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HASIL PEMERIKSAAN KOLESTEROL TOTAL PADA SERUM SEGERA DIPERIKSA DAN DITUNDA 7 HARI PADA SUHU 2 - 8ºC Karneli Karneli; Alfina Amelda; Asrori Asrori
Klinikal Sains : Jurnal Analis Kesehatan Vol 8 No 2 (2020): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v8i2.1405

Abstract

Background: Lipid profile examination that is often done in a laboratory is an examination of total cholesterol, which can be done by using a serum specimen. Although it is highly recommended to do a direct serum check, there are a number of reasons for delaying the examination, such as a broken device, or a power outage that makes the examination must be postponed. Serum that is not immediately checked can be stored. Storage of specimens is one of the pre-analytic stages, which has a significant risk of error in a laboratory examination. Objectives: This study aims to determine the comparison of the results of total cholesterol in serum which is immediately checked and postponed for 7 days at a temperature of 2-8ºC. Methods: This research was an experimental study with one group pre and posttest design. The population of this study were all Level 1 and 2 Students of Health Analyst Department of Health Ministry of Health, Palembang in 2020, totaling 150 people. The sample of this study was serum from 30 respondents taken by simple random sampling. Total cholesterol levels were checked using the Biosystem bts-350. Results: From the Shapiro Wilk data normality test obtained data with normal distribution. Data analysis continued with t dependent test, p = 0.403 was obtained, it means that p> α (0.05), which means there was no difference in the results of the examination of total cholesterol levels in serum that was immediately examined and stored for 7 days at a temperature of 2-8ºC. Conclusion: It can be concluded that there is no significant difference in the results of the examination of total cholesterol levels in serum that are immediately examined and stored for 7 days at a temperature of 2-8ºC.
ANALISIS KADAR GLUKOSA DARAH DENGAN TINGKAT STRES MAHASISWA TINGKAT 3 PROGRAM STUDI DIII TEKNOLOGI LABORATORIUM MEDIS POLTEKKES KEMENKES PALEMBANG Adella Caesaria; Abdul - Mutholib; Karneli Karneli; Erwin Edyansyah; Nurhayati Nurhayati
Journal of Medical Laboratory and Science Vol 1 No 2 (2021): JMLS : Journal of Medical Laboratory and Science
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.376 KB) | DOI: 10.36086/medlabscience.v1i2.1090

Abstract

ABSTRAK Latar Belakang: Stres merupakan respon tidak spesifik tubuh karena adanya tuntutan yang melebihi kemampuan individu tersebut untuk memenuhinya. Saat stres, tubuh akan merespon dengan menstimulasi area hipotalamus untuk menghasilkan hormon epinefrin yang berfungsi untuk mengubah glikogen menjadi glukosa dan norepinefrin yang berfungsi untuk mencegah penurunan kadar glukosa darah. Kondisi stres yang terus berlangsung dapat mengakibatkan kadar glukosa meningkat sehingga dapat menimbulkan risiko hipertensi dan diabetes melitus. Tujuan penelitian ini adalah untuk mengetahui hubungan kadar glukosa darah dengan tingkat stres mahasiswa tingkat 3 Program Studi DIII Teknologi Laboratorium Medis (TLM) Poltekkes Kemenkes Palembang tahun 2021. Metode: penelitian ini bersifat analitik observasional dengan pendekatan cross sectional. Penelitian dilaksanakan pada tanggal 9 Februari 2021 di Kampus Jurusan TLM Poltekkes Kemenkes Palembang. Sampel penelitian berjumlah 40 orang yang dipilih berdasarkan kriteria inklusi dan eksklusi. Pemeriksaan kadar glukosa darah sewaktu menggunakan metode GOD-PAP dan pengukuran tingkat stres menggunakan kuesioner DASS-42. Hasil: rata-rata kadar glukosa darah dengan tingkat stres normal adalah 83 mg/dL, rata-rata kadar glukosa darah dengan tingkat stres ringan adalah 82 mg/dL, rata-rata kadar glukosa darah dengan tingkat stres sedang adalah 83 mg/dL, dan rata-rata kadar glukosa darah dengan tingkat stres berat adalah 81 mg/dL. Dari hasil uji Anova didapatkan nilai p-value = 0.98 atau > 0,05. Ini berarti bahwa tidak ada hubungan antara kadar glukosa dengan tingkat stres mahasiswa. Kesimpulan: tidak ada hubungan yang signifikan antara kadar glukosa darah dengan tingkat stres mahasiswa. Kata kunci : Glukosa darah, tingkat stres, mahasiswa ABSTRACT Background: Stress is a non-specific response of the body due to demands that exceed the individual's ability to fulfill them. When people get stressed, the body will respond by stimulating the hypothalamic area to produce the epinephrine which functions to convert glycogen into glucose and norepinephrine which functions to prevent a decrease in blood glucose levels. Continuous stress can cause glucose levels to increase so that it can increase the risk of hypertension and diabetes mellitus. The purpose of this study was to determine the relationship between blood glucose levels and stress levels on the third year students of the Medical Laboratory Technology (MLT), a three-year diploma program, Poltekkes Kemenkes Palembang in 2021. Methods: This research is an observational-analytic study with a cross-sectional approach. The research was carried out on February 9, 2021 at the MLT Campus, Palembang. The number of research sample was 40 students who were selected based on inclusion and exclusion criteria. The determination of blood glucose levels used the GOD-PAP method and the measurement of stress levels used the DASS-42 questionnaire. Results: the average blood glucose level with stress level of normal, mild, moderate, and severe were 83 mg/dL, 82 mg/dL, 83 mg/dL, and 81 mg/dL, respectively. From the results of the ANOVA test, the p-value = 0.98 or > 0.05. This means that there is no relationship between glucose levels and student stress levels. Conclusion: there is no significant relationship between blood glucose levels and stress levels of students. Keywords: Blood glucose, stress level, students
FREKUENSI PROTEINURIA PADA PENDERITA LUPUS ERITEMATOSUS SISTEMIK (LES) Assyifa Khoerrunisah; Asrori Asrori; Karneli Karneli; Erwin Edyansyah
Journal of Medical Laboratory and Science Vol 1 No 2 (2021): JMLS : Journal of Medical Laboratory and Science
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.442 KB) | DOI: 10.36086/medlabscience.v1i2.1092

Abstract

ABSTRAK Latar Belakang Lupus Eritematosus Sistemik (LES) merupakan penyakit autoimun multisistem yang mengakibatkan kerusakan organ,jaringan dan sel mediasi karena kompleks imun dan autoantibodi yang berikatan dengan antigen jaringan. LES dapat menyerang satu atau lebih organ, salah satu organ yang banyak diserang adalah ginjal yang dapat menyebabkan komplikasi LES yakni Lupus Nefritis yang memiliki gejala proteinuria. Proteinuria adalah keadaan abnormal dimana jumlah protein dalam urin lebih dari 300 mg dalam urin 24 jam dan 30 mg/dL dalam urin sewaktu. Tujuan penelitian: ini adalah untuk mengetahui frekuensi proteinuria pada penderita Lupus Eritematosus Sistemik (LES) di Palembang tahun 2020, berdasarkan umur, jenis kelamin dan lama sakit. Metode Penelitian: metode penelitian ini adalah deskriptif dengan rancangan cross-sectional. Sampel pemeriksaan yang digunakan adalah urin sewaktu. Jumlah sampel penelitian adalah 29 orang pasien LES di Komunitas PLSS Palembang yang ditentukan dengan teknik purposive sampling. Metode pemeriksaan urin menggunakan metode carik celup (dipstick). Hasil penelitian: menunjukkan sebanyak 6 orang (20.7%) positif proteinuria dan 23 orang (79.3%) negatif proteinuria, Dari 6 orang dengan proteinuria positif, berdasarkan umur ada 6 orang (25.0%) dengan umur berisiko (<50 tahun) dan 0 orang (0.0%) dengan umur tidak berisiko (>50 tahun); berdasarkan jenis kelamin ada 1 orang (50.0%) berjenis kelamin laki-laki dan 5 orang (18.5%) berjenis kelamin perempuan; berdasarkan lama sakit, terdapat 4 orang (57.1%) menderita LES <5 tahun,2 orang (9.1%) menderita LES >5 tahun. Dengan demikian disarankan bagi pasien LES untuk menambahkan pemeriksan fungsi ginjal atau urinalisa pada saat melakukan kontrol rutin. Kata Kunci : Proteinuria, Lupus Eritematosus Sistemik, Autoimun ABSTRACT Background: Systemic Lupus Erythematosus (SLE) is a multisystem autoimmune disease that causes organ, tissue and cell damage due to immune complexes and autoantibodies that bind to tissue antigens. SLE can attack one or more organs, one of the organs that is mostly attacked is the kidney which can cause SLE complications, namely Lupus Nephritis which has symptoms of proteinuria. Proteinuria is an abnormal condition in which the amount of protein in the urine is more than 300 mg in the 24-hour urine and 30 mg/dL in the urine at any time. The purpose of this study: was to determine the frequency of proteinuria in patients with Systemic Lupus Erythematosus (SLE) in Palembang in 2020, based on age, sex and length of illness. Research Methods: This research method is descriptive with a cross-sectional design. The examination sample used was urine at the time. The number of research samples was 29 SLE patients in the Palembang PLSS Community determined by purposive sampling technique. Urine examination method using the dipstick method. The results: showed that 6 people (20.7%) were positive for proteinuria and 23 people (79.3%) were negative for proteinuria. Of the 6 people with positive proteinuria, there were 6 people (25.0%) with age at risk (<50 years) and 0 people. (0.0%) with no risk age (>50 years); based on gender there were 1 person (50.0%) male and 5 (18.5%) female; based on the length of illness, there were 4 people (57.1%) suffering from SLE <5 years, 2 people (9.1%) suffering from SLE >5 years. Thus, it is recommended for SLE patients to add kidney function tests or urinalysis during routine check-ups. Keywords: Proteinuria, Systemic Lupus Erythematosus, Autoimmune
GAMBARAN KEBERADAAN Escherichia coli PADA PRODUK OLAHAN DAGING BAKSO YANG DIJUAL DI KECAMATAN ILIR BARAT I KOTA PALEMBANG TAHUN 2018 Inna Agustin; Refai Refai; Herry Hermansyah; Karneli Karneli
Journal of Medical Laboratory and Science Vol 1 No 2 (2021): JMLS : Journal of Medical Laboratory and Science
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.615 KB) | DOI: 10.36086/medlabscience.v1i2.1097

Abstract

ABSTRAK Latar Belakang : Bakso adalah produk olahan daging berbentuk bulatan atau lain yang dibuat dari campuran daging dan pati. Bakso merupakan produk yang sangat digemari masyarakat selain karena rasanya yang lezat bakso juga merupakan jajanan yang sangat mudah ditemui di mana-mana. Lingkungan, penyimpanan bahan makanan dan kebersihan diri yang kurang higienis dapat menyebabkan bakso tercemar bakteri Escherichia coli. Tujuan penelitian ini adalah untuk mengetahui gambaran keberadaan Escherichia coli pada produk olahan daging bakso yang dijual di Kecamatan Ilir Barat I Kota Palembang Tahun 2018. Metode : Penelitian ini bersifat deskriptif. Teknik sampling yang digunakan adalah purposive dengan sampel bakso yang diambil dari masing-masing 10 pedagang bakso di Kecamatan Ilir Barat I dan tiap sampel diambil sebanyak 250-300gram. Metode pemeriksaan yaitu isolasi dan identifikasi. Hasil : Dari hasil 10 sampel bakso, didapatkan 1 sampel (10%) ditemukan positif Escherichia coli. Berdasarkan lingkungan tempat berjualan yang kurang baik didapatkan hasil dari 2 sampel, 1 sampel (50%) ditemukan positif Escherichia coli. Berdasarkan penyimpanan bahan makanan yang terbuka didapatkan hasil dari 7 sampel, 1 sampel (14,3%) ditemukan positif Escherichia coli. Berdasarkan kebersihan diri yang kurang baik didapatkan hasil dari 4 sampel, 1 sampel (25%) ditemukan positif Escherichia coli. Kesimpulan : Produk olahan daging bakso yang dijual terdapat adanya Escherichia coli. Disarankan para penjual untuk menjaga kebersihan lingkungan dan kebersihan diri serta menyimpan bahan makanan secara tertutup. Bagi konsumen sebaiknya memperhatikan kebersihan makanan yang akan dikonsumsi. Kata Kunci: Bakso, Escherichia coli, Produk olahan daging ABSTRACT Background : Meatballs are processed meat products in the form of circles or other made from a mixture of meat and starch. Meatballs are a product that is very popular with the public, apart from the delicious taste, meatballs are also snacks that are very easy to find everywhere. The environment, food storage and unhygienic personal hygiene can cause meatballs to be contaminated with Escherichia coli bacteria. The purpose of this study was to describe the presence of Escherichia coli in processed meatball products sold in Ilir Barat I District, Palembang City in 2018. Methods: This study is descriptive. The sampling technique used was purposive with samples of meatballs taken from each of 10 meatball traders in Ilir Barat I District and each sample was taken as much as 250-300 grams. The inspection method is isolation and identification. Results: From the results of 10 samples of meatballs, 1 sample (10%) was found to be positive for Escherichia coli. Based on the unfavorable selling environment, the results obtained from 2 samples, 1 sample (50%) was found to be positive for Escherichia coli. Based on the open storage of food ingredients, 7 samples were found, 1 sample (14.3%) was found to be positive for Escherichia coli. Based on poor personal hygiene, 4 samples were found, 1 sample (25%) was found to be positive for Escherichia coli. Conclusion: Processed meatball products sold contain Escherichia coli. Sellers are advised to keep the environment clean and personal hygiene and keep food ingredients closed. Consumers should pay attention to the cleanliness of the food to be consumed. Keywords: Meatballs, Escherichia coli, Processed meat products
BUAH BIT (Beta vulgaris L.) SEBAGAI ALTERNATIF SAFRANIN PADA PEWARNAAN GRAM Dian Adhe Bianggo NauE; Karneli Karneli; Anton Syailendra; Indriyani Syafitri; Syafira Wulandari; Widiah Julianti
Husada Mahakam Vol 12 No 1 (2022): Mei 2022
Publisher : Poltekkes Kemenkes Kalimantan Timur (URL: http://poltekkes-kaltim.ac.id/)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35963/hmjk.v12i1.285

Abstract

Crystal violet and Safranin are carcinogenic dyes in the Gram stain. Safe alternative materials are needed to minimize the use of carcinogenic properties. Betalain is a pigment of Beetroot (Beta vulgaris L.). The purpose of this study was to determine Beetroot (Beta vulgaris L.) as an alternative of Safranin in bacterial Gram staining. It was an experimental research, where to determine the effectiveness of natural dyes, beetroot was made with concentrations of 75%, 90% and 100% with the addition of HCL. The data in this study using the researcher's observation by looking at the existence of bacterial color in Gram staining with beetroot extract substitute the Safranin. Klebsiella pneumonia was used as the Gram negative bacteria. Neither beetroot juice nor beetroot extract stained the Klebsiella pneumonia. However the Klebsiella pneumoniae stained by 100% concentration of beetroot maceration. It can be concluded that Beetroot is less effective for an alternative of Safranin in bacterial Gram staining.