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Imunoekspresi CK-7 dan CK-20 pada Karsinoma Musinosum Ovarium Primer di RSUP Dr. Hasan Sadikin Bandung Lili Sumarni; Hasrayati Agustina; Afiati Afiati
Majalah Patologi Indonesia Vol 27 No 1 (2018): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Abstract

LatarbelakangKarsinoma musinosum ovarium (KMO) merupakan subtipe karsinoma ovarium, yang sering mempunyai prognosis buruk. Ada dua bentuk KMO yang sering ditemukan, yaitu bentuk primer (KMOP) dan sekunder atau metastasis (KMOS). Untuk membedakan terapi tumor primer (KMOP) dan sekunder/metastasis perlu dilakukan pemeriksaan menggunakan CK-7 dan CK-20. Penelitian ini bertujuan untuk menganalisis imunoekspresi CK-7 dan CK-20 pada KMO.MetodePenelitian ini menggunakan desain penelitian potong lintang dengan data deskriptif retrospektif pada kasus KMO yang secara klinis didiagnosis tumor primer ovarium di RSUP dr. Hasan Sadikin Bandung periode Januari 2013 sampai dengan Juni 2016. Pada seluruh kasus dilakukan pemeriksaan histopatologi dan imunohistokimia CK-7 dan CK-20 dan dilihat parameter klinis yaitu ukuran dan lateralitas. KMOP didiagnosis bila ukuran lebih dari dari 10 cm dan unilateral, sedangkan KMOS bila ukuran kurang dari 10 cm dan bilateral.HasilHasil penelitian ini menunjukkan dari 49 kasus KMO, sebanyak 17 kasus (34,7%) adalah karsinoma musinosum ovarium sekunder (KMOS) {CK-7(-), CK-20(+)} dan 32 kasus (65,3%) adalah KMOP {CK-7(+), CK-20(-)}. Seluruh kasus KMOP (100%) dan sebagian besar kasus KMOS (88,2%) berukuran lebih besar dari 10 cm. Tidak terdapat hubungan antara ukuran tumor (p=0,29 ) dan lateralitas (p=0,94) yang signifikan dengan KMOP dan KMOS.KesimpulanPulasan imunohistokimia (IHK) CK-7 dan CK-20 dapat digunakan untuk menegakkan diagnosis KMOP.
Characteristics of Thyroiditis Patients in Dr. Hasan Sadikin General Hospital in 2009–2013 Sri Maryanti; Hasrayati Agustina; Mifatuhrachman Mifatuhrachman
Althea Medical Journal Vol 3, No 1 (2016)
Publisher : Faculty of Medicine Universitas Padjadjaran

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Abstract

Background: It is reported that thyroid diseases affect around 200 milion people in the world. One of them is thyroiditis that may cause the risk of cancer. Moreover, thyroiditis can also cause hormonal disorders, such as hypothyroid and hyperthyroid. It is assumed that thyroiditis has distinctive clinical characteristics. The aim of this study was to evaluate the characteristics of thyroiditis based on age, gender, location, and clinical features of the patient.Methods: The study was conducted using descriptive-retrospective method. The data were collected from patients’ medical records through total sampling from January 2009 to Desember 2013 in Dr. Hasan Sadikin General Hospital, Bandung.Results: It was found 35 cases of thyroiditis. Based on the histopathological type, the most frequently found thyroiditis was Hashimoto’s thyroiditis. Based on age, thyroiditis mostly affected people at age 41–60 years old. Based on the gender, thyroiditis mostly affected female and the location of lesions were bilateral. The majority clinical features of patients were hypothyroid in Hashimoto’s thyroiditis, hyperthyroid in acute thyroiditis, and normothyroid in sub-acute granulomatous thyroiditis.Conclusions: Based on age, gender, and the location, the majority type of thyroiditis did not show specific characteristics. However, thyroiditis showed specific characteristics based on the clinical features of patient. [AMJ.2016;3(1):49–53]DOI: 10.15850/amj.v3n1.701
Perbandingan Imunoekspresi p53 dan Caspase-3 Pada Mola Hidatidosa Parsial, Mola Hidatidosa Komplit, dan Koriokarsinoma Odilia Dos Reis; Hasrayati Agustina; Abdul Hadi Hassan; Bethy S Hernowo
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol. 2 No. 2 (2014): JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.405 KB) | DOI: 10.22437/jmj.v2i2.2713

Abstract

ABSTRACT Background: Partial hydatidiform mole, complete hydatidiform mole and choriocarcinoma are gestational trophoblastic diseases which have high incidence in Indonesia. Most complete hydatidiform mole can transform into choriocarcinoma , but pathogenesis of the transformation  is remain unknown. Apoptosis plays an important role in the pathogenesis of human malignancy. p53 and caspase - 3 play a role in apotosis . The aim of this study is to evaluate the p53 and caspase-3  immunoexpression in partial hydatidiform mole, complete hydatidiform mole and choriocarcinoma Methods: This is an observational analytic cross  sectional  study, conducted on 30 cases of gestational trophoblastic diseases consisted of 10 cases of partial hydatidiform mole, 10 cases of complete hydatidiform mole and 10 cases of choriocarcinoma. Immunohistochemical  examination of p53 and Caspase-3 were  performed on all cases. Result : The result showed significant difference between the p53 immunoexpression in partial hydatidiform mole and choriocarcinoma (p = 0.002), there was no significant p53 immunoexpression difference between complete hydatidiform  mole and choriocarcinoma (p = 0.121), there was no caspase-3 immunoexpression difference between partial hydatidiform mole and choriocarcinoma (p = 0.160) and there was no caspase-3 immunoexpression difference between complete hydatidiform mole and choriocarcinoma  (p = 0.361). p53 Immunoexpresion was higher in the case of complete hydatidiform mole and choriocarcinoma but tend to be lower in partial hydatidiform mole. Conclusion : In conclusion, p53 may play a role in the pathogenesis of complete hydatidiform mole and choriocarcinoma, but has a lesser role in the pathogenesis of partial hydatidiform mole. Caspase-3 might not play a role in the pathogenesis of hydatidiform mole transformation into choriocarcinoma. Keywords: complete hydatidiform mole, partial hydatidiform mole, choriocarcinoma, p53, caspase-3.
PENGARUH LATIHAN FISIK AEROBIK DENGAN INTENSITAS SEDANG TERHADAP KONDISI MAKROSKOPIK DAN GAMBARAN HISTOPATOLOGI MIOKARD MENCIT BALB/C Niswan Helja Batubara; Nova Sylviana; Hasrayati Agustina; Hadaral Hudanul Qolbi
JURNAL ILMU FAAL OLAHRAGA INDONESIA Vol 2, No 1 (2019)
Publisher : PAIFORI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1184.731 KB) | DOI: 10.51671/jifo.v2i1.83

Abstract

Cardiovascular diseases is one of the non-communicable diseases that has been the leading cause of death compared to other causes. Actually, cardiovascular diseases can be prevented with avoiding the risk factors and lifestyle improvements such as doing physical exercise. Useful physical exercise according to according to The American College of Cardiology/American Heart Association (ACC/AHA) is a moderate intensity aerobic exercise conducted 30 minutes in time for 5 days a week. This research aims to study the effects of moderate intensity aerobic exercise on macroscopic conditions, histopathologic features of the myocardium of mice. This research using 35 male balb/c mice devided to 3 groups (control, physical activity, exercise). Variables in this research included duration of physical activity and exercise, macroscopic conditions, histopathologic features of the myocardium of balb/c mice. After 10 and 28 days of exposure, the heart were harvested. The macroscopic conditions, histopathologic features of the myocardium were examined. The weight of heart in physical activity group was greater than control group (p=0.009). Similarly, the weight of heart exercise group was also greater compared to control group (p=0.013). The histopathologic features in physical activity and physical exercise groups showed pathological features of myocardial infarction (p=0.242). While in the control group did not show pathological features. Aerobic physical activity moderate intensity increase the risk of pathological condition of heart in the form of myocardial infarct through the macroscopic conditions and histological features of myocardium. Meanwhile, exercise with moderate intensity affect the physiological hypertrophy of the myocardium.
Korelasi antara Imunoekspresi LMP-1 Virus Epstein-Barr dengan Respon Kemoterapi CHOP pada Limfoma Maligna Non-Hodgkin Tipe Diffuse Large B Cell. Inas Susanti; Hasrayati Agustina; Bethy S Hernowo
Majalah Patologi Indonesia Vol 23 No 2 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Abstract

Background Diffuse large B cell lymphoma is the most common type of B-cell non-Hodgkin malignant lymphoma. Epstein-Barr virus (EBV) has been shown to contribute to the development of this tumor especially in the elderly patient (above 50 years). The chemotherapeutic agents CHOP (Cyclophospamide, Hydroxydaunorubicin, Oncovin, Prednison) are standard treatment for non-Hodgkin lymphoma patients, but approximately 30% cases shows non-respone with standard chemotherapy and undergoes progressive disease until death. There are no accurate predictor factors in predicting CHOP chemotherapy respone in patients with DLBCL. The aim of this study is to determine the role of LMP-1 in predicting CHOP chemotherapy respone in patients with DLBCL. Methods This study was done retrospectively in 34 cases of DLBCL. The samples were obtained from the Anatomic Pathology Department of Dr. Hasan Sadikin Hospital from January 2009 to Desember 2011. All cases were reviewed for histopathological diagnoses based on the WHO Classification of 2001 and the specimens were stained with specific antibodies against CD 20 (for diagnoses DLBCL) and LMP-1. The data were analized using rank Spearman’s correlation test. Results This study showed that 31 cases (91.18%) were LMP-1 positive, only 3 cases (8.82%) were negative. Three cases of negative LMP-1 had a good respone to chemotherapy, whereas among 31 positive cases LMP-1 showed good respone to chemotherapy in 21 cases and the remaining 10 cases had a poor respone to chemotherapy. There was no correlation between EBV-LMP-1 immunoexpression with CHOP chemotherapy respone in DLBCL (p=0.436). Conclusion There was no correlation between EBV-LMP-1 immunoexpression with CHOP chemotherapy respone in DLBCL. Key words: CHOP, DLBCL, EBV, LMP-1, non-Hodgkin lymphoma.
Validitas Pemeriksaan Imunositokimia HMGA2 dalam Penegakan Diagnosis Nodul Jinak dan Ganas Tiroidpada Sediaan Biopsi Aspirasi Jarum Halus Yenni Wisudarma; Hasrayati Agustina; Sri Suryanti; Bethy S. Hernowo
Majalah Patologi Indonesia Vol 26 No 1 (2017): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Abstract

Latar belakang Tindakan biopsi aspirasi jarum halus (BAJAH) merupakanpemeriksaan preoperatif baku yang sederhana dan relatif lebih murah dalam penegakan diagnosis karsinoma tiroid, tetapi diagnosis sitologi BAJAH tersebut tidak selalu mudah. Penelitian ini bertujuan untuk mengetahui validitas pemeriksaan imunositokimia HMGA2 dalam penegakan diagnosis sediaan BAJAH nodul tiroid jinak, ganas dan indeterminate. Metode Penelitian ini merupakan penelitian uji diagnostik yang dilakukan terhadap 62 kasus nodul tiroid yang terdiri dari 18 kasus karsinoma papiler,13 kasus nodul folikular jinak dan 31 kasus indeterminate (27 kasus follicular neoplasmdan 4 kasus suspicious for papillary carcinoma) yang didiagnosis tahun 2011-2014 dan telah dilakukan pemeriksaan histopatologi pasca operasi sebagai konfirmasi diagnosis. Pemeriksaan imunositokimia HMGA2 dilakukan terhadap semua kasus dengan metode cell transfer. Hasil Akurasi, sensitivitas, spesifisitas, nilai duga positif dan nilai duga negatif pemeriksaan imunositokimia HMGA2 dalam diagnosis nodul tiroid masing-masing adalah 85,5%, 79,4%, 92,8%, 93,1% dan 78,8%. Sedangkan untuk kasus indeterminate, akurasi, sensitivitas, spesifisitas, nilai duga positif dan nilai duga negatif pemeriksaan imunositokimia HMGA2 adalah 77,4%, 68,7%%, 86,7%, 84,6% dan 72,2%. Kesimpulan Pemeriksaan imunositokimia HMGA2 relatif spesifik dan sensitif dalam menegakkan diagnosis preoperatif nodul tiroid termasuk pada kasus indeterminate. Kata kunci : biopsi aspirasi jarum halus, HMGA2, indeterminate, nodultiroid.
Hubungan Imunoekspresi CD44 dengan Metastasis Karsinoma Papilari Tiroid Yasni Iryani Widiasih; Herry Yulianti; Hasrayati Agustina; Bethy S Hernowo
Jurnal Kedokteran Universitas Lampung Vol 3, No 1 (2019): JK Unila
Publisher : Fakultas Kedokteran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jk unila.v3i1.2202

Abstract

Karsinoma tiroid (KT) merupakan keganasan endokrin yang paling sering terjadi dan karsinoma papilari tiroid (KPT) adalah tipe histopatologis terbanyak mencapai 80%. KPT mempunyai perangai yang baik dengan pertumbuhan yang lambat. Namun ditemukan KPT dengan perangai yang agresif, ditandai dengan metastasis ke KGB lebih dari 50% kasus. Cluster of Differentiation 44 (CD44) merupakan penanda imunohistokimia yang berperan dalam memprediksi sifat agresif tumor ganas payudara. Namun peran CD44 pada KPT belum diketahui dengan pasti. Tujuan penelitian ini adalah mengetahui hubungan antara imunoekspresi CD44 dengan metastasis pada KPT.Penelitian ini merupakan penelitian cross sectional terhadap kasus KPT yang didiagnosis secara histopatologis di Departemen Patologi Anatomi RSUP Dr Hasan Sadikin Bandung tahun 2012-2017. Jumlah sampel sebanyak 60 sampel yang terdiri dari 30 kasus KPT non metastasis dan 30 kasus KPT metastasis, keseluruhan sampel dilakukan pulasan imunohistokimia CD44 .Imunoekspresi CD44 pada kelompok metastasis terekspresi lemah pada 6 kasus (20,0%) dan terekspresi kuat pada 24 kasus (80,0%), sedangkan pada kelompok non metastasis terekspresi lemah pada 13 kasus (43,3%) dan terekspresi kuat pada 17 kasus (56,7%) dengan nilai p value 0,052. Hasil dari penelitian ini adalah tidak terdapat hubungan yang signifikan secara statistik antara imunoekspresi CD44 terhadap metastasis. Berdasarkan hasil penelitian tersebut dapat disimpulkan bahwa CD44 tidak berperan di dalam metastasis dan tidak dapat dijadikan penanda untuk memprediksi metastasis pada kasus KPT serta diperlukan penelitian lanjutan dengan pemilihan penanda yang lebih selektif.Kata Kunci : agresif, CD44, KPT, metastasis