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INDONESIA
JURNAL BIOLOGI INDONESIA
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Arjuna Subject : -
Articles 15 Documents
Search results for , issue " Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA" : 15 Documents clear
Pengaruh Suhu, pH, Enzim dan Surfaktan terhadap Plantarisin F Rekombinan Enkapsulasi sebagai Antibakteri Staphylococcus aureus dan Salmonella typhi Mustopa, Apon Zaenal; Hasim, Hasim; Amelia, Suci
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3664

Abstract

ABSTRACTStaphylococcus aureus causes diarrhea, which is one of the main cause of infant mortality, where asSalmonella typhi causes typhoid fever with the incidence rate of 180/100,000/year. Plantarisin F is anantimicrobial peptide that can inhibit the growth of S. aureus and S. typhi. The aim of this study is to determinethe effect of temperature, pH, enzymes, and surfactants of encapsulated F recombinant plantarisin. Plantarisin F(1.61%) encapsulated with maltodextrin (5.36%) and skim milk (2.68%) using the spray dry with inlettemperature 150ºC produced particles that are generally spherical with a rough texture, range in size, yield25.03%, and had good antibacterial activity against S. aureus and S. typhi. The antibakterial activity plantarisinF encapsulated is not affected by the treatment temperature (40ºC-100ºC), pH (2-12), enzyme (proteinase-K,catalase, lysozyme, pepsin, trypsin), dan surfaktan (SDS, urea, triton X-100, PMSF, EDTA) treatment.Keywords: Antibacterial, Encapsulation, Plantarisin F, Salmonella typhi, Staphylococcus aureus
Identifikasi Protein dan Seleksi Isolat Trypanosoma evansi Bersifat Imunogenik untuk Kandidat Pengembangan Imunoasai Subekti, Didik T.; Yuniarto, Ichwan
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1180.87 KB) | DOI: 10.14203/jbi.v14i1.3669

Abstract

ABSTRACTSurra is a parasitic disease caused by T. evansi and causing high economic losses in Indonesia. Some isolates have been isolated from several areas experiencing outbreaks of Surra in Indonesia. The isolates have been reported to have a diversity of protein profiles based on SDS PAGE (sodium dodecyl sulphate polyacrilamide gel electrophoresis). The research aims to identify immunogenic protein from each isolate and make the selection of T. evansi isolates which is potential as a source of antigens for immunoassay development. Each isolates were obtained to be purified from blood and then the protein was isolated. The proteins were run onto polyacrylamide gel electrophoresis and visualized by Coomassie blue. Another electrophoresis results were transferred onto a nitrocellulose membrane for immunoblotting. The results showed that the immunogenic proteins that consistently detected among nine T. evansi isolates of Indonesia are 100, 90, 85, 76-80, 70, 65, 55, 49-52, 44-46, 40, 34-36, 31 -33 kDa. Among the nine T. evansi isolates, N372 isolate was selected as a candidate for immunoasay development, especially ELISA. Immunogenic proteins were specifically found on the N372 isolate are 85, 70, 65, 49-52, 44-46, 34-36, 31-33, 24-28, 15-20 kDa.Keywords : Trypanosoma evansi, immunoblotting, protein profiles, immunogenic protein
Respon Tanaman Jewawut [Setaria italica (L.) P. Beauv.] terhadap Kondisi Cahaya Rendah Ridwan, Ridwan; Handayani, Tri; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1020.759 KB) | DOI: 10.14203/jbi.v14i1.3656

Abstract

ABSTRACTMillet [Setaria italica (L.) P. Beauv.] is one of the potential cereal crops to be developed in supporting food diversification program. The purpose of this study was to investigate the response of millet to low light (shade) condition. The experiment was conducted in the field with nested design that consisted of two factors that are, 1) Accession from Buru Island which are yellow seed and red seed; and 2) Shading nets that consisted of 0%, 40%, and 70% shade. Shade factor was designed as main plot, while accession factor was designed as sub plot. Growth (growing percentage, plant height, leaf number, leaf area, and plant dry weight), yield (panicle length and panicle weight), photosynthetic pigments (chlorophyll a, chlorophyll b, and carotenoid), and stomata density of the millet were observed. The result showed that the growth and yield of both accession were decreased by the increase of shading. However, yellow seed millet tended to be more tolerant than red seed millet with optimizing photosynthetic capacity through enhancing leaf area, leaf longevity, and enhancing antenna pigments such as chlorophyll b and carotenoid.Keywords: Growth, millet, photosynthetic pigments, shading, yield
Benthic Molluscs Communities in the Intertidal Coast of Tanimbar Islands, West Southeast Mollucas Heryanto, Heryanto
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.646 KB) | DOI: 10.14203/jbi.v14i1.3665

Abstract

ABSTRACTThis paper presents the results of a survey on intertidal molluscs in 3 habitats (Seagrass bed, coral reef, and sandflat) in Tanimbar Islands of West South-east Moluccas, Indonesia. Six relatively large and two small islands were attained to perform 125 plots of 34 stations. Molluscs’ diversity in each habitat were calculated using Shannon-Wiener formulation whereas Mann-Whitney formulation for comparing between habitats. Multivariate analyses for clustering of PAST 2.17 was utilized to further the analyses. A number of 101 species gastropods (26 families) and 51 species of bivalves (19 families) were recorded. Seagrass bed was inhabited by 100 species, followed by coral reef by 85 and sandflat by 44 species. Shannon-Wiener indexes for molluscs’ diversity range in seagrass was 0.39 to 1.29, in coral was 0.27 to 1.14 and in sandflat was 0.52 to 0.99. Clustering analyses for stations revealed that three stations in seagrass were the most dissimilar, followed by one station in coral due to habitat. Clustering analyses for species found four dissimilar groups of molluscs, whereas a group has many consimilar members.Keywords: Seagrass, coral, sandflat, diversity, clustering
Efek Ekstrak Metanol Makroalga Cokelat (Sargassum sp.), Merah (Gracillaria sp.) dan Taurin Terhadap Gambaran Histopatologi Hepar Mencit Jantan (Mus musculus) yang Diinduksi Benzo(?)Piren Hervidea, Radella; Widiastuti, Endang Linirin; Nurcahyani, Endang; Sutyarso, Sutyarso; Susanto, G. Nugroho
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3670

Abstract

ABSTRACTCancer is a disease marked by the existence of abnormal growth of cell continuously and uncontroll in an organism body. Benzo(?)pyrene is one of the substances that can cause a cancer. The methanolic extract of Sargassum sp. and Gracillaria sp. has anticancer and antioxidant activity. Other compounds with antioxidant activity is taurine. The aim of this research is to examine the effect from methanolic extract of Sargassum sp. and Gracillaria sp .and also taurine on hepar histopatology of male mice (Mus musculus) induced by benzo(a)pyrene. This research used Completely Randomized Design. 30 male mice were divided into 6 groups of treatments, those were: K1 = (K-), K2= induced by benzo(?)pyrene for 10 days, K3= after induced by benzo(?)pyrene, were given the Sargassum sp. orally with a dose 8mg/mice during 15 days, K4=after induced by benzo(a)pyrene, were given the combination of Sargassum sp. (8mg/mice) and taurine(15,6mg/mice) orally during 15 days, K5= after induced by benzo (?)pyrene, were given the Gracillaria sp.orally with a dose 8mg/mice during 15 days, and K6=after induced by benzo(a)pyrene, were given the combination of Gracillaria sp. (8mg/mice) and taurine (15,6mg/mice) orally during 15 days. Data were analyzed using ANOVA (? = 5%). The result shows that the combination of Sargassum sp. and Gracillaria sp. and taurine can increase the number of erythrocyte , decrease the number of leucocyte and protect the damage of hepar histopatology of male mice (Mus musculus) induced by benzo (?)pyrene. Combination of extract Gracillaria sp. and taurine is the most effective to protect the damage hepar histopatology of male mice(Mus musculus) induced by benzo(? )pyrene.Keywords: male mice,Sargassum sp., Gracillaria sp., taurine, benzo(a)pyrene, hepar
Efektivitas Proses Fitoremediasi Air Irigasi Tercemar Bahan Organik melalui Sistem Batch Culture menggunakan Hidromakrofita Lokal Retnaningdyah, Catur; Arisoesilaningsih, Endang
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.803 KB) | DOI: 10.14203/jbi.v14i1.3659

Abstract

ABSTRACTIrrigation water in Indonesia generally has polluted by organic matter due to human activities. The aim of study was to develop a package of phytoremediation technology that can effectively reduce organic pollutants in the irrigation water in order to ensure good quality of water irrigation to support organic farming. The study was conducted in greenhouses by planting some local emergent, submerged, floating-leaf hydromacrophytes and polyculture species using a batch culture system on a 30 L bucket with sand and gravel as a substrate. The study used a completely randomized design with the treatment were five types of hydromacrophytes (emergent such as Scirpus sp., Acorus calamus, Marsilea crenata; floating leaf macrophytes included Ipomoea aquatica, Azolla sp.; submerged such as Valisneria sp., Hydrilla verticilata; polyculture of the three hydromacrophytes, and controls without plant) which repeated three times at the same time. The effectiveness of the phytoremediation model for each treatment is determined by its ability to improve water quality as reflected by several physicochemical parameters of pH, DO, conductivity, turbidity, temperature, nitrate, orthophosphate, bicarbonate, BOD, TSS, TDS and TOM six days after incubation. Research results showed that batch culture for six days significantly reduced the organic matter content of irrigation water reflected from decreasing value of BOD, TOM, TSS, turbidity, nitrate, orthophosphate and bicarbonate levels. The six-day phytoremediation process in all treatments has not been able to increase the dissolved oxygen content in the water and has not been able to significantly reduce the conductivity and TDS. Hydromacrophytes submerged and polyculture were more effectively perform phytoremediation process compared with others.Keywords: Phytoremediation, local hydromacrophytes of emergent, submerged, and floating leaves, water pollution
Fermentasi Jali Menggunakan Bakteri Selulolitik dan Bakteri Asam Laktat untuk Pembuatan Tepung Handayani, Rini
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (671.656 KB) | DOI: 10.14203/jbi.v14i1.3666

Abstract

ABSTRACTJali (Coix lacryma-jobi L.) is a cereal plant. Jali has a high nutritional content. The protein content of jali is greater than sorghum, which is 11 g / 100 g and a high calcium content of 213 mg / 100 g. Cereal fermentation uses two types of microbes; lactic acid bacteria and cellulolytic bacteria. Selection of the best lactic acid bacteria and cellulolytic bacteria with clear zone testing. Isolates in the clear-zone test were isolates of lactic acid bacteria 478, 504, 508, 520, 525, 540, 543, 546, 548 and cellulolytic bacteria isolates A6, A11, A12. Clear cut test results obtained the best isolates 478 and A11 which are used as fermented cereal jelly inoculum. Jali cereals were fermented with four treatments: control, addition of BAL inoculum, addition of cellulolytic bacterial inoculum and addition of BAL mixed inocula and cellulolytic bacteria. Jali cereal fermentation was carried out for two days and sampling was performed to determine the activity of bacterial growth during fermentation, pH and temperature. The fermented Jali cereal is then dried and made flour. Proximate analysis was performed on Jali flour. The results showed that the addition of bacterial cellulolytic inoculum had a higher protein content of 11.64% compared to other treatments, BAL 7, 43%, BAL + BS 6.26% and 5.08% control. Microscopic analysis was performed to determine the effect of fermentation on starch granules. The use of bacterial inoculum in the manufacture of Jali fermented flour can improve the quality and quantity of nutrient content.Keywords: lactic acid bacteria, cellulolytic bacteria, fermentation, Jali cereals.
Deteksi Keragaman Genotip Hibrid Ikan Lele Sangkuriang, Mutiara Transgenik dan Non Transgenik Pada Keturunan Pertama Buwono, Ibnu Dwi; Lathifah, Asri Ulfah; Subhan, Ujang
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.595 KB) | DOI: 10.14203/jbi.v14i1.3671

Abstract

ABSTRACTThe genotypic diversity showed by the hybrid crossbreed of transgenic Mutiara (carrying African catfish growth hormone) with non-transgenic catfish (Mutiara or Sangkuriang strain) high enough (many polymorphic fragments) in the first offspring. The aims of the study were to detect genotypic diversity from Sangkuriang catfish, transgenic Mutiara, non-transgenic Mutiara and first offspring (hybrid F1) with RAPD (Random Amplified Polymorphic DNA) marker using 2 primary types (OPA-03 and OPA-06). Experimental method is exploratively used in this research with qualitative descriptive analysis. The amplification results show the OPA-03 primer (5-AGTCAGCCAC-3 ) is the best primer that visualizes fragments (polymorphic and monomorphic) in all samples. The genetic relationship of the test fish using the NTSYSpc program shows that the OPA-03 phenogram in the first progeny of crossing the transgenic Mutiara male and Sangkuriang female has more genotypic diversity than other crosses. The first offspring of the broodstock crosses of the same strain (Sangkuriang and Sangkuriang) had a kinship of 70%, the crosses between non-transgenic Mutiara with Sangkuriang had 79% genetic similarity. The highest genetic similarity index (82%) was obtained from the first progeny of crossing transgenic Mutiara with Sangkuriang.Keywords: polymorphism, RAPD, phenogram, crossing, transgenesis
Seleksi Konsorsium Mikroorganisme untuk Pembenah Lumpur Sidoarjo sebagai Media Tanam Pikoli, Megga Ratnasari; Setyawati, Adi Puji; Larasati, Tri Retno Dyah; Mulyana, Nana
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.82 KB) | DOI: 10.14203/jbi.v14i1.3660

Abstract

ABSTRACTThe mud that erupted in Sidoarjo, East Java, has been known from several studies can be used as plant growth media. However, its ability has limitations, particularly due to the very high heavy metal content and phenol will gradually poison the plants. Therefore, a study has been conducted in order to obtain a consortium of microorganisms which have ability to support plants growth and remediate the mud so that further can be used as a growth media. The selected microorganisms consist of Azotobacter sp. KDB2, Bacillus sp. KLBN1, Bacillus sp. BMC4, Pseudomonas sp. BMC6, Trichoderma sp. PJF6 and Trichoderma sp. PJF7F7, which were combined in five consortia, namely K1, K2, K3, K4, and K5. The selection was performed on the ability to produce indole acetic acid, dissolve phosphate, decrease phenol concentration, and decrease the concentration of Pb and Cd ion metals. The results showed that the five consortia have capacity in the five parameters examined. The ability to remove Pb by microbial consortia which consist of Azotobacter, Bacillus, Pseudomonas, and Trichoderma is a new information of the role of microorganism on heavy metal removal. The existence of positive and negative interactions within each consortium lead to differences in the kind of superior consortium generated from each parameter. The consortium K4 showed advantages in 3 parameters, that were in the indole acetic acid production and the Pb and Cd decreases, while the other consortia showed the best capacity only on one of the parameter examined. Therefore, the selected consortium recommended for fixing the Sidoarjo mud is K4, which consists of Azotobacter sp., Bacillus sp. BMC4, Pseudomonas sp. BMC6, Trichoderma sp. PJF6 dan Trichoderma sp. PJF7.Keywords: biofertilizer, bioremediation, Azotobacter, Bacillus, Pseudomonas, Trichoderma
Induksi Poliploidi pada Tanaman Talas (Colocasia esculenta (L.) Schott) Kultivar Kaliurang dengan Perlakuan Kolkisin secara In Vitro Ermayanti, Tri Muji; Wijayanta, Ardian Nur; Ratnadewi, Diah
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (708.917 KB) | DOI: 10.14203/jbi.v14i1.3667

Abstract

ABSTRACTGenetic modification to increase productivity and other better growth characteristics of Kaliurang taro could be done by various methods; one of them is polyploid induction. Kaliurang taro has performed excellent traits, it is resistant to leaf blight disease and pests. Polyploid plants of Kaliurang taro are expected to have excellent char-acteristics and increased productivity. The objective to this study was to obtain an efficient method through in vitro induction of polyploidy using colchicine on Kaliurang taro. Aseptic plantlets of Kaliurang taro were soaked in colchicine solution at 0.05, 0.1 and 0.2% for 1, 2 and 3 days. Control was untreated plantlets. Each treatment consisted of 12 replicates. The colchicine-treated plantlets were then planted on MS medium containing 2 mg/L BAP, 1 mg/L thiamine and 2 mg/L adenine. Survival rate and vegetative growth of plantlets were observed every week for 8 weeks after planting. The results showed that increasing colchicine concentra-tion and the soaking period produced planlets with various survival rate. Flowcytometric analysis indicated that the treatment of soaking the plantlets in 0.05% colchicine for 1 day resulted in 14.3% of tetraploid plantlets. The most efficient of colchicine treatment was 0.2% for 3 days, resulting in 57.1% tetraploids, with the efficient value of 33.3%. There was chromosome multiplication from diploid to tetraploid which was confirmed through both flowcytometric analysis and chromosomes counting.Keywords: Taro (Colocasia esculenta L.), Kaliurang, in vitro, flowcytometer, chromosome multiplication, tetraploids

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