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All Journal JBIO: jurnal biosains (the journal of biosciences) Saintia Biologi IJOEP : International Journal of Ecophysiology
Nunuk Priyani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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POTENSI EKSTRAK UMBI BAWANG LOKIO (Allium chinense G. Don) DALAM MENGHAMBAT Escherichia coli DAN MENINGKATKAN MASA SIMPAN IKAN NILA (Oreochromis niloticus L.) Grace Emalia Masniari Lumbantoruan; Nunuk Priyani; It Jamilah
JBIO: jurnal biosains (the journal of biosciences) Vol 2, No 3 (2016): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v2i3.4957

Abstract

Bawang Lokio (Allium chinense G. Don) banyak tumbuh di Sumatera Utara, Indonesia, sehingga disebut “Bawang Batak” oleh suku Batak. Kandungan senyawa alami dari tanaman ini dalam pengawetan makanan segar belum banyak dilaporkan. Penelitian ini bertujuan untuk menentukan konsentrasi hambat minimum dari ekstrak etanol umbi bawang Lokio dalam menghambat bakteri Escherichia coli yang diisolasi dari ikan Nila dan meningkatkan masa simpan ikan Nila. Aktivitas antimikroba diuji menggunakan metode difusi cakram dengan konsentrasi 0, 7.5, 10, 20, 30, 40 dan 50% (b/v). Untuk uji pengawetan ikan segar, ikan Nila yang telah disiangi, direndam dalam ekstrak etanol umbi bawang Lokio konsentrasi 100%, disimpan selama 36 jam pada suhu ambient (27 °C) dan kulkas (4 °C) dengan waktu pengamatan setiap interval 6 jam. Parameter pengamatan ialah perubahan organoleptik, Angka Lempeng Total (ALT) bakteri dan Total Volatile Base Nitrogen (TVBN). Konsentrasi Hambat Minimum (KHM) dari ekstrak etanol umbi bawang Lokio adalah 10% dengan kategori sedang terhadap E. coli. Berdasarkan Standar Nasional Indonesia (SNI), nilai organoleptik ikan Nila dapat diterima hingga penyimpanan jam ke-30, sementara nilai ALT dan TVBN sampai jam ke-18. Hal ini berbeda secara signifikan dengan kontrol negatif yang memberikan kelayakan ikan hanya sampai 6 jam. Perbedaan aktivitas senyawa antimikroba pada konsentrasi yang berbeda dan suhu penyimpanan menjadi penyebab turunnya mutu ikan. Dapat disimpulkan bahwa ekstrak etanol umbi bawang Lokio menghambat E. coli pada konsentrasi hambat minimum 10% serta masa simpan ikan Nila maksimal adalah 18 jam pada suhu ambient.   Kata Kunci: Bawang Lokio, ikan Nila, masa simpan, Sumatera Utara.
POTENSI TANAMAN ORNAMENTAL (Aglaonema sp., Dieffenbachia sp., dan Spathiphyllum sp.) DALAM MENURUNKAN JUMLAH BIOAEROSOL Sisca Teresia; It Jamilah; Nunuk Priyani
JBIO: jurnal biosains (the journal of biosciences) Vol 2, No 3 (2016): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v2i3.4958

Abstract

Bioaerosol adalah partikel debu yang terdiri atas bakteri dan jamur beserta spora lainnya yang mampu bertahan hidup dalam ruangan ketika tingkat suhu dan kelembaban yang memadai. Keberadaannya diruangan dalam batas tertentu tidak berbahaya, namun sewaktu-waktu dapat mengganggu kesehatan. Penelitian ini bertujuan untuk mengetahui pengaruh tanaman ornamental Aglaonema sp., Dieffenbanchia sp. dan Spathiphyllum sp. dalam mengurangi jumlah koloni bakteri dan jamur dalam ruangan. Isolasi mikrob bioaerosol dilakukan dengan metode air sampling dengan tiga kali ulangan sebelum dan setelah tiga jenis tanaman diletakkan pada tiga ruangan kelas yang berbeda. Tanaman Aglaonema sp. memiliki potensi paling efektif dalam menurunkan jumlah bakteri aerosol dari minggu pertama hingga minggu ke tiga, sedangkan untuk koloni jamur tidak menunjukkan adanya penurunan. Jumlah koloni bioaerosol pada control dalam ruangan masih sesuai dengan baku mutu keputusan Menteri Kesehatan RI tahun 2002. Jenis bakteri yang ditemukan seperti Streptococcus, Staphylococcus, Bacillus, Pseudomonas dan Shigella, sedangkan jenis jamur yang ditemukan seperti Aspergillus, Penicillium, dan Neurospora.   Kata Kunci: Bioaerosol, Ruang kelas, Aglaonema sp., Spathiphyllum sp.,  Dieffenbanchia sp.
ISOLASI DAN UJI POTENSI BAKTERI TANAH PERTANIAN BERASTAGI SUMATERA UTARA DALAM MENDEGRADASI FUNGISIDA ANTRACOL BERBAHAN AKTIF PROPINEB Diah Sri Utami; Nunuk Priyani; Erman Munir
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.671 KB)

Abstract

Isolation and potential Berastagi agricultural soil bacteria, North Sumatra in degrading propineb-based antracol fungicide has been conducted. The isolated bacteria were grown on Bushnell Hass Broth (BHB) containing 2% of propineb-based antracol fungicide with propineb as the active compound. The cultures have been grown on shaking incubator at 150 rpm for 21 days. The media BHB containing 2% of propineb-based antracol fungicide without bacteria was used as a control. The parameters observed were the growth bacterial, biosurfactant activity, biosurfactant concentration and the residues of propineb which were observed on day 0th, 7th, 14th and 21th. A total of sixteen bacterial isolates were isolated using selective media Bushnel Hass Agar (BHA) containing 2% propineb-based antracol fungicide​​. Two bacterial isolates which were CBA 02 and JBA 04 were selected for further test to determine their ability to degrade propineb. JBA 04 showed much higher ability in reducing propineb concentration up to 60.86%, while CBA 02 was only 5.59% than that of control.   Keywords: biodegradation, bioremediation, biosurfactant, fungicides, propineb
ISOLASI DAN UJI POTENSI ISOLAT BAKTERI PELARUT FOSFAT DAN BAKTERI PENGHASIL HORMON IAA (Indole Acetic Acid) TERHADAP PERTUMBUHAN KEDELAI (Glycine max L.) PADA TANAH KUNING Dessy Merry Silitonga; Nunuk Priyani; Isnaini Nurwahyuni
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.567 KB)

Abstract

Biofertilizer is a safe alternative fertilizer instead of various chemical fertilizers for increasing plant productivity which can minimize the ecological damage. There are plant symbiotic-soil bacteria which can solubilize phosphate or produce IAA. The objectives of this research are to determine the best soil bacteria in producing IAA or solubilizing phosphate and to know their roles in promoting plant growth in unfertile soil. As many as 8 isolates of phosphate solubilizing bacteria and 5 isolates of IAA producing bacteria were found. Among those isolates, there were 2 potential isolates (P2 & P4) that were able to solubilize phosphate and 1 potential isolate (I3) that was able to produce IAA. Isolate P4 produced holozone as wide as 1.45 cm and isolate P2 was 1.3 cm. Meanwhile, isolate I3 produced the highest concentration of IAA compared to the other isolates, that was 33.3 ppm. These isolates were applicated further on unfertile soil (yellow soil) as a medium for soybean growth. Soybeans have been grown for 10 weeks to observe the effect of those potential isolates on plant growth and productivity. The result showed that application of  isolate I3 was able to promote plant growth and increase plant productivity better than the other isolates as well as controls (fertile and unfertile soil). It promoted the soybean growth such as plant height was 58,8 cm; plant fresh weight was 3.5 g; plant dry weight was 1.55; pod fresh weight was 1.23; pod dry weight was 1.03; the amount of the pod was 3 and the number of the seed was 7.   Keywords : Phosphate solubilizing bacteria, IAA producing bacteria, biofertilizer, soybean.
Isolation and Potential Testing of North Sumatera Berastagi Agricultural Soil In Degrading Marshal Insecticide With Carbosulfan Active Material Nunuk Priyani
International Journal of Ecophysiology Vol. 2 No. 02 (2020): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijoep.v2i02.4683

Abstract

The isolation of bacteria from Berastagi agricultural soil North Sumatera has been done. The aim is to evaluate their ability in degrading carbosulfan. Sixteen bacterial isolates were obtained using selective media Bushnel Hass Agar (BHA) containing 12 ppm of carbosulfan. The parameters observed were the growth of isolates, biosurfactant activity, biosurfactant concentration, and the residue of carbosulfan after 21 days of incubation. The result showed that all isolates were able to degrade carbosulfan as the sole carbon source. Two isolates namely JBM 3 (isolate from citrus agricultural soil Berastagi) and KBM 1 (isolate from cabbage agricultural soil Berastagi) were selected for further test to determine their ability to degrade carbosulfan. The results showed that both of the isolates were able to degrade carbosulfan. Compare to control, isolate JBM 3 was able to decrease the concentration of carbosulfan by 33.33%, while isolate KBM 1 was able to reduce carbosulfan concentration up to 40.47%.
Co-Authors Dessy Merry Silitonga Diah Sri Utami Erman Munir Grace Emalia Masniari Lumbantoruan Isnaini Nurwahyuni It Jamilah Sisca Teresia