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All Journal Jurnal Akuakultur Indonesia Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Jurnal Ilmu Lingkungan Jurnal Lahan Suboptimal Sains Tanah IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Science and Technology Indonesia Jurnal Penelitian Sains IJEMS (Indonesian Journal of Environmental Management and Sustainability) Molekul: Jurnal Ilmiah Kimia Berkala Penelitian Hayati SRIWIJAYA JOURNAL OF ENVIRONMENT BIOVALENTIA: Biological Research Journal

Hary Widjajanti

Jurusan Biologi FMIPA, Universitas Sriwijaya, Sumatera Selatan, Indonesia

Author-ID : 201755

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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EFFECT OF LIME APPLICATION ON INDIGENOUS NITROGEN-FIXING BACTERIA IN TIDAL SOILS MANAGED FOR THAN 30 YEARS Ridwan, Rahmawati; Budianta, Dedik; Widjajanti, Hary
BIOVALENTIA: Biological Research Journal Vol. 3 No. 2 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

The study was conducted to determine the effect of land typology and lime on the population of indigenous nitrogen-fixing bacteria and soil pH, and to analyze the effect of soil typology interaction and lime on the population of indigenous nitrogen-fixing bacteria and soil pH. Sampling of soil in tidal land of Mulya Sari and Banyu Urip Village of Banyuasin Regency, South Sumatera. Then the sample was studied at the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Inderalaya. During January-May 2017. Based on the results of the study known the soil typology of the total population of nitrogen-fixing bacteria, the highest population of nitrogen inhibitor bacteria in the second week of soil typology D was 6.0x107 and the highest pH of 5.18 in typology B on week Third, the lime treatment that affected the population of indigenous nitrogen-fixing bacteria, obtained the population of bacteria as much as 9.3x105 in the second week and the highest soil pH 4.93 in the second week, while the results of the interaction between soil typology and lime treatment only affect the population of bacterial inhibitor Indigenous nitrogen, with bacterial population of 3.5x107 in soil typology D in the second week, but this interaction did not significantly affect soil pH, with the highest pH measurement of 5.33 on the soil typology D.Keywords: nitrogen-fixing bacteria, tidal land, lime treatment

POTENCY AND ACTIVITY OF SECONDARY METABOLITE OF Trichoderma harzianum AC1(b) J2 INHIBITOR GROWTH Colletotrichum capsici IPBCC 13.1098 Nurkayah, Nurkayah; Nurnawati, Elisa; Widjajanti, Hary
BIOVALENTIA: Biological Research Journal Vol. 5 No. 1 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Trichoderma harzianum is a fungus that can produce secondary metabolites which able to inhibit the growth of Colletotrichum capsici. a pathogenic fungus causing anthracnose in plants. the Aims of this research were to obtain and analyze the ability of secondary metabolites of Trichoderma harzianum AC1 (b) J2 isolated from Scleria poaeformis. to inhibit the growth of Colletotrichum capsici IPBCC13.1098. The secondary metabolite eluat of Trichoderma harzianum were tested for the antifungal activity toward Colletotrichum capsici IPBCC 13.1098. Fractionation was carried out by Column Chromatography and produced 31 eluat. The secondary metabolite eluat of Trichoderma harzianum with the highest inhibition zone diameter was eluat 1 with 8.4 mm in diameter. The secondary metabolite eluat with the highest value of inhibition zone was carried out by MIC test and thin layer chromatography (TLC). Based on the MIC results, the minimum inhibitory concentration of the secondary metabolite of Trichoderma harzianum to fungus Colletotrichum capsici was 250 ppm with inhibition zone diameter was 0.10 mm. The results of TLC showed orange spots on the TLC plate that indicated alkaloite compounds.

EXPLORATION OF ENDOPHYTIC FUNGI OF DRAGON SCALEâ€™S FERN (Pyrrosia piloselloides (L.) M.G. Price) AS AN ANTIBACTERIAL SOURCES Asiandu, Angga Puja; Widjajanti, Hary; Nurnawati, Elisa
BIOVALENTIA: Biological Research Journal Vol. 5 No. 2 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Endophytic fungi are fungi which live inside the host plant tissue and have been undergone a horizontal gene transfer process. Endophytic fungi are able to synthesize the same bioactive compounds which synthesized by their host plants. The host plant used in this research was dragonâ€™s scales fern (Pyrrosia piloselloides (L.) M.G. Price). Dragonâ€™s scales fern produces various of bioactive compounds which used as antibacterial agents such as polyphenols. This research was aimed to obtain endophytic fungi isolates from trophophyll fronds and sporophyll fronds of dragonâ€™s scales fern, to determine the antibacterial activity of the secondary metabolite extracts of endophytic fungi, to determine the Minimum Inhibitory Concentration (MIC), to determine the characteristics of the endophytic fungi isolates which potentially as antibacterial source. Based on the research, 13 endophytic fungi isolates were obtained from dragonâ€™s scales fern fronds consist of 5 isolates from trophophyll fronds and 8 isolates from sporophyll fronds. The antibacterial activity test showed that the extract of secondary metabolites of the isolate DTP2 had the highest inhibition zone diameter against E.coli 14.82 Â± 4.05 mm, DTP4 against S.aureus 8.80 Â± 0.03 mm and DSP4 against S.dysentriae 10.15 Â± 0.36 mm. MIC of ethyl acetate extracts of secondary metabolites of isolate DTP2 against E.coli was 125 Âµg/mL, DTP4 against S.aureus was 125 Âµg/mL and DSP4 against S.dysentriae was 31.25 Âµg/mL. The endophytic fungi isolate DTP2 identified as Aureobasidium melanogenum, DTP4 identified as Penicillium alliisativi and DSP4 identified as Aspergillus flocculosus.

ANTIOXIDANT ACTIVITY of THE SECONDARY METABOLITES PRODUCED by ENDOPHYTIC FUNGI ISOLATED from JERUJU (Acanthus ilicifolius L.) PLANT Putri, Amanda Rahmaniah; Salni, Salni; Widjajanti, Harry
BIOVALENTIA: Biological Research Journal Vol. 5 No. 2 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Twelve endophytic fungi were isolated from Jeruju plant. Three of which have the best antioxidant activity, namely Culvularia lunata DJS1, Diaporthe phaseolorum DJS2, and Colletotrichum siamense BJS4. The fungi can produce secondary metabolites that have the potential as antioxidant compounds. This study aims to isolate the antioxidant compound using a chromatography column and test the antioxidant activity using DPPH method. Isolation of secondary metabolites produced 40 eluates. The IC50 value of DJS111 and BJS42 eluates were 10.20 Âµg/ml and 20.89 Âµg/ml, which is an antioxidant compound with very strong activity. The IC50 value of DJS24 eluate was 54.45 Âµg/ml, which is strong antioxidant activity. DJS111 eluate has antioxidant activity that approaches to ascorbic acid as a standart antioxidant compound (7.76Âµg/ml). From TLC, DJS111 contains flavonoid compound and DJS24 and BJS42 contain terpenoid compound.

The Usage of Garlic Extract (Allium sativum) to Cure Pangasius Fish (Pangasius hypophthalmus) Infected by Aeromonas hydrophylla Muslim Muslim; Hotly Maraulina; Harry Widjajanti
Jurnal Akuakultur Indonesia Vol. 8 No. 1 (2009): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The purpose of this research was to know effectivity of the garlic extract to cure Pangasius catfish that infected by A. hidrophylla bacteria. This research was done on February to April 2007, at Aquaculture Laboratory FP UNSRI. This research used Completely Randomised Design (CRD) with five treatments and three replications. The concentration of the garlic extract that used were 0 % (control), 0.2 %, 0.4 %, 0.6 %, 0.8 %. The parameters inspected include SR of fish that infected; SR of fish had been treatment, Relative Percent Survival (RPS), clinical symptom and water quality. The highest survival rate percentage is treatments A4 (0.8 %) with average value 66.66 %. The best concentration of garlic extract that can heal the fish until ≥ 50 % (RPS value) from totally sample of fish were treatments A4 (0.8 %), A3 (0.6 %), and A2 (0.4 %). The clinical symptom after cure and care as long as fourteen days indicated in control (without garlic extract has been found hard damage bodies organ and the mortality fish effect, but in treatments A1 (0.2 %), A2 (0.4 %), A3 (0.6 %), and A4 (0.8 %) recover after submerged with garlic extract. The water quality parameters during experiment in each treatments such as temperature was 27 - 29 oC, pH 6-6.5, Dissolved Oxygen 5.24 - 6.87 mg/l, and Ammonia 0.09 - 0.46 mg/l.Keywords: garlic extract, pangasius fish, A. hydrophylla bacteria ABSTRAKTujuan penelitian ini untuk mengetahui efektivitas ekstrak bawang putih untuk mengobati penyakit bakterial pada ikan patin yang disebabkan A. hidrophylla. Penelitian ini dilaksanakan pada bulan Februari - April 2007, di Laboratorium Budidaya Perairan, FP UNSRI. Penelitian ini menggunakan rancangan acak lengkap, 5 perlakuan dan 3 ulangan. Konsentrasi ekstrak bawang putih yang digunakan adalah 0 % (kontrol), 0,2 %, 0,4 %, 0,6 %, 0,8 %. Parameter yang diamati meliputi SR ikan yang terinfeksi, SR ikan setelah diberi perlakuan, RPS, gejala klinis serta kualitas air. Persentase SR tertinggi pada perlakuan A4 (0,8 %) sebesar 66,66 %. Konsentrasi ekstrak bawang putih yang dapat menyembuhkan ikan sampai ≥ 50 % (nilai RPS) dari jumlah total ikan berturut-turut adalah perlakuan A4 (0,8 %), A3 (0,6 %), dan A2 (0,4 %). Gejala klinis setelah pengobatan dan pemeliharaan selama 14 hari menunjukkan pada perlakuan kontrol (tanpa ekstrak bawang putih) terdapat kerusakan organ tubuh paling parah dan menyebabkan ikan mati, sedangkan pada perlakuan A1 (0,2 %), A2 (0,4 %), A3 ( 0,6 %), dan A4 (0,8 %), gejala klinis pada tubuh ikan berangsur sembuh setelah direndam dengan ekstrak bawang putih. Parameter kualitas air selama pemeliharaan pada masing-masing perlakuan yaitu suhu (27-29oC), pH (6-6,5), O2 terlarut (5,24-6,87 mg/l) dan amonia (0,09-0,46 mg/l).Kata kunci : ekstrak bawang putih, ikan patin, bakteri A . hydrophylla.

Eukaryote microbes potential for bioflocs in the swamp aquaculture Marini Wijayanti; Tanbiyaskur; Dade Jubaedah; Ade Bayu Saputra; Karta Sari Genti; Agustina; Nabila Saraswati; Siti Yuliani; Hary Widjajanti
Jurnal Akuakultur Indonesia Vol. 19 No. 1 (2020): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Eucaryote microbes have an important role in forming bioflocs in the brackishwater aquaculture ponds. Bioflocs become potential live feed for milkfish or crustacea. This study aimed to identify the potential of eukaryotic microbes in the brackishwater aquaculture as the biofloc candidates potential development. This study was done through the water quality assessment and potential indigenous microbes isolation approach. Sampling was retrieved from the water subcomposite and sediment on each intertidal inlet and outlet. The water quality of brackishwater pond and intertidal swamp tended to lack of nutrient as containing inoptimal dissolved oxygen, salinity, pH, and ammonia content for the milkfish culture. Therefore, liming and fertilizing should be done to enrich the indigenous microbes. Eucaryote microbe isolated from the enrichment media was the aquatic fungi (multicellular and unicellular fungi) and microalgae (bacillariophyta, chlorophyta, and cyanophyta). Fungi and yeast formed a floculation with microalgae. This form will become a biofloc candidate as a live feed and water quality controller for the development of brackishwater aquaculture in the swamp area. Keywords: eucaryote microbes, biofloc, aquaculture, pond, brackish water swamp ABSTRAK Mikrob eukariot berperan penting dalam pembentukan bioflok di tambak budidaya pada lahan rawa payau. Bioflok menjadi pakan alami untuk budidaya ikan bandeng atau udang. Tujuan riset ini adalah mengidentifikasi mikrob eukariot rawa payau potensial untuk pengembangan kandidat bioflok di akuakultur rawa payau. Riset ini dilakukan dengan pendekatan kualitas air serta isolasi mikrob indigenous tambak dan rawa pasang surut untuk mendapatkan isolat mikrob potensial sebagai kandidat pembentuk bioflok. Sampling dilakukan secara subkomposit pada air dan sedimen di setiap inlet dan outlet pasang surut. Kualitas air tambak dan rawa pasang surut menunjukkan kondisi oksigen terlarut, salinitas, pH, dan amonia berada di luar batas optimum budidaya ikan bandeng, sehingga perlu dilakukan pengapuran dan pemupukan untuk memperbanyak mikrob indigenous-nya. Isolasi mikrob eukariot dari media pengayaan diperoleh jenis fungi akuatik yang multiseluler dan uniseluler, sedangkan mikroalga yang diperoleh yaitu dari golongan Bacillariophyta, Chlorophyta, dan Cyanophyta. Fungi yang dapat membentuk hifa dapat merangkai mikroalga dan khamir untuk membentuk flok. Susunan ini diharapkan dapat menjadi bahan bioflok yang berguna sebagai pengendali kualitas air sekaligus pakan alami bagi pengembangan budidaya di lahan rawa payau. Kata kunci: mikrob eukariot, bioflok, budidaya, tambak, rawa payau

Skrining Bakteri Kitinolitik Antagonis Terhadap Pertumbuhan Jamur Akar Putih (Rigidoporus lignosus) dari Rizosfir Tanaman Karet Muharni Muharni; Hary Widjajanti
Jurnal Penelitian Sains Vol 14, No 1 (2011)
Publisher : Faculty of Mathtmatics and Natural Sciences

Skrining bakteri kitinolitik dari rizosfir tanaman karet dilakukan dengan tujuan untuk memperoleh isolat bakteri kitinolitik yang antagonis terhadap pertumbuhan jamur akar putih (Rigidoporus lignosus). Bakteri diisolasi dari Perkebunan Karet Sembawa Kab. Banyuasin, dan skrining dilakukan dengan menggunakan media agar kitin dan uji antagonis terhadap jamur patogen dilakukan dengan menggunakan metode Chernin et al., (1995). Hasil penelitian didapatkan dua isolat bakteri kitinolitik yang antagonis terhadap pertumbuhan jamur akar putih (Rigidoporus lignosus). Identifikasi kedua isolat antagonis jamur akar putih ini tergolong kedalam genus Bacillus yaitu Bacillus sp. dan Bacillus apiarius

Deteksi Beberapa Bakteri Patogen pada Berbagai Jenis Ikan Konsumsi yang Laku Dijual di Pasaran Palembang Munawar Munawar; H. Widjajanti H. Widjajanti; E. Patriono E. Patriono; Sarno Sarno; A. Wulandari A. Wulandari
Jurnal Penelitian Sains No 1 (1996)
Publisher : Faculty of Mathtmatics and Natural Sciences

Penelitian menunjukkan bahwa ikan-ikan konsumsi yang terdiri atas 13 jenis dari ketiga pasar tempat pengambilan sampel tidak mengandung Salmonella, tetapi ditemukan satu jenis ikan, yaitu ikan gabus yang diambil dari salah satu pasar tempat pengambilan sampel mengandung V. Cholerae. Sedangkan hasil perhitungan angka kuman dari semua jenis ikan menunjukkan masih di bawah batas maksimum yang ditentukan oleh Departemen Kesehatan Republik Indonesia. Antara angka kuman dengan keadaan morfologi ikan menunjukkan terdapat hubungan yaitu keadaan morfologi ikan semakin jelek, angka kuman semakin meningkat.

Pengaruh MVA Gigaspora Margarita dan Bakteri Pelarut Fosfat Bacillus Megaterium Terhadap Tanaman Kedelai Hary Widjajanti
Jurnal Penelitian Sains No 10 (2001)
Publisher : Faculty of Mathtmatics and Natural Sciences

Mikoriza berperan dalam meningkatkan serapan unsur hara bagi tanaman terutama unsur P dan bakteri fosfat mampu melarutkan fosfat yang ada di dalam tanah sehingga menjadi tersedia bagi tanaman. Tujuan penelitian adalah untuk mengetahui pengaruh mikoriza vasikular arbuskular (MVA) Gigaspora margarita dan bakteri pelarut fosfat (BPF) Bacillus megaterium terhadap tanaman kedelai yang ditanam di tanah Ultisol. Penelitian dilakukan di kebun dan Laboratorium Mikrobiologi FMIPA Unsri dengan menggunakan rancangan acak lengkapdengan pola faktorial dengan faktor I adalah dosis inokulum MVA yang terdiri atas: tanpa inokulasi BPF, 5 ml suspensi BPF dan 10 ml suspensi BPF. Kombinasi perlakuan diulang tiga kali dan pengamatan diulang 2 (dua) kali yaitu: pada fase R5 dan pada fase produksi. Hasil penelitian menunjukkan bahwa fase R5 inokulasi ganda MVA dan bakteri pelarut fosfat meningkatkan persentase infeksi MVA, serapan P tanaman, dan berat kering tanaman. Pada akhir fase produksi inokulasi ganda MVA dan bakteri pelarut fosfat tidak berpengaruh terhadap berat kering biji per tanaman. Inokulasi ganda MVA dan bakteri pelarut fosfat meningkatkan kadar P tanah.

Pemanfaatan Rumput Fimbrisylis sp. dalam Proses Bioremediasi Tanah pada Berbagai Konsentrasi Limbah Minyak Bumi Sri Pertiwi E; Hary Widjajanti; Bambang Yudono; Hary Wahyudi
Jurnal Penelitian Sains Vol 14, No 1 (2011)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56064/jps.v14i1.129

Penelitian pemanfaatan rumput Fimbristylis sp dalam proses bioremediasi bertujuan untuk mengamati pengaruh pemanfaatan Fimbristylis sp. terhadap jumlah populasi bakteri dan persentase penurunan TPH pada proses bioremediasi. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) berpola faktorial yang terdiri dari 2 faktor. Faktor 1: kosentrasi limbah minyak bumi yang terdiri dari 5 taraf: C0:0%, C1:5% , C2:10%, C3:15% dan C4: 20% Faktor 2 Penggunaan rumput Fimbrisylis sp yang terdiri dari 2 taraf : R0 : Tanpa menggunakan rumput Fimbristylis sp. dan R1: menggunakan rumput Fimbristylis sp., diperoleh 10 kombinasi perlakuan, masing-masing diulang 3 kali. Hasil penelitian menunjukkan pemanfaatan Fimbristylis sp. meningkatkan jumlah populasi bakteri dan penurunan nilai Total Petroleum Hidrokarbon (TPH). Namun semakin banyak limbah minyak bumi yang ditambahkan maka pertumbuhan Fimbristylis sp. semakin rendah

Co-Authors A. Wulandari A. Wulandari Abdul Madjid Ade Bayu Saputra Agung Bimantara Agustina Alfia Rahma Kurniawati Ali Djamhuri Andika Puspita Dewi Angga Prasetya Mulya Aria Yanka Paula Putri Arum Setiawan Asiandu, Angga Puja Bambang Yudono Budiono Budiono Dade Jubaedah Dedik Budianta E. Patriono E. Patriono Eka Amelia Elfita Elfita Elfita Elfita Elfita Elisa Nurnawati Ervina Mukharomah Fitrya Fitrya Gusti Ayu Widayanti Handayani, Harminuke Eka Harminuke Eka Handayani Hary Wahyudi Heni Yohandini Hotly Maraulina Karta Sari Genti Laila Hanum Mardiyanto Mardiyanto Maria Lusia Marini Wijayanti Maulida Harwati Muharni Muharni Muharni Muharni Munawar Munawar Munawar Munawar Muslim Muslim Nabila Saraswati Neni Marlina Ni Luh Putu SR Nina Tanzerina Nita Aminasih NUNI GOFAR Nur Arifah Nurkayah, Nurkayah Putri, Amanda Rahmaniah Putri, Aria Yanka Paula Rahmawati Ridwan Salni Salni Salni Sarno . Sarno Sarno Singgih Tri Wardana Siti Alharzsa Siti Yuliani Sri Pertiwi E Syarifah Tanbiyaskur Vivi Hendra Sutandar Wuriesyliane Wuriesyliane

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