | DOI: 10.5454/mi.15.4.2

Abstract

Manganese superoxide dismutase (MnSOD) from bacteria shares high amino acid sequence homology and nearly identical structure. Despite of that, their characteristics are diverse, which likely due to their bacterial origin and adaptation to the environment. Most importantly, their structural similarity extends to eukaryotic MnSOD, i.e. human. Therefore, structural study of bacterial MnSOD is relevant to its human SOD and henceforth for its use in human as a therapeutic agent or a cosmetic ingredient. Further, eukaryotic MnSOD occurs as a tetramer while almost all of the prokaryotic are dimeric. In this review, relationship between the amino acid sequences and structures of MnSOD as well as their origin and evolution is discussed. The structures of FeSOD and cambialistic SOD, which are MnSOD closest homologs, are visited as the comparison. This study provides an insight to potential safe application of bacterial MnSOD, including necessary modifications to obtain desired characteristics for applications in human.
Molecular Dynamics Analysis of Thermostable DNA Pol I ITB-1 CHANGI WONG; PETER PROKSCH; LEE TUNG TAN; SAMUEL LIHAN; AAZANI MUJAHID; MORITZ MÜLLER
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.1.3.1

Abstract

One of the thermostable enzymes, which has been widely used in the biotechnological research, is DNA polymerase. The coding sequence of local DNA Pol I gene from a local thermophilic bacterium, namely DNA Pol I ITB-1, has been cloned, sequenced, and overexpressed. However, study on thermostability of this enzyme is very limited. In the present study, thermostability of the protein was evaluated by thermal unfolding simulation at 300, 400, and 500 K. Our simulation revealed that the secondary and tertiary structures of the protein was not significantly affected by thermal perturbation at 300 K, but they were affected and even gradually unfolded by that perturbation at 400 and 500 K. Evaluation of the root mean square fluctuation (RMSF) of individual residues from the simulation at 400 and 500 K revealed the distribution of the thermostability regions in the protein structure. From the RMSF analysis at 400 K, we found that thermostability of the 3&rsquo;-5&rsquo; exonuclease domain was lower compared to that of the other domains. Where as from the RMSF analysis at 500 K, we found that in each domain of DNA pol I ITB-1 there was a single extraordinary thermostable a-helix which was likely to be the core of each corresponding domain. Thus our simulation provides a thermostability map of DNA Pol I ITB-1. Such information will be very valuable for the next genetic engineering work in determining a mutation target to modify thermostability of this enzyme.
Production of Recombinant Vaccine Cb Peritrophin-42 of Screwworm Fly in Escherichia coli and Saccharomyces cerevisiae
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.1.3.2

Abstract

The screwworm fly (Chrysomya bezziana) larva is an obligate parasite which causes myasis in mammals. Vaccination is thought to be a protective and an enviromentally friendly method for combating the pest. A gene encoding a peritrophic membrane protein Cb peritrophin-42 of C. bezziana was cloned and expressed in Escherichia coli and Saccharomyces cerevisiae.Cb peritrophin-42 fused with the outer membrane protein A signal sequence was produced as an aggregate in E. coli. Expression of an Cb peritrophin-42 gene fused with oligonucleotide of the invertase signal sequence in S. cerevisiae allowed the production of 14.4 mg l-1 soluble extracellular Cb peritrophin-42. Sheep vaccinated with recombinant Cb peritrophin-42 showed a strong immunological reaction. In vivo assay following vaccination with the recombinant Cb peritrophin-42 showed a 27% reduction in the weight of recovered larvae.
Thermostable Chicken Feather Degrading Enzymes from L-23 Isolate from Indonesia ROSA SURYANTINI; REINE SUCI WULANDARI; RINA SRI KASIAMDARI
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.1.3.3

Abstract

The thermostable chicken feather degrading protease enzymes used here was extracted and partially purified from thermophilic bacteria L-23 isolated from a coastal hot spring in North Sulawesi, Indonesia. The L-23 was grown in the selective medium containing 1% chicken feather powder at 70 &deg;C and pH 7. The cell-free culture was precipitated with ammonium sulphate at 80% saturation, followed by heating at 65 &deg;C for 1 h before applied onto Sephadex G-100. The molecular weight of the two enzymes identified were estimated as 47 and 64 kDa. The optimum pH of the mixed enzymes preparation was 7 while the optimum temperature was 65 &deg;C. Zymogram analysis showed that one of the enzymes was still active after being heated at 100 &deg;C for 20 min and was also resistant towards organic solvents and SDS. The activity was enhanced by addition of 1 mM FeCl3.

Page 19 of 40 | Total Record : 398

 17   18   19   20   21 

Filter by Year

2007 2023


Reset
Filter By Issues
All Issue Vol. 17 No. 2 (2023): June Vol. 17 No. 1 (2023): March Vol. 16 No. 2 (2022): December Vol. 16 No. 1 (2022): March Vol. 15 No. 3 (2021): September 2021 Vol. 15 No. 2 (2021): June 2021 Vol. 15 No. 1 (2021): March 2021 Vol. 15 No. 4 (2021): December Vol. 14 No. 4 (2020): December 2020 Vol. 14 No. 3 (2020): September 2020 Vol. 14 No. 2 (2020): June 2020 Vol. 14 No. 1 (2020): March 2020 Vol. 13 No. 4 (2019): December 2019 Vol. 13 No. 3 (2019): September 2019 Vol. 13 No. 2 (2019): June 2019 Vol. 13 No. 1 (2019): March 2019 Vol. 12 No. 3 (2018): September 2018 Vol. 12 No. 2 (2018): June 2018 Vol. 12 No. 1 (2018): March 2018 Vol. 11 No. 4 (2017): December 2017 Vol. 11 No. 3 (2017): September 2017 Vol. 11 No. 2 (2017): Juni 2017 Vol. 11 No. 1 (2017): March 2017 Vol. 10 No. 4 (2016): December 2016 Vol. 10 No. 3 (2016): September 2016 Vol. 10 No. 2 (2016): June 2016 Vol. 10 No. 1 (2016): March 2016 Vol. 9 No. 4 (2015): December 2015 Vol. 9 No. 3 (2015): September 2015 Vol. 9 No. 2 (2015): June 2015 Vol. 9 No. 1 (2015): March 2015 Vol. 8 No. 4 (2014): December 2014 Vol. 8 No. 3 (2014): September 2014 Vol. 8 No. 2 (2014): June 2014 Vol. 8 No. 1 (2014): March 2014 Vol. 7 No. 4 (2013): November 2013 Vol. 7 No. 3 (2013): September 2013 Vol. 7 No. 2 (2013): June 2013 Vol. 7 No. 1 (2013): March 2013 Vol. 6 No. 4 (2012): December 2012 Vol. 6 No. 3 (2012): September 2012 Vol. 6 No. 2 (2012): June 2012 Vol. 6 No. 1 (2012): March 2012 Vol. 5 No. 4 (2011): December 2011 Vol. 5 No. 3 (2011): September 2011 Vol. 5 No. 2 (2011): June 2011 Vol. 5 No. 1 (2011): March 2011 Vol. 4 No. 3 (2010): December 2010 Vol. 4 No. 2 (2010): August 2010 Vol. 4 No. 1 (2010): April 2010 Vol. 3 No. 3 (2009): December 2009 Vol. 3 No. 2 (2009): August 2009 Vol. 3 No. 1 (2009): April 2009 Vol. 2 No. 3 (2008): December 2008 Vol. 2 No. 2 (2008): August 2008 Vol. 2 No. 1 (2008): April 2008 Vol. 1 No. 3 (2007): December 2007 Vol. 1 No. 2 (2007): August 2007 Vol. 1 No. 1 (2007): April 2007 More Issue