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International Journal of Biosciences and Biotechnology
Published by Universitas Udayana
ISSN : 23033371     EISSN : 26559994     DOI : -
Core Subject : Science, Social,
International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences
Arjuna Subject : -
Articles 6 Documents
Search results for , issue "Vol 10 No 2 (2023): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY" : 6 Documents clear
PATHOGENITY TESTING OF THE MUSHROOMS Trichoderma spp. SOME PLANTATION LAND IN CONTROLLING Fusicoccum sp DISEASE OF TOBACCO PLANT Aisar Novita; Efrida Lubis; Le Huu Phuoc
International Journal of Biosciences and Biotechnology Vol 10 No 2 (2023): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v10.i02.p04

Abstract

Trichoderma is a soil saprophytic fungus that is naturally parasitic and attacks many types of plant disease-causing fungi or has a broad spectrum of control. This study aims to obtain superior isolates of the antagonist fungus Trichoderma sp from several plantations. This study used a non-factorial completely randomized design consisting of seven treatments with three replications. The treatments tested were: No Treatment (To); Trichoderma sp isolate from Langkat rubber (T1) Trichoderma sp isolate from Palm Oil Langkat (T2), Trichoderma sp isolate from Cocoa Langkat (T3), Trichoderma sp isolate from Percut oil palm (T4), Trichoderma sp isolate from Karo Coffee (T5), Trichoderma sp isolates from Karo cocoa (T6) Parameters observed were identification of the fungus Trichoderma sp., pathogenicity test on tobacco plants and percentage of pathogen inhibition. The results of both macroscopic and microscopic identification of Trichoderma sp isolates from rubber plantations in Langkat area (T1) showed that the highest percentage of inhibition and pathogenicity tests against Pussicoccum sp disease of tobacco plants showed negative results (no necrotic spots).
Identification of Phytochemical Compounds of Ethyl Acetate Extract of Bulung Anggur (Caulerpa Sp.) By Gc-Ms And Toxicity Test On Artemia Salina Leach Shrimp Larvae Ester Giwo; Nguyen Ti Ha; I Gede Putu Wirawan; I Nyoman Wijaya; I Ketut Suada
International Journal of Biosciences and Biotechnology Vol 10 No 2 (2023): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v10.i02.p05

Abstract

Sea grape (Caulerpa sp.) is one of the potential types of seaweed. Sea grape potential as a natural ingredient for making medicine. Balinese people use sea grape for consumption. The content of bioactive compounds in sea grape is suspected to be toxic at certain doses. The purpose of this study was to determine the compound content of ethyl acetate extract of sea grape (Caulerpa sp.) and the toxicity on Artemia salina Leach. The identification of the bioactive compounds was carried out by Gas Chromatography-Mass Spectrophotometry and the toxicity test of extracts was carried out using the Brine Shrimp Lethality Test (BSLT) method. The results showed that the ethyl acetate extract of sea grape (Caulerpa sp.) contained 27 chemical compounds, 7 of which had a quality value > 70%, namely Propanoic acid, ethyl ester; n-Propyl acetate; sec-Butyl acetate; Toluene; Acetic acid, butyl ester; Bicyclo [4.2.0] octa-1, 3, 5-triene; and Styrene. The results of the toxicity test of Artemia salina Leach are toxic which had an LC50 value of 44,070 ppm so that they can be used as a larvicide
Effects of Trichoderma Harzianum and Fusarium Solani Inoculant on the Resin Content of Agarwood (Gyrinops Versteegii (Gilg.) Domke) I Made Mega; Ni Luh Kartini; Ni Luh Putu Citra Innosensia
International Journal of Biosciences and Biotechnology Vol 10 No 2 (2023): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i02.p01

Abstract

This study aimed to determine which fungal inoculants, Trichoderma harzianum or Fusarium solani in a solid or liquid form, produced the highest resin content in agarwood trees. This experimental study used a randomized completely block design (RCBD) with seven treatments in triplicates: solid Trichoderma harzianum inoculants in pellets(A) and capsules (B), liquid T. harzianum inoculants (C), solid Fusarium solani inoculants in pellets (D) and capsules (E), liquid F. solani inoculants (F), and control or without inoculants (G). Parameters observed were the color and aroma of the infected agarwood and the resin content of the harvested sapwood. Quantitative data obtained from observations/measurements were analyzed statistically with analysis of variance and a subsequent LSD test at a 5% significance level following a significant result. The results showed that treatments using different fungal inoculants had no significant effects on the resin content of agarwood. However, inoculants in pellets and capsules tended to increase the resin content. The resin content of agarwood ranged from 15.97% to 21.53%, and the highest level was obtained from treatment A (solid T. harzianum inoculants in a pellet form)
Phytochemical Analysis of Bulung Boni (Caulerpa cylindracea S.) N- Hexane Extract with Gc-Ms Method and Toxicity Test on Mice (Mus musculus L.) May Disa Br Silalahi; I Gede Putu Wirawan; I Nyoman Wijaya; I Ketut Suada; Trisna Agung Phabiola; Angelika Astaykina
International Journal of Biosciences and Biotechnology Vol 10 No 2 (2023): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v10.i02.p06

Abstract

One of the marine resource commodities owned by Indonesia is seaweed. Bulung boni is one type of seaweed that grown in Indonesia. Bulung boni is the regional name of Caulerpa cylindracea. Bulung boni is usually used as a food and medicine for most people in Bali. The purpose of this research is to study the content of phytochemical compounds in n-hexane extract of bulung boni and determine the toxicity effect on mice (Mus musculus). The methods used in this study were maceration, GC-MS (Gas Chromatography-Mass Spectrometry), observation, and calculation with the Thomson and Weil formula. This study used 24 mice as experimental animals. Mice were divided into 6 treatment groups, group 1 as control was given 1% Na-CMC solution, groups 2-6 were given test extracts with doses, 100, 200, 300, 400, 500 mg/30 g BW mice. The results showed the highest compound contained in the n-hexane extract of bulung boni was Hexadecanoic acid, methyl ester with an AUC value of 35.21%. Toxicity tests on mice showed no deaths experienced by mice, so the LD50 value determined was pseudo at 16.6 g/ kg BW and fell into the practically non-toxic category
Analysis Of The Addition Of Coca-Cola Sludge For Quality Compost Production I Dewa Made Arthagama; I Made Dana
International Journal of Biosciences and Biotechnology Vol 10 No 2 (2023): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v10.i02.p02

Abstract

Bali as a tourist destination makes a perfect location for the soft drink industry, Coca-Cola, to build their factory which is located in Mengwi District, Badung Regency, Bali, Indonesia. In the production process of the beverage industry, of course, it produces by-products in the form of solid waste sludge that is not utilized. This study aims to determine the compost formula with the addition of Coca-cola sludge to improve the quality of the compost. This research was conducted from June to July 2021, using a completely randomized design (CRD) consisting of 11 formulations of compost material, which was repeated 3 times so that 33 experiments were obtained. The results showed that the treatment G = (2.5 kg goat manure + 1.5 kg rice straw + 0.5 kg Coca-cola sludge) gave the best compost quality as indicated by the highest N, P, and K parameter values, the best C/N ratio, and pH approach to neutral. It should be tried to be applied in the field for plant growth and production using several plants and in several types of soil
Morphological and Chemical Characteristics of Porang Tubers (Amorphophallus oncophyllus) From Different Harvest Periods Gusti Setiavani; Budi Suarti; Mona Nur Moulia; Aisar Novita; Seca Gandaseca
International Journal of Biosciences and Biotechnology Vol 10 No 2 (2023): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v10.i02.p03

Abstract

Porang tubers (Amorphophallus oncophyllus) have recently garnered more attention with the increasing demand for their derivative products. This research aimed to determine the morphological and chemical characteristics of porang tubers from different harvest periods. The method used was a completely randomized design. Also, for comparison, the morphological and chemical characteristics of bulbils were obtained from previous studies. Based on the analysis, morphologically, the stem tubers have an oval shape developing at the base of the stem with yellowish-brown to orangish-brown skin. Bulbils have an irregular oval shape with brown skin and white spots. They all have similarly dark yellow fibers. The stem tubers in the first harvest period were smaller in diameter and lighter (479.20 ± 183.54 g) than in the second period (609.71 ± 169.42 g). In contrast, bulbils at the leaf axils are smaller (diameter = 3–3.7 cm, thickness = 1.9–2.4 cm) and only weigh about 12.3–25.3 g. Chemical analysis revealed that the flour made from the stem tubers contained 14.28–17.57% glucomannan and 9.16­–11.10% protein, generally higher than bulbils with 25.78% glucomannan and 9.52% protein (very low). The yields of porang flour were 0.15±0.02% and 0.14±0.01% from the first and second harvest.

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