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INDONESIA
eJurnal Kedokteran Indonesia
Published by Universitas Indonesia
ISSN : 23501426     EISSN : 23386037     DOI : 10.23886
Core Subject : Health,
Elektronik Journal Kedokteran Indonesia (eJKI) is a peer-reviewed and open access journal that prioritize the publication manuscript of students (medical education program, magister/specialist, doctoral). This journal publishes editorial, research article, reviews, evidence-based case report, and also interesting case reports/case study. eJKI published three times a year (April, August, December) and published by Faculty of Medicine Universitas Indonesia
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Articles 11 Documents
Search results for , issue "Vol 4, No 1 (2016): Vol 4, No. 1 (2016): April" : 11 Documents clear
Accuracy of Core Needle Biopsy for Musculoskeletal Lesion in Cipto Mangunkusumo Hospital Jakarta Peter Giarso; Achmad Fauzi Kamal; Nuryati Chairani Siregar
eJournal Kedokteran Indonesia Vol 4, No 1 (2016): Vol 4, No. 1 (2016): April
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Core needle biopsy (CNB) is considered to have similar results with open biopsy in accuracy and already becomes a routine procedure to establish the diagnosis of musculoskeletal lesion. However, the accuracy of CNB for muskuloskeletal lesion in Indonesia has not been reported. The aims of the study to know the accuracy of CNB for muskuloskeletal tumor diagnoses. From January 2011 to August 2015, all patients with musculoskeletal lesion in dr. Cipto Mangunkusumo Hospital underwent CNB and subsequently, tumour excision were indentified and enrolled. Diagnostic accuracy was evaluated for both histopathology and clinicopathological conference (CPC) conclusion. A total of 86 samples were enrolled in this study. The accuracy of CNB compared to post excision histopathology is 74.4%. With CPC conclusion, the accuracy is 83.7% with sensitivity 98%, specificity 59% (p<0.001). The accuracy of CNB after immunohistochemistry was increased from 74.4% to 84.9% with sensitivity 98%. The accuracy of CNB was 97.1% and 82.7% for benign lesion and malignant lesion respectively (p<0.01). The accuracy of CNB to differ and to confirm diagnosis as primary or metastatic lesion was 97.2% and 85.7% respectively (p<0.001). Inconclusion, CNB is a reliable diagnostic method to establish musculoskeletal tumor diagnoses. CPC significantly provides better accuracy rate of CNB.
Comparison of Antioxidant Activity and Tannin Level of Pegagan Extract to Commercially Available Product Achmad Zaki Maulidzy; Adisti Dwijayanti
eJournal Kedokteran Indonesia Vol 4, No 1 (2016): Vol 4, No. 1 (2016): April
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Centella asiatica (pegagan) is a well-known traditional medicinal plant that has been processed into many commercial herbal products. Those herbal products were claimed to have antioxidant effects which were beneficial to overcome free radicals in the body. Antioxidant activity of those commercial products compared to the extracts has not been studied yet. The aim of this study is to compare the antioxidant activity and tannin levels of water and ethanol extracts of C.asiatica compared to three herbal products of C.asiatica. Water extract is made by infusion while ethanol extract is made by maceration. Three brands of C.asiatica products were obtained from the market. Levels of antioxidants were measured by comparing the EC50 value using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Tannin levels were measured qualitatively and semiquantitatively using FeCl3. Results showed that antioxidant activity of the water and ethanol extract of C.asiatica wer significantly higher than the commercial products (p&le;0.05). Tannin&rsquo;s semiquantitative test showed that all samples contained tannins but the amount was very low.
Effect of Pomegranate’s Extract to Malondialdehyde Level and Rat’s Heart Vessel Feri Fadillah; Fransisca Tjakrawidjaja
eJournal Kedokteran Indonesia Vol 4, No 1 (2016): Vol 4, No. 1 (2016): April
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Pomegranate (Punica granatum) contains high level of antioxidant. The aim of this study is to evaluate the effect of pomegranate extract on MDA level and histopatological findings in rat&rsquo;s heart vessel that is exposed to cigarette smoking. Experimental study included sprague dawley rats that were exposed to cigarette smoke 2-3 hours a day for 2 weeks. The rats were divided into 3 groups: the first group was a control group, the second group received 5% pomegranate extract, and the third group was given 10% pomegranate extract. Measurement of MDA levels was done before and after the intervention (at the 8th day and 15th day). The MDA levels were significantly higher in control group than those among intervention group (p<0,05). Histopathological finding showed damage of endothelial cell and microhemorrhagic in rat&rsquo;s heart vessel due to tissue hypoxia.
Effect of 70% Ethanol Extract Ointment of Karamunting Leaves on Re-epitelisation Incision Wound Healing on Wistar Rat’s Skin Wenny Rupina; Heru Fajar Trianto; Iit Fitrianingrum
eJournal Kedokteran Indonesia Vol 4, No 1 (2016): Vol 4, No. 1 (2016): April
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Recent researches indicate that traditional plant may act as a wound healing agent, one of them is Karamunting leaves. The aim of this research is to find out the effect of 70% ethanol extract ointment of karamunting leaves on re-epithelialization of incision wound healing on wistar rat&rsquo;s skin. This research was an experimental research with post-test only control group design. The research subjects were twenty male wistar rats in which further divided to five groups; positive control group (sanoskin&reg; ointment), negative control group (placebo ointment), experimental group 1 (2,5% karamunting leaves ointment), experimental group 2 (5% karamunting leaves ointment) and experimental group 3 (10% karamunting leaves ointment). All groups underwent 2 cm long incision at the back with 0,2 cm depth, parallel with os.vertebrae, within 5 cm from the ear.On the tenth day, wound tissue were taken from all groups sample and processed into histological blood smears stained with H&E to check the thickness and the width of the slit skin epithelium observed with 10x objective lens magnification. The 70% ethanol extract ointment of leaves Karamunting decreased slit thickness epithelium (p<0,05) and minimized slit width epithelium (p<0.05) compared to negative control. 70% ethanol extract ointment of leaves Karamunting increased wound re-epithelialization of incision wound on wistar rat&rsquo;s skin with an effective concentration of 5%.
Expression of NFkB in Locally Advanced Cervical Cancer and Its Relation to Radiotherapy Response Affi A. Ratnasari; Hariyono Winarto; Sigit Purbadi; Sri M. Sekarutami; Bambang Sutrisna
eJournal Kedokteran Indonesia Vol 4, No 1 (2016): Vol 4, No. 1 (2016): April
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

One of the expected elimination pathways of cancer cells in the process of radiation is apoptosis. Nuclear factor kappa-B (NFkB) is a transcription factor related to the mechanism of apoptosis resistance of a cell, which can affect the post-radiation response. The aim of this study is to assess the relative expression of NFkB in locally advanced cervical cancer and its relation to radiotherapy response. The study was conducted with observational cohort design on January-October 2015. Pre-radiotherapy cervical biopsy specimen was collected from 17 subjects, while only 10 subjects were willing to be taken mid-radiotherapy cervical biopsy specimen. We extracted and analyzed mRNA of NFkB by using qRT-PCR. NFkB relative expression was tested to its correlation with radiotherapy response. This study demonstrated that median of pra-radiotherapy relative expression on subjects with negative response (0.667; SD 0.413) was higher than positive response (0.315; SD 160.298), but not statistically significant (p=0.578). Alteration of mid-radiotherapy NFkB relative expression compared to pra-radiotherapy showed correlation to radiotherapy response. It is concluded that reduced expression of mid-radiotherapy NFkB relative expression plays a role in increasing radiotherapy response.
Polymorphism of Human FcγRIIa and Its Association with P. falciparum Density and Efficacy of Dihydroartemisinin- Piperaquine Sylvia Sance Marantina; Din Syafruddin; Heri Wibowo; Inge Sutanto; Puji Budi Setia Asih; Suradi Suradi; Ismail E Prayitno
eJournal Kedokteran Indonesia Vol 4, No 1 (2016): Vol 4, No. 1 (2016): April
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Fc&gamma;Rlla dimorphism has been related to the ability of the host to eliminate malaria parasite so it is necessary to investigate the allele polymorphism Fc&gamma;Rlla of population in malaria-endemic areas in Indonesia in order to know the role of immunity in eliminating malaria parasite. A total of 120 samples of Dried Blood Spot (DBS) falciparum malaria acquired from DHP drug efficacy studies in 5 regions in Indonesia were analyzed by Polymerase Chain Reaction (PCR) and sequencing, to look at variants of Fc&gamma;RIIa-131 allele and its Association with Parasite DensityandEfficacy ofDihydroartemisinin- Piperaquine. The Fc&gamma;RIIa gene analysis indicated that genotype RH has the highest frequency (50.8%) compared to RR (17.5%) and HH (31.7%). Allele R131 showed a protective effect against High Density Parasitemia (HDP) (>5000 parasites/&mu;L; odds ratio [OR]= 0.133, 95% confidence interval [CI]= 0.053&ndash;0.334, P< 0.001) and associated with longer gametocytes carrier clearance time (> 72 hours; Relative Risk [RR]= 1,571, 95% confidence interval [CI]= 1,005&ndash;2,456, P= 0.090).
Effect of Temperature towards RNA Concentration: Quantitative Investigation with Spectrophotometer Feby Fariska Savira; Novi Silvia Hardiany
eJournal Kedokteran Indonesia Vol 4, No 1 (2016): Vol 4, No. 1 (2016): April
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Ribonucleic acid (RNA) is a thermodynamically unstable molecule. The way RNA samples are preserved is critical to maintain maximum yield and quality, therefore it is useful for molecular analysis such as real time- PCR. There are many contradictions and variations regarding the temperature for RNA storage. The aim of this study is to find the ideal temperature to store RNA among -80o C, -20o C and 4oC by determining changes in RNA concentration over two weeks of storage time. The liver of eight rats was divided into three groups, weighing from 25-26 &mu;g. Samples were homogenized, isolated and stored in -80oC, -20oC and 4oC freezers. Absorbance was measured with spectrophotometry at 260 and 280 nm to determine the concentration of RNA. There was no significant difference in the concentration of RNA samples stored in all temperatures after two weeks, both experimentally and statistically (Kruskal-Wallis, -80oC p=0.949; -20oC p=0.885; 4oC p=0.935). In conclusion, RNA can be stored in -80oC, -20oC and 4oC for two weeks without quantity reduction. Longer duration of study and RNA quality analysis is recommended to check for RNA degradation.
Detection of Cryptosporidium sp Coproantigen in Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome Patient with Chronic Diarrhea Sri Wahdini; Agnes Kurniawan; Evy Yunihastuti
eJournal Kedokteran Indonesia Vol 4, No 1 (2016): Vol 4, No. 1 (2016): April
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Cryptosporidium sp is one of protozoan that cause diarrhea in immunodeficient patients such as HIV/AIDS. Detection of coproantigen of Cryptosporidium sp is more sensitive than microscopic detection. The objective of this study is to detect cryptosporidiosis using coproantigen detection compare to microscopic detection from stool of HIV/AIDS patients with crhonic diarrhea. A Total of 95 stool specimens from HIV/AIDS patients with chronic diarrhea were received by Laboratorium klinik Parasitologi Faculty of Medicine UI. The stool was tested using coproantigen and microscopy detection for cryptosporidiosis. The frequency of cryptosporidiosis using koproantigen detection was 36.8% while the MTA method was only 11.6%. Sensitivity and specificity of coproantigen detection compared with the microscopic was 100% and 71.4%. Detection coproantigen necessary in patients with high suspicion of cryptosporidiosis but oocysts detection was negative.
Case Management of Pure Gonadal Dysgenesis 46, XY (Sindrom Swyer) Kanadi Sumapradja; Mila Maidarti; Achmad K. Harzif; Budi Wiweko; Gita Pratama; Muharam Natadisastra; Andon Hestiantoro
eJournal Kedokteran Indonesia Vol 4, No 1 (2016): Vol 4, No. 1 (2016): April
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Disorders of sex development (DSD) are medical conditions in which the development of chromosomal, gonadal or anatomic sex varies from normal and may be incongruent with each other. Swyer syndrome is a condition caused by pure gonadal dysgenesis 46,XY, which followed by inadequate anti-mullerian (AMH) production results in maintenance and further development of mullerian duct into uterus. Therefore, many patients who suffer from this condition initially come with chief complaint of primary amenorrhea with insufficient development of secondary sexual characteristics. Patient&rsquo;s gender orientation commonly is a female, since her brain was not sufficiently exposed with androgen hormone. Management of DSD patients should be based on patient-centered approach. Therefore, overall management of DSD patients should follow according to patient&rsquo;s perception regarding to her gender orientation. Herein, we reported a case of Swyer syndrome in female aged 29 years with chief complain primary amenorrhea. Chromosomal analysis was 46,XY (20 mataphase), FSH level was 31.5miu/ml, LH 10.8miu/ml, estradiol (E2) <5pg/ml, testosteron total (T) <0.0025ng/ml. Medical management for this patient has the purpose to drive her feminization process in order to improve her physical appearance. However, since there is a great tisk for having gonadal tumor development from intra-abdominal dysgenetic gonad with Y chromosome, this patient has been strongly suggested to have bilateral gonadectomy.
Diagnosis and Treatment of Uveitis to Prevent Blindness Ratna Sitompul
eJournal Kedokteran Indonesia Vol 4, No 1 (2016): Vol 4, No. 1 (2016): April
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Uveitis is an inflammation of the uvea which may result in blindness. Uveitis may be caused by limited inflamation of the uveal tract, manifestation of systemic diseases (autoimmune, infection, cancer), expansion of inflammation in the cornea and sclera, trauma or idiopathic. Anterior uveitis is an inflammation of the iris and cilliary body with symptoms of pain, red eye, photophobia, and decrease in visual acuity. Intermediate uveitis is the inflammation of the pars plana and frequently involves anterior vitreous and posterior uveitis. Clinical manifestation of intermediate uveitis is usually mild without red eye and pain, however vision may decrease due to macular edema and cell aggregation in vitreous. Posterior uveitis is an inflammation involving choroid layer, which is common in developing countries due to high prevalence of infectious diseases (toxoplasmosis, tuberculosis, HIV, syphilis). Patient may complain of blurry vision but not accompanied by pain, red eye, and photophobia. Complications of posterior uveitis are cataract, glaucoma, macular edema, keratopathy, turbidity of vitreous, retinal detachment, and optic nerve atrophy. The prognosis of posterior uveitis is worse than anterior uveitis. Panuveitis is an inflammation of the uvea and surrounding structures (retina, vitreous). Diagnosis is made based on anamnesis, ophthalmic examination, laboratory examination, and imaging. Treatment of uveitis is intended to reduce inflammation, minimize structural destruction, prevent blindness, reduce pain and photophobia. Corticosteroid and immunosuppresant are the drugs of choice to manage the inflammation, where NSAID is used to reduce pain and cyclopegic administration to prevent posterior synechiae. Antimicrobial is given if uveitis is caused by infection. Underlying diseases of uveitis must be treated comprehensively to prevent further progression, complications and blindness.

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