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Isna Rasdianah Aziz
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Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar. Jl. H.M. Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia. 92113
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Biogenesis: Jurnal Ilmiah Biologi
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23021616     EISSN : 25802909     DOI : https://doi.org/10.24252/bio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic publication for Islamic scientists, which could contribute to the advancement of tropical biology studies in the Islamic world. The journal invites research articles, short communication, critical and comprehensive scientific reviews concerning tropical biosciences in Asia, specifically in the following subjects: Islamic-integrated tropical bioscience Tropical biodiversity and conservation Biosystematics of tropical species Biochemistry and physiology of tropical species Ecological study and behavior of tropical species Tropical genetics, biotechnology, and bioinformatics
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 285 Documents
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A modified high-fat diet and its effect on histopathological features of mice liver as an alternative diet for animal model of liver cell damage Dzul Fithria Mumtazah; Hendri Busman; Mohammad Kanedi; Gina Dania Pratami
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.24081

Abstract

Researchers attempted to obtain animal models that experienced hypercholesterolemia and led to liver damage to determine the performance of the liver. Rats were chosen because of the ease of handling, collecting organ and blood samples. Still, their high price and availability that are more difficult to find have made some researchers look for alternatives to other animal models such as mice. This study aims to determine the role of a modified high-fat diet as an alternative diet for mice to experience hepar damage. Male mice treated for four and eight weeks with a modified high-fat diet were sacrificed for their liver, then they were tested for histopathology using the paraffin method and HE staining. The characterization of hepar damage traits was carried out to score the degree of parenchymal degeneration, hydropic degeneration, and necrosis. The results showed that the control group, four and eight weeks of atherogenic diet had more than 50% cell damage, presumably due to the role of the starch mixture in feed as a source of carbohydrates through the mechanism of converting carbohydrate pathways into fat which damages liver cells.
Reef fish diversity in the Legon Bajak Port, Kemujan Island, Karimunjawa National Park Siti Lathifah Maulany; Ardyan Pramudya Kurniawan
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.24091

Abstract

Karimunjawa National Park provides substantial reef fish resources, leading to higher market demand for reef fish production. This study aims to determine the diversity of reef fish, particularly in regions where fishing boats dock, specifically in Legon Bajak Port, Kemujan Island, Karimunjawa National Park, Jepara, Central Java. The findings of this investigation should enrich previous studies. The study was conducted on the right and left sides of the Legon Bajak Port. The visual count of belt transects was used to obtain data on reef fish. The study results obtained the number of reef fish at the Legon Bajak Port was 2769 individuals/500 m2 on the right and 1809 individuals/500 m2 on the left side, representing 40 species, 26 genera, and 13 families. Istigobius rigilius had the largest density of individuals, with 233 individuals/500 m2. The fish diversity index (H') value on the right side of the Legon Bajak Port was 3.24, which includes the high category, and on the left side was 2.89, which includes the medium category. The dominance index (D) was between 0.05 and 0.06 (low), while the uniformity index (E) was between 0.74 and 0.83 (unstable in a stable community).
Analysis of genetic diversity of bacteria Xanthomonas oryzae pv. oryzae causes leaf blight in North Sumatra Zulheri Noer; Maimunah Maimunah; Erwin Pane; Eko Prasetya
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.24092

Abstract

Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight on rice plants, which is responsible for crop failure, resulting in economic losses. Control of Xoo bacteria can be accomplished through the use of Xoo-resistant rice varieties. Due to the shifting genetic structure and environmental conditions of Xoo bacteria, it is difficult to identify them according to their pathotype. This study aims to determine the genetic diversity of Xoo bacteria using the polymerase chain reaction (PCR) method and the IS1112-based RAPD marker, which is known to contain repetitive sequences. DNA was isolated from 52 isolates collected from 15 districts and cities in North Sumatra province and then used to amplify the IS1112 sequence. Dendrogram analysis revealed that cluster analysis of PCR findings classified isolates into 15 groups with a 90% similarity value. Genetic variation among Xoo isolates from North Sumatra ranged between 51% and 100%. North Sumatra Xoo isolates exhibited a high degree of genetic diversity. This findings can be used as a resource for future management of the Xoo bacteria by expediting disease-resistant rice breeding projects in various rice producing countries.
Efficacy of knockdown insecticide based on Permot (Passiflora foetida L.) leaf extract against mortality of German cockroach (Blattella germanica L.) Rina Priastini Susilowati; Flora Rumiati
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.24100

Abstract

Chemical insecticides have a detrimental effect on not only the target species, but also on non-target species and the environment. Transfluthrin is a pyrethroid class II insecticide that is considered to be safe for humans. However, repeated use over an extended period of time can result in cockroach resistance. For this reason, this study sought out alternative materials, specifically Permot leaf (Passiflora foetida L.). This study aims to determine the neurotoxic efficacy of insecticides derived from Permot leaf extract on German cockroach knockdown time and death. A total of 120 German cockroaches and was replicated three times. After 1 h of exposure to insecticides containing transfluthrin 3000 ppm and Permot leaf extract up to a dose of 4000 ppm, knockdown time and cockroach death were observed. The probit test was used to determine the cockroach knockdown time. The study's findings indicated that when cockroaches were exposed to insecticides derived from Permot leaves at a dose of 4000 ppm, the knockdown efficacy was KT50 for 8 min and KT90 for 30 min. The blocking test on German cockroach spiracles established that exposure via cockroach spiracles was more effective than exposure via other cockroach organs in killing.
Tillering response of liquid organic fertilizer and monosodium glutamate supplementation on Ganyong (Canna discolor Lindl.) Mahdalina Mursilati; Agus Suprapto; Esna Dilli Novianto
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.24196

Abstract

Ganyong (Canna discolor) is a plant that contains a high carbohydrate content, utilizing as alternative food for rice, especially in Indonesia. One of the factors affecting growth is the quantity of tillers, which affect the productivity of Ganyong's tuber. The purpose of this study was to discover the optimal combination of liquid organic fertilizer and monosodium glutamate to enhance the number of tillers. This study employed a randomized complete block design with a two-factor structure. The first factor to consider was the concentration of liquid organic fertilizer, which was specified as 0 ml/L, 2 ml/L, 3 ml/L, and 4 ml/L. The second factor was the monosodium glutamate dosage, which ranged from 0 to 3 g/plant, 6 g/plant, and 9 g/plant. Ganyong were cultivated for six months on farmland at an elevation of 460 m asl. Supplementation with liquid organic fertilizer resulted in a significant increase in the number of tillers. While monosodium glutamate supplementation had no significant effect on the formation of tillers. Combining liquid organic fertilizer at a concentration of 3 ml/L with monosodium glutamate 6 g/plant resulted in the optimal number of tillers.
Comparising DNA extraction from environmental DNA samples to reveal the diversity of freshwater metazoans Donan Satria Yudha; Dwi Sendi Priyono; Rahma Izzati; Aulia Sigit Ardianto; Ananto Puradi; Nainggolan Nainggolan
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.24390

Abstract

Environmental DNA (eDNA) monitoring has gained popularity in the last decade as one of the most sensitive and cost-effective monitoring methods. However, information regarding the type of DNA extraction used still needs to be studied, especially for metazoan in fresh water samples. This parameter is also critical for a project's experimental design. This study aims to compare the effectiveness of two extraction kits between DNeasy® Blood & Tissue Kit (Qiagen) silica column-based and ZymoBIOMICS 96 MagBead DNA Kit (Zymo Research) magnetic bead-based. The quantity of DNA extracts was measured using a spectrophotometer at 260/280 nm. Following that, we continued the metazoa PCR procedure. Qiagen has higher mean value of DNA concentration (88.48 ng/μl) than Zymo (20.89 ng/μl). For DNA purity, Zymo has higher mean value of DNA purity (1.84) than the Qiagen (1.59). However, both kits were equally successful in amplifying universal metazoan primers. We recommend that the use of these types of kits appears to be the least important consideration. Other important factors that may have a major impact on DNA extraction such as water volume, membrane type, sampling strategy need to be investigated in freshwater samples.
Prediction of SARS-CoV-2 3C-like protease (3CLpro) crystal structure to provide COVID-19 inhibitor design through computational studies Taufik Muhammad Fakih; Dwi Syah Fitra Ramadhan
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.24520

Abstract

Infectious diseases have lately become pandemic, posing a threat to global public health with the introduction of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), previously provisionally named 2019 novel coronavirus or 2019-nCoV).  Technological advancements have increased the possibility of discovering natural inhibitor candidates capable of preventing and controlling COVID-19 infections. The SARS-CoV-2 3C-like protease (3CLpro) is critical for SARS-CoV-2 replication and is a prospective therapeutic target. This study aims to identify, evaluate, and explore the 3CLpro macromolecular structures from SARS-CoV and SARS-CoV-2, as well as their impact on angiotensin-converting enzyme 2 (ACE-2). The discovery of the two 3CLpro macromolecules revealed structural similarities in several regions. These findings were subsequently confirmed by performing protein-protein docking simulations to observe the interaction of 3CLpro with the active site ACE-2. With an ACE score of 701.41 kJ/mol, SARS-COV-2 3CLpro forms the strongest binding with ACE-2. As a result, the findings of this research can be used to guide the development of potential SARS-CoV-2 3CLpro inhibitors for the treatment of COVID-19 infectious diseases.
Evaluation of antimicrobial activity and phytochemical screening of red Kamboja (Plumeria rubra L.) extracts Ni Kadek Yunita Sari; Ni Wayan Deswiniyanti; Putu Angga Wiradana
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.25409

Abstract

Natural antimicrobial sources such as red Kamboja (Plumeria rubra L.) flower extract can be utilized to treat infectious disorders caused by Staphylococcus aureus, Escherichia coli, and Candida albicans. This study aims to determine evaluate the effectiveness of methanol and ethanol extracts of P. rubra floral against E. coli, S. aureus, and C. albicans growth, as well as the amount of secondary metabolites in P. rubra extract. The study's findings indicate that the highest DIZ value of P. rubra methanol extract was 7.40 mm, 7.36 mm, and 7.30 mm for S. aureus ATCC25923 at 5%, 10%, and 20%, respectively, while the highest DIZ value for C. albicans ATCC10231 at 10%, 10%, and 20% was 25.08 mm, and 25.04 mm, respectively. The DIZ value of the P. rubra flower ethanol extract against E. coli strain was 5.26 mm at 5%, and 7.30 mm at 20%. Secondary metabolites of saponins, tannins, alkaloids, flavonoids, steroids, and phenols were present in the methanol and ethanol extracts of P. rubra flowers. In summary, our findings highlight the use of P. rubra flower extract as a biological source with antibacterial properties for the control of human infectious illnesses.
Effects of soaking time and freezing on the gel strength of Moi-Moi prepared from Cowpea grains Ikechukwu Otuosorochi Amagwula; Chijioke M. Osuji; Chinenyenwa Gloria Omeire; Umama Yezdani; Mohammad Gayoor Khan; Chinaza Godswill Awuchi
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 1 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i1.19599

Abstract

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Atlas moth (Attacus atlas Linnaeus, 1758) silk sericin protein: The effect of extraction methods and storage time on its content Hipny Alwandri; Sukirno Sukirno; Siti Sumarmi
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 1 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i1.24373

Abstract

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