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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 18 Documents
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Search results for , issue "Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA" : 18 Documents clear
Studi Kelimpahan Aktinomisetes Tanah dan Hubungannya Terhadap Enzim Selulase, Amilase, Total Karbon dan Nitrogen Hutan Pasca Kebakaran Bukit Bangkirai Kalimantan Timur Nurkanto, Arif
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.489 KB) | DOI: 10.14203/jbi.v5i1.3208

Abstract

ABSTRACTSoil Actinomycetes Population, Enzymes Activity, and its Relation with Carbon and NitrogenContent, in Bukit Bangkirai, East Kalimantan. Bukit Bangkirai is one of the tropical forest inIndonesia have been exposed with intense forest fire. The affected forest is subjectivelydivided into three level of damages, heavily damage forest (HD), low damage forest (LD) andcontrol (K). The objective of this research was to observe the abundance of Actinomyceteswhich have important role in ecological process. Through decompotition of organic materialsand nutriens cycle. Actinomycetes were isolated and enumerated by SDS-YE method. CFU/ gsoil (x 104) in K, HD and LD are 41,86 ± 25,52, 16,09 ± 5,70 and 18,96 ± 4,19 respectively. Amylaseand cellulase were determined by DNS method. Carbon and Nitrogen total were determinatedby CN analyzer. The different of amylase, cellulase activities and abundace of Actinomycetesbetween HD, LD, and Control plot were not significant. However, carbon and nitrogen totalare different. LD plot has the highest carbon and nitrogen total, followed by control and HDplot. There has no significant different among plot observed may indicate microbial communitiesof soil in Bukit Bangkirai have been recovered.Key words : actinomycetes, fire forest, amylase, cellulase, nitrogen total, carbon total
Ekstrasi Senyawa Antibakteri Dari Diatom Chaetoceros gracilis dengan Berbagai Metode Setyaningsih, Iriani; Hardjito, Linawati; Monintja, Daniel R.; Sondita, M. Fedi A.; Bintang, Maria; Lailati, Nispi; Panggabean, Lily
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.24 KB) | DOI: 10.14203/jbi.v5i1.3203

Abstract

ABSTRACTExtraction of Antibacterial Compound from Diatom Chaetoceros gracilis WithDifferent Methods. Diatom is phytoplankton which is commonly found in off shore.Chaetoceros produce antibacterial which inhibit some bacteria. This research was donein 3 steps. At first, Chaetoceros gracilis was cultivated in temperate controlled roomwith lighting 24 hours. The culture was harvested on 14 days, then it was dried andweighted. The second step, biomass was disrupted by different method such as sonicator,glass beads, and undisrupting. Then the biomass was maserated, filtrated, and evaporated.The crude extracts were tested to pathogen bacterial. The third step, the extraction wasconducted using hexana (non polar solvent), ethyl acetate (semi polar solvent), andmethanol (polar solvent). The crude extracts were tested to the pathogenic bacteria.The result showed that the produce cell disruption antibacterial activity by sonicator.The biggest inhibition zone was obtained by hexana but produced lower yield.Key words: Chaetoceros gracilis, growth, extraction, disrupting, antibacteria
Distribusi Spasial dan Temporal Ikan Bonti-bonti (Paratherina striata Aurich), Endemik di Danau Towuti-Sulawesi Selatan Nasution, Syahroma Husni
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.76 KB) | DOI: 10.14203/jbi.v5i1.3209

Abstract

ABSTRACTSpatial and Temporal Distribution of Bonti-bonti (Paratherina striata) an EndemicFish in Towuti Lake, South Sulawesi. Bonti-bonti (Paratherina striata) is an endemicfish species in Towuti and Mahalona Lake. This fish included into vulnerable species. Itshould be protected from decreasing of fish population due to increasing exploitationand habitat quality changes. The objective of this research to study on spatial and temporaldistribution of the fish, as a basic information for its conservation. Samples were collectedfrom May 2006 to April 2007 using experimental gillnet mesh size 0.625, 0.75, 1.0, and1.25 inches at five stations. Fish number and size captured during 15 hours. Waterquality parameters that analyzed were temperature, conductivity, pH, and dissolvedoxygen using water quality checker-Horiba, alkalinity used titration methode, whilewater level and rain fall obtained from PT. Inco. Distribution of fish abundance wasanalyzed with Mann-Whitney non-parametric test. Water quality parameters thatinfluencing fish abundance were analyzed by multivariate analysis. The results show thatthe distribution of bonti-bonti spreading widely starting from lakeside to middle of thelake. The highest abundance of the fish are in inlet with sand, gravel, and stone substrat.Fish size was obtained more various at the inlet station than other stasions and it predictedas main habitat. Water qualitiy parameters of were not factors influencing difference ofspatial distribution but it was influenced by behavior of habitat selection. The highesttemporal distribution of the fish abundance in November and December influenced bydissolved oxygen and high water level.Key words : Spatial and temporal distribution, Paratherina striata, endemic fish, abundance,Towuti Lake
Seroepidemiologi Nipah Virus pada Kalong dan Ternak Babi di Beberapa Wilayah di Indonesia Sendow, Indrawati; Field, Hume; Adjid, R.M. Abdul; Syafriati, Tatty; Darminto, Darminto; Morrissy, Chris; Daniels, Peter
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.164 KB) | DOI: 10.14203/jbi.v5i1.3205

Abstract

ABSTRACTNipah Virus Seroepidemiology in Flying Fox and Pig Husbandry in Several Areasof Indonesia. Nipah is a dangerous zoonotic disease which was carried by flying fox.The disease had been occurred in Malaysia in 1999 and infect pigs and caused humandeath. Indonesia is adjacent country to Malaysia, hence, a serological study had beenconducted on 156 flying fox (P. vampyrus) sera from North Sumatera, West Java, CentralJava and East Java. Besides that, 2740 pig sera was randomly collected in differentprovinces to detect Nipah infection. Both flying fox and pig sera were tested usingELISA test to detect the presence of Nipah antibody. The results indicated that 37 from156 flying fox sera (23.7%) has antibodies against Nipah virus. Infections were occuredin all sampling sites with the prevalence varied from 18% to 33 %. Meanwhile, no pigsera tested (2740) had antibody against Nipah virus. Based on these results it can beconcluded that Nipah virus infections were occurred in flying fox in some parts inIndonesia, but not in pigs. It was suggested that the presence of Nipah virus in Indonesiashould be anticipated. Hence the distribution of its infection in pigs and human must beanticipated. Monitoring of Nipah infection in areas adjacent to Malaysia must be increasedto detect the entering of the disease in Indonesia.Keywords: Nipah, pigs, flying fox, serology
Kelemahan Gen 12S rRNA Untuk Mempelajari Struktur Populasi Genus Aethalops (Chiroptera: Pteropodidae) di Indonesia: Tanggapan Tulisan Maharadatunkamsi & M. Syamsul Arifin Zein pada Jurnal Biologi Indonesia 4 (2): 75-86. Sutrisno, Hari
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (35.884 KB) | DOI: 10.14203/jbi.v5i1.3210

Abstract

TULISAN PENDEKKelemahan Gen 12S rRNA Untuk Mempelajari Struktur Populasi GenusAethalops (Chiroptera: Pteropodidae) di Indonesia: Tanggapan TulisanMaharadatunkamsi & M. Syamsul Arifin Zein pada Jurnal BiologiIndonesia 4 (2): 75-86.
Parasitoid Chalcidoidea (Insecta: Hymenoptera) Sebagai Agen Pengendalian Hama Secara Biologi Pada Terong (Solanum melongena L.) dan Pare (Momordica charantia L.) Sofyan, Muhamad Rofik; Ubaidillah, Rosichon
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i1.3206

Abstract

ABSTRACTThe Parasitics wasp superfamily Chalcidoidea (Insecta : Hymenoptera) potentialas biological control agents for pests of Eggplant (Solanum melongena) and BitterMelon (Momordica charantia). The recent contribution on diversity of parasitics waspChalcidoidea for biological control perspective against the pests of the two economiccrops was discussed, with emphasis on the most significant steps for the selection ofcandidate biological control agents. The survey was carried out in Bogor, West Javausing Malaise traps, Yellow pan traps and a Swept net. The crops are the most economicallyimportant in agriculture not only in West Java but also in Indonesia. Meanwhile the pestcontrol of those two crops has so far been used only by the insecticides and would affectto the health of the production of the crops when the vegetables are consumed freshly. Ofthe 78 species within 8 familes of Chalcidoidea are found in both crops, 7 species can beclassified as potential biological control agents. With the object of biological control ofthese 7 species, a survey was carried out to establish the presence and importance oftheir natural enemies on those crops in Java.Key words : Bitter Melon, Chalcidoidea, Eggplant, Parasitoids, Pest control.
Karakter Pertumbuhan dan Aktivitas Nitrifikasi Kultur Mikroba N-Sw Agustiyani, Dwi; Imamuddin, Hartati; Haryanto, Tanto
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.815 KB) | DOI: 10.14203/jbi.v5i1.3211

Abstract

ABSTRACTThe growth character and nitrification activity of nitrifying cultures (N-Sw). Theculture of nitrifiers (N-Sw) was obtained from acclimated sludge of sawit palm oil industrywastewater. The growth and nitrification activity of those cultures were investigated.The result shows that the growth and nitrification activity attained optimum at pH 7-8,and temperature of 30 0C. The culture of nitrifiers was still growing at pH 5, but thenitrification activity was not detected. The growth of nitrifiers and the nitrification activitywas inhibited at 40 0C. The ammonium conversion rate reached 0,088-0,090 mg NNH4+/L/hour/g biomass. The rate of ammonium conversion in the bioreactor increasedto 0.630 mg N-NH4+/L/hour/g biomass as the pH maintained at 7.5-8 and dissolvedoxygen at 3-4 mg/l O2.Key words: nitrifying culture, ammonium, ammonium convertion rate
Deletion Analysis of a Xylanase Gene from Fibrobacter succinogenes S 85 and Its Expression in Escherichia coli HB 101 Suryadi, Y.
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.004 KB) | DOI: 10.14203/jbi.v5i1.3207

Abstract

ABSTRAKAnalisis Delesi Gen Xilanase Asal Fibrobacter succinogenes S 85 dan Ekspresinyapada Escherichia coli HB 101. Plasmid pBX6 dengan sisipan DNA berukuran 3 Kb asalDNA genomik Fibrobacter succinogenes S 85, menyandi aktifitas xilanolitik (mampumendegradasi substrat xilan) bila ditumbuhkan dalam vektor E. coli HB 101. Tujuanpenelitian ini adalah untuk mengidentifikasi lokasi fragmen DNA yang berperan dalammenentukan aktifitas pBX6 menggunakan analisis delesi. Suatu seri analisis delesi terhadappBX6 telah dilakukan dengan menghilangkan beberapa fragmen DNA dengan cara kloninghasil restriksi enzim maupun PCR. Satu fragmen delesi asal produk PCR berukuran 0.7Kb di sub kloning menggunakan cloning kit pCR TOPO dan aktifitas xilanolitiknya ditapislebih lanjut dengan menumbuhkan dalam media agar oat spelt xylan-Remazol brilliantblue (RBB) ditambah antibiotik ampicilin (50 ?g/ml). Transforman positif selanjutnyadiekstraksi dan dikarakterisasi. Hasil penelitian menunjukkan klon yang telah terpotongkehilangan sebagian besar aktifitas xilanolitiknya dibanding klon asal, bila diuji pada kondisiyang sama untuk menghidrolisis substrat xilan. Klon plasmid rekombinan yang telahterpotong menunjukkan aktifitas spesifik sebesar 41,29 + 0,025 U/mg.Key word: Delesi, F. succinogenes S 85; E. coli HB 101, xilanase
Efek Penghambatan Terhadap Pertumbuhan Tumor Paru dan Uji Ketoksikan Akut Ekstrak Kapsul Chang Sheuw Tian Ran Ling Yao Pada Mencit (Mus musculus) dan Tikus (Ratus tanezumi) Fudholi, Ahmad; Meiyanto, Edy; Donatus, Imono Argo; Nurrochmad, Arief; Hakim, Arief Rahman; Murwanti, Retno
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (382.692 KB) | DOI: 10.14203/jbi.v5i1.3202

Abstract

Inhibitory Phases Effect of The Lung Cancer And Acute Toxicity of Chang Sheuw Tian RanLing Yao Capsule Extracts in House mice (Mus musculus) and Rat (Ratus tanezumi). Effortsto find anticancer agents have been developed nowadays, some of them are focused in traditionalherbs. One of the available products in the market that claims effective to cure cancer isthe Chang Sheuw Tian Ran Ling Yao, PT. Daun Teratai extract containing CAPSULE (CSTRLYextract). The aim of this study is to examine of confession of some people which are usingthe useful of medicine CSTRLY extract capsule through inhibitor laboratory effect of theCSTRLY extract in the initiation and post initiation phases of the lung cancer in mice and ratsthat had been induced by eather Benzo[?]pyrene (BP) or Dimetilbenz[?]antrazene (DMBA)and to clarify the potency of acute toxicity and specific toxic manifestations of thephytopharmaca.The results showed that the CSTRLY extract can reduce the cancer incidence caused bycarcinogen, BP and DMBA. Moreover, the extract can also inhibit the cancer growth in themice and rats, especially in the early post-initiation phase. Further, the histopathologicalevaluation showed that up to the highest dose level that technically could be administrated tothe animals (12500 mg/kg bw), no animal death was occurred. Furthermore, the ADG values formale and female rats indicated no significant different (P > 0.05) that relative to the controlgroup. No animals were shows physical symptom as a toxic manifestation. It’s indicated thatthe phytopharmaca no influenced to somatomotor and nervous system. Within the dose rangeadministrations, no detectable morphological toxic effects or histophatological changes of theliver, spleen, heart, and lungs were observed. the acute toxicity value of Chang Sheuw TianRan Ling Yao Capsule was very low (or minimal almost non-toxic with LD50 > 12500 mg/kg bw)and the spectrum of toxic effects of the phytopharmaca were considered negligible.Key words: Ekstract, CSTRLY, mice and rat, BP, DMBA, carsinogenesis, lung cancer
DISTRIBUSI SPASIAL DAN TEMPORAL IKAN BONTI-BONTI (PARATHERINA STRIATA AURICH), ENDEMIK DI DANAU TOWUTI-SULAWESI SELATAN Nasution, Syahroma Husni
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i1.3209

Abstract

ABSTRACTSpatial and Temporal Distribution of Bonti-bonti (Paratherina striata) an EndemicFish in Towuti Lake, South Sulawesi. Bonti-bonti (Paratherina striata) is an endemicfish species in Towuti and Mahalona Lake. This fish included into vulnerable species. Itshould be protected from decreasing of fish population due to increasing exploitationand habitat quality changes. The objective of this research to study on spatial and temporaldistribution of the fish, as a basic information for its conservation. Samples were collectedfrom May 2006 to April 2007 using experimental gillnet mesh size 0.625, 0.75, 1.0, and1.25 inches at five stations. Fish number and size captured during 15 hours. Waterquality parameters that analyzed were temperature, conductivity, pH, and dissolvedoxygen using water quality checker-Horiba, alkalinity used titration methode, whilewater level and rain fall obtained from PT. Inco. Distribution of fish abundance wasanalyzed with Mann-Whitney non-parametric test. Water quality parameters thatinfluencing fish abundance were analyzed by multivariate analysis. The results show thatthe distribution of bonti-bonti spreading widely starting from lakeside to middle of thelake. The highest abundance of the fish are in inlet with sand, gravel, and stone substrat.Fish size was obtained more various at the inlet station than other stasions and it predictedas main habitat. Water qualitiy parameters of were not factors influencing difference ofspatial distribution but it was influenced by behavior of habitat selection. The highesttemporal distribution of the fish abundance in November and December influenced bydissolved oxygen and high water level.Key words : Spatial and temporal distribution, Paratherina striata, endemic fish, abundance,Towuti Lake

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