Chairul .
Treub Laboratory Botany Research Center Biology Research Center Institutions of Science Indonesia

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Heat stress effect and given of hexane extract jaloh bark (Salix tetrasperma Roxb) to cortisol level, triiodothyronine and hematology profile of broiler chickens ., Sugito; Manalu, W; Astuti, D.A; Handharyani, E; ., Chairul
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 3 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.3 KB) | DOI: 10.14334/jitv.v12i3.482

Abstract

Plasma concentration of cortisol and hematological profile on broiler chickens can be used as heat stress indicators. This study was conducted to investigate the effect of administration of n-hexane extract of jaloh bark (EHJ) on heat-stressed broiler chicken. Thirty broilers, aged 20 days (strain Cobb), were randomly divided into 5 groups. The first group was external control (tCP) i.e. chickens without heat stress and without EHJ administration. The second group was internal control (CP) representing chickens given heat stress without EHJ administration. The third, fourth, and  fifth groups were chickens given heat stress and EHJ administration at doses 5, 10, and 20 mg/kg BW, respectively symbolized as CP+EHJ5, CP+EHJ10, and CP+EHJ20. The experimental broilers were exposed to heat stress in constant temperature of 33 ± 1oC for 4 hours daily for 5 and 10 days. The EHJ was given 1 hour before temperature in the cage was raised and was given in daily basis.  The level of cortisol in feces were measured from feces collected 1-2 hours before  treatment, 3-4 hours after heat stress exposure, and 2-3 hours after heat stress termination. This research indicated that heat stress at temperature 33 ± 1oC during 2 and 4 hours increased cortisol  excretion in feces (P<0.05) and increased ratio H : L (P<0.10). The EHJ at dose of 10 mg/kg BW relatively gave more protection from heat stress impact on broilers. Key Words: Cortisol, Triiodothyronine, Heat Stress, Salix, Hematology
Histopathology of liver and kidney on broiler chicken exposed to heat stress and fed extract of Jaloh (Salix tetrasperma Roxb) ., Sugito; Manalu, W; Handharyani, E; ., Chairul
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.588 KB) | DOI: 10.14334/jitv.v12i1.566

Abstract

This research was conducted to study the effects of jaloh extract (Salix tetrasperma Roxb) on broiler chicken exposed to heat stress in constant temperature of 33 ± 1oC for 4 hours per day for 10 days. Fifteen chickens aged 20 days old (strain Cobb) were divided randomly into 5 groups. The first group was external control (tCP) of the chickens received neither heat stress nor jaloh extract. The second group was internal control (CP) representing chickens given heat stress without jaloh extract. The third, fourth, and fifth groups consisted of chickens given heat stress and 10 mg/kg BB of hexane (CP+Hek), ethyl acetate (CP+EtOAc), and ethanol (CP+EtOH) fractions, respectively. Heat stress and jaloh extract were given every day. Extract of jaloh were given 1 hour before temperature in the cage was raised. On 10th day of the experiment, 3 chickens were killed and necropted. Liver and kidney tissues from each treatment were analyzed for histological condition. The results indicated that heat stress can induce injury degeneration, necrosis, and infiltration of inflammation cells of liver and kidney. It is concluded that the hexane fraction of jaloh extract could protect the detrimental effects on heat stress. Key Words: Heat Stress, Extract Salix, Liver, Broiler Kidney
THE INFLUENCE OF DISTILATION TIME, POWDER SIZE, ON THE CINAMYLALDEHYDE CONTENT AND VOLATILE OIL Cinnamomum burmanii Nees ex. Bl. BARK Lisawati, Yovita; Sulianti, Sri Budi; ., Chairul
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 3, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.296 KB) | DOI: 10.14499/indonesianjpharm0iss0pp123-132

Abstract

The content of volatile oil in samples depends on the harvest time, distilation method, sample powder size, and location of sample cultured. The aim of this study was to investigate the influences of interval time of distillation and mesh size of bark powder, upon the concentration of major component (cinamylaldehyde). The distillate was collected with various interval times 1, 2, 3 and 4 hours and it was counted after the first drop of distillate. The mesh size of bark powder (4, 8 and 20 mesh) was set after the interval time of distillation had been studied. The major component was analysed using Gas Chromatography (GC). The results showed that the interval time of distillation at the second hour gave the highest volatile oil and cinamylaldehyde contents in the relative prosentage 0.19 % and 29.36 % respectively, compared with the first hour distilate, (0.16 %, 24.88 %), third hours distilate (0.14 %, 23.29 %) and fourth distilate (0.08 %, 17.65 %). The powder size of bark (8 mesh) gave the highest volatile oil and cinamylaldehyde contents (0.22 %, 32.81 %), compared to powder size of 4 mesh( 0.17 %, 20.45 %) and 20 mesh (0.19 %, 21.32 %). The results is still lower than standard of cinnamon oil, (cinamylaldehyde content 60 %). The lowering cinamylaldehyde content could be caused by low quality of material, collected from marketing method, early harvesting time, and storing for long.time.Key words: C. burmanii, bark, distilation time, cinamylaldehide
PENDAYAGUNAAN DUA JENIS ZINGIBERACEAE [C. mangga (temu mangga) dan K. angustifolia (kunci menir)] SEBAGAI SUMBER BAHAN IMUNOMODULATOR SECARA IN VITRO ., Praptiwi; ., Chairul
Jurnal Teknologi Lingkungan Vol. 11 No. 3 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (682.251 KB) | DOI: 10.29122/jtl.v11i3.1189

Abstract

Observation of imunomodulation properties of methanol extract derive (hexane,ethyl acetate, methanol/water) of Curcuma mangga (temu mangga) and Kaempferia angustifolia (kunci menir) had been carried out by in vitro assay. It was done by determining the phagocytised activity and capacity of macrophage cells of mice (Mus musculus) peritonium inducted with Staphyllococcus epidermidis. The result showed that each fraction had different phagocytised activity and capacity. Almost all of the fractions tested improved the activity (50–96 %) and capacity macrophage cells phagocytised compared to negative control (52 dan 502) and positive control (97 dan 1076). The positive control was 1000 ug Stimuno (Phyllanthus niruri extract), while the negative control was aquadest. The ethyl acetate fraction of Z. cassumunar (bangle) had highestphagocytised activity and capacity followed by C. mangga (temu mangga) and K. Angustifolia (kunci menir). It can be concluded that there were significant differences of phagocytised activity and capacity among fraction tested.Keywords : Zingiberaceae, Curcuma mangga, Kaempferia rotunda, Zingibercassumunar, immunomodulator, phagocytised, macrophage cells.