Jurnal Ilmu Dasar
Jurnal ILMU DASAR (JID) is a national peer-reviewed and open access journal that publishes research papers encompasses all aspects of natural sciences including Mathematics, Physics, Chemistry and Biology. JID publishes 2 issues in 1 volume per year. First published, volume 1 issue 1, in January 2000 and avalaible in electronically since 2012 with ISSN 1411-5735 (Print) and avalaible in electronically since 2012 with ISSN 2442-5613 (online). Jurnal ILMU DASAR is accredited SINTA 3 by the Ministry of Education, Culture, Research, and Technology of the Republic of Indonesia (Kemendibukristek) No. 152/E/KPT/2023 (September 25, 2023), Ministry of Research, Technology and Higher Education of the Republic of Indonesia (RISTEKDIKTI), No. 200/M/KPT/2020 (December. 23, 2020). All accepted manuscripts will be published worldwide JID has been indexed in DOAJ, Dimension, OCLC WorldCat, PKP Index, Crossref, Google Scholar, Base, Garuda, and OneSearch. JID have been collaborated in KOBI-ID (Konsorsium Biologi Indonesia) and HKI (Himpunan Kimia Indonesia) since 2017.
Articles
384 Documents
<![CDATA[A Comparison Study for Agrobacterium-mediated transformation Method in Sugarcane (Saccharum spp L.) ]]>
<![CDATA[Bambang Sugiharto]]>;
<![CDATA[Hilda Safitri]]>
<![CDATA[ Jurnal ILMU DASAR Vol 12 No 2 (2011) ]]>
Publisher : <![CDATA[Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember ]]>
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<![CDATA[In order to compare transient expression of gus gene driven by CaMV 35S or rice ubiquitin RUBQ2 promoter, Agrobacterium-mediated transformation was conducted using embryogenic callus and suspension cultures of sugarcane. Histochemical observation of GUS activity after co-cultivation showed that rice ubiquitin promoter produced high level of clear blue spots both in embryogenic callus and suspension cultures, while the CaMV35S promoter was not detected. Regenerated shoots from the infected materials were found higher in suspension cultures than embryogenic callus. The results showed that the rice ubiquitin promoter as well as suspension cultures are more efficient for the Agrobacterium-mediated transformation. However, PCR analysis found there was no amplification of DNA neither for CaMV35 nor rice ubiquitin in genome DNA of regenerated shoot. Lacking of integration of the DNA into the genome showed that the regenerated shoots were not truly transformants might due to the presence of somaclonal variation that was common phenomenon in the regeneration from callus. To avoid the presence of somaclonal variation the transformation was then conduced using in vitro plant without intervening callus phase. Basal segment of in vitro plants prepared from axillary buds of sugarcane were used as the explants for the transformation. The histochemical observation of GUS activity showed that almost all of the infected materials partially exhibited blue color on the basal region. Infected in vitro plants showed rapidly grow and multiplied in theselection medium. Further investigation of the transformation using in vitro plants and Agrobacterium harboring SoSUT1 DNA construct driven by rice actin promoter resulted in development of antibiotic resistant sugarcane shoots. Interestingly the PCR analysis found an insertion of the antibiotic DNA fragment into the sugarcane genome DNA. This result suggests that in vitro plant is an effective target tissue for Agrobacterium-mediated transformation in sugarcane.]]>
<![CDATA[Characterization of Nata De Coco-Benedict Film by Adsorption as Sensor for Glucose in Urine Sample ]]>
<![CDATA[Dwi Indarti]]>;
<![CDATA[A Asnawati]]>
<![CDATA[ Jurnal ILMU DASAR Vol 12 No 2 (2011) ]]>
Publisher : <![CDATA[Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember ]]>
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<![CDATA[Research on nata de coco utilization as a film to immobilize benedict reagent for glucose analysis has been conducted. Immobilization of benedict by nata de coco film was evaluated by using adsorption method and characterization i.e. physical characterization (morphology) and viability characterization of its sensors activity for glucose. Morphological characterization (SEM) showed that the benedict reagent has been absorbed by nata de coco film despite leaching activity of 14.7%, with optimum concentration of Benedict of 0.2682 M, 40 min dyeing time at maximum of 541 nm. Sensor characterization of cellulose nata-Benedict for glucose showed regression coefficient of 0.9895, the detection limit of 780 ppm with a sensitivity of 0.001 and adsorption reproducibility of 0.2013%. Glucose content measurement on urine sample by using Nata De Coco-Benedict based Film Nelson-Somogyi.]]>
<![CDATA[Presence of Arbuscular Mycorrhizal Fungi in Coastal Forest Based on The Salinity Gradients ]]>
<![CDATA[D Delvian]]>
<![CDATA[ Jurnal ILMU DASAR Vol 11 No 2 (2010) ]]>
Publisher : <![CDATA[Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember ]]>
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<![CDATA[The role of arbuscular mycorrhizal fungi is very important in a coastal area with sandy soil, high salinity level and low available of phosphate. However, the salinity levels also affect the diversity and activities of mycorrhizal fungi. This research was aimed to learn about arbuscular mycorrhizal fungi in coastal forest based on the salinity gradients. The results demonstrated that spore density and percentage of mycorrhizal colonization decreased by increasing the soil salinity. The same effect was also showed on the observed spore types. Glomus was found as the dominant spore in the research area.]]>
<![CDATA[Geothermal Reservoir Analysis of Mount Rajabasa Kalianda Potency Area using Resistivity Method and Geothermometer ]]>
<![CDATA[Nandi Haerudin]]>
<![CDATA[ Jurnal ILMU DASAR Vol 10 No 2 (2009) ]]>
Publisher : <![CDATA[Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember ]]>
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<![CDATA[A research has been done to determine geothermal reservoir boundaries of Mount Rajabasa by using 1D sounding and mapping resistivity method. The mapping acquisition data was done by means of Schlumberger configuration for AB/2 = 150 m, AB/2 = 300 m, AB/2 = 450 m and AB/2 = 600 m, next the data was correlated with ID sounding data at 3–2 line and 3–4 line. Geothermal reservoir was found with depth ≤ 450 m with resistivity value is 35 m. The reservoir has been estimated as sandstone tuff which lay in Lampung formation. From 1D sounding correlated at line 3-2 and 3-4 are indicated existing fluid flowed at 20 m under suface. Geothermal reservoir of Mount Rajabasa has temperature 212.08oC and potency of energy is 12.5 MW/Km2. it classified as medium temperature.]]>
<![CDATA[Performance of Multimode-Fiber Coupler with Different Coupler Parameter for Displacement Sensor ]]>
<![CDATA[Samian Samian]]>
<![CDATA[ Jurnal ILMU DASAR Vol 11 No 2 (2010) ]]>
Publisher : <![CDATA[Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember ]]>
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<![CDATA[Three multimode-fiber couplers with different coupling ratios, excess losses and directivities are used to sense adisplacement. The fiber coupler is handmade from 1 mm diameter plastic optical fiber. The light source is 632.8nm He-Ne Laser. OPT 101 (Burr Brown) detector is used to detect the change in power output due to theobject’s displacement. The opted displacement’s resolution is 5 μm. The experiment shows that multimode-ficecoupler with 0.25 ratio, 1.37 dB excess loss, and 25 dB directivity gives the highest performance, which has 4mm dynamic range, 1 mm linear range, and 55.4 μW/mm sensitivity. Higher performance can be reached ifmultimode-fiber coupler with the highest coupling ratio and the lowest excess loss is opted.]]>
<![CDATA[Visualization of Iris Data Using Principal Component Analysis and Kernel Principal Component Analysis ]]>
<![CDATA[Ismail Djakaria]]>;
<![CDATA[suryo Guritno]]>;
<![CDATA[Sri Haryatmi Kartiko]]>
<![CDATA[ Jurnal ILMU DASAR Vol 11 No 1 (2010) ]]>
Publisher : <![CDATA[Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember ]]>
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<![CDATA[Principal component analysis (PCA) is a method used to reduce dimentionality of the dataset. However, the use of PCA failed to carry out the problem of non-linear and non-separable data. To overcome this problem such data is more appropriate to use PCA method with the kernel function, which is known as the kernel PCA (KPCA). In this paper, Iris dataset visualized with PCA and KPCA, that contains are the length and the width of sepal and petal. ]]>
<![CDATA[Laser Induced Thin Film Production (LITFP) Using Nitrogen (N2) Laser Deposition ]]>
<![CDATA[Syamsir Dewang]]>
<![CDATA[ Jurnal ILMU DASAR Vol 10 No 2 (2009) ]]>
Publisher : <![CDATA[Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember ]]>
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<![CDATA[Laser induced thin film production (LITFP) technique was employed for making Lead (Pb) thin film by nitrogen laser ablation in miniature scale. The energy of nitrogen laser operated at 12.5 kv, 90 torr was 3.5 mJ with 5 ns pulse duration, thus producing peak power at around 0.7 MW. Pb plasma of 1 cm diameter was generated in each laser bombardment, producing thin film above the glass substrate. The thin film characteristics were measured by the thickness and surface morphology using scanning electron microscope (SEM). It was proved that there was a linear relationship between the number of laser shots and film thickness.]]>
<![CDATA[In Vitro Acitivities Test of Papaya Leaf Extract (Carica papaya. LINN) as an Antimalaria Agent ]]>
<![CDATA[Johanis F Rehena]]>
<![CDATA[ Jurnal ILMU DASAR Vol 11 No 1 (2010) ]]>
Publisher : <![CDATA[Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember ]]>
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<![CDATA[Based on WHO’s estimation, there are at least 300-500 million people in the world infected by Malarian disease every year, 110 million people show the symptom, and 2.7 million people died. People who suffer from malaria in Kairatu regency are 1.296 people. Therefore, the invention of Malarian medicines is hoped to provide new medicine with mechanism and potential target and safe for the society. The objective of this study is to find out the role of extracted papaya’s leaf Cibinong and Solo Variety as antimalaria. The method is laboratory experimental, which is the sample of papaya’s leaf about 400 gram taken from Kairatu regency Maluku Province. Parasite used is Plasmodium falciparum strain G2300. antimalaria activity test in vitro is done toward the result of extracted papaya’s leaf dissolved in DMSO then it is done on flat titer with 24 holes. With level of parasitemia 1% and hematokrit 5%, and then is counted the percentage of parasitemia and growth percentage. Antimalaria test result on papaya’s leaf Cibinong variety with probit program show the value of IC50, 2.7821nanog/ml and Solo variety of Papaya is IC 50, 2.14279, in other word, that cibinong veriety and solo in vitro is active as an antimalaria. ]]>
<![CDATA[The Treatment of the Corn-Knob as A Raw Material for Making Furfural ]]>
<![CDATA[Nurul Hidajati]]>
<![CDATA[ Jurnal ILMU DASAR Vol 8 No 1 (2007) ]]>
Publisher : <![CDATA[Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember ]]>
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<![CDATA[This research is conducted to determine the furfural rendement of the corn-knob with the varieties of heating periods of 2; 2.5; 3; 3.5; and 4 hours. This methods used were, first, the hydrolysis of the corn-knob using HCl with the varieties of heating periods as mentioned above. Then it was continued by the extraction and purification of the furfural through distillation. The resulted furfural is identified by the acetic-anilin color reaction, infrared spectrophotometer, refractive index and GC-MS. The identification result was compare with the furfural standard. The result of this research shows that average rendement was 8.43% for the heating period of 2 hours, 9.67%, for 2.5 hours, 11.57% for 3 hours, 13.30% for 3.5 hours and 12.59% for 4 hours.]]>
<![CDATA[Nyale Sea Worm As Antibacterial Substances ]]>
<![CDATA[Dwi Soelistya Dyah Jekti]]>;
<![CDATA[Agus Abhi Purwoko]]>;
<![CDATA[Zainul Muttaqin]]>
<![CDATA[ Jurnal ILMU DASAR Vol 9 No 2 (2008) ]]>
Publisher : <![CDATA[Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember ]]>
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<![CDATA[Nyale is a sea worm that belongs to class of polychaete. It appears on a huge crowd, usually five days after the monsoon in February, at the surface of the sea for breeding. The colors of the female and male worms are green and brown, respectively. The worms are collected in nyale season, freezed-dryer, and extracted with ethyl acetate. Antimicrobial activity properties of the male worm extract are carried out toward benthos bacteria and clinical isolate bacteria using ciprofloxacin as comparing agent. The results show that, after colom chromatography, fraction number 1 and 4 have the best antibacterial activities (broadest spectrum) toward clinical isolate bacteria. All eleven fractions show also antibacterial activities toward nine benthos bacteria. The minimum inhibitory concentration (MIC) of fractions 1 and 4 toward six clinical isolate bacteria is 100 μg/ml. Meanwhile, fraction 4 exhibits two peaks in its HPLC chromatrogram.]]>
