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INDONESIA
International Journal of Biosciences and Biotechnology
Published by Universitas Udayana
ISSN : 23033371     EISSN : 26559994     DOI : -
Core Subject : Science, Social,
International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences
Arjuna Subject : -
Articles 9 Documents
Search results for , issue "Vol 4 No 1 (2016)" : 9 Documents clear
MORPHOLOGICAL CHARACTERS AND GENETIC VARIABILITY OF CHAMPACA IN BALI I Made Sukewijaya; Made Sudiana Mahendra; I Nyoman Rai; I Gede Rai Maya Temaja
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Bali community utilize champaca flower for offering materials and worship, besides beauty salon purposes, the SPA aromatic ingredients, essential oils, perfumes, cosmetics, and drugs. Various champaca plants in Indonesia have not been studied as one of Indonesia's biodiversity that can be used as excellent genetic resources (germplasm). The objective of the study was to determine the genetic diversity of champaca in Bali. The results revealed that 12 (twelve) champaca accession morphologically was characterized. All of accessions obtained from cultivation centers champaca in Bali. Based on the characteristic was observed by morphological characters i.e.: (a) Cempaka Putih Wilis (b) Cempaka Putih Pateh, (c) Cempaka Putih Patemon, (d) Cempaka Putih Sibang, (e) Cempaka Kuning Muda Petemon, (f) Cempaka Kuning Kecil Patemon, (g) Cempaka Kuning Besar Patemon, (h) (i) Cempaka Kuning Kecil Sibang, (j) Cempaka Kuning Tua Sibang, (k) Cempaka Kuning Muda Sibang, and (l) Cempaka Kuning Punah Sibang. Morphologically, champaca in Bali can be grouped into 4 clusters and therefore, and based on RAPD analysis champaca in Bali could be grouped into 5 clusters.
PHOSPHATASE ACTIVITY AND PHOSPHATE SOLUBILITY BY PHOSPHATE SOLUBILIZING RHIZOBACTERIA IN VOLCANIC SOILS OF PANCASARI, BALI Ketut Dharma Susila; I Made Sudana; Ni Putu Ristiati; I Made Adnyana
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Phosphatase in the soil was found as extracellular enzymes produced by soil micro-organisms both in acidic or alkaline conditions. Phosphatase is an enzyme complex that plays an important role in deciding soil-bound phosphate bond organic compounds to form orthophosphate which is available to the plant. Mineralization of organic forms into a inorganic-P determined by the ability of these bacteria to produce phosphatase. Therefore, observations of phosphatase activities are important to know how intensely P mineralization process takes place in the soil. Phosphate solubilizing rhizobacteria (PSR) has been widely known to affect mobilization of insoluble inorganic phosphates become available to plants. Although phosphate solubilizing rhizobacteria is widely available in the soil, their activity is usually not strong enough to compete with other bacteria commonly located in the root zone (rhizosphere). That is why, screening of phosphatase enzyme activity and various phosphate solubilizing ability to obtain better strains of bacteria are still needed. This study was conducted to determine the ability of phosphate solubilizing rhizobacteria, its potential as a biofertilizer inoculant and characterize its capacity to dissolve P-insoluble inorganic and produce phosphatase enzymes in the growing medium. Potential phosphate solubilizing rhizobacteria was tested for their ability in vitro using Pikovskaya media containing insoluble forms of inorganic phosphate as a source P. The results of this study indicate that there are some isolates phosphate solubilizing rhizobacteria that are capable of dissolving the insoluble inorganic-P with different abilities. The highest potential successively displayed by isolates TbPP-4.1; BdPP-2.1; and SBPP-1.3 that significantly has the same ability to soluble inorganic phosphate in the soil. The isolates BdPP-2.1 and TbPP-4.1 are significantly higher than the other isolates for producing phosphatase. Both isolates TbPP-4.1 and BdPP-2.1 have the same pontenty as a biofertilizer based on its capacity to mineralize forms of soil organic phosphorus by phosphatase activity.
Content Articles Content Articles
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Content Articles
GENETIC DIVERSITY OF GUAVA (Psidium Guajava L.) IN BALI INDONESIA BASED ON RAPD MARKER Ni Nyoman Ari Mayadewi; I Nyoman Rai; Rindang Dwiyani; Ida Ayu Astarini
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

The aim of this study was to investigate genetic diversity and relationship of guava (local name: jambu biji) genotypes grown in Bali, Indonesia, based on Random Amplified Polymorphic DNA (RAPD) markers. Twelve guava cultivars namely Jambu biji ‘Australia’, ‘Bangkok Merah’, ‘Bangkok Putih’, ‘Cokorde’, ‘Dadu 1’, ‘Dadu 2’, ‘Getas Merah’, ‘Kamboja’, ‘Kristal’, ‘Pipit’, ‘Susu’ and ‘Variegata’ were collected from nine Regencies in Bali using survey and explorative method. Ten decamers RAPD primers were employed to distinguish between the 12 cultivars and determine their genetic relationships. A dendogram was constructed using coefficient dissimilarity analysis based on phylogenetic analysis using parsimony (PAUP). The twelve cultivars were grouped into 2 main clusters and five smaller clusters. Variation between genotypes of guava local will be good sources for future crop improvemen.
CARBON STOCK DUE TO THE INTENSITY OF THE USE OF FOREST AREAS IN FOREST MANAGEMENT UNIT OF WEST BALI Wiyanti Wiyanti; Indayati Lanya; I Nyoman Merit; Made Antara
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

This study aimed to determine the magnitude of the changes in carbon stocks due to changes in forest utilisation. Location of the study include planted forests, coffee plantations, mix garden, and cajuput region. The method used in this study was to estimate carbon stock based on the weight of biomass both above the surface and underground. Measurements were made on the biomass of trees and undergrowth, necromass (dead plant parts), both woody and non-woody (litter), and reserve C in the soil. The results showed that there were considerable differences of carbon stock in each area utilisation. The highest carbon stock found in the mix garden (275.62 tonnes/ha), then decrease at mahogany forest (269.63 tonnes/ha), planted forests (231.45 tonnes/ha), old cajuput (Melaleuca cajuput) (118.53 tonnes/ha), trimmed cajuput (86.57 tonnes/ha), coffee plantations (74.37 tonnes/ha), and un-trimmed cajuput (56.78 tonnes/ha). The recommendation can draw out in this research are: ( 1 ) In the area of coffee planting, horticultural forestry can be developed in the form of rows of plants among the coffee plants and ( 2 ) cajuput planting can be done with the system surjan and each row of cajuput consists of 2 rows with a distance planting more tightly.
IN-VITRO INDUCTION OF GRAPEVINE (Vitis Vinifera L.) SHOOTS USING 2-iP Rindang Dwiyani
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

The research concerning induction of grapevine shoots grown in vitro have been conducted during period of May to July 2016 at The Laboratory of Plant Tissue Culture, Faculty of Agriculture, Udayana University. The objective of the research was to investigate the most appropriate explant position and the optimal concentration of 2-iP in stimulating of grapevine shoot in vitro. Nodal segment from grapevine plant grown in the green house was used as planting material. The experiment was laid out in the factorial design, with two factors. The first factor was the position of explant (lay-down and up-right position) and the second factor was concentration of 2-iP ( i.e. 0, 3, 6, 9, 12 ppm). Each combination was replicated ten times. The results showed that the treatment of 6 ppm 2-iP with up-right explant position was the most appropriate condition in stimulating growth of grapevine shoot. With up-right position of explant, the percentage of shoot producing-explants at 10 weeks after planting was 100% at the treatment of 6 ppm 2-iP, compared to 4%, 12%, 40%,and 40% at the treatment of 0 , 3, 9 and 12 ppm 2-iP respectively. The average number of shoot per explant was 5.5 for 6 ppm-treatment, while it was less than 3 for other treatments.
Application of the Revegetation Sheet with the Erosion Control Function to the Expansive Clay Soil Slope -A Case Study on Test Construction and Monitoring in West Java, Indonesia- Nobuyuki Kohno; Takashi Shimizutani
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

A new type of revegetation sheet which also has a function of soil erosion control was applied to the expansive clay soil slope in West Java, Indonesia, and the function of the sheet to reduce the collapse of the slope was examined. Firstly, it was identified that the soil in the test construction site was a typical expansive clay soil mainly consisting of smectite and quartz according to the X-ray diffraction analysis. Secondly, as a result of monitoring up to 13 months after the application of the sheet, it was observed that the sheet successfully controlled soil erosion and led to gradual vegetation recovery, despite the fact that the construction area is in a part of tropical monsoon climate where the rainy and the dry seasons come repeatedly in a year and the soil swells in rainy season and shrinks and forms crack in dry season. Therefore, it was indicated that as the recovery of vegetation went by, the effect of controlling soil erosion, land slide and slope collapse would be increased.
MAPPING LAND SUITABILITY OF SUBAK ON BASED ON GEOGRAPHYC INFORMATION SYSTEM (GIS) IN DENPASAR, BALI Ni Made Trigunasih; Indayati Lanya; I Nengah Netera Subadiyasa; I Made Adnyana
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

This study aims to identify and provide land suitability database of Subak to support sustainable agricultural development and provide spatial information such as land suitability maps with existing inhibiting factor. The method used was field survey to obtain data characteristics/quality of land through soil sampling on several land units sample, and soil analysis in the laboratory. Land suitability classification was done using the criteria from the Technical Instructions Evaluation of Land for Agricultural Commodities by matching between the quality/characteristics of the land with the growing requirements of rice crop being evaluated. The analysis of actual land suitability both of S2 (little suitable) with limiting factors included drainage, texture, depth of planting, salinity, N- available, P-available and K-available, and slope; and S3 (marginally suitable) with limiting factors such as N-available, P-available and salinity. The assumption of business improvements that can be made to the determine quality/characteristics of the land Subak was an inhibiting factor, with optimization by improved drainage channels, N and P in accordance with the needs of the rice plant. Therefore the suitability of potential land for irrigated rice crops can be mapped by GIS and can be increased to very suitable (S1) level. In conclusions, the actual land suitability was categories as 72% little suitable (S2) and 28% marginally suitable or S3 with inhibiting factors included rooting medium, availability of nutrients, slope and salinity. While the suitability of potential land consisted of all of Subak that invery suitable (S1)level, so that the existence of Subak in Denpasar should be protected.
Guide for Authors Guide for Authors
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Guide for Authors

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