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ANALISIS PERTUMBUHAN PROTEIN SEL TUNGGAL (PST) BAKTERI Bacillus cereus DENGAN MEDIA YANG BERBEDA Nasution, Mutiara Naimi; Feliatra, Feliatra; Effendi, Irwan
Jurnal Perikanan dan Kelautan Vol 26, No 1 (2021)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.47-53

Abstract

The Media is a material consisting of a mixture of nutrients used by microorganisms to grow and breed. Molasses and liquid waste know one of the alternative media which has the least inexpensive carbon source and is easily obtained from the waste food industry that is rich in nutrients and minerals, so it has the potential for bacterial growth of Bacillus cereus. The consetration of molasses used for growth medium is 1%; 1.5%; and 2% while liquid waste knows 8%, 10%, and 12%. Cell growth is measured by the method of Spectrophotometry, total plate count (TPC) and biomass while the incubation period of cell growth is measured every 0, 6, 12, 18, and 24 hours. The results showed from the molasses + skim milk and the tofu liquid waste + skim milk, the best medium of bacterial cell growth of B. cereus is a liquid waste know isolates K 12%. While the best growth concentrations molasses Medium bacterial B.cereus was N2% and K2%.
Karakterisasi Molekuler Bakteri Probiotik Ikan Kerapu Bebek Berbasis Teknik 16S Ribosomal DNA Feliatra, Feliatra; Andrito, Wahyudi
Jurnal Natur Indonesia Vol 10, No 1 (2007)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (95.052 KB) | DOI: 10.31258/jnat.10.1.13-17

Abstract

The aim of research was to find the molecular characteristics of probiotic bacteria in the digestive tract of grouper fish (Chromileptes altivelis) based on 16S ribosomal DNA technique. The bacteria were isolated from stomach and intestine of the fish and were analized in the Marine Microbiology Laboratory of fishery and marine Science Faculty of Riau University. The bacterial DNA were isolated by using a PCR (polymerase Chain Reaction) and was conducted in the biotechnology laboratory of Diponegoro University, Semarang. The purified DNA was sequenced in the BPPTTangerang. Result shown that 6 bacterial species may be potential as probiotic. There were Bacillus velesensis strain CR-11, Vibio alginolyticus A3G-2, Bacillus cereus site2S, uncultured bacterium clone BB3S16S-17, Bacillus subtillus strain CICC10066, and Bacillus flexus strain LF-3. these bacteria grow well at pH 2 and this indicated one of probiotic bacteria characteristics.
GROWTH OF HETEROTROPHIC BACTERIA IN SEA WATER POLLUTED BY SURF DETERGENT Pratama Novian, Dewan; Effendi, Irwan; Feliatra, Feliatra
Asian Journal of Aquatic Sciences Vol 1 No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.136 KB) | DOI: 10.31258/ajoas.1.1.29-34

Abstract

The research was conducted from February 2016 to April 2017, aimed to determine the effect of adding different concentrations of detergent namely SURF on the growth of bacterial populations in the seawater column. This research used experimental method with Completely Randomized Design. Seawater samples were analyzed at Marine Microbiology Laboratory, Fisheries and Marine Faculty of Riau University. Based on the results of the study, from day 0 untill day 10 after given detergent with different concentrations, bacteria experienced a decreasing trend of growth, in day 15 untill day 20, the trend of bacterial growth contaminated by detergent with different concentration relatively increased to stagnant. Based on the result of this study the addition of different detergent concentration in each treatment affect the growth of heterotrophic bacteria population in the sea water, where the higher concentration of detergent added the lower the ability of bacteria to grow and the ability of heterotrophic bacteria to survive at different concentrations of detergent, it can be concluded that the ability of heterotrophic bacteria to grow from days 0 to 20 has decreased in each of its concentrations.
EFFECTIVENESS TEST OF NIPAH EXTRACT AS LARVACIDE ON MOSQUITO LARVAE (Aedes aegpti) Mariode Triputra Purba, Thadeus; effendi, irwan; Feliatra, Feliatra
Asian Journal of Aquatic Sciences Vol 2 No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.522 KB) | DOI: 10.31258/ajoas.2.1.72-78

Abstract

This research was conducted in January - February 2019. The purpose of this study was to examine the effectiveness of mangrove extracts on stems and fruit on the growth of Aedes aegypti mosquito larvae and to know Lethal Concentration LC50 and Lethal Time LT50. The method used is the experimental method, with the experimental design is a completely randomized design (CRD). The treatment of nipah fruit and stem extract concentrations was 3%, 6% and 9% with contact time of 15 minutes, 30 minutes, 45 minutes 60 minutes, 120 minutes, 480 minutes and the number of replications 3 times with 2 trials. To find out (LC50) and (LT50), the data was analyzed using the SPSS program. The sample of this research is stem and nipah fruit taken in the mangrove area of Kayu Ara Village, Siak Regency. Aedes aegypti larvae are taken at a water reservoir next to the Arfaunas Mosque in Tampan District. The results showed that the stem and nipah fruit extracts had effective killing power against Aedes aegpti larvae. The extract that was more effective in killing mosquito larvae was the nipah fruit extract compared to the nipah stem extract seen in the number of larval deaths with LC50 = 3.398% and LT50 = 3.540 hours. This shows that the level of toxicity is quite toxic.
EFFECTIVITY OF NIPAH (Nypa fruticans) EXTRACT ON (Aedes aegypti) LARVAE IN BRACKISH WATER Rady, Rady; Effendi, Irwan; Feliatra, Feliatra
Asian Journal of Aquatic Sciences Vol 2 No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.174 KB) | DOI: 10.31258/ajoas.2.2.107-118

Abstract

The most commonly used method of vektor control is temephos (the synthetic larvicide). Currently in some areas there has been resistance of aedes aegypti larvae to temephos, so that natural larvicide is required as an alternative. Nypa exctract contains flavonoid compounds that are respiratory toxins that can kill aedes aegypty larvae.This research was conducted in January - February 2019. The purpose of this study was to determine the inhibitory power of nipah extract against larvae in brackhish water. The results of this study are expected to increase knowledge and provide information about the pontential of nipah as a soursce of bioactive compounds. The method used is the experimental method, where the location of the research sampel was obtained at one sampling location point in the Sungai Kayu Ara village, Siak Riau province. The results showed that the average mortality of larvae in nipah laeves in the 60th minute was 55, 80, 100 and 100%. While root extracts have larvae deaths as many as 60 minutes, namely 100, 90, 90, and 100%. For the average mortality of larvae at the root of nipah there are 60 minutes, which are 95, 100, 100 and 100%. As wel as positive and negative controls as a comparison. So it can be compared to 3 administration of nipah extract both leaves, roots and midrib so that the three conncentrations used are better than the use of midrib extract compared to leaf and root extract. It can be concluded that the more concentration used the higher the mortality of larvae is the concentration of 12%. For the concentration used the percentage of larvae mortality in the test media is as much as 6%, 9% and 12%. Every death of larvae has reristance to different bodies so that at the 30th minute there is a difference in mortality of larvae wich a given concentration. The larvae species used are aedes aegyti larvae. The highest mortality of dead es was found in midrib extract and also positive controls. So that the death of larvae is very effective against the given solution has the same ability. The mortality of A. aegypti larvae were then analyzed using probit analysis to obtain LC50 (Lethal Concentration 50) and LT50 (Lethal Time 50) values. The results of the probit analysis of leaf, root and midrib extracts are the estimation of LC50 1.493, 2.618, 0.005. while leaf , root and midrib extracts at the LT50 estimate were 2.841, 1.579, 2.227
ADDITION OF SUPERIOR HETEROTROF BACTERIA MIXED IN FEED TO IMPROVE HEALTH OF SALINE TILAPIA Oreochromis niloticu Maraditha Simanjuntak, Lewi; Lukistyowati, Iesje; Feliatra, Feliatra
Asian Journal of Aquatic Sciences Vol 2 No 3 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (930.339 KB) | DOI: 10.31258/ajoas.2.3.170-180

Abstract

This research was conducted in May 2019 until July 2019 which took place at the Laboratory of Marine Microbiology and the Laboratory of Fish Parasites and Diseases at the Faculty of Fisheries and Maritime Affairs, Riau University. The purpose of this study was to determine the effect of the addition of superior heterotrophic bacterial isolates that were sprayed on feed could affect the health of saline tilapia (Oreochromis niloticus) through erythrocyte and leukocyte blood images. The treatments are Kn, Kp, feed sprayed with B.cereus heterotrophic bacterial isolate solution (0.15% dose), feed sprayed with heterotrophic V. fluvialis bacterial isolate solution (0.15% dose), feed sprayed with combined Isolate solution. The results of the study of the addition of superior heterotrophic bacterial isolates sprayed on feed can improve the health of saline tilapia (Oreochromis niloticus) after 30 days of maintenance and post-infection of Aeromonas hydrophila bacteria showed that the treatment of P3 (combined isolates) which is the best treatment with a total leukocyte value of 89.67 x 103 cells / mm3, lymphocytes 80.33%, neutrophils 10.67%, monocytes 9.66%, total erythrocytes 235.00 x 104 cells / mm3, hematocrit 32.00% and hemoglobin 7.33 g / dL and weight growth absolute 7.05 g, a survival rate of 83.33%.
ISOLATION AND ANTAGONISM OF BACTERIA TEST OF BIOTA IN THE MANGROVE ECOSYSTEM KAYU ARA RIVER SIAK REGENCY Moritania, Rizky; Effendi, Irwan; Feliatra, Feliatra
Asian Journal of Aquatic Sciences Vol 2 No 3 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (676.472 KB) | DOI: 10.31258/ajoas.2.3.190-196

Abstract

The General area is the mangrove biodiversity and marine life that have biological activity that likely has the same pharmacological potential as an antimicrobial compound. The purpose of this research is to know the bacterial antagonism in isolation from mangrove, mangrove crabs, snails and fish tembakul against pathogenic bacteria (Aeromonas hydrophila, Aeromonas salmonicida, Edwardsiella ictaluri). This research dimula from May – June 2019. From the results of research that has been done shows that isolates that have the capability of antagonism against pathogenic bacteria test three (A. salmonicida, A. hydrophila and E. ictaluri) were isolate CS7 with category stronger, isolate CS6, CS5, CS4, AI4, AI5, CS9 AI2, with weak category, whereas isolates CS1, B22, B33, B44, AI6 AI7 AI8, was only able to inhibit pathogenic test of 2 with a weak category. The results of the DNA analysis of the 16S rRNA sequence with the use and analysis of BLAST obtained that isolates the Bacillus cereus species are included.
SECONDARY METABOLITE CHARACTERISTIC OF HETEROTROPHIC BACTERIA PRODUCTION AS ANTIMICROBIA AT DIFFERENT SALINITY Apriyola, Nadya; Feliatra, Feliatra; Nurulita, Yuana
Asian Journal of Aquatic Sciences Vol 3 No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.3.2.147-157

Abstract

This research was conducted from March-June 2019. The purpose of this study was to determine the characteristics of secondary metabolites prduced by of heterotrophic bacterial from sea water Sungai Kayu Ara Village, Siak Regency as an antimicrobial and to determine the storage time of these bacteria by measuring at the optimal growth time. Five bacterial secondary metabolite extracts used were B, C, D, and H (B. cereus) and J (V. fluvialis) obtained from the collection of Marine Microbiology Laboratory, Department of Marine Sciences, Faculty of Fisheries and Marine sciences, University of Riau. phytochemical test showed that extracts of isolates B, D, and H contained saponin compounds, while isolate J contained flavonoid compounds, however, all extracts contained alkaloid compounds. Antimicrobial test indicated that J extract inhibited A. Hydrophila at concentration 500 µg/ml but the extract could not inhibit V. algynolyticus and Pseudomonas sp concentrations determined. In the bacterial storage time test, the optimal growth of each bacterial concentration at was 7th day incubation and decreased on the 14th day.
MOLECULAR IDENTIFICATION OF PROTEOLITIC BACTERIA FROM MANGROVE SEDIMENT IN DUMAI MARINE STATION Zebua, Abdi Hikmat Petra; Nursyirwani, Nursyirwani; Feliatra, Feliatra
Asian Journal of Aquatic Sciences Vol 3 No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.3.2.179-188

Abstract

Proteolytic bacteria have an important role in the degradation of complex compounds to a simple compounds by the enzyme proteases. Sediment in the mangrove ecosystem of Dumai Marine Station may contain the bacteria. This research aims to identify the proteolytic bacteria of mangrove sediments molecularly and to examine the antagonism of pathogenic bacteria. The method used in this research was a survey. The results obtained 10 bacterial isolates (AZ1, AZ2, AZ3, AZ4, AZ6, AZ10, AZ11, AZ15, AZ18, and AZ20). Identification using 16S rRNA analysis revealed that 3 isolates showed different results, namely AZ2 had a similarity to Bacillus proteolyticus with strain MCCC 1A00365, AZ6 had a similarity to the bacteria Bacillus monlinensis with strain BL4-6 and AZ20 had similarity to the bacterium Bacillus toyonensis with strain MCCC 1A00365 BCT-7112. Three of the 10 isolates that had the highest inhibition against pathogens. Isolates AZ2 shovued inhibition zone against Pseudomonas aeruginosa with a diameter of 9.65 mm, AZ6 shovued inhibition zone against Escherichia coli bacteria with a diameter of 5.18 mm, and AZ20 shovued inhibition zone against Vibrio alginolyticus bacteria with a diameter of 4, 01 mm.
SENSITIVITY TEST OF VIBRIO sp BACTERIA ISOLATED FROM DUMAI SEA WATERS TO ANTIBIOTICS (CIPROFLOXACIN, ERYTROMYCIN AND STREPTOMYCIN) Fitri, Febi Anisa; Feliatra, Feliatra; Yoswati, Dessy
Asian Journal of Aquatic Sciences Vol 3 No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.3.2.189-192

Abstract

The research was conducted from Feb until April 2019. The purpose of this study was to examine the resistance of Vibrio sp bacteria toward antibiotics (ciprofloxacin, streptomycin and erythromycin) isolated from Dumai sea waters. The spread of Vibrio sp bacteria has been found in sea waters, Vibrio sp bacteria have been found on the surface of faecal contaminated water and the existence of the population is related to organic waste disposal. Antibiotic susceptibility test was performed using Kirby-Bauer method. Resistance test showed that isolated V2, V9, V11, V13, V19, V23, V24 dan V28 were resistant to antibiotic ciprofloxacin, streptomycin and erythromycin with inhibitory zones ranging from 7,1 to 31,7 mm. . Isolated V2 and V9 are resistant to erythromycin, isolated V11, V13, V19, V23 and V28 are resistant to streptomycin and isolated V24 is resistant to ciprofloxacin.