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INDONESIA
International Journal of Biosciences and Biotechnology
Published by Universitas Udayana
ISSN : 23033371     EISSN : 26559994     DOI : -
Core Subject : Science, Social,
International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences
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Articles 5 Documents
Search results for , issue "Vol 8 No 1 (2020): Vol. 8 No. 1, September 2020" : 5 Documents clear
PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF PURNAJIWA FRUIT EXTRACT (Euchresta horsfieldii (Lesch.) Benn.) Romulia Trielva Apriliani; I Gede Putu Wirawan; Wayan Adiartayasa
International Journal of Biosciences and Biotechnology Vol 8 No 1 (2020): Vol. 8 No. 1, September 2020
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2020.v08.i01.p04

Abstract

Antioxidant is a chemical compound that could give one or more electrons to inhibit the free radical reaction. One of the plants that potentially produce antioxidants is purnajiwa (Euchresta horsfieldii (Lesch.) Benn.). The research was conducted using the fruit of purnajiwa. The purpose of this study is to know antioxidant activity from purnajiwa fruit using the DPPH method and measured by UV-Vis spectrophotometry. The extract of purnajiwa fruit gained by the maceration method using 96% ethanol solvent. The results of phytochemistry screening found out that the extract contains flavonoid, alkaloid, saponin, tannin, and terpenoid. According to flavonoid testing using quercetin, the results obtained from purnajiwa extract contained flavonoid of 1,651 mg QE/g. The activity test of antioxidants quantitatively by DPPH using UV-Vis spectrophotometry found out that purnajiwa extract has IC50 value of 57,28 ppm so that purnajiwa extract has strong antioxidant activity that could be used as natural antioxidant resources.
IDENTIFICATION OF AGARWOOD SAPWOOD CHEMICAL COMPONENTS FROM FUNGAL INOCULATION RESULTS ON Gyrinops versteegii (Gilg.) DOMKE PLANTS I Made Mega; Ni Luh Kartini
International Journal of Biosciences and Biotechnology Vol 8 No 1 (2020): Vol. 8 No. 1, September 2020
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2020.v08.i01.p05

Abstract

Agarwood is one of non-timber forest product comodites (NTFPs) which has high economic value and as an export commodity. Agarwood produce an aromatic resin substance in the form of lumps that located between the wood cells in various shapes and colors. According to Indonesian National Standards, the category of agarwood is classified into several levels such as agarwood sapwood, kemedangan, and agarwood powder. The classification system is based on color, weight, and aroma. This classification method shows that the current quality of agarwood welding systems is still subjective. Therefore, more objective welding system is needed which is related to the chemical composition and resin content. This study objective is to identify the chemical components of agarwood sapwood from the results of fungal inoculation in agarwood-producing plants of gyrinops versteegii. The research conducted by extracting agarwood sapwood sample using acetone. This agarwood sapwood sample collected from the result of Fusarium, Rhizopus and Trichoderma fungi inoculation for 1 year. Furthermore, the agarwood acetone extract fractionated with a gradient column chromatography with hexane-ethyl acetate eluent. The method to identify the compounds/component is using chromatography gas-spectro-photometer mass (GC-MS). The results showed that the chemical components contained in agarwood from fungal inoculation were mostly in the form of ester compounds. The highest types compounds and the highest density compound were found in agarwood produced by Trichoderma fungi inoculant, namely Hexanedioic acid, dioctyl ester; 11-octadecanoic acid, methyl ester; Cyclopropanedodecanoic acid, 2-octyl-, methyl ester; 9-octadecanoic acid, methyl ester; Octadecanoic acid, 9.10- dihydroxy-, methyl ester; Cyclopropanepentanoic acid, 2-undecyl-, methyl ester; Pentadecanoic acid, 14-methyl-, methyl ester; and non-ester compounds, namely: 2-tridecenI-ol; 2- (2 ’, 4’, 4 ’, 6’, 6 ’, 8’, 8’Heptamethyltetrasiloxan -2 ’; 3-Hydroxybutanamide. 8- methoxy-2- (2.
TOXICITY TEST OF Gracilaria sp. AGAINST Aspergillus niger van Tieghem AND THE PHYTOCHEMICAL ANALYSIS Nevy Widya Pangestika; I Gede Putu Wirawan; I Ketut Suada
International Journal of Biosciences and Biotechnology Vol 8 No 1 (2020): Vol. 8 No. 1, September 2020
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2020.v08.i01.p01

Abstract

The purpose of this study was to know the effectiveness of Gracilaria sp. ethanol extract to inhibit the growth of Aspergillus niger. The results showed that the ethanol extract of Gracilaria sp. was not effective to inhibit the growth of A. niger. The minimum inhibitory concentration test (MIC) was carried out using extracts with 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7 %, 0.8 %, 0.9%, 1%, 2% and 0% percentage. The MIC results showed that the minimum extract inhibiting A. niger was 0.8%. The results of the antifungal activity test showed that the ethanol extract of Gracilaria sp. was fungistatic against A. niger. On the third day incubation, the 4% extract inhibited the growth of fungi with an average diameter of 5 mm. The fungal colony test was carried out using extract with 0.5%, 1%, 2%, 4% and 0% concentration, and the results showed that extract with 4% concentration can inhibit fungi colony growth by 69%. Phytochemical analysis was conducted using the UV-Vis Spectrophotometry, and the results showed that the ethanol extract of Gracilaria sp. contained 366.33 mg/100g/GAE phenol, 2041.47 mg/100g flavonoids, and 3041.60 mg/100g/TAE tannins. Tannins are suspected to be the most dominant fungistatic compound with the largest amount.
RUNNER-TIP CULTURE OF STRAWBERRY (Fragaria x ananassa Duch) GROWN ON SEVERAL SHOOT-INDUCTION MEDIUM Rindang Dwiyani; Hestin Yuswanti; Ni Nyoman Ari Mayadewi; Yuyun Fitriani
International Journal of Biosciences and Biotechnology Vol 8 No 1 (2020): Vol. 8 No. 1, September 2020
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2020.v08.i01.p02

Abstract

A research regarding “Runner-tip culture of strawberry (Fragaria x ananassa Duch) Grown on Several Shoot-induction Medium” has been investigated. The objective of the research was to find out the most suitable medium for shoot production from runner-tip culture of strawberry at establishment step of micropropagation. The research was laid out in a Completely Randomized Design, 4 treatments of medium type for shoot induction and 10 replication, each was represented by one (1) bottle with 6-8 explants. The treatments were summarized as follows: T1 = MS ; T2 = MS + 2 ppm BAP + 0.01 ppm NAA; T3 = MS + 1 ppm of TDZ; T4 = WPM + 2 ppm BAP + 0.01 ppm NAA. The parameters observed were days of the bud emergence, the average number of shoots per explant, and the average number of leaves per explant. It can be concluded that among medium used in the current research, the medium of MS added with 1 ppm thidiazuron (TDZ) was the most suitable medium for shoot production of strawberry from the explant of runner-tips. The treatment was resulted in the earliest time of bud emergence, and producing the highest number of shoots and leaves.
Citrus TISSUE CULTURE WITH TWO DIFFERENT APPROACHES Firoozeh Chamandoosti
International Journal of Biosciences and Biotechnology Vol 8 No 1 (2020): Vol. 8 No. 1, September 2020
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2020.v08.i01.p03

Abstract

Citrus is an important genius for economy and human health but a susceptible genius against biotic and abiotic stresses, so it needs to improved programs. Different basal media (MS, ½MA, ?MS and DKW) and different kind and concentrations of plant growth regulators i.e. BA, KIN, 2ip, ZE and TDZ (0 – 2 mg/l) and NAA, IAA and IBA (0 – 2 mg/l) added with 30 g/l sucrose, 3 g/l active charcoal and 7.5 g/l bacteriological agar] were used for organogenesis include shooting and rooting, also callusing from nodal explant of Citrus latifolia. MS medium supplemented with 1 mg/l BA, and 0.01 mg/l NAA is the best media for multiple shoot induction on nodal explants and elongation of them. Other cytokinins had not significant effects on shoot induction and multiplication. Using of 0.01 mg/l IBA instead of 0.01 mg/l NAA on medium with 1 mg/l BA, led to multiple shoot induction on nodal explant indirectly. Rooting was induced on DKW medium plus 1.5 mg/l NAA in the best way compared another media. Both direct and indirect organogenesis (multiple shoot induction) were carried out on media with very similar contents. So we can use very simple and practical methods tissue culture for different improved programs in Citrus genius genius.

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