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Nexus Kedokteran Translasional
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Articles 123 Documents
Molecular Docking Analysis of Family Fabaceae Phytochemistry to Estrogen Receptor Alpha Compared to Tamoxifen in Breast Cancer Putra, Ega Caesaria Pratama; Pesik, Riza Novierta; Kusumawati, Ratna
Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Breast cancer is the second most common cancer.Estrogen is the risk factor of breast cancer. Estrogen receptor α is expressed by 50-80% of breast cancer.  The mechanism of estrogen is mediated by estrogen receptor α and estrogen receptor β. Tamoxifen is a selective estrogen receptor modulators (SERMs) that can be bound to the estrogen receptor α, therefore prevent bonding between estrogen and estrogen receptor α. Methods: This research used molecular docking analysis of family Fabaceae’s Phytochemistry as an inhibitor of estrogen receptor α activation. This study was observational bioinformatics study to observe interaction between family Fabaceae’s phytochemistry and estrogen receptorα. Molecular docking analysis observed binding energy and binding location. This virtual screening analysis was done using PyRx, AutoDock Vina, PyMOL, Open Babel, and UCSF Chimera. Results: We obtained 471 Family Fabaceae’s phytochemistry from database, only five compounds that have equal or lower binding energy compared with tamoxifen, that compound werebeta-amyrine (9.6 KJ/Mol), obovatin( 9.6 KJ/Mol), erythrabyssin II (9.6 KJ/Mol), Cajaflavanone (10.2 KJ/Mol), and tomentosanol E (10.5 KJ/Mol). The visualization of binding location analysis showed that only cajaflavanone which have relatively similar binding site location with tamoxifen. Conclusions: Cajaflavanone have a similar characteristic with tamoxifen, and have a potency to be used as partial antagonist of estrogen receptor alpha in breast cancer based molecular docking analysis. However, In vitro and In vivo researchneeded to determine the effectiveness. Keywords: Breast Cancer, Molecular Docking, Fabacea, Tamoxifen, Estrogen Receptor α 
Effect of Ethanolic Extract of Centella asiatica against Cortex Hypertrophy of Adrenal Gland Induced by Chronic Immobilization Stress in Rats Hidayat, Achmad Nurul; Wasita,dr.,Ph.D, Brian -; Wiyono,dr.,M.Kes, Nanang -
Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Chronic stress affects the cortex changes such as hypertrophy which is caused by the physiology change of HPA (Hypothalamus-Pituitary-Adrenal) pathway. Pegagan extract contains beneficial compounds such as triterpenoid is known has antistress and antidepressant effect. This present study aims to investigate Ethanolic Extract of Centella asiatica (EEC) on cortex hyperthropy level induced by chronic immobiliztion stress in male rats. Methods: This study was laboratory experimental study with randomized post test only control group design. Paraffin blocks was processed from thirty Sprague Dawley male rats. They were previously subjected with chronic immobilization stress and then divided into 6 groups: control group given distilled water 1 mg/kg, stress + PGA (Pulvis Gummi Arabicum), stress + 150 mg/kg EEC, stress + 300 mg/kg EEC, stress + 600 mg/kg EEC and stress + fluoxetine 10 mg/kg. The treatments were performed for 21 consecutive days. Cortex hypertrophy was evaluated using ImageJ 1.48v software under light microscope from hematoxylin eosin staining. Statistic analysis was performed using Kruskall-Wallis test continued by Post Hoc Mann-Whitney Test with Bonferroni correction. Result: Kruskall-Wallis test result showed significant differences in six group with p= 0,007. Meanwhile, Mann Whitney test with Bonferroni correction did not show significant difference between stress + PGA group and other groups (p>0.05). The difference result only found between control group and stress + 300 mg/kg of EEC (p= 0,001). There were no significant difference between stress + fluoxetine 10 mg/kg and control group (p = 0,880). Conclusion: EEC was incapable to decrease cortex hypertrophy level of adrenal gland in Sprague Dawley male rats induced by chronic immobilization stress. Keywords: Centella asiatica, chronic immobilization stress, hypertrophy, adrenal glands. 
Antibacterial Activity Test of Aloe vera L. Leaf Extracts on Extended Spectrum β-Lactamase (ESBL) Producing Bacteria from Surgical Site Infection Isolate Zakiatunnisa, .; Maryani, .; Setyawan, Sigit
Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Surgical site infection is clinically defined as a discharge of purulent around wounds or cellulitis inside postoperative wounds. One of the factors that cause surgical site infections is the ESBL producing bacteria that developed a resistance towards most antibiotics. Aloe vera L. is known to possess a lot of antibacterials potent against various bacteria, either gram-positive or gram-negative. This research aims to prove the effects of the administration of Aloe vera L. leaf extracts towards  the growth of ESBL producing bacteria from surgical site infection isolates. Method of research: This research was an experimental laboratory  research (post test only with control group design) with sampling technique non-probability sampling ;  consecutive sampling. The research was done in the UNS Faculty of Medicines Laboratory of Microbiology. Extraction of samples was done in the Dr. Moewardi Public Hospital Laboratory of Microbiology.The independent variable of this research was 70% ethanol extracts of Aloe vera L. leaf with concentrations of  25%, 50%, 75%, and 100%. The research subject was surgical site infection isolate ESBL producing bacteria, standardized with Mc Farland 0.5. Sensitivity test on the Muller-Hinton medium used the diffusion method with cefoperazon/sulbactam as positive control and aquades as negative control. The result of research was tested with Kruskal-Wallis­ testing method followed by Mann-Whitney testing method. Results: Kruskal-Wallis test results indicates a significant interception potential between two treatment groups (P = 0.000). Mann-Whitney test results indicates a significant difference of interception potentials on every treatment group, with the exception of the interception potential groups of 75% concentration extract and 100% concentration extract, no significant interception potential was found (P = 0.179). Conclusion: Aloe vera L. leaf extract proves to possess potential activity interception towards the growth of ESBL producing bacteria from surgical site infection isolates. The concentrations of Aloe vera L. leaf extract possess a positive correlation towards the interception potentials on surgical site infection ESBL producing bacteria. Keywords: Infection, postoperative wound, ESBL, Aloe vera.  
Molecular Docking Analysis of Estrogen Receptor α to Phytochemistries in Asteraceae Family Compared to Tamoxifen in Breast Cancer Umam, Dzulfiar Nasir; Pesik, Riza Novierta; Kusumawati, Ratna
Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background:Tamoxifen is the first line hormonal therapy for Estrogen Receptor α(ERα) breast cancer. Some of phytochemistries pose anticancer activities. However, data of those from Asteraceae family in Indonesia are lacking. The aim of this research is to know the binding affinities and binding locations of phytochemistries in Asteraceae family from Indonesia compared to tamoxifen on ERα in breast cancer. Methods: This research wasa bioinformatic study. Subjects were3-dimensional structures ofERα, tamoxifen, and phytochemistries’s Asteraceae family obtained from Indonesian HerbalDB database, Pubchem Compound, and Protein Data Bank. Subjects were prepared by Chimera 1.10 and Open Babel. Tamoxifen was docked on ERα by AutoDock Vina to know its binding affinities of ligand-receptor complex, compared to the other chosen ligands. Binding locations of ligand-reseptor were visualized by Pymol 1.7.2. Results: The docking results showed four phytochemistries’s binding affinities whichwere stronger than tamoxifen (-9.6 kcal/mol), such as lappadilactone, friedelin, benperidol, and beta-amyrin. The visualization results showed that lappadilactone, friedelin, benperidol, beta-amyrin, taraxerol, andepifriedelanol had similar binding areas totamoxifen on ERα. Lappadilactone had hydrogen bond with Tyr526 and benperidol had hydrogen bond with Trp383 on ERα. Conclusions:Lappadilactone and benperidol have stronger binding affinities than tamoxifen and they have hydrogen bond with ERα, but not in active sites of ERα. Therefore, lappadilactone and benperidol may have ability to inhibit ERα activities computationally. Keywords: Estrogen receptor α, molecular docking, phytocemistry, tamoxifen.
Identification of Herbal Compounds as Thymidylate Synthase Inhibitors that more Potent than 5-Fluorouracil using Molecular Docking Pratama, Yoga Mulia; Indarto, Dono; Suselo, Yuliana Heri
Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Development of new selective anticancer drug is an important necessary. Because of that, some effective way to screen herbal compounds which have good potency for developing drug is needed, one of them is using molecular docking methods. In this study, researchers identify herbal compounds as new thymidylate synthase (TS) inhibitors which more potent than 5-Fluorouracil (5-FU) using molecular docking. Methods: Three-dimensional structure samples of herbal compounds from Indonesian herbal database (herbaldb.ui.ac.id) were docked with three-dimensional structure of TS protein using iGEMDOCK program. Then, the compounds which have lower docking score than fdUMP, as standard ligand, clustered using ChemMine. Results: The docking result showed that 27 herbal compounds have docking score lower than fdUMP in iGEMDOCK program. The 27 compounds have higher affinity to fdUMP and it can form a stable complex with TS protein. Protein-ligand complexes showed that all of 27 compounds have same binding site with fdUMP. But folic acid is excluded from the result because it is a TS ligand. fdUMP has similar structure with UMP, a TS ligand, but folic acid which has lowest docking score is not similar with UMP. Conclusions: The 26 compounds, except folic acid, are more potent than 5-FU as TS inhibitors by docking study. Further study to delineate the effectivity of molecular docking is needed. Keywords: 5-Fluorouracil, herbal compound, molecular docking, thymidylate synthase
The Difference of Efficacy Between Granule Form of Pandan Wangi Leaf (Pandanus amaryllifolius Roxb.) Extract and Abate Against Aedes aegypti L. Khotimah, Dewi Nur; Handayani, Sutartinah Sri; Haryati, Sri
Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Abatisasi is considered quite effective against Aedes aegypti L. as a dengue hemorrhaegic fever vector. Improperly use of abate dose can interfere human health and prolonged use can cause larvae resistance so that need an alternative larvicide. Granule of pandan wangi leaf extract (Pandanus amaryllifolius Roxb.) has a potention to be an alternative larvicide. This study aims to determine the effectiveness differences between pandan wangi leaf extract granule with abate against Aedes aegypti L. Methods: This study uses a quasi-experimental method  with the approach posttest only control group design. This study was conducted in September and October 2014 in B2P2VRP Salatiga using the inclusion and exclusion criteria have been determined. The sampling technique used is incidental sampling. The independent variable is abate and pandan wangi leaf extract granule, while the dependent variable is the number of Aedes aegypti L. larvae instar III that be dead. Researcher compare into two different groups, namely pandan wangi leaf extract granule with various concentrations and abate. The data analysis used is One Way ANOVA continuing with LSD test and Probit Analysis. Results: The effectiveness of pandan wangi leaf extract granule is more lower than abate. The result of One Way ANOVA test shows a significance value p <0:01 (p <0.05), which means that there is a significant difference in mortality between the treatment group of larvae and the differences is statistically significant. The result of LSD test shows that abate 1% is significantly different with pandan wangi leaf extract granule 1.5%, 3.5% and 8%, while abate 1% is not significantly different with pandan wangi leaf extract granule 12.5% and 17%. The result of Probit Analysis shows LC50 is 7.007% and LC99 is 94,745%. Conclusions: There is an effectiveness difference between pandan wangi leaf extract granule with abate to against Aedes ageypti L. larvae. Keywords: Aedes aegypti L., pandan wangi leaf extract granule, abate. 
Screening Indonesian Medical Plants Phytochemistry Using Molecular Docking as Hepcidin Antagonis in Iron Deficiency Anemia Adiparadana, Dien; Suselo, Yuliana Heri; ., Balgis
Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Iron deficiency anemia is found in clinical practice and community. There is mutasion in a genetic within iron deficency anemia which regulate iron homeostatis.Mutation in the TMPRSS6 resulting in iron refractory iron deficiency anemia  The TMPRSS6 genetic encoding matriptase-2 enzymes which regulate hepcidin. The hepcidin is a principal regulator of iron homeostatis. Indonesia have more than 2.000 medical plants which have many roles and function. Some plants have phytochemisty that potentially in hepcidin antagonis. The phytochemistry is screen using molecular docking method Methods: The research design was a biocomputasion. It had been done at Medical Faculty of Sebelas Maret University on August-Oktober, 2014. The data was collected by using purposive sampling method. All data is docked using Autodock Vina in PyRx software. Docked sample is compared with binding energy of furstulhiamine drug. The data was compared its binding energy and their zone interaction with fursulthamine Results: The result were 10 samples has higher its binding energy than fursulthiamine. The binding energy of fursulthiamine was -6,7 kcal/mol. The highest binding energy was -8,5 kcal/mol and the lowest was -8 kcal/mol. All 10 sample interacted with cys 326. The cys 326 was the residue which can inhibit hepcidin interaction with ferroportin Conclusions: The Molecular Docking can be used to intial screening many phytochemisty. The result was 10 phytochemistry Indonesian medical plants which may inhibit hepcidin interaction. Further research is needed to prove the effect of phytochemistry. Keywords: Hepcidin, molecular docking, phytochemistry 
Molecular Docking Analysis of Liliaceae FamilyPhytochemicals to Estrogen Receptor α on Breast Cancer Compared to Genistein Permana, Bryan Pandu; Pesik, Riza Novierta; Hakim, Fikar Arsyad
Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Breast cancer is the second highest cancer incidence in the world with 1.7 million women were diagnosed in 2012. Interaction between estrogen and estrogen receptor α (ERα) have an important role in cancer progressivity and resistancy to chemotherapy. Genistein is one of the phytoestrogen that has selective estrogen receptor modulator (SERM) activity so it can inhibit the activity of estrogen receptor α (ERα) on the breast cancer. Other phytochemicals are expected to have more affinity to ERα than genistein. Anticancer activity of Liliaceae family phytochemicals have been found but its inhibition to ERα is unknown.  This research analyzes the interaction between Liliaceae phytochemicals to ERα by molecular docking technique. Method: This research is bioinformatics observational research that observed the interaction between Liliaceae phytochemicals to ERα. The molecular docking analysis was performed using PLANTS 1.1, preparation program MarvinSkecth 5.2 and YASARA 10.1. Visualization of the molecular docking was performed using PyMOL 1.3. Results: Six substances have lower docking score than genistein (-79.21). Those are spiraeoside (-84.80), stigmasterol (-84.28), cyanin (-83.79), beta-sitosterol (-82.81), progesterone (-82.68) and idaein (-82.22). Spiraeoside, cyanin, and idaein have one or more hydrogen bond to ERα in visualization. The six substances bind ERα on four same amino acids that bindgenistein, those are Trp383, Leu525, Met528, and Cys530. Conclusio: Molecular docking analysis of Liliaceae phytochemicals found six substances that have higher affinity to ERα than genistein with three substances have hydrogen bond and similar structure to the genistein. Keyword: Breast Cancer, Estrogen Receptor α, Genistein, Liliaceae Family Phytochemical, Molecular Docking.
In Vitro Inhibition Effect of Coconut Husk Ethanol Extract towards the Growth of Escherichia coli and Staphylococcus aureus from Surgical Site Infection Jatiningrum, Alifiana; ., Maryani; Ayusari, Amelya Augusthina
Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Surgical site infection was defined clinically, namely the existence of purulent discharge around the wound or wound cellulitis in operation. Coconut husk are known to has tannin and flavonoid as antibacterial properties. This study aimed to determine In Vitro effect of coconut husk (Cocos nucifera L.) ethanol extract towards the growth of Escherichia coli and Staphylococcus aureus from surgical site infection. Methods: This research is an experimental laboratory (post test only control group design) with a non-probability sampling technique namely consecutive sampling. Research conducted at the Microbiology Laboratory FK UNS and sampling conducted in the Microbiology Laboratory Dr. Moewardi Hospital. The independent variables are the coconut husk ethanol extract at a concentration of 1%, 20%, 40%, 60%, and 80%. Subjects were Escherichia coli and Staphylococcus aureus of surgical site infection standardized with Mc Farland 0.5. Test sensitivity on Muller-Hinton medium diffusion method with antibiotics cefoperazone/sulbactam and vancomycin as a positive control and DMSO 2% as negative control. Research results are tested with the Kruskal-Wallis test followed by Mann-Whitney test. Results: Coconut husk ethanol extract can’t inhibit the growth of Escherichia coli but can inhibit the growth of Staphylococcus aureus. Based on the results of the Kruskal-Wallis test that at least there is a significant difference in inhibition between the two treatment groups (p <0.005). The results of the Mann-Whitney test showed that there were significant differences in inhibition (p <0.05) in all treatment groups, except in the group with the inhibition of the extract concentration of 40% with 60% extract concentration did not reveal any significant differences in inhibition (p > 0.05). Conclusion: Coconut husk ethanol extract can’t inhibit the growth of Escherichia coli but can inhibit the growth of Staphylococcus aureus from surgical site infection by In Vitro. Keywords: Surgical site infection, Escherichia coli, Staphylococcus aureus, coconut husk
Relationship Between Dermatoglyphic Patterns and β-Thalassemia Major Disease Handayani, Selfi; Wiyono, Nanang
Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: β-Thalassemia major is the most common severe thalassemic disease in the world. Children with β-Thalassemia major have to be identified and treated soon to prevent the worse prognosis of the disease. One of the screening methods to establish thalassemic diagnosis is by observing dermatoglyphic patterns. The aim of this study was to determine the relationship between dermatoglyphic patterns and β-Thalassemia major disease. Methods: This study was an observational analytic study with cross sectional design. This study located in Poliklinik Anak and SMF Anak RSUD Dr. Moewardi Surakarta. The sampling technique used purposive sampling based on the characteristics of population that have been known previously. The subjects were 20 patients of β-Thalassemia major and 20 non- β-Thalassemia major. Data were obtained through direct scanning the subject’s fingerprints using scanner. Statistical analysis used independent-sample t test, Mann-Whitney test and chi square test. Results: From the analysis, this study found there was no significant difference of the ridge count between patients and control group (p > 0.05). However, there was significant difference of the patterns between patients and control group (p < 0.05). The number of whorl patterns in  β-Thalassemia major patients was 53,5%, it greater than that in normal individuals (20,5%). Loop patterns were less frequent in β-Thalassemia major patients (34%) compared to normal individuals (75,5%). Arch patterns had no significant difference between patients and control group (p > 0.05). Conclusions: There was a significant relationship between dermatoglyphic patterns and β-Thalassemia major disease. Keywords: β-thalassemia major, dermatoglyphic, fingerprint 

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