cover
Contact Name
Knowledge Management Department
Contact Email
kmd@biotrop.org
Phone
+62251-8323848
Journal Mail Official
biotropia@biotrop.org
Editorial Address
SEAMEO BIOTROP Jl. Raya Tajur Km. 6 Bogor 16134 INDONESIA
Location
Kota bogor,
Jawa barat
INDONESIA
BIOTROPIA - The Southeast Asian Journal of Tropical Biology
ISSN : 02156334     EISSN : 1907770X     DOI : http://dx.doi.org/10.11598/btb
BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.
Articles 444 Documents
GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF Alcaligenes javaensis JG3 POTENTIAL AS AN EFFECTIVE BIODEGRADER Stalis Norma Ethica; Oedjijono Oedjijono; Endang Semiarti; Jaka Widada; Tri Joko Raharjo
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 1 (2018)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.448 KB) | DOI: 10.11598/btb.2018.25.1.583

Abstract

Utilization of glycerol by lipase producing bacteria offers great benefits for fat and oil waste degradation and waterwaste treatment. Nevertheless, there have been lack of reports about the availability of non-pathogenic, lipase producing bacteria, which could naturally degrade glycerol produced from the lipolysis process by lipase. This study reported a newly identified species of rhizobacteria, Alcaligenes javaensis JG3, which is not only able to produce high level of lipase, but also able to degrade glycerol molecules. Identification of strain JG3 was carried out using SEM (Scanning Electron Microscope), BD Phoenix 100 Automated Microbiology System and 16S rRNA gene analysis to determine its taxonomy status. The ability of the strain to metabolize glycerol was investigated both genotypically and phenotypically using degenerate PCR and a glycerol minimal medium. Identification test results showed that strain JG3 belongs to genus Alcaligenes, with the closest relationship with A. faecalis and A. aquatilis (96% nucleotide similarity maximum). Degenerate PCR resulted in a 248-bp sequence showing 93% similarity with glpK of Candidatus Sodalis pierantonius SOPE, a key gene involved in glycerol metabolism. In vitro glycerol utilization test result showed that Alcaligenes sp. JG3 was able to grow on glycerol aerobically, but not anaerobically. It is concluded that Alcaligenes sp. JG3 possesses genes coding for glycerol metabolism and this trait is phenotypically expressed, thus making the strain potential to be used as an effective fat and oil biodegrader.
CHROMOSOMAL CHARACTERS OF THE INDONESIAN SAND GOBY, OXYELEOTRISMARMORATA BLKR. 1874 (ELEOTRIDAE)*) JIMMY T. MASAGCA
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 7 (1994)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.876 KB) | DOI: 10.11598/btb.1994.0.7.112

Abstract

Karyomorphological investigation of sand goby or marble sleeper (Oxyeleotris marmorata Blkr.) from Cirata Reservoir, West  Java,  Indonesia was  undertaken  to  determine  the modal  chromosome  number  and  fundamental number, and to construct the karyotype from somatic metaphase cells of head kidney. A total of 30 fish samples from  Cirata  Reservoir  was  sacrificed  for  direct  chromosome  preparation  by colchicine-citrate-aceto-methanol-Giemsa staining-air drying  technique. Chromosome set analysis showed  that  the modal chromosome number of the test fish is 2n = 46, confirming previous studies. Fundamental number is 50. Two karyotypic formulas were found, i.e. 1) 2n = 46 (4SM + 42A); and 2) 2n = 46 (2M + 2SM + 42A).  Key Words: Freshwater fishes, Chromosome analysis, Oxyeleotris marmorata, Indonesia
POSTHARVEST QUALITY IMPROVEMENT OF SORGHUM (Sorghum bicolor (L.) Moench) GRAINS OKKY SETYAWATI DHARMAPUTRA
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 19 No. 2 (2012)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.92 KB) | DOI: 10.11598/btb.2012.19.2.255

Abstract

The objectives of this study were (a) to investigate the effect of postharvest handling (threshing and storage) methods on the quality of sorghum (Sorghum bicolor (L) Moench) grains variety Numbu, in terms of the percentages of damaged grains and seed germination, population growth of S. zeamais, F. proliferatum and F. verticillioides; fumonisin B1 and carbohydrate contents, and the percentage of weight loss during storage. The change of moisture contents of sorghum grains was also recorded. Threshing was conducted using a stick of wood and a paddy thresher. Sorghum grains were packed in hermetic plastic bags. The conditions inside of the bags were  airtight and normal conditions. Each bag with different conditions inside of the bag was introduced with 10 pairs of Sitophilus zeamais (1-14 days old). Sorghum was stored for one, two and three months of storage under warehouse conditions. The results showed, that during storage the moisture contents of sorghum were  lower than that its standard safe moisture content (
POPULATION STRUCTURE OF Hoya spp. (APOCYNACEAE: ASCLEPIADOIDEAE) AT BODOGOL NATURE-CONSERVATION EDUCATION CENTER, INDONESIA Jessica Elfani Bermuli; Sulistijorini Sulistijorini; Sri Rahayu
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 26 No. 2 (2019)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.835 KB) | DOI: 10.11598/btb.2019.26.2.881

Abstract

Hoya species are a type of epiphytic flowering plants that are known to have traditional medicinal values. Since 2011, eight Hoya species are recorded at the Bodogol Nature-Conservation Education Center (BNCEC), Bogor, Indonesia. For effective conservation measures, this study is aimed to analyze the population structure and distribution pattern of Hoya species at BNCEC. Data collection was done from July to August 2016 using a  purposive sampling method in a 400m2 plot. The results show that each species differed in their population structures. The H. multiflora population structure has the shape of an inverted pyramid, where there were more adult individuals than seedling and young ones, both H. campanulata and H. imperialis have the shape of a natural population pyramid, showing a population balance between seedling, young, and adult individuals. H. lacunosa has the shape of an hourglass, in which the number of seedlings and adult individuals is larger than the young. H. hasseltii and H. vitellinoides have no clear population structures as no adult individuals were discovered for these two species. The distribution pattern of Hoya populations at BNCEC is of the clumped type (Morisita’s index = 0.661).
THE ESTABLISHMENT OF PROCECIDOCHARES CONNEXA IN WEST JAVA, INDONESIA : A BIOLOGICAL CONTROL AGENT OF CHROMOLAENA ODORATA SOEKISMAN THTROSEMITO
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 12 (1999)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.418 KB) | DOI: 10.11598/btb.1999.0.12.146

Abstract

Kirinyu (Chromolaena odorata (L.) R.M. King and H. Robinson) was reported for the first time in 1934 from Lubuk Pakam North Sumatera. It grows vegetatively during the wet season, flowers and sets a high number of fruits at the end of the wet season, and senesces during the dry season. It may be controlled manually by uprooting the weed or by slashing, and chemically by spraying with herbicides. However, this has not been successful. Recently a biological control agent, Procecidochares connexa was introduced at Parung Panjang, West Java, as a biological control agent of C. odorata. This paper reports the successful establishment of Procecidochares connexa. Two releases of P. connexa colonies were made in Parung Panjang at the end of 1995. The colony was able to survive through a harsh dry season of 1997. When C. odorata was swept by fire, the emerging shoots soon were attacked by P. connexa. The population of C. odorata went down to 37.2% with 31.8% of its emerging shoots attacked by P. connexa in a 2-year period. When twigs were attacked by  P. connexa,  the production of cypsellas was reduced by about 50% in one season. However, the survival of emerging flies was also affected by the death of twigs upon maturation of the cypsellas. Key words: Biological controUChromolaena odoratalProcecidochares connexa
SITE INDEX OF SIAMESE ROSEWOOD (Dalbergia cochinchinensis Pierre) IN PLANTATIONS OF THAILAND Taengmoo Phunchaisri; Chongrak Wachrinrat; Ponthep Meunpong; Suwan Tangmitcharoen; Nawaphong Kuasakun
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 27 No. 2 (2020)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (765.745 KB) | DOI: 10.11598/btb.2020.27.2.1199

Abstract

Currently, decreased productivity of Siamese rosewood (Dalbergia cochinchinensis Pierre) is a problem due to illegal logging and the guidelines to manage this prevention being may be insufficient. Forest plantations should be promoted to achieve sustainable wood production for this species. Site index (SI) is a measure that can be used to predict forest productivity to identify appropriate sites for planting Siamese rosewood. The objective of the current study was to predict the SI for Siamese rosewood in Thailand. A sample of 78 temporary plots at 26 sites in 16 provinces of Thailand was selected to construct a site index curve with a base age of 30 years. The results showed that the mean age of Siamese rosewood was 28 years (between 13 and 55 years), the mean diameter at breast height (DBH) was 20.03 cm (between 7.99 and 33.80 cm), and the mean dominant height was 20.79 m (between 7.32 and 24.82 m). The SI equation was: SI = e(lnHdo-12.476(A-1-Ab-1)) with an R2 value of 0.406 at p < 0.0001 (chosen as 30 years in the present study). The equation indicated that 14, 18, 22, 26, and 30 m trees belonged to site classes Ι, ΙΙ, ΙΙΙ, ΙV, and V, respectively. Although in natural forest, Siamese rosewood is distributed in dry evergreen and mixed deciduous forests, in the present study, several sites in areas under both in situ and ex situ conservation were in the good and very good site categories. Thus, both areas can be used to plant Siamese rosewood and increase its productivity.
MORPHOLOGICAL CHARACTERIZATION OF MALAYSIAN WILD BANANA MUSA ACUMINATA MUHAMMAD ASIF JAVED; MAK CHAI; ROFINA YASMIN OTHMAN
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 18 (2002)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.366 KB) | DOI: 10.11598/btb.2002.0.18.170

Abstract

Fourteen populations of Musa acuminata  ranging from populations in  the lowlands of northern (ssp. siamea)  to central Malaysian region  (ssp. malaccensis) and highland banana  (ssp. truncata) were characterized based on chromosome number and 46 morphological characters. A large amount of variation was observed within the populations. However, only highland bananas appeared morphologically distinct. Lowland populations both from northern and central Malaysia were found to be overlapping and no distinguishing pattern was observed. The morphological characters found variable within these populations were related to developmental changes and mutations. The results obtained in this study were not revolutionary. However, the survey of a large number of characters treated with multivariate techniques further sharpened the existing groupings of the Musa acuminata subspecies. Key words: Musaceae/ Malaysia / ssp. malaccensis I ssp. truncata I ssp. siamea
Biological Remediation of Cyanide: A Review Karamba Kabiru Ibrahim; Mohd Arif Syed; Mohd Yunus Shukor; Siti Aqlima Ahmad
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 22 No. 2 (2015)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1249.896 KB) | DOI: 10.11598/btb.2015.22.2.393

Abstract

Cyanide and its complexes are produced by industries all over the world as waste or effluents. Biodegradation is considered to be the cheapest and the most effective method to get rid of cyanide in the environment. Several studies on different types of microorganisms that can degrade cyanide in the environment have been carried out. Hydrolytic, oxidative, reductive, and substitutive/transfer reactions are some of the common pathways used by microorganisms in cyanide degradation. Biodegradation of cyanide can occur aerobically or an-aerobically depending on the environmental conditions. Immobilised enzymes or microorganisms prove to be very effective method of degradation. Microorganisms such as Klebsiella oxytoca, Corynebacterium nitrophilous, Brevibacterium nitrophilous, Bacillus spp., Pseudomonas spp. and Rhodococcus UKMP-5M have been reported to be very effective in biodegradation of cyanide.
LAND SUITABILITY ASSESSMENT FOR PATCHOULI (Pogostemon cablin) DEVELOPMENT AND ESSENTIAL OIL PRODUCTION Adi Setiawan
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 28 No. 1 (2021): BIOTROPIA Vol. 28 No. 1 April 2021
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.52 KB) | DOI: 10.11598/btb.0.0.0.1092

Abstract

The conformity of abiotic factors in the growing environment of crops determines the successful result of crop production. This study aims to assess and evaluate land suitability for plant growth and result of patchouli development in Dilem wilis, Bendungan Distric, Trenggalek Regency. The study was initiated with the survey and continued by utilizing analysis method to adjust the data with the criteria according to the classification of land suitability clashing and crops cultivation as indicator for assessing land suitability. The research was conducted on 3 locations from May to July 2017 for land suitability and from July to November 2017 for patchouli crops cultivation experiment. The result indicated from the three sampling Locations, specifically Location 1 presented the land suitability of N class, conforming that this Location was not suitable with the limiting factor of low K2O content of 0.08 me / 100 g. Meanwhile, both Location 2 and Location 3 presented similar land suitability class of S3s, tc, f, n conforming as less appropriate. The results of this study also depicted the influence of land suitability classes on plant growth; however, the difference in land classes (in this case S3 compared to N) did not demonstrate a correlation for oil yield and patchouli alcohol, where the element Potassium becomes a limiting factor.
CLONING OF A GENE ENCODING PROTEIN BELONGING TO ABC TRANSPORTER INVOLVED IN BACTERIAL MAGNETIC PARTICLE SYNTHESIS IN MAGNETOSPIRILLUM MAGNETICUM AMB-1 ARIS TRI WAHYUDI
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 16 No. 1 (2009)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (290.263 KB) | DOI: 10.11598/btb.2009.16.1.62

Abstract

Magnetospirillum magneticum AMB-1 synthesizes intracellular magnetic particles, magnetite (Fe3O4), enveloped by membrane called magnetosome under micro-aerobic conditions. Initial study of random transposon-based mutagenesis generated 62 nonmagnetic mutants of AMB-1 in a mini-Tn5 library. In order to identify a gene involved in bacterial magnetic particle (BMP) synthesis in the magnetic bacterium M. magneticum AMB-1, a nonmagnetic mutant from the library designated as NMA38-4, was analyzed. h e amino acid sequence deduced from the gene directly interrupted by transposon, ORF4 (1482 bp), showed homology to ATP binding cassette (ABC) transporter of Mesorhizobium loti with 62 % identity and 74 % similarity. It was strongly indicated by the occurrence of putative consensus sequence of ATP-binding motifs (ATP-binding protein). h e ORF4 was subsequently cloned in pET-15b and the recombinant ORF4-Histag fusion protein was heterologously expressed in Escherichia coli BL21 (DE3) pLysS. A 55 kDa protein corresponding to the ORF4-Histag fusion protein was obtained after purifi cation using Ni-NTA column. h is is the fi rst report describing a gene cluster containing gene encoding protein belonging to ABC transporter organized in an operon which is involved in BMP synthesis. Key words: Magnetospirillum magneticum AMB-1, Bacterial Magnetic Particle (BMP), ATP Binding Cassette (ABC) Transporter, transposon mutagenesis.

Page 4 of 45 | Total Record : 444


Filter by Year

1987 2023


Filter By Issues
All Issue Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023 Vol. 30 No. 1 (2023): BIOTROPIA Vol. 30 No. 1 April 2023 Vol. 29 No. 3 (2022): BIOTROPIA Vol. 29 No. 3 Desember 2022 Vol. 29 No. 2 (2022): BIOTROPIA Vol. 29 No. 2 Agustus 2022 Vol. 29 No. 1 (2022): BIOTROPIA Vol. 29 No. 1 April 2022 Vol. 28 No. 3 (2021): BIOTROPIA Vol. 28 No. 3 December 2021 Vol. 28 No. 2 (2021): BIOTROPIA Vol.28 No.2, Agustus 2021 Vol. 28 No. 1 (2021): BIOTROPIA Vol. 28 No. 1 April 2021 Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020 Vol. 27 No. 2 (2020) Vol. 27 No. 1 (2020) Vol. 26 No. 3 (2019) Vol. 26 No. 2 (2019) Vol. 26 No. 1 (2019) Vol. 25 No. 3 (2018) Vol. 25 No. 2 (2018) Vol. 25 No. 1 (2018) Vol. 24 No. 3 (2017) Vol. 24 No. 2 (2017) Vol. 24 No. 1 (2017) Vol. 23 No. 2 (2016) Vol. 23 No. 1 (2016) Vol. 22 No. 2 (2015) Vol. 22 No. 1 (2015) Vol. 21 No. 2 (2014) Vol. 21 No. 1 (2014) Vol. 20 No. 2 (2013) Vol. 20 No. 1 (2013) Vol. 19 No. 2 (2012) Vol. 19 No. 1 (2012) Vol. 18 No. 2 (2011) Vol. 18 No. 1 (2011) Vol. 17 No. 2 (2010) Vol. 17 No. 1 (2010) Vol. 16 No. 2 (2009) Vol. 16 No. 1 (2009) Vol. 15 No. 2 (2008) Vol. 15 No. 1 (2008) Vol. 14 No. 2 (2007) Vol. 14 No. 1 (2007) Vol. 13 No. 2 (2006) Vol. 13 No. 1 (2006) No. 25 (2005) No. 24 (2005) No. 23 (2004) No. 22 (2004) No. 21 (2003) No. 20 (2003) No. 19 (2002) No. 18 (2002) No. 17 (2001) No. 16 (2001) No. 15 (2000) No. 14 (1999) No. 13 (1999) No. 12 (1999) No. 11 (1998) No. 10 (1997) No. 9 (1996) No. 8 (1995) No. 7 (1994) No. 6 (1992) No. 5 (1992) No. 4 (1991) No. 3 (1989) No. 2 (1989) Vol. 1 No. 1 (1987) More Issue