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INDONESIA
International Journal of Biosciences and Biotechnology
Published by Universitas Udayana
ISSN : 23033371     EISSN : 26559994     DOI : -
Core Subject : Science, Social,
International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences
Arjuna Subject : -
Articles 5 Documents
Search results for , issue "Vol 1 No 1 (2012)" : 5 Documents clear
DNA MICROARRAY BASED EXPRESSIONAL PROFILING OF HRPXO DEPENDENT UP-REGULONS IN XANTHOMONAS ORYZAE PV. ORYZAE Byoung Ho So; Jongun Kim; Hee Wan Kang
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2012)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Thirty-mer oligonucleotides for microarray analysis were designed from the annotated open reading frames(ORFs) in the whole genome sequence of X. oryzae pv. oryzae KACC10331. Mutant hrpXo::TN and wild-typestrain KACC10859 were cultured in hrp inducing medium (XOM2), and cDNAs, which were synthesizedfrom the total RNA samples from both strains, were hybridized on a DNA microarray. The microarraydata showed that 210 genes were down-regulated more than 2-fold in hrpXo::TN, while 115 genes wereup-regulated more than 2-fold. The HrpXo regulons diff erently included 54 hypothetical genes: type IIIsecretory genes (11 hrp genes); genes encoding type III secretory eff ectors (xopP genes and avr/pthA); genesencoding type II secretory eff ectors (6 proteases, a lipase, a polygalacturonase, and 4 cellulases); 7 iron-uptakegenes; 6 pil genes encoding fi mbrial assembly membrane proteins; and 14 transposon genes. Signifi cantplant-inducible promoter (PIP) sequences were newly identifi ed on HrpXo regulons. The validity of theexpressional profi les was further confi rmed by reverse transcription (RT)-PCR.
A PERSPECTIVE ON GLOBAL FOOD SECURITY-WHERE FROM HERE? Acram Taji; Tanya Tapingkae
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2012)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

A PERSPECTIVE ON GLOBAL FOOD SECURITY-WHERE FROM HERE?
UTILIZATION OF ELECTRIC PULSED POWER ON FRUITING OF EDIBLE MUSHROOMS Shoji Ohga
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2012)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Eff ect of pulsed power was investigated on fruit body formation of 10 edible mushrooms, Lentinula edodes,Glifola frondosa, Pholiota nameko, Flammulina velutipes, Hypsizygus marmoreus, Pleurotus ostreatus, Pleurotuseryngii, Pleurotus abalones, Agrocybe cylindracea and Sparassis crispa. Pulsed power of 100-170 kV was directlycharged to the substrate just before fructifi cation. The eff ect of the pulsed power resulted to promote for 10edible mushrooms fructifi cation. The treatment especially stimulated the fructifi cation on Pleurotus species.
EGG PRODUCTION, QUALITY AND CHICK COLOUR PLUMAGE OF FIVE COLOURS OF KAMPONG HENS FED GOOD QUALITY FEED IM Mastika; AW Puger; IKM Budiasa; IGL Oka
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2012)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

An experiment was carried out to study the egg production, quality and chick colour plumage of fi vephenotypic plumage colours of Kampong hens fed good quality feed. A Randomized Block Design consistedof fi ve treatments and fi ve replicateswere used in this experiment. A total of 25 maturated kampong chickensconsisted of fi ve plumage coloursi.e. Black (Selem), Red (biying), Mix colour (brumbun), white plumage withyellow leg (putih siung), and white plumage with white leg (putih kedas) and fi ve cockerels with respectivecolours were used in this experiment. Hens were housed individually in enrich cage. Mating procedurewas done in a special mating cage where each cockerel with respective colour stays all the time. Hen wasbring into the mating cage and left there for 24 hours and repeated after 5 days, until hens were going intobrood. A good quality feed contained 17% CP and 2750 kcal ME were off ered to all hens and fresh waterwere provided ad libitum. Observation was made for 14 weeks.This study showed that in one laying cycleexcept the mixed plumage colour hens produced similar number of eggs while the mix colour was the least.All parameters measured for egg quality from those fi ve plumage colours were similar. Observation ofthe chicks colour at one day old chick (DOC) showed that black plumage, white plumage with yellow leg,and red plumage hens had 100%, 100% and 81% chicks respectively had similar colour to their respectiveparents. While those mix colour and white plumage with white leg were only had 38.89% and 30.2% chickswith similar colour to their respective hens. After 8 weeks old, the colour of the chicks from respectivecolour has changed where black, red, white plumage with yellow leg had 70%, 77.5% and 93.75% chickswith the same colour to their respective hens. While those mix colour and white plumage with white leghad 53.8% and 17.6% chicks with the same colour to their parent. It was concluded that black, red, and whiteplumage with yellow legs had dominant gen in kampong chickens.
EXTRACT ETHANOL SEAWEEDS LOWERING LEVEL OF SERUM HMG-CoA REDUCTASE ENZYME OF WISTAR RATS HYPERCHOLESTEROLEMIA Ketut Sri Marhaeni Julyasih; I Gede Putu Wirawan
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2012)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Hypercholesterolemia or hyperlipidemia has been established as an important risk factor of cardiovasculardisease. Patients with hypercholesterolemia usually require a prolonged treatment; and the newer and morepotent generation of antilipid agents is costly. In Bali there are several types of seaweed that are generallyconsumed by local people and known by local names of Bulung Boni (Caulerpa spp.) and Bulung Sangu(Gracilaria spp.). Effect of Bulung Boni and Bulung Sangu extracts to reduce level of HMG-­??CoA reductaseenzyme are still very limited both in extent and depth, and further investigations were considered relevantand needed. This experimental study used completely randomized design, using a total of 24 Wistar ratsdivided into six sample groups of equal size, all fed with a diet high in cholesterol content. The six samplegroups were respectively designated as negative control group, positive control group, and four treatedsample groups, respectively, fed orally with a dose of 20 mg and 60 mg extracts of Bulung Boni per 100g ofbody weight per day, and 20 mg and 60 mg extracts of Bulung Sangu per 100g body weight per day. Eachtreatment was repeated four times. Results showed that rats fed with high-­??cholesterol diet and treated withoral Bulung Boni or Bulung Sangu extract at a dose of 20 mg and 60 mg/100 g bw/ day were statisticallysignificantly decrease serum HMG-­??CoA reductase enzyme levels (p < 0.05) as compared with those of ratsfed with high cholesterol diet without treatment with Bulung Boni or Bulung Sangu extracts. It implied thatBulung Boni and Bulung Sangu extracts decrease level of HMG-­??CoA reductase enzyme by significantlylowering serum HMG-­??CoA reductase enzyme levels.

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