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SKRINING DAN ANALISIS KLT-BIOAUTOGRAFI SENYAWA ANTIMIKROBA BEBERAPA EKSTRAK SPONS ASAL PERAIRAN LAUT PULAU BARRANG LOMPO, SULAWESI SELATAN Yulianty, Risfah; Rante, Herlina; Alam, Gemini; Tahir, Akbar
Majalah Obat Tradisional Vol 16, No 2 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.514 KB) | DOI: 10.14499/mot-TradMedJ16iss2pp88-94

Abstract

Spons merupakan salah satu invertebrata filum Porifera yang menghasilkan senyawa aktif dengan berbagai variasi struktur dan salah satu aktivitas biologinya adalah sebagai antimikroba. Penelitian ini bertujuan untuk mengetahui ekstrak spons yang dapat menghambat pertumbuhan mikroba dan potensi senyawa aktif antimikroba dari spons asal perairan Pulau Barrang Lompo. Mikroba uji yang digunakan adalah Escherichia coli, Salmonella typhii, Staphylococcus aureus, dan Candida albicans. Bahan uji diperoleh dengan maserasi 14 buah sampel spons dengan metanol yang dilanjutkan dengan partisi berturut-turut menggunakan kloroform dan metanol, selanjutnya dilakukan uji KLT-bioautografi terhadap ekstrak yang aktif. Hasil penelitian menunjukkan bahwa ekstrak metanol dari sampel spons dengan kode BRLP-009 dan BRLP-010 mempunyai aktivitas paling potensial sebagai antimikroba.
Skrining Senyawa Antiinfeksi dari Spons yang Dikoleksi dari Bunaken, Manado Astuti, Puji; Alam, Gemini; Pratiwi, Sylvia; Hertiani, Triana; Wahyuono, Subagus
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 2 (2003): June 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.243 KB) | DOI: 10.24002/biota.v8i2.2883

Abstract

The emergence of drug resistant and multidrug-resistant microbes as well as the lack of any current chemotherapy augmented the necessity to search for new and better anti-infective drug.. This study was aim to screen potential antiinfective extracts of sponges collected from Bunaken, Manado and to report on their antibacterial and antifungal properties. Testing for antiinfective agents was conducted using dilution method. Nutrient Agar was used as the testing media and nutrient broth for the inoculation of microorganisms. Staphylococcus aureus, Escherichia coli and Salmonella typhi were used as the testing bacteria and Candida albicans as the testing fungi. Chloramphenicol was used as positive control for anti bacterial activity and Ketokonazol for antifungi. The two acetone extracts tested (MD-01aceton and MD-02aceton), all of them showed inhibition activities. Following partition with chloroform and methanol, all chloroform extracts inhibited the growth of both bacteria and fungi. These extracts then are considered to be potential candidates for further isolation and characterization as antiinfective agents.
Pengaruh Umur Dan Pekerjaan Ibu Terhadap Kandungan Antioksidan Asi Enggar; Alam, Gemini; Maddeppungeng, Martira
Jurnal Bidan Cendrawasih Palu Vol 1 No 1 (2019): MARET
Publisher : Akademi Kebidanan Palu Sulawesi Tengah

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Antioxidant is a compounds which can protect a baby from an oxidative stress and free radical hazard. Breast milk contains antioxidant and represents is the best food for a baby. This research animed at investigating the effect of women’s age and occupation on the breast milk antioxidant. The research used the cross sectional design with of 24 samples of the breastfeeding women who were selected using the purposive sampling technique. The respondents who were taken their breast milk samples were in healthy condition and were asked to become volunteers by contributing their breast milk in the sake of the research. The samples taken were the women who breastfed and had the babies ≤ 6 months old and fulfilled the inclusive and exclusive criteria. Oneway ANOVA analysis was used to perceive the effect of the women’s age and occupation on breast milk antioxidant. The research result indicates that the respondents’ age based on statistic test results is obtained the score of p= 0,563 ˃α=0,05 meaning that there is not significant effect between women’s age and breast milk antioxidant. The Respondent's occupation indicated the score of p= 0,055 ˃α=0,05 which means that there is no significant effect between the occupation and breast milk antioxidant. It is concluded that there is no effect of the women’s age and occupation on breast milk antioxidant. It is expected that the women who work outdoors can still give the breast milk. The women who can not give their breast milk directly can still give their breast milk by way squeezing.