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Contact Name
Iman Rusmana
Contact Email
rusmana13@yahoo.com
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+62217560536
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microbiology.indonesia@gmail.com
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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
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Kota tangerang,
Banten
INDONESIA
Microbiology Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Articles 398 Documents
Detection of Liberibacter asiaticus causing Citrus Vein Phloem Degeneration from Siam Citrus leaves (Citrus nobilis var. microcarpa) in Singkawang City plantation, Pontianak, West Kalimantan RAHMAWATI RAHMAWATI; ILIANA ILIANA; AGUS RACHMAT; LATIFFAH ZAKARIA; MUKARLINA MUKARLINA
Microbiology Indonesia Vol. 14 No. 3 (2020): September 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (962.672 KB) | DOI: 10.5454/mi.14.3.2

Abstract

The objective of the present study was to detect presence of pathogenic fastidious bacterium, Candidatus Liberibacter asiaticus using PCR from leaves of Siam citrus showing Citrus Vein Phloem Degeneration (CVPD) symptoms in Singkawang City plantation, Pontianak, West Kalimantan, Indonesia. Citrus leaf samples were collected based on visual observation of symptoms showing CVPD infection. Typical symptoms of CVPD include leaf yellowing (chlorosis), vein banding, leaves become stiff, thicker and smaller in size. The pathogenic bacterium, Candidatus Liberibacter asiaticus was detected using two specific primers, namely, OI1/ OI2c amplified 16S rRNA gene and A2/J5, amplified ribosomal protein gene of the rplKAJL-rpoBC operon (β-operon). PCR amplification detected the presence of 1100 bp band using OI1/ OI2c primers, and 703 bp band using A2/J5 primers from symptomatic Siam citrus leaves. PCR products were not detected from healthy plants serve as control. By using two sets of specific primers to amplify 16S rRNA gene and ribosomal protein gene, Candidatus Liberibacter asiaticus was detected in symptomatic Siam citrus leaves in Singkawang City, Pontianak, Indonesia. Detection of the bacterial pathogen causing CVPD is important to prevent spreading of the disease which could affect production of citrus fruits.
Enhanced Ethanol Production by High Temperature-Tolerance Mutant Pichia kudriavzevii T-T2 in various Carbon and Nitrogen Sources Monica Ina Martha; Rika Indri Astuti; Wulan Tri Wahyuni
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.767 KB) | DOI: 10.5454/mi.14.2.3

Abstract

Previously, we had constructed proline accumulating Pichia kudriavzevii yeast mutant (T-T2) that exhibited high temperature tolerance phenotype. In this study we then analyzed the ethanol production of the particular mutant isolate in various carbon (C) and nitrogen sources (N). Fermentation rate of T-T2 either in 4% glucose or substrate mixture (SM = 2%glucose : 2% xylose) was higher than WT strain. The highest CO2 evolving rate by T-T2 in glucose and SM was 57.0 ml/hour and 51.4 ml/hour, respectively, suggesting that T-T2 was capable of using xylose as C-source. T-T2 resulted significant increase of total evolved CO2 than WT strain in glucose as substrate. T-T2 isolate was capable of producing higher ethanol than its WT strains in various C-sources, including glucose, maltose, sucrose, xylose and SM. However, glucose was found as the most preferable C-source to produce ethanol (3.2%) whereas maltose was the least preferable one. T-T2 was found capable in using inorganic sources (NaNO3) to produce ethanol (2.4%) as compared to other N-sources (urea, (NH4)2SO4, NH4Cl). Ethanol production by T-T2 strain in NaNO3 was 16% higher than its WT strain. This data indicate, that NaNO3 is potentially applied as N source alternative to produce ethanol. Based on our study, mutant strain T-T2 shows higher fermentation rate than its WT strain in various sugar and N-sources. The fact that T-T2 could produce ethanol from SM in a similar fermentation rate to single glucose as substrate, suggesting its potential application in the 2nd generation of bioethanol productions.
Synergistic Interaction of Arbuscular Mycorrhizal Fungi and Phosphate-Solubilizing Bacteria with NPK Fertilizer to Improve Sorghum bicolor (L.) Moench Growth under Saline Condition Indriati Ramadhani; Sri Widawati
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1355.827 KB) | DOI: 10.5454/mi.14.2.4

Abstract

ABSTRACT Sweet sorghum (Sorghum bicolor (L.) Moench) is a food source and a raw material in biofuel-ethanol production. Arbuscular mycorrhizal fungi (AMF) and phosphate-solubilizing bacteria (PSB) are called rhizosphere microorganisms, which are useful microorganisms that enhance plant growth. Rhizosphere microorganisms also increase plant's resistance to environmental stress, while NPK fertilizer used on agricultural land are found to increase crop yields only. However, its continuous application has a negative impact on the environment. Therefore, this research aimed to study the synergy between AMF, and PSB with NPK fertilizer in influencing the sweet sorghum growth in saline condition. Two treatment factors were used, which are microbes combination (arbuscular mycorrhizal fungi and phosphate-solubilizing bacteria), and NPK doses without NPK, 25% NPK, 50% NPK, and 100% NPK. Furthermore, zeolite was used to grow sweet sorghum seeds individually and were maintained for one month in a greenhouse. Pots were watered with 50% seawater (freshwater: seawater = 1:1) every day to keep the moisture. Plant growth parameters were also measured, which includes AMF colonization in the roots, number of AMF spores, and PSB population in the planting medium. The combination of AMF, PSB, and NPK in sweet sorghum increased the plant height, number of leaves, plant fresh weight, plant dry weight, and total of plant P, although not always significant. AMF+PSB+25% NPK produced the highest number in all parameters. Therefore, the synergy between AMF, and PSB with NPK fertilizer is able to increase the plant growth in saline condition. Key words: arbuscular mycorrhizal fungi, phosphate-solubilizing bacteria, NPK, saline, and sweet sorghum ABSTRAK Sorgum manis (Sorghum bicolor (L.) Moench) merupakan sumber makanan dan bahan baku dalam produksi bioetanol. Jamur mikoriza arbuskular (JMA) dan bakteri pelarut fosfat (BPF) disebut sebagai mikroorganisme rizosfer, yang merupakan mikroorganisme yang berguna untuk meningkatkan pertumbuhan tanaman. Mikroorganisme rizosfer juga meningkatkan ketahanan tanaman terhadap cekaman lingkungan, sedangkan pupuk NPK yang digunakan pada lahan pertanian ternyata hanya meningkatkan hasil panen saja. Namun, penerapannya yang berkelanjutan memiliki dampak negatif terhadap lingkungan. Oleh karena itu, penelitian ini bertujuan untuk mempelajari sinergi antara JMA, dan BPF dengan pupuk NPK dalam mempengaruhi pertumbuhan sorgum manis dalam kondisi salin. Dua faktor perlakuan yang digunakan, yaitu kombinasi mikroba (jamur mikoriza arbuskular dan bakteri pelarut fosfat), dan dosis NPK tanpa NPK, 25% NPK, 50% NPK, dan 100% NPK. Selanjutnya, zeolit digunakan untuk menumbuhkan biji sorgum manis secara individu dan dipelihara selama satu bulan di rumah kaca. Pot disiram dengan 50% air laut (air tawar: air laut = 1: 1) setiap hari untuk menjaga kelembaban. Parameter pertumbuhan tanaman juga diukur, yang meliputi kolonisasi JMA di akar, jumlah spora JMA, dan populasi BPF dalam media tanam. Kombinasi JMA, BPF, dan NPK dalam sorgum manis meningkatkan tinggi tanaman, jumlah daun, berat basah tanaman, berat kering tanaman, dan P total tanaman, meskipun tidak selalu signifikan. JMA + BPF + 25% NPK menghasilkan nilai tertinggi di semua parameter pertumbuhan. Oleh karena itu, sinergi antara JMA dan BPF dengan pupuk NPK mampu meningkatkan pertumbuhan tanaman dalam kondisi salin. Kata kunci: jamur mikoriza arbuskular, bakteri pelarut fosfat, NPK, salin, dan sorgum manis
QUALITY OF AIR BACTERIA IN OPERATING THEATER IN SOME HOSPITALS IN JAKARTA AND SURROUNDINGS AREAS IN 2018-2019 CONNY RIANA TJAMPAKASARI; NABILA NAURA; TJAHJANI MIRAWATI SUDIRO
Microbiology Indonesia Vol. 14 No. 4 (2020): December 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (828.971 KB) | DOI: 10.5454/mi.14.4.3

Abstract

Nosocomial infection is an infection obtained by a patient or hospital staff while in hospital. This infectionplays a role in causing morbidity and mortality in hospitals and can occur in various hospitals rooms, includingoperating theaters. Nosocomial infections can occur due to various factors, one of which is contamination fromairborne bacteria. In some countries, regulations are set to limit the concentration of airborne bacteria, both in theoperating theaters and other rooms in hospitals, hence the need for monitoring and supervision of air quality asareflection on the cleanliness conditions in hospitals. Based on this, it is necessary to know the bacteriological airquality in the operating theaters in several hospitals in Jakarta and surrounding areas as one of the steps to preventnosocomial infections. The method uses an air sampler with the principle of impaction. Air sampler works byseparating the particles from the air by utilizing the inertia of the particles to force the bacteria to settle to thesurface of the medium. A total of 217 examinations in the operating theaters were carried out in 17 hospitals inJakarta and surrounding areas during January 2018 to June 2019. The majority of the operating theaters inhospitals in Jakarta and surrounding areas have air quality that met appropriate quality standards. In 2018, 120 of137 (87.59%) examination in the operating theaters met the quality standar. Meanwhile in 2019, 70 of 80(87.50%) operating theaters met the standard determined by the Ministry of Health of the Republic of Indonesia. Key words: air sampler method, operating theater, quality of air bacteria
Isolation and Characterization of Thermophilic Bacteria as Cellulolytic Enzyme Producer from the Hot Spring of Ie Seuum Aceh Besar, Indonesia RUHUL KHALILA; lenni fitri; SUHARTONO SUHARTONO
Microbiology Indonesia Vol. 14 No. 1 (2020): March 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1580.621 KB) | DOI: 10.5454/mi.14.1.4

Abstract

Cellulase enzymes can be isolated from thermophile bacteria obtained from the hot spring Ie Seuum, Aceh Besar. This research aimed to recover and characterize the isolates morphologically and biochemically followed by determination of the thermophile bacterial isolates potential as cellulolytic enzyme producers,. The sampling method in this research was conducted by a purposive sampling at temperature of 70 oC, 60 oC and 50 oC. Isolation of thermophilic bacteria was carried out on nutrient agar (NA) media. There were four isolates of thermophilic bacteria isolated recovered at 70 oC, five isolates at 60 oC, and seven isolates at 50 oC. Of the 18 isolates obtained, 15 of them were able to produce cellulase enzymes. Cellulase enzyme production can be determined by the presence of clear zones around bacterial colonies on CMC media after addition of 1% congo red drops and wash with 1 M NaCl. The highest five Cellulolytic Index (CI) values ​​were obtained from isolates ISB75; ISB64; ISB52; ISB54; ISB56 that were 1.23; 2.22; 1.39; 1.59; 1.10, respectively. Biochemical tests carried out on 5 isolates with the highest cellulolytic index values showed that the bacterial isolate were suspected to be from the genera of Bacillus sp.
Detection of Salmonella sp. and Escherichia coli on Chicken Meat at Tamiang Layang Market: Detection of Salmonella sp. and Escherichia coli on Chicken Meat at Tamiang Layang Market AKHMAD RIZALDI; ENGKI ZELPINA
Microbiology Indonesia Vol. 14 No. 3 (2020): September 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (520.265 KB) | DOI: 10.5454/mi.14.3.5

Abstract

Salmonella sp. and Escherichia coli (E. coli) are the two most important pathogens because they are indicators of food safety and sanitation indicators because they can potentially pose a high risk of foodborne disease. This study aims to look at the prevalence of Salmonella sp. and E. coli in the Tamiang Layang Market as supervision of food safety. A total of 6 chicken breast samples were taken at all chicken traders in the Tamiang Layang market. Testing the presence of Salmonella sp. and E. coli using MC-Media Pad. The existence of Salmonella sp. and E. coli in chicken meat at the Tamiang Layang market were 66.6% and 83.3%. The need to improve the hygiene and sanitation of chicken traders.
In Silico Study on Testing Antidiabetic Compounds Candidate from Azaphilone Mold Monascus sp. Anna Yuliana; Hilman Fitriaji S P; Khofi Siti Mukhaufillah; Lina Rahmawati Rizkuloh
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1556.808 KB) | DOI: 10.5454/mi.14.2.2

Abstract

Monascus sp. is a mold that is used for rice fermentation so that the brown rice is produced (Red Mold Rice) or Angkak. This rice has long been used on Asian food and traditional medicine. Angkak contains many bioactive compounds, including monakolin which is potentially to be used as nutraceutical. Monascus sp. produced pigments include yellow (Ankaflavin, Monaskin), Orange (Rubropunktatin, Monaskorubrin) and Red (Rubropunktamin, monaskorubramin) which have various biological activities. The purpose of the study was to know the pigments of the Monascus sp. as a compound antidiabetic candidate. This includes the Azaphilone Kapang Monascus sp. derivative compound In Silico. The test compound consists of 57 compounds of Monascus sp. Glycogen Fosforilase used as an antidiabetic receptor. Ligan preparation is drawn using ChemDraw software then with Marvin Sketch done Protonasi. Testing Drug Scan (Screening ligand based Drug likeness). The preparation of receptors by downloading antidiabetic receptors in Protein Data Bank (GDP). The ADME study was conducted with PreADMET web-based software. Validate the docking method and molecular tethering using the Autodock software 4.2.6. Drugscan test results on compounds Isolate MPs4 have qualified grades in all parameters such as molecular weight, proton donor, proton acceptor, log p and molar refractory. The results of the ADME test in the compound Isolate MPs4 have a qualified value in all parameters Caco-2, HIA (Human Intestinal Absorption), as well as in the PPB (Protein Plasma Binding). The result of the docking test in the Isolate compound MPs4 to be the best and qualified compound because it has a smaller affinity binding than the natural ligands and the comparator ligand (glibenclamide). The result of this research Isolate MPs4 is the candidate for new drugs antidiabetic. Keyword : Monascus sp., In silico, Azaphilone, Docking, Antidiabetic
Antibiotic Sensitivity Pattern Among Diabetic Outpatients with Urinary Tract infection in Pontianak Mardhia Mardhia; Mahyarudin Mahyarudin; Abror Irsan
Microbiology Indonesia Vol. 14 No. 3 (2020): September 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.13 KB) | DOI: 10.5454/mi.14.3.1

Abstract

Diabetic patients are associated with a higher risk of infection. The research purposed to identify antibiotic susceptibility patterns among diabetic outpatients with urinary tract infection in Pontianak. An experimental study was performed for 13 bacterial isolates of diabetic outpatients with urinary tract infection in the Clinic of Diabetes Mellitus, Sultan Syarif Mohamad Alkadrie Hospital, Pontianak. The disc diffusion method was used to perform the susceptibility of antibiotics to the bacterial isolates. Among 13 isolates, the most common causative agent of urinary tract infection was Escherichia coli (53.85%), followed by Pseudomonas aeruginosa (30.77%). Klebsiella spp and Enterobacter aerogenes were 7.69%. Most isolates of bacteria of the study had a high sensitivity to Cefepime (92.31%), then followed by Levofloxacin, Amikacin, and Meropenem for 84.62%. The study revealed low sensitivity of bacteria to Amoxicillin/Clavulanate, Co-Trimoxazole, Cefazoline and Ceftriaxone (30.77%, 23.08 %, 23.08%, 23.08%, respectively). All bacterial isolates had high resistance to Ampicillin. Moreover, multidrug resistance observed among bacterial isolates. Keywords: antibiotic susceptibility, diabetes, urinary tract infections
ITA registration form and back cover Is Helianti
Microbiology Indonesia Vol. 13 No. 3 (2019): September 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1664.726 KB)

Abstract

Trenching Construction in Peat Soil and the DGGE Analyses of nif Gene and Activity of Dehydrogenase HAPPY WIDIASTUTI; DARMONO TANIWIRYONO; IMAN RUSMANA; GALUH WENING PERMATASARI
Microbiology Indonesia Vol. 14 No. 4 (2020): December 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2068.239 KB) | DOI: 10.5454/mi.14.4.2

Abstract

Basal stem rot (BSR) is a major disease in oil palm crops which also possible happen on peat soils. Ganoderma boninense is the pathogen that causes this disease infects the plants through the root. Maintaining root health expects to reduce the intensity of the occurrence of BSR disease and its spread. In this research, trenching construction and maintenance is carried out which aims to break the spread and activity of Ganoderma. Trenching maintenance is carried out by giving empty bunches oil palm inoculated with decomposer, Azotobacter inoculants and humic acid. Four treatments tested were trenched (T1) and untrenched (T0) in combination with two level of Ganoderma attack, which are moderate (Mo) and severe (Se). The soil sample were retrieved from different point, which are in the trenching area (TR), harvest path zone (H), and frond stack zone (M). The soil characteristic including microbe abundance, dehydrogenase activity, and PCR-DGGE of microbe community in each treatment were analyzed to reveal the effect of trenching construction. The results showed that trenching implementation reveals major results related to the abundance of microbes and fungi population, supported by the enhancement of dehydrogenase activity at the block with moderate Ganoderma attack. In addition, the DGGE study effectively separates the microbial population of four separate treatment into two clusters, splitting the grouping depending on the Ganoderma level attack. This study shows enhancement of soil characteristics biologically and nutrients status of oil palm leaves especially P, as the results of trenching construction in peatland. Keywords: peatland; oil palm; trenching, DGGE; microbes

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