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Efek Pemberian Madu terhadap Lesi Hepar Maternal Mencit Terpapar Monosodium Glutamate (MSG) selama Masa Kehamilan Sufitni -; Delyuzar -; Emita Sabri
Majalah Patologi Indonesia Vol 22 No 2 (2013): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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ABSTRAK Latar belakang Monosodium glutamate (MSG) merupakan zat aditif makanan yang masih kontroversial. Konsumsi MSG yang tinggi meningkatkan metabolisme glutamat, dan radikal bebas yang berbahaya bagi tubuh. Salah satu antioksidan yang dapat menangkal radikal bebas adalah madu. Penelitian ini bertujuan untuk menguji efek madu terhadap lesi hepar induk mencit yang dipicu oleh pemberian MSG pada saat kehamilan. Metode Penelitian bersifat eksperimental murni dengan rancangan acak lengkap (RAL), yang terdiri atas 5 kelompok, yaitu 1 kelompok kontrol (K) dan 4 kelompok perlakuan (P1-P4). Kelompok kontrol hanya diberi akuades selama kehamilan 0-18 hari. Selama kehamilan 0-18 hari, P1 diberi MSG 4 mg/g BB/hari, P2 diberi madu 0,15 g/20 g BB/hari, dan P4 diberi MSG 4 mg/g BB/hari dan madu 0,15 g/20 g BB/hari. P3 diberi MSG 4 mg/g BB/hari pada kehamilan 0-7 hari, dilanjutkan pemberian madu 0,15 g/20 g BB/hari pada kehamilan 8-18 hari. Pada usia kehamilan 18 hari, mencit dikorbankan dengan cara dislokasi leher dan dilakukan nekropsi pengambilan hepar. Hasil Pemberian MSG dengan dosis 4 mg/g BB bersifat hepatotoksik dibanding kontrol (p= 0,041). Pemberian madu mampu mengurangi efek hepatotoksik MSG. Kesimpulan Madu mampu mengurangi efek hepatotoksik dari paparan MSG. Kata kunci : Kehamilan, lesi hepar, madu, monosodium glutamate (MSG). ABSTRACT Background Monosodium glutamate (MSG), a food additive that is still a controversial. High consumption of MSG will increase glutamate metabolism and free radical. Honey as antioxidant can remove free radical. The purpose of study was to determine the effects of honey therapy against hepatic lesions induced by MSG during pregnancy Methods The study was a true experimental with a completely randomized design (CRD), which consists of a control group (K) and four treatment groups (P1-P4). The control group (K) was given distilled water during gestation days 0-18. During of 0-18 days of gestation, P1 were given MSG 4 mg/g body weight (BW)/day, P2 were given honey 0.15 g/20 g BW/day, and P4 were given MSG 0.15 g/20 g BW/day and honey 4 mg/g BW/day. P3 were given MSG 4 mg/g BW/day on days 0-7 of pregnancy, and continued provision of honey 0.15 g/20 g BW/day on days 8-18 of pregnancy. At gestational age 18 days, each treatment and control mice were killed by cervical dislocation and then dissected for liver. Results Giving of high doses of MSG to mice during pregnancy, give hepatotoxic effect compare control (p=0.041). Giving honey can reduce hepatotoxic of MSG effect. Conclusion Honey was able to reduce the hepatotoxic effects of MSG administration. Key words: Hepatic lesion, honey, monosodium glutamate (MSG), pregnancy.
Perbedaan Ekpresi Imunositokimia Thyroid Transcription Factor-1 (TTF-1) pada Adenokarsinoma dan Karsinoma Sel Skuamosa dari Sikatan Bronkus Kanker Paru Lita Feriyawati; Nadjib Dahlan Lubis; Delyuzar -
Majalah Patologi Indonesia Vol 24 No 1 (2015): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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ABSTRAK Latar belakang Karsinoma bukan sel kecil (KBSK) merupakan 85% keganasan dari seluruh kanker paru. Dalam beberapa tahun terakhir adenokarsinoma merupakan tipe KBSK yang paling dominan. Pemeriksaan sitologi sikatan bronkus dengan pewarnaan rutin papanicolaou pada beberapa keadaan mempunyai keterbatasan, terlebih pada beberapa jenis tumor paru dengan gambaran sel yang mirip. Thyroid transcription factor-1 (TTF-1) diekspresikan pada adenokarsinoma paru dan karsinoma sel kecil paru. Pemeriksaan imunositokimia TTF-1 dengan prosedur yang efisien dan efektif sebagai alat bantu pemeriksaan sitologi sikatan bronkus dapat meningkatkan keakuratan diagnosa sitologi kanker paru. Tujuan penelitian ini adalah untuk membedakan adenokarsinoma dan karsinoma sel skuamosa (KSS) dari sikatan bronkus kanker paru melalui tampilan imunositokimia TTF-1. Metode Penelitian ini merupakan penelitian analitik dengan rancangan potong lintang pada 45 sampel sitologi sikatan bronkus yang terdiri dari adenokarsinoma sebanyak 77,78% (n=35) dan KSS sebanyak 22,22% (n=12). Dilakukan pemeriksaan imunositokimia TTF-1 menggunakan antibodi TTF-1 rabbit polyclonal (klon ab136633) dengan metode labelled streptavidin biotin immunoperoxidase complex langsung pada slaid yang sebelumnya telah diwarnai dengan papanicolaou. Hasil Pada penelitian ini sebanyak 62,9% (n=22) adenokarsinoma menunjukkan tampilan positif dengan imunositokimia TTF-1, ekspresi kuat 36,3% (n=8), ekspresi sedang 50% (n=11), dan ekspresi lemah 13,6% (n=3), serta tampilan negatif 37,1% (n=13). Sampel KSS menampilkan hasil positif dengan imunositokimia TTF-1 sebanyak 30% (n=3) dengan ekspresi kuat serta tampilan negatif sebanyak 70% (n=7). Hasil uji hubungan antara hasil imunositokimia TTF-1 dengan ekspresi pada adenokarsinoma dan KSS menggunakan uji Fisher’s exact menunjukkan p-value = 0,083. Kesimpulan Pada penelitian ini didapati tampilan intensitas yang beragam pada hasil imunositokimia TTF-1 pada jenis adenokarsinoma dan KSS, dan menurut perhitungan statistik tidak bermakna. Kata kunci: adenokarsinoma, kanker paru, karsinoma sel skuamosa, sikatan bronkus, thyroid transcription factor-1. ABSTRACT Background Non-small cell carcinoma (NSCC) is 85% of all lung cancer malignancy. In recent years, adenocarcinoma is the most dominant type of NSCC. Bronchial brushings cytology examination with routine Papanicolaou staining have limitations especially to distinguish between some types of lung tumors which have similar pattern. Thyroid transcription factor-1 (TTF-1) is a transcription factor that normally found in adult thyroid and lung tissue, expressed in lung adenokarsinoma and small cell lung carcinoma. Immunocytochemistry examination TTF-1 is an efficient and effective procedures as aids bronchial brushings cytology examination that can improve the accuracy of cytological diagnosis of lung cancer. The objective of the research is to determine the differences between adenocarcinoma and squamous cell carcinoma (SCC) from bronchial brushings in lung cancer by the expression of immunocytochemistry TTF-1. Methods A cross sectional analytic study was performed at 45 brushings cytology samples consisted of 77.78% (n=35) adenocarcinoma and 22.22 % (n=12) SCC Immunocytochemistry examination using rabbit polyclonal (clone ab136633) TTF-1 antibody was perfomed by immunoperoxidase labeled streptavidin biotin complex methode directly on slides previously stained with Papanicolaou. Results In this study, 62.9% (n=22) adenocarcinoma were positive for TTF-1 with 36.3% (n=8) gave strong expression, 50% (n=11) gave moderate expression, 13.6% (n=3) gave weak expression and showed negative as 37.1% (n=13). SCC were positive for TTF-1 as 30% (n=3) with overall gave strong expression and showed negative results as 70% (n=7). The relationship between TTF-1 expression in adenocarcinoma and SCC by using Fisher 's exact test showed p-value=0.083 Conclusion This study showed that there were not differences of TTF-1 immunocytochemistry intensity between adenocarcinoma and SCC. Key words : adenocarcinoma, bronchial brushings, lung cancer, squamous cell carcinoma, thyroid transcription factor-1.
35 Vol. 24 No. 1 Januari 2015 Ekspresi IFN-γ dan IL-4 pada Tumor Jinak dan Ganas Epitelial Ovarium Jenis Serosum dan Musinosum Ainun Mardiah; Nadjib Dahlan Lubis; Delyuzar -
Majalah Patologi Indonesia Vol 24 No 1 (2015): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Abstract

ABSTRAK Latar belakang Tumor serosum dan musinosum merupakan jenis tumor epitelial ovarium. IFN-γ berhubungan dengan tumor epitelial ovarium melalui aktivitas anti proliferasi, walaupun pada keadaan tertentu IFN- γ mempunyai efek apoptosis terhadap sel tumor ovarium manusia. Terapi IFN-γ mempunyai aktivitas anti dan protumor, tergantung pada keadaan selular, lingkungan mikro, dan konteks molekul. Sitokin IL-4 berperan sebagai anti apoptosis. Tujuan penelitian ini adalah melihat perbedaan ekspresi IFN-γ dan IL-4 pada tumor jinak dan ganas epitelial ovarium tipe serosum dan musinosum. Metode Penelitian ini merupakan penelitian observasional analitik dengan pendekatan potong lintang dari 40 sampel jaringan tumor jinak dan ganas serosum dan musinosum dengan jumlah yang sama. Dilakukan pemeriksaan imunohistokimia dengan antibodi IFN-γ dan IL-4. Hasil Pada penelitian ini IL-4 menunjukkan ekspresi positif sebanyak 60% (n=6) dan pada tumor jinak serosum, sedangkan pada tumor ganas serosum ekspresi positif 90% (n=9). Pada tumor jinak musinosum IL-4 menunjukkan ekspresi positif 40% (n=4), sedangkan pada tumor ganas musinosum seluruhnya menunjukkan ekspresi positif (100%). IFN-γ menunjukkan ekspresi positif sebanyak 50% (n=5) pada tumor jinak serosum, sedangkan tumor ganas serosum menunjukkan ekspresi positif 40% (n=4). Pada tumor jinak musinosum IFN-γ menunjukkan ekspresi positif 60% (n=6), sedangkan pada tumor ganas musinosum menunjukkan ekspresi positif 80% (n=80). Kesimpulan Tidak terdapat perbedaan bermakna ekspresi IFN-γ dan IL-4 pada tumor jinak dan ganas serosum dan musinosum. Kata kunci : imunohistokimia, tumor epitelial ovarium, tumor musinosum ovarium, tumor serosum ovarium. ABSTRACT Background Serous and mucinous tumors were variant of epithelial ovarian tumors. IFN-γ associated with anti-proliferative activity of the tumor, but in the spesific situation IFN-γ has anti apoptosis effect against the ovarian tumor cells. IFN-γ treatment was protumorigenic and anti tumorigenic activities were dependent on the cellular, microenvironment, and/or molecular context. The cytokines IL-4 acts as an anti-apoptotic. The purpose of this study was to view the difference expression of IFN-γ and IL-4 in benign and malignant serous and mucinous epithelial ovarian tumors. Methods This study is a observational analytic assesment with cross-sectional approach in 40 samples of benign and malignant serous and mucinous tumors. Immunohistochemistry with IFN-γ and IL-4. Results In this study the expression of IL-4 was positive in 60% (n=6) in the benign serous tumors, whereas in the malignant serous tumors was positive in 90% (n=9). In the benign mucinous tumors the expression of IL-4 was positive in 40% (n=4), whereas in the malignant mucinous tumors was entirely positive (100%). Expression of IFN-γ was positive in 50% (n=5) in the benign serous tumors, whereas in the malignant serous tumors was positive in 40% (n=4). In the benign mucinous tumors the expression of IFN-γ was positive in 60% (n=6), whereas the malignant mucinous tumors was positive in 80% (n=80). Conclusion There was not difference expression of IFN-γ and IL-4 in benign and malignant serous and mucinous tumors. Key words: epithelial ovarian tumor, immunohistochemistry, mucinous tumor, serous tumor.