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All Journal Jurnal Biotek Medisiana Indonesia The Indonesian Biomedical Journal Universa Medicina Acta Biochimica Indonesiana Bioscientia Medicina : Journal of Biomedicine and Translational Research Majalah Kesehatan Pharmamedika Bioscientia Medicina : Journal of Biomedicine and Translational Research
Ani Retno Prijanti
Department Of Biochemistry And Molecular Biology, Faculty Of Medicine, Universitas Indonesia, Jakarta
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Peran Sitoglobin dalam Mencegah Stres Oksidatif Werdhasari, Asri; Prijanti, Ani Retno; Jusman, Sri Widia
Jurnal Biotek Medisiana Indonesia Vol 5, No 1 (2016)
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i1.5147.

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Efek Hipoxia Mimetic Cobalt Chloride (CoC12 )terhadap Ekspresi mRNA dan Aktivitas Spesifik Manganese Superoksida Dismutase (Mn SOD ) Ginjal Tikus Sandra, Yurika; Retno Prijanti, Ani; Inawati Wanandi, Septelia
Majalah Kesehatan Pharmamedika Vol 9, No 2 (2017): DESEMBER 2017
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/mkp.v9i2.676

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MnSOD is a primary antioxidant that protects cells from oxidative stress due to H2O2 inmitochondrial membranes. This study aims to determine the effect of CoCl2 as hypoxic mimetic agent on mRNA expression of MnSOD and MnSOD-specific activity in rat kidney. Male Sprague dawley rat are induced with 30mg/Kg body weight CoCl2 intraperitoneally. After that, the experiment was divided into 3 groups: 2, 8, and 24 hours incubation after injection. All groups are compared with control group without injection. All of rat were terminated and the kidneys were removed. mRNA expression and specific activity of the kidney MnSOD are measured. After 2 hours, mRNA expression increased up to 42 times, after 8 and 24 hours return to normal. Specific activity of MnSOD in 2 hours after injection has not changed yet, and after 8 and 24 hours increased 1.5 times. This study shows that induction of Hipoxic mimetic CoCl2 enhances mRNA expression and MnSOD-specific activity.
Cullin 1 is not associated with late-onset preeclampsia Samara, Tjam Diana; Liem, Isabella Kurnia; Prijanti, Ani Retno; Andrijono, Andrijono
Universa Medicina Vol 38, No 1 (2019)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (653.458 KB) | DOI: 10.18051/UnivMed.2019.v38.4-9

Abstract

BackgroundLate-onset preeclampsia (PE) is preeclampsia occurring after 34 weeks of gestational age or later.  Cullin 1 (CUL1), a proangiogenic protein, is expressed in the placenta, where an imbalance between proangiogenic and antiangiogenic proteins during gestation can cause disturbance of trophoblast invasion. This defect results in vascular ischemia that may produce preeclampsia. The objective of this study was to determine the correlation between CUL1 as proangiogenic factor and late-onset preeclampsia. MethodsThis study was of analytical observational cross-sectional design and involved 44 preeclampsia patients with ³34 weeks of gestational age (late-onset PE). The CUL1 level in the subjects’ sera, taken before they gave birth, and in homogenates of their placenta, obtained per vaginam or by cesarean section, were examined by the ELISA technique.  Statistical analysis was performed with the Spearman correlation test with significant p value of <0.05.ResultsMedian maternal age was 31 years and median gestational age was 37 weeks.  Median serum CUL1 was 41.78 pg/mL and median placental homogenate CUL1 was 32.24 pg per milligram of total placental tissue protein. There was no significant correlation between serum CUL1 level and late-onset preeclampsia (r=-0.281; p=0.065). There was also no significant correlation between placental CUL1 level and late-onset preeclampsia (r=-0.166; p=0.281).ConclusionSerum CUL1 and placental CUL1 were not correlated with late-onset preeclampsia. However, this study indicated that low serum CUL1 tends to prolong gestational age in preeclampsia.
Comparison of matrix metalloproteinase-9 and E-cadherin expression in early- and late-onset preeclampsia Samara, Tjam Diana; Wibowo, Heri; Liem, Isabella Kurnia; Prijanti, Ani Retno; Andrijono, Andrijono
Universa Medicina Vol. 40 No. 3 (2021)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2021.v40.200-206

Abstract

BACKGROUNDPreeclampsia (PE) is one of the most common pregnancy complications worldwide. Turnover of villous trophoblast is affected by impaired placental perfusion in preeclampsia. Among the various factors that influence pro and antiangiogenic factors in trophoblast invasion of PE are E-cadherin and matrix metalloproteinase-9 (MMP-9). The current classification scheme differentiates PE into two variants early-onset (EO) and late-onset (LO) PE. The aim of this study was to compare MMP-9 and E-cadherin expression between early- (EO) and late-onset (LO) PE. METHODSThis study used a cross-sectional design involving 26 women with gestational age <34 weeks (EO) and 38 women with gestational age ≥34 weeks (LO) from PE patients. Placentas born to preeclamptic mothers were taken in the form of small pieces of the maternal side to measure the levels of MMP-9 and E-cadherin by the ELISA method. Statistical analysis was assessed using the Mann Whitney and independent t-test with a significant p value <0.05. RESULTSSemiquantitative proteinuria levels were significantly higher in EO-PE group compared to LO-PE group (p=0.000). Mean E-cadherin levels were significant lower in the EO-PE group (125.94 ± 54.22 pg/mg) compared to LO-PE group (157.95 ± 54.12 pg/mg) (p=0.024). However, there was no significance difference in median MMP-9 levels between EO-PE group and LO-PE group (p=0.376). CONCLUSIONThis study demonstrate that E-cadherin had lower levels in preeclampsia patients who gave birth <34 weeks. This study indicated that lower levels of e-cadherin can lead to early delivery in preeclampsia patients.
Survivin and Telomerase as Radiotherapeutic Response Predictors of Subjects with Stage IIIB Cervical Squamous Cell Carcinoma Fitriyadi Kusuma; Andrijono Andrijono; Ani Retno Prijanti; Laila Nuranna; Sri Mutya Sekarutami; Bambang Sutrisna; Ferry Sandra
The Indonesian Biomedical Journal Vol 12, No 1 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i1.813

Abstract

BACKGROUND: Cervical cancer is one of the most prevalent cancers in women. Even with similar clinicopathologic features, radiotherapy outcomes are still vary among patients. This research was conducted to measure radiotherapy responses on cervical cancer patients by using Survivin, Telomerase and Cytochrome C.METHODS: Subjects who matched the criteria were selected and requested to fill questionnaires. Subjects were then evaluated with magnetic resonance imaging (MRI) pre- and post-3D conformal radiotherapy. Histopathological study was conducted using resected tumors to determine the differentiation type. Enzyme-linked Immunosorbent Assays for detection of Survivin, Telomerase and Cytochrome C was performed using the resected tumors as well.RESULTS: There were 76 patients in this study. Mean ofage was 50 and diameter of tumor size was 5.35 cm. Mean levels of Survivin, Telomerase and Cytochrome C were 632.82 pg/mL, 5.59 pg/mL and 204.75 ng/mL, respectively. There were significant correlations between radiotherapy response and Survivin (p=0.041) or Telomerase (p=0.022). Subjects with lower Survivin level (<932 pg/mL) had higher 1-year survival rate (63%) than subjects with higher Survivin level (50%). Similar results were obtained for subjects with lower Telomerase level (<5.75 pg/mL), who had higher 1-year survival rate (60%) than subjects with higher Telomerase level (43%).CONCLUSION: Since radiotherapy response is significantly correlated with Survivin and Telomerase levels and subjects with lower Survivin or Telomerase level have higher 1-year survival rate, it can be suggested that Survivin and Telomerase could be potential predictors of radiotherapeutic response for subjects with stage IIIB cervical squamous cell carcinoma.KEYWORDS: cervical cancer, radiotherapy Survivin, Telomerase, Cytochrome C
Syzygium aromaticum (clove) effect on catalase activity due to carbon tetrachloride-induced oxidative stress in rat liver Ani Retno Prijanti; AA Hawali
Acta Biochimica Indonesiana Vol. 1 No. 1 (2018): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v1i1.5

Abstract

Background: Clove is known as antioxidant spice that used in cigarettes, spice for food or soup, and traditional medicine. It is believed that cloves could protect smokers from cigarette-free radicals. Otherwise, the study on clove as an antioxidant was still confused. Objective: To reveal that clove can overcome carbon tetrachloride (CCl4) and its free-radical derives Method: This study was experimental research, using 20 Wistar rats that were divided into 4 groups, Group 1 (CCl4 + cloves 3), group 2 (CCl4 + cloves 1), group 3 (normal control, without being offered treatment), group 4 (positive control, induced by CCl4 and followed by 100 mg alpha-tocopherol), and group 5 (negative control, only induced by CCl4). Rat livers were homogenized and followed with CAT activity measurement using the spectrophotometry method of Mates. Results: There was a significant difference in mean between the groups (p= 0,001). Further test, the Post Hoc showed that there is a significance different between group 1 and 4 (p=0.008), 1 and 5 (p=0.001), 2 and 5 (p=0.001), 3 and 5 (p=0.001), and 4 and 5 (p=0.007). Group 1 (CCl4+Clove3) has the highest catalase activity. Conclusion: Syzygium aromaticum (clove) oral administration with the dose of 200 mg/kg rat body weight against 0.55 mg/kg BW CCl4 show an increase of catalase activity but did not overcome the oxidative stress.
Human serum folate can be measured using folate binding protein linked to enzyme-labeled protein ligand binding assay (ELPLBA) as well as ELISA Muhamad Arif Budiman; Mohamad Sadikin; Ani Retno Prijanti
Acta Biochimica Indonesiana Vol. 1 No. 2 (2018): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v1i2.17

Abstract

Background: Folate is an important substance used for purine and pyrimidine nucleotide synthesis. One measurement of folate that already establishes is using ELISA (Enzyme-linked immunosorbent assay) method. Folate binding protein is a protein that can bind folate, therefore it considered can be used as a tool that can replace antibody dependent ELISA method. Objectives: The aim of this research was to create a method for folate measurement in serum called Enzyme-labeled protein ligand binding assay (ELPLBA) by replacing antibody as used in ELISA method with folate binding protein (FBP) that purified from the whey of milk. Methods: The method is tested using 20 serum samples and compared to ELISA. Folate binding protein was purified from bovine’s milk using ammonium sulfate up to 90% saturated, DEAE-cellulose anion exchange chromatography and affinity chromatography. SDS-PAGE and western blot were used to establish the protein band of FBP that has molecular weight of ~25-35 kDa. ELPLBA was arranged with stationary phase using aminohexyl-agarose, and folic acid linked on it using carbodiimide. Results: The result show there was no significant difference of folate concentration between ELPLBA (14.804 ± 2.795) and ELISA method (13.859 ± 3.638), p = 0.363. Conclusion: ELPLBA method show similarity for determination of folate in serum which was the same as standard folate measurement (ELISA).
Expression of apelin is related to oxidative damage in heart tissue of rats during chronic systemic hypoxia H R Helmi; Frans Ferdinal; Ani Retno Prijanti; Sri Widia A Jusman; Frans D Suyatna
Acta Biochimica Indonesiana Vol. 1 No. 2 (2018): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v1i2.18

Abstract

Background: Chronic systemic hypoxia is severe environmental stress for the heart and might lead to the development of heart failure. Apelin is an endogenous peptide that has been shown to have various beneficial effects on cardiac function. Apelin appears to have a role to play in the ventricular dysfunction and maintaining the performance of the heart. Objectives: In the present study we want to investigate the adaptive response of heart tissue to chronic systemic hypoxia and the correlation with apelin expression and oxidative stress in rat. Methods: An experimental study was performed using 28 Sprague-Dawley male rats, 8 weeks of age. Rats were divided into 7 groups 4 each, namely control group; normoxia (O2 atmosphere) and the treatment group of hypoxia (8% O2) for 6 hours; 1;3;5;7 and 14 days respectively. Body weight and heart weight were measured at each treatment. Ventricular thickness was measured by caliper, Apelin mRNA was measured using real-time qRT-PCR with Livak formula and malondialdehyde (MDA) level was used to assess oxidative stress due to cardiac tissue hypoxia. Results: Macroscopic exams showed hypertrophy at day 7th. The relative expression of Apelin mRNA in hypoxic heart is decreased at the beginning and then increased, starting from day-7 to day-14. The MDA levels were significantly increased from day-7 and were strongly correlated with relative expression Apelin. Conclusion: It is concluded that the increase of Apelin expression is related to oxidative stress in heart tissue of rats during chronic systemic hypoxia.
Alanine amino transferase (ALT) specific activities in long term systemic hypoxic rat brain tissues Rizka Ramadhani; Ani Retno Prijanti
Acta Biochimica Indonesiana Vol. 2 No. 2 (2019): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v2i2.43

Abstract

Background: Brain as a very aerobic organ is sensitive to hypoxia. Energy scarcities must be overcome by gluconeogenesis, which uses alanine or lactate as starting material. The reaction is catalyzed by alanine amino transaminase (ALAT or ALT), also known as glutamate pyruvate transaminase (GPT). Objective: To investigate whether the specific activities of alanine aminotransferase (ALT) increased in hypoxic rat brain. Methods: This experimental study used rats exposed to systemic normobaric hypoxia during 14 days. A group of 5 rats was sacrificed in days 1, 3, 7 and 14. The specific activities of ALT were analyzed in their brains using a reaction coupled with lactate dehydrogenase (LDH) activities. Results: The ALT specific activities in rat brain were very low. There was no significant increase of specific activities during long term hypoxia (p > 0.05). Conclusion: The rat brain ALT has no role in gluconeogenesis.
Cytoglobin expression in rat kidney during exposure to systemic chronic hypoxia Ika Superti Daruningrum; Ani Retno Prijanti; Ninik Mudjihartini; Mohamad Sadikin; Sri Widia A Jusman
Acta Biochimica Indonesiana Vol. 3 No. 1 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i1.55

Abstract

Background: The kidneys in physiological conditions are always in a state of relative hypoxia. Cytoglobin (Cygb) is the newest globin protein found of the globin family. One of the functions of Cygb is in oxygen supply. Cygb expression is found to increase in hypoxic conditions, which are thought to be an adaptation response to hypoxia. Objective: This study aimed to analyze the expression of Cygb in rat kidneys which were exposed to chronic systemic hypoxia. Methods: Twenty five male Sprague-Dawley rats, weighing 150-200 g were used in this experiment. Rats were divided into 5 groups: The control group was exposed to normoxia; the hypoxia groups (10% oxygen / 90% nitrogen) for 1 day; 3 days; 7 days and 14 days. After treatment, rats were sacrificed and their kidneys were taken. Cygb mRNA expression was measured by qRT-PCR, while Cygb protein expression was measured by the ELISA method. Results: The expressions of Cygb mRNA and protein were found to be highest on day 3 of hypoxia and was correlated very strongly and significantly (r2 = 0.96; p <0.05). Conclusion: The highest expression of Cygb on day 3 of chronic systemic hypoxia exposure is suggested as an attempt to restore oxygen supply to the kidneys.
Co-Authors AA Hawali Andriani Andriani Andrijono Andrijono Andrijono Andrijono Bambang Sutrisna Ferry Sandra Fitriyadi Kusuma Frans D Suyatna Frans Ferdinal H R Helmi Ika Superti Daruningrum Inawati Wanandi, Septelia Jusman, Sri Widia Azraki Laila Nuranna Liem, Isabella Kurnia Liem, Isabella Kurnia Luh Putu Ratna Sundari Mohamad Sadikin Mohamad Sadikin Muhamad Arif Budiman Ninik Mudjihartini Riyadh Firdaus Rizka Ramadhani Samara, Tjam Diana Sri Mutya Sekarutami Sri Widia A Jusman Werdhasari, Asri Yurika Sandra