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AGRIVITA, Journal of Agricultural Science
Published by Universitas Brawijaya
ISSN : 01260537     EISSN : 24778516     DOI : https://doi.org/10.17503
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
AGRIVITA Journal of Agricultural Science is a peer-reviewed, scientific journal published by Faculty of Agriculture Universitas Brawijaya Indonesia in collaboration with Indonesian Agronomy Association (PERAGI). The aims of the journal are to publish and disseminate high quality, original research papers and article review in plant science i.e. agronomy, horticulture, plant breeding, soil sciences, plant protection and other pertinent field related to plant production. AGRIVITA is published three times per year. The Journal has been indexed in SCOPUS, Scimago Journal Ranks (SJR), Emerging Source Citation Index ( ESCI-Web of Science), EBSCO, ProQuest, Google Scholar and others international indexing. AGRIVITA is accredited first grade (Sinta 1/S1) for five years (2018-2023) based on Decree No: 30/E/KPT/2018 by Ministry of Research, Technology and Higher Education (Ristek Dikti), The Republic of Indonesia. We accept submission from all over the world. All submitted articles shall never been published elsewhere, original and not under consideration for other publication.
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Articles 804 Documents
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PEROXIDASE ISOZYME IDENTIFICATION OF SOME RICE GENOTYPES IN M1 GENERATION UNDER DROUGHT STRESS LEVEL OF -0.03 MPa Herwibawa, Bagus; Haryanto, Totok Agung Dwi; Sakhidin, Sakhidin
AGRIVITA, Journal of Agricultural Science Vol 36, No 3 (2014)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

The effort to fulfill the need of rice through the improvement of dryland productivity can be viewed as a more environmentally-friendly way. This research used 36 rice genotypes in M1 generation that were grown hydrophonically under drought stress level of -0.03 MPa. The identifications were conducted based on peroxidase isozyme marker. The isozyme patterns in zymogram were binary-coded by visual scores for each genotype, based on the thickness and the number in the appearance of bands on certain migration distance. The migration distances were measured based on values of Rf. The similarity coefficients were calculated using Dice’s coefficient that were used to construct dendrogram using the UPGMA employing the SAHN from the NTSYSpc 2.02. The results showed that the most resistant genotype under drought stress was R-4, and the genetic relationships among the genotypes were divided into two main groups, aromatic and non-aromatic group, in which some genotypes experienced the reduced levels of aromatic character (R-8 and R-9) and the drought resistance character (IU-2, IU-3, IU-4, IU-5, IU-6, IU-7, and IU-8), but there were some genotypes to able to improve the resistance under drought stress (R-2, R-3, R-5, R-6, R-7, IT-4, IT-5, and IT-7). Keywords:  drought resistance, gamma irradiation, peroxidase isozyme, rice, sodium azide
ABILITY TEST OF SEVERAL ANTAGONISTS TO CONTROL POTATO BACTERIAL WILT IN THE FIELD Soesanto, Loekas; Manan, Abdul; Wachjadi, Muljo; Mugiastuti, Endang
AGRIVITA, Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

The research objective was to know ability of antagonistic microbes to control bacterial wilt on potato in the field. This research was carried out at Serang Village, Karangreja Subdistrict, Purbalingga Regency from June up to August 2012. The antagonist, originally isolated from potato field, was Bacillus sp. B2 and B4, and Pseudomonas sp. P19 and P20. Based on the research result, Pseudomonas P19 could control the disease on potato with delaying incubation period of 78.95%, suppressing disease intensity of 51.57%, decreasing final pathogenic population of 99.74%, and inducing plant resistance with increasing saponin, tannin, and glycoside content. However, the antagonist could not increase growth and yield of potato.  Keywords: antagonistic microbes, bacterial wilt, potato
Instruction for Author Trisnadi, Rizki
AGRIVITA, Journal of Agricultural Science Vol 33, No 2 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

AN INTERACTION MODEL BETWEEN ENVIRONMENTAL FACTORS AND BLACK RICE GROWTH IN IRRIGATED ORGANIC PADDY FIELD Budiman, Budiman; Arisoesilaningsih, Endang
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Black rice production in organic farming system does not meet the demand of local customers because of its low productivity. This research aimed to set an interaction model using multivariate analysis via smartPLS to identify environmental factors which simultaneously affects the growth of black rice. The growth of black rice in two irrigated organic paddy field in Malang, Indonesia was observed during planting period from November 2011 to March 2012. In each rice field, the growth was periodically recorded during planting periods: 19-29 days after planting (dap), 41-45 dap, 62-66 dap, 77-81 dap, 90-94 dap and 104-106 dap. Environmental factors such as water quantities, soil conditions, weed communities and cultivation system around the black rice population were also measured. Black rice growth was influenced simultaneously by water quantities, soil, weed communities and cultivating systems with predictive-relevance value reaching 92.83%. Based on the model, water quantities in paddy field is a key factor which directly and indirectly determined the growth and productivity of black rice.Keywords:  black rice growth, environmental factors, interaction model, organic paddy field
THE POTENCY OF Bacillus sp. AND Pseudomonas sp. AS BIOLOGICALCONTROL AGENTS AGAINST CORN LEAF BLIGHT DISEASE CAUSED BY Pantoea sp. Javandira, Cokorda; Aini, Luqman Qurata; Sugiharto, Ariffin Noor; Abadi, Abdul Latief
AGRIVITA, Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

One of new biotic constraints in corn production in Indonesia is leaf blight disease caused by Pantoea sp. which is needed to be controlled. The purpose of this research is to study the potential of Bacillus sp. and Pseudomonas sp. as biological control agents against corn leaf blight caused by Pantoea sp. The results showed that all bacterial strains of Bacillus sp. and Pseudomonas sp. have potential in inhibiting the growth of Pantoea sp. by showing the clear zone on the agar plate. The antibiosis types are bactercide or bacteriostatic. On pot experiment all bacterial strains showed the reduction of the disease incidence at the same level compared with that of bactericide streptomycin suphate. All bacterial strains as well as bactericide could reduce the disease incidence at 18-24% compared with that of control (aquades treatment only). The results suggest that all bacterial strains are potential as biological control agent against leaf blight disease on corn leaf caused by Pantoea sp.Keywords: Biological control, Bacillus sp., Pseudomonas sp. and Pantoea sp.
Physic Nut Thrips Diversity Asbani, Nur; Sartiami, Dewi
AGRIVITA, Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Physic nut (Jatropha curcas L.) is a relatively new agricultural crop commodity in Indonesia. Hence, the thrips associated with this plant are not well recognized. The research objective was to provide information and tool for identification of thrips associated with physic nut. Survey method was conducted in some areas of Java, Madura, and Lombok island. The results showed that 10 species of thrips were found associated with physic nut. They belonged to nine genera and three family i.e. Thripidae, Aeolothripidae and Phlaeothripidae and one unidentified species. The Thripidae species were Selenothrips rubrocintus, Rhipiphorothrips pulchellus, Thrips hawaiiensis, Heliothrips haemorrhoidalis, Zaniothrips ricini and Scolothrips sexmaculatus. The family Aeolothripidae were Franklinothrips vespiformis, F. variegatus and Mymarothrips bicolor; the only one of Phlaeothripidae was Ecacanthothripstibialis. The identification key had been built for all species mentioned above. These thrips can be grouped into herbivore, carnivore, and fungivore.Keywords: Jatropha curcas, thrips, diversity
HYPOVIRULENT ISOLATES OF FUSARIUM COLLECTED FROM CHILI CROPS IN BOYOLALI REGENCY, CENTRAL JAVA, INDONESIA Supyani, Supyani; Widadi, Sri
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Fusarium, a genus of filamentous fungi, has many species which serving as important pathogens to many diseases in crops. Till today, there have not been effective and efficient control methods for such fungi. Recently, scientists agree that application of biological agents is a tactful choice. Development of hypovirulent strains of fungus as biocontrol agents is very limited. This research was aimed to find hypovirulent isolates of Fusarium from field as biological agents. A hundred isolates of Fusarium from chili were collected in Boyolali, Central Java. Morphological characterization revealed that isolates performed varied colony phenotypes. Based on colony phenotype pattern, isolates were classified into five groups. From each group, one hypovirulent isolate was selected based on colony growth rate on potato dextrose agar media. The selected hypovirulent isolates were used for virulence assay in apple. The result showed that there were four hypovirulent isolates i.e.: B6, C15, D19, and E20 isolates. Total RNA extraction of the identified hypovirulent isolates revealed the existence of viral RNA in C15 isolate. Based on the existence of viral RNA in C15 isolate, the hypovirulent traits were due to mycoviral infection, whereas the hypovirulent traits performed by the other three were due to genetic factors. Keywords: biological control agent, Fusarium, hypovirulent isolate, virulence assay 
PATHOTYPE GROUPING OF Xanthomonas oryzae pv. oryzae ISOLATES FROM SOUTH SULAWESI AND SOUTHEAST SULAWESI Khaeruni, Andi; Wijayanto, Teguh
AGRIVITA, Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important rice disease, and has caused significant economic losses.  This research aimed to determine the pathotype grouping and the distribution of Xoo isolates of South and Southeast  Sulawesi. In order to obtain the information, 61 Xoo isolates of South Sulawesi and 29 isolates of Southeast Sulawesi were evaluated for their pathotype grouping against 5 diffential varieties. Research results showed that in South Sulawesi there were 2 pathotype groups, namely pathotype IV (32.79%) and pathotype VIII(67.21%).  Pathotype VIII was widely distributed over the Western and Central areas of South Sulawesi, whereas pathotype IV was widely distributed over the Southern area.  In Southeast Sulawesi, it was found 5 pathotypes, namely pathotypes IV (27.58%), VI (10.34%), VIII (13.79%), IX (20.68%), and X (27.58%), with a limited and scattered distribution pattern on several areas. These results indicate that  Xoo pathotype groups in South Sulawesi and Southeast Sulawesi are varied and tend to sift to more virulent pathotypes. Keywords: bacterial leaf blight, pathotype group, Xanthomonas oryzae pv. oryzae
Study of Expression of Sugarcane Sucrose Transporter cDNA in Yeast Slameto, Slameto; Sugiharto, Bambang; Basuki, Nur; Sulistyowati, Liliek
AGRIVITA, Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Studies in transgenic sugarcane (Saccharum officinarum L.) demonstrated that sucrose transporter (SUT) genes were essential in sucrose translocations. Sucrose transporter gene isolated from sugarcane were designated as SoSUT1 and SoSOSUT2 respectively as encode protein of 518 and 747 amino acids. The genes were constructed into plasmid pYES2 for SoSUT1, and pYX112 for SoSOSUT2. cDNA SoSOSUT2 had also constructed into plasmid pBIN-At-GFP which was possible to trace the gene inserted. The constructed plasmid was transformed into yeast (Saccharomyces cerevisiae) and grown in minimal medium (SD–urasil) as selection medium. The transformed colony was confirmed using PCR. The functional expression was studied by growing yeast in YPD medium with 2% Sucrose, then the sucrose uptake was measured in number interval time using resorcinol method. The result showed that yeast INVSc1-pYES2-SoSUT1 and BF264- pYX112-SoSOSUT2 had higher ability in sucrose transport compared to the control-INVSc1 for SoSUT1 and control-BF264 for SoUT2. Moreover, the result showed that SoSUT1 had higher ability to transport sucrose than SoSOSUT2. Confocal microscope observation showed that transformation gene SoSOSUT2 was successful, which was indicated by green exposure of GFP protein.Keyword: sugarcane, sucrose transporter, yeast
PATH-ANALYSIS ON SEVERAL CHARACTERS IN POTENTIAL OF CORN PRODUCTION AND RESISTANCE TO DOWNY MILDEW Pudjiwati, Eko Hary; Kuswanto, Kuswanto; Basuki, Nur; Sugiharto, Ariffin Noor
AGRIVITA, Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

This research was aimed at investigating both direct and indirect impacts, and heritability values of characters regarding the potential of corn production and resistance to downy mildew. The result of this investigation is required to determine some criteria taken into account for selection process of downy mildew-resistant corn breeding with high yield. The field experiment was conducted at Research Centre of Agriculture Faculty, Brawijaya University from January to April 2012. Five varieties of hybrid crown and five inbreeding lines were employed, and Randomised Block Design was applied with two replications. As observed, the characters held heritability ranging from average to high, except for heritability of length and width of stomata on the lower surface of the leaves which was categorised as low. Moreover, the stomata density found on lower surface of the leaves was directly and positively correlated to the intensity of attack by downy mildew, which, then, was used as criteria selection in downy mildew-resistance. The intensity of disease and the density of the stomata on lower surface of the leaves accounted for direct and negative correlation to corn production, while the length and diameter of corncob was responsible for direct and positive correlation to corn production. The betterment of corn production can be coped by improving the plant resistance to downy mildew and characters of corncob diameter.Keywords: path-analysis, corn, downy mildew  

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