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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 17 Documents
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Search results for , issue " Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA" : 17 Documents clear
Perubahan Persentase Unsur Hara Serasah Akibat Proses Dekomposisi Pada Empat Spesies Tanaman Gugur Daun di Kebun Raya Purwodadi Darmayanti, Agung Sri; Rindyastuti, Ridesti
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3065

Abstract

Percentage changes of litter nutrient due to decomposition process of four deciduous plant species in Purwodadi Botanic Garden. The nutrient release pattern of plant litter is different among species. Decomposition and nutrition release of organic material was observed in four species Swietenia macrophylla, Lagerstroemia speciosa, Ficus benjamina and Kigelia Africana in Purwodadi Botanic Garden. Litter mass was measured and chemical compositionwas analized including poliphenol, celulose, lignin, carbon (C), Nitrogen (N), C/N ratio, phospor (P), and Kalium (K) during 3 months. Litterbag method was applied in this research. Freshlitters were brought into bag and placed in the forest floor. Litterbag and litter chemical monitoring was established once a week. The research result showed that C ratio of four species in early phase increase then decrease in late phase of incubation and significantlydifferent from initial phase except in F. benjamina. N ratio decreased in first month and increases until late phase of incubation. C/N ratio increase in early phase then significantly decreasesuntil 3 months incubation. P ratio tends to decrease in first and third month. Ratio of polyphenol and Lignin decreases, however celluloses increase. The litter of four species has low chemical quality, respectively have low C/N ratio (< 25 %), lignin < 15 %, poliphenol < 3 % and (L+Pp)/ N > 8 %. Consequently, the litter tends to immobilize than mineralize. It effected to high ratio ofN such in L. speciosa. Decreasing and releasing of N in K. Africana showed that its nutrient transfer is better than in other species. P ratio quickly decreases in first 4 weeks of decomposition, it showed that P is not a limiting factors in species decomposition, relatively.Keywords : Litter, decomposition, percentage changes, nutrient
Peroksidasi Lipid oleh Parasetamol dan Ekstrak Air Panas Teh Hijau (Camellia sisnensis ) pada Sel Khamir Candida tropicalis yang di Simpan pada Suhu Rendah Julistiono, Heddy
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3070

Abstract

ABSTRACTLipid Peroxidation by Paracetamol and Hot Water Extract of Green Tea (Camellia sinensis ) in Yeast Candida tropicalis preserved in Low Temperature. The use of C. tropicalis cell as a tool to evaluate antioxidant property of green tea to protect oxidative stress caused by paracetamol in cell level was investigated in our laboratory. Immediate availability of cell culture will significantly reduce the time of cell preparation. Low temperature preservation of cell culture is one of methods to produce cell cultures. However, low temperature might affectphysiological state of the cell. In this study, effect of low temperature preservation (4 ºC, 10 days) on the oxidative response of yeast cell treated with paracetamol and hot water extract of green tea had been investigated. Cells incubation with paracetamol 0.3 % for 2 h caused oxidative stress in both fresh and preserved culture since the content of a marker of oxidativestress, peroxyd lipids increased significantly. Whereas, concentration of peroxidised lipids in preserved cultures was lower than that of fresh culture. Increasing of peroxydized lipids followed with decreasing of cell viability in fresh culture but not in preserved culture. Green tea withconcentration of 0.1 % decreased peroxyd lipids in fresh cultures treated with paracetamol but not in preserved cultures. Green tea with concentration of 0.2 % or 0.4 % in increased peroxyd lipids in fresh cultures treated with paracetamol but not in preserved cultures. The data indicated that green tea did not show anti- or pro-oxidative effect in cultures preserved in low temperature treated with paracetamol. However, induction of super oxide dismutase (SOD), an antioxidant defense enzyme, was not observed in cell preserved in low temperature. The study revealedthat low temperature might make the cell more resistant against prooxidative properties of paracetamol.Keywords: C. tropicalis, oxidative stress, low temperature, paracetamol, green tea
Iradiasi Sinar ? pada Biak Tunas Kentang Hitam (Solanostemon rotundifolius) Efektif untuk Menghasilkan Mutan Witjaksono, Witjaksono; Leksonowati, Aryani
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (608.916 KB) | DOI: 10.14203/jbi.v8i1.3075

Abstract

Irradiation of ??ray at shoot culture of Kentang hitam (Solanostemon rotundifolius) is effective for mutant production. Kentang hitam is sterile and vegetatively propagated andtherefore its genetic diversity is narrow. Mutation is an alternative way to increase genetic heterogeneity. Irradiation of shoot cultures with different doses followed by culturing of the inoculum (leaf, petiole and internodes) from that irradiated culture on a regeneration medium MS containing 5 mg/l BA and 0.1 mg/l NAA has resulted in curvilinear respon of growth, morphogenesis and plant regeneration. The growth variables increase from 0 to 6 Gy and thendecrease to 25 and plateu or increase a little at 35 Gy and growth death wes observed at 50 Gy. Leaf and petiole inocula were more responsive than the internode. Respon of growth of shootregeneration of 50% were obtained at doses of 10-12.5 Gy. However higher level of doses, such as 25 Gy had also been effective for inducing mutant. Morphological and growth different were observed from growth in tissue culture to the field. Mutants were recovered, forexample, the one with early flowering.Keywords : Solanostemon rotundifolius, Irradiation, gammarays, cultur in vitro, mutan
Dampak Kegiatan Manusia Terhadap Keragaman dan Pola Distribusi Kumbang Sungut Panjang (Coleoptera: Cerambycidae) di Gunung Salak, Jawa Barat Noerdjito, Woro Anggraitonongsih
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3066

Abstract

The impact of human activities on the Longhorn Bettles Diversity and Their Distribution Patterns in Mount Salak, West Java, had been conducted by observation and collecting efforts on various types of habitats which include undisturbed and disturbed forests. Longhornbeetles are one of the largest groups of wood borers and therefore typical forest dependent insects. The species diversity and distribution pattern of these beetles may vary with speciescomposition age of the trees, and stability of the forest, and therefore they are a useful bioindicator for assessing forest condition. The beetles were collected using “Artocarpus branchtraps”, a bait trap. It is a bundle of about 5-6 freshly-cut branches of Artocarpus heterophyllus (jackfruit), about 80 cm long with many leaves, tied up and hung from a tree trunk or standingpoles at 1.5 m above the ground. The trap attracted longhorn beetles that need feeding for reproductive maturity. Ten traps were set at each study site (habitat). The attracted beetleswere collected every week by beating method, because in West Java forest area, beetles are usually gathered by the 6th day after trap setting. Light traps were also set up for 2 night, at each habitat for collecting nocturnal longhorn beetles. Totally 578 specimens were collected, consisted 38 species of longhorn beetles, dominated by small body size species less than 10mm. Out of these 38 species, some species were known distributed in certain habitat andelevation, other species have wider distribution, where as some species were endemic and new record for Java.Keywords: distribution, diversity, longhorn beetles, Mount Salak
Reproduksi Ikan Endemik Butini (Glossogobius matanensis Weber 1913) Berdasarkan Kedalaman dan Waktu di Danau Towuti, Sulawesi Selatan Mamangkey, Jefry Jack; Nasution, Syahroma Husni
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1839.854 KB) | DOI: 10.14203/jbi.v8i1.3064

Abstract

Reproduction of Endemic Fish Butini (Glossogobius matanensis) Based on Depth and Time in Towuti Lake. Butini fish (Glossogobius matanensis) belonging to the Gobiidae family and is one ofendemic fish in Towuti Lake and classified as demersal fish. Fishing activities and habitat quality changes tend to reduce butini stocks in Towuti Lake. Therefore, it needs to be protected of stocksfor sustainability. This research is to determine reproduction of endemic fish based on depth and time as basic data for the effort of conservation and domestication. Gonad maturity that was analyzed histologically obtained five maturity stages. Based on the diversity of egg size, especially in the gonad maturity stage (IV), it is indicated that butini fish are classified as partial spawner. Male and female fish gonad mature (50%) at 36.22 and 31.43 cm total length, respectively. October and March, an estimated spawning peak of butini fish in Towuti Lake held this month at a depth of 150m and 200 m. Fecundity of butini fish in gonad maturity stage (III and IV) between 11184-66305 eggs and 14922-81522 eggs, respectively. The highest mean of fecundity there in October (56,907 eggs) and the lowest was found in July (25,447 eggs). Fecundity of fish at 25 m-200 m depths ranging from 30.778-35.329 eggs.Keywords: Reproduction, endemic fish, Glossogobius matanensis, depth and time, Towuti Lake
Keragaman Genetik Rusa Sambar (Rusa unicolor), Pemanfaatan dan Implikasinya Untuk Konservasi Wirdateti, Wirdateti
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (546.385 KB) | DOI: 10.14203/jbi.v8i1.3071

Abstract

Genetic Diversity of Sambar Deer (Rusa unicolor) in Utilization and Implications for Conservation. The deer sambar (Rusa unicolor) is one of species of Genus Rusa, was widely distributed in South Asia antil South East Asia. This species in Indonesia was widely distributed in Kalimantan and Sumatra island, but now, the hunting, poaching and habitat loss have reduced its populations drasstically. In order to provide useful information for its conservations. The genetic diversity and population structure of the wild sambar deer was observed by analyzing the 962 bp long of fragment mitochondrial DNA (mtDNA) control region (D-loop)fragment. The result detected, twenty different haplotypes from 22 samples from Borneo and Sumatra. Overall, sambar deer have a relatively high genetic diversity compared to other the ceervid species, with a haplotype diversity (h) 0.9870 and nucleotide diversity (ð) 2.931±0.260%.The genetic distance of Borneo populations (East Borneo) higher compared with Sumatra’s populations that is d = 0.018±0.003 on Borneo and d = 0.009±0.002 on Sumatra. The structure of phylogenetic tree showed that two populations were separate based on the haplotypes differences.Keywords: conservation, control region, genetic diversity, sambar deer.
Anthropogenic Influences on The Sosioecology of Long-Tailed Macaques (Macaca fascicularis) in Lombok Island, Indonesia Hadi, Islamul; Suryobroto, Bambang; Watanabe, Kunio
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.427 KB) | DOI: 10.14203/jbi.v8i1.3061

Abstract

Beberapa Pengaruh Antropogenik pada Sosioekologi pada Monyet ekor Panjang (Macaca fascicularis) di Pulau Lombok, Indonesia. Survey distribusi monyet ekor panjang dilakukan di P. Lombok selama 2001-2009 dan dari 37 kelompok, satu kelompok jantan muda dan 3 individu soliter yang terdata dari 27 lokasi sekitar 63% monyet dijumpai pada areal karena terpengaruh oleh aktivitaskehidupan manusia, seperti hutan sekunder, kebun buah-buahan, hutan persembahan dan tempat rekreasi. Besarnya kelompok monyet cenderung menjadi lebih besar di area semi buatan dibandingkan dengan kelompok yang hidup liar (Liar= 7.08, buatan =19.04, X2 = 5.4763, df = 1, P= 0.01928). Faktor-faktor ketergantungan pada manusia yang menyediakan sejumlah pakan ternyata mempengaruhi tingkah lakunya sedangkan penebangan hutan dan pengubahan alih fungsi menjadi kawasan lain selain hutan akan memicu monyet terkesan menjadi hama tanaman.Kata Kunci: Monyet kra, Macaca fascicularis, Pulau Lombok, Anthropogenik
Persilangan Pisang Liar Diploid Musa acuminata Colla var malaccensis (RIDL.) Nasution Sebagai Sumber Polen dengan Pisang Madu Tetraploid Poerba, Yuyu S.; Ahmad, Fajarudin; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.794 KB) | DOI: 10.14203/jbi.v8i1.3076

Abstract

Hybridization of wild diploid Musa acuminata Colla var malaccensis (Ridl.) Nasution as pollen source with teraploid Pisang Madu. Indonesia and South East Asia is the center of origin and genetic variability of bananas, specifically species Musa acuminata Colla. At least 15 varieties of wild Musa acuminata are found in Indonesia. Some of them have resistances for several diseases of bananas. One approach in banana breeding program is hybridizingbetween tetraploid parent and diploid parent of banana. This research was aimed to produce new triploid banana hybrid by crosses between tetraploid female parent ‘Pisang Madu’ and wild diploid male parent Musa acuminata Colla var malaccensis (Ridl.) Nasution. From 302 crosses, 53.9% of crosses produced seeds. However, only 48.46% of seeds contained embryos, and only 1.27% of embryos grew and developed. Bunch weight and leaf length of hybridplants were not significantly different with female parent, tetraploid Pisang Madu. Plant height of the hybrids was not significantly different with male parent, wild diploid Musa acuminatavar malaccensis. Pseudostem diameter and leaf width of the hybrid plants were between female tetraploid parent and male wild diploid parent. RAPD profiles (DNA bands) of the hybrids were originated from the female parent, male parent and both parents were 38.46, 34.6, and 26.92%, respectively. The hybrids were confirmed to be triploids (3x=33).Keywords: banana, breeding, hybrids, tetraploid, wild diploid, Pisang Madu, Musa acuminata Colla var malaccensis (Ridl.) Nasution
Pelestarian Lingkungan Berbasis Kepercayaan Lokal dan Upacara Tradisi: Studi Kasus Masyarakat di Sekitar Gunung Salak Royyani, Mohammad Fathi; Walujo, Eko Baroto
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (73.318 KB) | DOI: 10.14203/jbi.v8i1.3067

Abstract

Mount Salak in West Java has important position as water catcment area of water, rain forest, and bank of biodiversity. To protect and conserve this area, Indonesian goverment through the Forest Department develop has Mount Salak to be National Park. Local people who live surrounding Mount Salak have local wisdoms about the benefit of plant and local perspective to manage biodiversity. For local people, Mount Salak is not only to their daily needs but also to save their history and legend about their ancestor. To collect data, we conducted indepth interview with local peple, observed and involved with their ritual local people of Mount Salak have ritual tradition and local belief about Mount Salak, that keep Mount Salak area protected and conserved sustainability.Keywords: Biodiversity, Gunung Salak, Kepercayaan Lokal, Konservasi, Upacara tradisi,
Apis koschevnikovi: Distribution in South Kalimantan and Cytochrome b Mitochondrial DNA Variations Fitriya, Jazirotul; Raffiudin, Rika; Atmowidi, Tri; Hepburn, Randall
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.79 KB) | DOI: 10.14203/jbi.v8i1.3063

Abstract

Apis koschevnikovi: Distibusi di Kalimantan Selatan dan Variasinya pada Mitokondria Cytochrome b. Variasi mitokondria berdasarkan Cytochrome b (Cyt b) pada Apis koschevnikovi di lakukan di tujuh lokasi di Kalimantan Selatan Indonesia. Di koleksi 29 koloni A. koschevnikovi yang diperoleh di kawasan hutan primer dan sebagian koloni tidak dijumpai dihutan bekas penebangan. Hasil analisis Cyt b juga mengindikasikan bahwa terjadi variasi genetik dari jenisini walaupun secara morfologi menunjukkan bentuk yang samaKeywords : Apis koschevnikovi, penebangan hutan, variasi haplotipe

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