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All Journal EUGENIA Jurnal Penelitian Tanaman Industri Buletin Palma
Hengky Novarianto
Balai Penelitian Tanaman Palma
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

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KERAGAMAN FENOTIPE DAN GENETIK TIGA VARIETAS KELAPA GENJAH KOPYOR ASAL PATI JAWA TENGAH MASKROMO, ISMAIL; TENDA, ELSJE T.; TULALO, MEITY A.; NOVARIANTO, HENGKY; SUKMA, DEWI; SUKENDAH, SUKENDAH; SUDARSONO, SUDARSONO
853-8212
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAKKelapa Genjah kopyor asal Pati, Jawa Tengah merupakankekayaaan hayati asli Indonesia dengan nilai ekonomi tinggi. Informasikeragaman genetik kelapa kopyor masih terbatas. Data keragamanmorfologi dan genetik diperlukan dalam program pemuliaan kelapakopyor. Penelitian ini mempelajari keragaman tiga varietas kelapa genjahkopyor asal Pati yang telah dilepas berdasarkan karakter morfologi,kuantitas endosperma, dan keragaman alel marka SSR. Penelitiandilakukan di Pati dan di Laboratorium Plant Molecular Biology,Departemen Agronomi dan Hortikultura, IPB. Evaluasi dilakukan terhadaptiga populasi kelapa Genjah kopyor (hijau, coklat, dan kuning) dengan 30tanaman sampel untuk setiap populasi. Rataan data morfologi digunakanuntuk menyusun dendogram. Kuantitas endosperma diamati pada satubuah kelapa kopyor per tanaman yang dievaluasi. Karakteristikendosperma dikelompokkan sesuai kategori yang telah ditetapkan. Untuksetiap populasi, analisis marka dengan lima pasang primer SSR dilakukanpada 10 tanaman sampel. Data yang didapat digunakan untuk menentukankeragaman genetik kelapa Genjah kopyor asal Pati. Hasil pengamatanmenunjukkan keragaman morfologis dan alel SSR antar tanaman dalamvarietasnya (keragaman intra-varietas) rendah. Sebaliknya, keragamanmorfologis dan alel SSR antar varietasnya tinggi. Kuantitas endospermakelapa Genjah kopyor asal Pati bervariasi antara skor 1–6. Keragamangenetik yang rendah dalam varietas dan tinggi antar ketiga varietas (coklat,hijau, dan kuning) memperkuat pelepasan ketiganya sebagai varietas lokal.Selain itu, keragaman genetik antar tanaman dalam varietas yang rendahmendukung penggunaan ketiga varietas lokal sebagai tetua dalam programperakitan varietas kelapa kopyor unggul baru. Tetua yang dipilih dapatdiseleksi intra-varietas berdasarkan persentase buah kopyor per tandandan skor kuantitas endosperma yang tinggi.Kata kunci: Keragaman morfologis, keragaman intra dan antar varietas,kuantitas endospermaABSTRACTKopyor dwarf coconuts are mutants from Pati, Central Java havinghigh economic values. However, morphological and genetic diversities ofthis coconut were still limited. Morphological and genetic diversity dataare needed for breeding program. The research objectives were to evaluateintra and inter-specific diversity based on morphology, endospermquantity, and SSR alleles. Field evaluations were conducted in Pati whilelaboratory activities were at Plant Molecular Biology Laboratory,Department of Agronomy and Horticulture, IPB. Three populations ofkopyor dwarf varieties (brown, green, and yellow) were evaluated. Thirtytrees were sampled for each population. The average of morphologicaldata were used to construct cluster analysis. Endosperm quantity wasscored (0 – 9) based on a single nut sample. Ten palms were analyzedusing five SSR loci for each population and used to determine geneticdiversity of populations. Results of observations indicated intra-varietymorphological and SSR allele variations among kopyor dwarf was low.However, inter-variety variations were high. The endosperm quantityscores among kopyor dwarf coconut ranged from 1–6. The low intra-variety and high inter-variety variations among the three kopyor dwarfcoconut supported their release as different local varieties. Moreover, thelow intra-variety phenotypic and genotypic diversities among kopyorbrown, green, and yellow dwarf coconut support their use as parents fornew and superior kopyor coconut variety development in the future. Forsuch purpose, however, it is necessary to conduct intra-variety selection toidentify desirable parents based on high kopyor fruit percentage per bunchand for high kopyor endosperm quantity.Key words: Morphological diversity, intra and inter variety diversities,quantity, endosperm
STUDI PERAKITAN KELAPA HIBRIDA GSK x DMT BERDASARKAN PENANDA RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA) Runtunuwu, Semuel D.; Novarianto, Hengky; Tampake, Heldering; Lengkong, Edy F.
EUGENIA Vol 14, No 1 (2008)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.14.1.2008.7431

Abstract

ABSTRACT   Runtunuwu, S.D. et al. 2008. Assembling Hybrid Coconut of GSK x DMT Based on RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA) Marker. Eugenia 14 (1) : 134-152.   The aimed of this research was : 1. assembling hybrid coconut GSK x DMT (Genjah Salak x Dalam Mapanget) that seeds growth was relatifly homogeneous based on RAPD (Randomly Amplified Polymorphic DNA) marker and 2. to found the assembling method of hybrid coconut that will produce massive seeds relatifely short time will homogeneous plant. It was 65 individu trees observe for the average of famale flower per bunch. The result was 25 individu of coconut GSK has the average flower production > 40 per bunch was analyze the homogeneous genetic with the RAPD marker. Based on the analyze RAPD that were 25 individu of GSK coconut trees have the same genetic average 88 % and 14 individu among that was 100 % have same genetic. Further more that 14 individu of GSK was crossing with the 3 individu of DMT that have high yield per year its was DMT 1188, 1172 and 781. Based on the evaluation for the color of buds, high of buds, the steam circle, the petiole color and the germination time of hybrid coconut seeds from the crossing of GSK x DMT 1188 produce more than    70 % seeds that have same genetic, also for crossing of GSK x DMT 1172 have 9 combination and have more than 70 % that same genetic, 10 combination from crossing GSK x DMT 781 have more than 80 % same seeds growth. Therefore, using the RAPD marker were successfully produced 28 crossing of the hybrid coconut GSK x DMT that have relatifly homogeneous seeds growth.   Keywords : assembling, hybrid coconut GSK x DMT, RAPD.
SELEKSI DAN HIBRIDISASI LONTAR [SELECTION AND HYBRIDIZATION OF PALMYRA] Tulalo, Meity; Mawardi, Sukmawati; Mahayasa, Nyoman; Wagiman, Fransiskus Xaverius; Novarianto, Hengky
Buletin Palma Vol 21, No 1 (2020): Juni 2020
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bp.v21n1.2020.38-46

Abstract

Palmyra palm (Borassus flaballifer, L) is a specific crop in dry climate dry land. The purpose of this research is to select the male and female parent Palmyra trees, hybridization, germination and nursery of Palmyra hybrids. The research was carried out on Sabu Island, Sabu Raijua Regency, East Nusa Tenggara Province from 2017 to 2018. The research method was carried out by determining the population of lontar, followed by selection of female and male palm trees, experiments on collecting male flowers and pollen processing on male Palmyra trees. In the female palm tree, fruit bunches are selected that have not been receptive, when receptive and hybridization until the fruit is physiologically ripe, the pollination is controlled using a spout. The results of the selection of the parent palm trees obtained 28 selected parent trees female Palmyra and 12 selected parent trees male Palmyra. The average height of female selected parent trees is 6.67 m and male selected parent trees are 5.08 m. The observations found that the diversity of the number of female flowers between 9-35 fruits/bunches The percentage of fruit so this artificial cross is 54.90%. Mature fruits ready-to-harvest are obtained around 5-6 months after pollination. As many as 77.20% of the hybrid Palmyra palm seeds that are germinated grow and are ready to be planted at 7 months after germination. ABSTRAKTanaman lontar (Borassus flaballifer, L)  merupakan tanaman spesifik di lahan kering iklim kering. Tujuan penelitian adalah melakukan seleksi pohon induk lontar jantan dan betina, melakukan hibridisasi, perkecambahan dan pembibitan lontar Hibrida. Penelitian dilaksanakan di pulau Sabu, Kabupaten Sabu Raijua, Provinsi Nusa Tenggara Timur sejak tahun 2017 sampai 2018. Metode penelitian dilakukan dengan menetapkan populasi lontar, seleksi pohon induk lontar betina dan jantan dilanjutkan dengan pengumpulan bunga jantan serta prosesing polen. Pada pohon lontar betina dilakukan seleksi tandan buah yang belum reseptif, saat reseptif dan hibridisasi sampai buah matang fisiologis melalui penyerbukan secara terkontrol menggunakan kerodong. Hasil seleksi pohon induk lontar diperoleh 28 pohon induk terpilih (PIT) lontar betina dan 12 pohon induk terpilih (PIT) lontar jantan. Rata-rata tinggi pohon induk terpilih betina yaitu 6,67 m dan pohon induk terpilih jantan 5,08 m. Hasil pengamatan ditemukan keragaman jumlah bunga betina antara 9-35 buah/tandan. Persentase buah jadi persilangan buatan ini adalah 54,90%. Buah matang siap panen diperoleh sekitar 5-6 bulan sejak polinasi. Sebanyak 77,20% dari benih Lontar hibrida yang dikecambahkan tumbuh dan siap ditanam pada umur 7 bulan setelah dikecambahkan.
Co-Authors Budi Santosa Dewi Sukma Edy F. Lengkong ELSJE T. TENDA, ELSJE T. Heldering Tampake Hosang, Meldy Ismail Maskromo Mahayasa, Nyoman Mawardi, Sukmawati MEITY A. TULALO, MEITY A. MEITY TULALO, MEITY Semuel D. Runtunuwu Sudarsono SUKENDAH SUKENDAH, SUKENDAH Tulalo, Meity Aneke Tulalo, Meity Aneke Wagiman, Fransiskus Xaverius