Garuda - Garba Rujukan Digital

Antibacterial Activity of Marine Bacteria Associated with sponge Aaptos sp. against Multi Drugs Resistant (MDR) strains Ocky Karna Radjasa; Duhita Sinidhikaraning Kencana; Agus Sabdono; Rory Anthony Hutagalung; Endang Sri Lestari
Jurnal Matematika & Sains Vol 12, No 4 (2007)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

The improper and uncontrolled uses of antibiotics against pathogenic bacteria have resulted in the occurrence of MultiDrugs Resistant (MDR) strains. There is now an urgency to find alternative antibiotics to combat the MDR strains.Sponge associated microorganisms are among the most interesting and promising marine natural product sources whichproduce polyketide and non ribosomal peptide products with various biological activities. In this study, marine bacteriawere isolated from sponge Aaptos sp. collected from North Java Sea, and were screened for antibacterial activityagainst MDR strains. Three out of 64 bacterial isolates were successfully screened and were found to be active againstMDR strains, in which two isolates (SPA1 and SPA5) were active against resistant strain Escherichia coli and one isolate(SPA21) against resistant strain Proteus sp., respectively. These active isolates were also capable of amplifying NRPS(Non Ribosomal Peptide Synthetases) gene fragments necessary for the biosynthesis of non ribosomal peptides. Themolecular identification based on partial 16S DNA nukleotide sequences indicates that the active isolates areHalomonas aquamarina, Alpha proteobacterium, and Pseudoalteromonas luteviolacea.

Efektivitas ekstrak etanol daun ungu (Gratophyllum pictum (L.)) untuk menurunkan kadar TNF-Î± dan NO Tjahjani, Nur Patria; Kristina, Tri Nur; Lestari, Endang Sri
Pharmaciana Vol 6, No 2 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Ethanol extract of Gratophyllum pictum L (G. pictum L). leaves contain phytochemical compounds such as alkaloids, tannins and flavonoids, which have antibacterial activity against Staphylococcus aureus. This study aims to prove the effectiveness ofÂ ethanol extract ofÂ G. pictum L. to reduce the level of TNF-Î± and NO in Swiss mice infected with S. aureus.The study treated 30 Swiss mice divided into 5 groups, consist of: K (-) normal mice ; K (+) mice were infected with S. aureus ; P1, P2, P3 were infected with S. aureus and given the ethanol extract of G. pictum leaves,75,150, and 300 mg/kgBW/day for 7 days . Mean levels of TNF-Î± of group K(-), K (+), P1, P2, P3 were 300.29; 550.29; 390.34; 517.67; 327.55 pg / mL respectively. One Way ANOVA test showed that there were significant differences between all treatment groups (p = 0.001), LSD Post Hoc test revealed P1 and P3 groups different significantly with K (+) with p= 0.013 and p = 0.001. Mean levels of NO of group K (-), K (+), P1, P2, P3 were 1.32; 3.56; 2.50; 2.64; and 1.56 Î¼M/mL respectively. Kruskal WallisTest showed that the levels of NO not significantly different, although NO levels at 300 mg/kgBW/day similar with to the levels of NO group K(-). Spearman correlation test showed that there were correlations between TNF-Î± and NO with the variation results on each treatment groups. Ethanol extract ofÂ G. pictum leaves at 300 mg/kgBW/day for 7 days is regarded as the effective dose to reduce TNF-Î± and NO levels in Swiss mice infected by S. aureus.

PENINGKATAN HASIL BELAJAR IPA MELALUI PENERAPAN MODEL PEMBELAJARAN OUTDOOR CLASS PADA SISWA TUNANETRA KELAS XI SLB N 1 BANTUL YOGYAKARTA Sri Lestari, Endang
Jurnal Guru Dikmen dan Diksus Vol. 2 No. 2 (2019): Juli-Desember 2019
Publisher : Direktorat Guru dan Tenaga Kependidikan Pendidikan Menengah dan Pendidikan Khusus

Penelitian ini bertujuan meningkatkan hasil belajar IPA pada siswa tunanetra melalui penerapan Model Pembelajaran Outdoor Class di SLB N 1 Bantul Yogyakarta. Penelitian menggunakan pendekatan penelitian tindakan kelas. Desain penelitian menggunakan Model Kurt Lewin dengan dua siklus dimana setiap siklus terdiri dari empat tahapan kegiatan, yaitu perencanaan, pelaksanaan observasi dan refleksi. Masing-masing siklus diselesaikan dalam dua pertemuan. Penelitian melibatkan siswa tunanetra kelas XI di SLB N 1 Bantul Tahun Pelajaran 2016/2017. Teknik pengumpulan data dilakukan melalui tes, wawancara, observasi dan dokumentasi. Analisis data dilakukan dengan pendekatan deskriptif kuantitatif. Berdasarkan hasil penelitian dapat disimpulkan bahwa hasil belajar IPA siswa tunanetra kelas XI di SLB N 1 Bantul dapat ditingkatkan melalui penerapan Model Pembelajaran Outdoor Class. Hal ini dapat dilihat dari rata-rata peningkatan prosentase ketuntasan belajar siswa

Efektivitas ekstrak etanol daun ungu (Gratophyllum pictum (L.)) untuk menurunkan kadar TNF-Î± dan NO Nur Patria Tjahjani; Tri Nur Kristina; Endang Sri Lestari
Pharmaciana Vol 6, No 2 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Ethanol extract of Gratophyllum pictum L (G. pictum L). leaves contain phytochemical compounds such as alkaloids, tannins and flavonoids, which have antibacterial activity against Staphylococcus aureus. This study aims to prove the effectiveness ofÂ ethanol extract ofÂ G. pictum L. to reduce the level of TNF-Î± and NO in Swiss mice infected with S. aureus.The study treated 30 Swiss mice divided into 5 groups, consist of: K (-) normal mice ; K (+) mice were infected with S. aureus ; P1, P2, P3 were infected with S. aureus and given the ethanol extract of G. pictum leaves,75,150, and 300 mg/kgBW/day for 7 days . Mean levels of TNF-Î± of group K(-), K (+), P1, P2, P3 were 300.29; 550.29; 390.34; 517.67; 327.55 pg / mL respectively. One Way ANOVA test showed that there were significant differences between all treatment groups (p = 0.001), LSD Post Hoc test revealed P1 and P3 groups different significantly with K (+) with p= 0.013 and p = 0.001. Mean levels of NO of group K (-), K (+), P1, P2, P3 were 1.32; 3.56; 2.50; 2.64; and 1.56 Î¼M/mL respectively. Kruskal WallisTest showed that the levels of NO not significantly different, although NO levels at 300 mg/kgBW/day similar with to the levels of NO group K(-). Spearman correlation test showed that there were correlations between TNF-Î± and NO with the variation results on each treatment groups. Ethanol extract ofÂ G. pictum leaves at 300 mg/kgBW/day for 7 days is regarded as the effective dose to reduce TNF-Î± and NO levels in Swiss mice infected by S. aureus.

The Effects of Green Betel Leaf (Piper betle) Extract Eye Drops on the Number of Staphylococcus aureus Colonies in Conjunctivitis Wistar Rats Model (Rattus novergicus) Mahira Aisyah Putri Nur; Sudaryanto Sudaryanto; Endang Sri Lestari; Erwin Kresnoadi
Majalah Obat Tradisional Vol 26, No 2 (2021)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.65242

Irrational use of antibiotics can cause resistance to some diseases such as bacterial conjunctivitis caused by Staphylococcus aureus. Previous research said that green betel leaf (Piper betle) contains antimicrobial compounds such as eugenol, cavalry, tannins, saponins, and flavonoids which also have antiseptic power as well as antibiotics. This study aims to prove the decrease in the number of Staphylococcus aureus colonies after giving green betel leaf eye drops (Piper betle) to conjunctivitis Wistar rats (Rattus norvegicus). This research was true experimental with a pretest-posttest control group design. 25 male white Wistar rats as objects were selected by simple random sampling and grouped into 5 groups randomly. The rats were given Staphylococcus aureus, control group K+ was given 0.5% levofloxacin eye drops, and K- was given aquadest as a comparison. Treatment groups P1, P2, and P3 were given different concentrations of betel leaf eye drops. Swab pretest was done 3 days after the rats were inoculated with Staphylococcus aureus and swab post-test was done 5 days after the rats were given green betel leaf eye drops, the calculation of Staphylococcus aureus bacteria colonies used the Total Plate Count (TPC) method. There was a significant decrease (p<0.05) in the number of bacterial colonies for K-, K+, P1, P2, and P3 groups. In addition, there was a significant difference (p<0.05) between K+ with P1 and K+ with P3. There was a decrease in the number of Staphylococcus aureus for all groups of conjunctivitis Wistar rats (Rattus norvegicus)

PERBANDINGAN PEMAKAIAN CEFTRIAXONE TERHADAP INFEKSI LUKA OPERASI PADA PASIEN APENDISITIS AKUT NON KOMPLIKATA YANG DILAKUKAN LAPARATOMI DAN LAPARASKOPI APENDIKTOMI Ilham Farizal; Sigit Adi Prasetyo; Endang Sri Lestari
Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal) Vol 5, No 4 (2016): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Latar belakang : Banyaknya pemberian antibiotik Ceftriaxone pada pasien apendisitis akut non komplikasi yang dilakukan laparaskopi dan laparatomi apendiktomi dikhawatirkan menjadi salah satu penyebab timbulnya infeksi luka operasi (ILO). Ceftriaxone memang memiliki indeks teraupeutik yang tinggi. Namun spektrum antibiotik yang luas dikhawatirkan akan membunuh flora normal. Hal ini dikhawatirkan dapat mengakibatkan resistensi antibiotik dan angka kejadian ILO semakin bertambah.Tujuan : Mengetahui perbedaan pemakaian antibiotik ceftriaxone dan non ceftriaxone terhadap infeksi luka operasi operasi (ILO) pada pasien apendisitis akut non komplikata yang dilakukan laparatomi dan laparaskopi apendiktomi.Metoda : Penelitian dilakukan dengan menggunakan data sekunder rekam medik RSUP Dr.Kariadi tahun 2013-2016. Pengambilan data pasien apendisitis akut non komplikata diambil berdasarkan kelompok yang diberi antibiotik profilaksis ceftriaxone dan non ceftriaxone baik laparoskopi dan laparotomi apendiktomi. Data tersebut diuji dengan pengujian Mann-Whitney non parametrik 2 variabel kategorik tidak berpasangan antara antibiotik ceftriaxone-non ceftriaxone dengan tanda-tanda ILO. Kedua variabel dianalisis berdasarkan kelompok secara keseluruhan, laparoskopi, dan laparotomy. Setelah itu dilihat nilai signifikansinya.Hasil : Jumlah pasien yang dilakukan tindakan laparatomi apendiktomi sebesar 54,8% (17/31). Dari 17 pasien yang dilakukan laparatomi,70,5% diberikan antibiotik ceftriaxone dan 11,7% diberikan antibiotik non ceftriaxone. Sedangkan jumlah pasien yang dilakukan tindakan laparaskopi apendiktomi sebesar 45,2% (14/31). Dari 14 pasien yang dilakukan laparaskopi, 64,2% diberikan antibiotik ceftriaxone dan 35,7% diberian antibiotik non ceftriaxone. Tidak ada perbedaan yang bermakna antara pemberian antibiotik ceftriaxone dan non ceftriaxone terhadap infeksi luka operasi setelah dilakukan laparatomi apendiktomi (p=0,793). Tidak ada perbedaan yang bermakna antara pemberian antibiotik ceftriaxone dan non ceftriaxone terhadap infeksi luka operasi setelah dilakukan laparaskopi apendiktomi (p=0,273).Simpulan : Tidak ada perbedaan yang bermakna antara kejadian infeksi luka operasi terhadap pemberian antibiotik ceftriaxone dan non ceftriaxone baik yang dilakukan laparoskopi apendiktomi maupun laparotomi apendiktomi.

THE EFFECT OF VCO TO THE GROWTH OF CANDIDA ALBICANS ON DENTURE BASIS OF ACRYLIC RESIN Vivi Indah Fatmasari; Rebriarina Hapsari; Nadia Hardini; Endang Sri Lestari
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 9, No 6 (2020): DIPONEGORO MEDICAL JOURNAL (Jurnal Kedokteran Diponegoro)
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dmj.v9i6.29329

Background: Acrylic resin was a material of denture base which frequently used, but it still had a weakness that it was porous, then, it was often the breeding ground of Candida albicans. Virgin coconut oil (VCO) contained fatty acid which was effective to hamper the growth of C.albicans. Objective: This research was to identify the effect of VCO on the growth of C.albicans on acrylic resin denture. Methods: This research was an experimental laboratory research which exerted post-test control group design. The total sample 24 which were divided into 3 groups of treatment and 1 group of control. The treatment covered acrylic plates soaking which have been contaminated with C.albicans suspension into VCO in concentration level 25%, 50%, 75%, and sterile aquades for 8 hours. The acrylic plate was vibrated to knock out C.albicans and was then continued by colony counting on the media of Sabouraud Dextrose Agar (SDA). The statistic test employed Kruskal-Wallis and continued to the Post-Hoc test which employed Mann-Whitney. Findings: The VCO in concentration level 25%, 50%, and 75% affected to inhibit the growth of C.albicans. In the Kruskal-Wallis test, it was found a significant difference between the number of C.albicans on the control group and the VCO in concentration level 25%, 50%, and 75% (p<0,05). The Post-Hoc Mann-Whitney test indicated no significant difference between VCO in concentration level 25% and 50%, 25% and 75%, also 50% and 75% (p>0,05). Conclusion: The VCO in concentration level 25%, 50%, and 75% affected was able to hamper the growth of C.albicans. Further, there was no significant difference in each VCO concentration.

KUALITAS PENGGUNAAN ANTIBIOTIK PADA KASUS OBSTETRI-GINEKOLOGI SEBELUM DAN SESUDAH KAMPANYE PENGGUNAAN ANTIBIOTIK SECARA BIJAK DI RSND Nabila Adzhana; V. Rizke Ciptaningtyas; Winarto Winarto; Endang Sri Lestari
Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal) Vol 8, No 4 (2019): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Latar Belakang: Antibiotik merupakan obat yang berfungsi menghambat pertumbuhan atau membunuh mikroorganisme. Pemakaian antibiotik yang tidak bijak dapat menimbulkan resistensi bakteri terhadap antibiotik. Bakteri yang telah menjadi resisten terhadap antimikroba menjadi permasalahan penting di berbagai pelayanan kesehatan. Dalam rangka mengendalikan resistensi antimikroba, dibutuhkan pengendalian penggunaan antibiotik secara bijak yang sesuai dengan Permenkes RI No.8 tahun 2015 tentang Program Pengendalian Resistensi Antimikroba (PPRA) di Rumah Sakit. Tujuan: Mengetahui dan mengevaluasi kualitas penggunaan antibiotik pada pasien kasus obstetri-ginekologi sebelum dan sesudah kampanye penggunaan antibiotik secara bijak Metode: Penelitian kuasi eksperimental dengan studi pre & post group design dengan menggunakan analisis chi square & Fisher exact test. Penilaian kualitas penggunaan antibiotik dengan menggunakan kategori kualitas Van der Meer & Gyssens, dengan melakukan esktraksi data dari 34 rekam medis pasien kasus obstetri ginekologi Rumah Sakit Nasional Diponegoro pada periode sebelum kampanye, dan 34 rekam medis pasien sesudah kampanye penggunaan antibiotik secara bijak. Hasil: Kualitas penggunaan antibiotik dengan kategori 0(bijak) pada kasus Obstetri-Ginekologi RSND sebelum kampanye sebesar 7,4% dan terjadi penurunan kualitas penggunaan antibiotik pada periode sesudah kampanye menjadi sebesar 7,0%. Penggunaan antibiotik secara tidak bijak(kategori I-V) meningkat dari 92,6%(sebelum kampanye) menjadi sebesar 93,6% sesudah pelaksanaan kampanye penggunaan antibiotik. Penggunaan antibiotik kategori V (tanpa indikasi) mengalami penurunan dari 64,2%(sebelum kampanye) menjadi 61,6% sesudah kampanye penggunaan antibiotik secara bijak. Simpulan: Tidak terdapat peningkatan kualitas penggunan antibiotik yang bijak sebelum dan sesudah kampanye penggunaan antibiotik secara bijak pada kasus obstetri ginekologiKata Kunci: antibiotik profilaksis, kategori Van der Meer & Gyssens, kasus obstetri ginekologi

KUANTITAS PENGGUNAAN ANTIBIOTIK PADA PASIEN BEDAH ORTOPEDI RSUP DR. KARIADI SEMARANG Anangga Haryanto; Agus Priambodo; Endang Sri Lestari
Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal) Vol 5, No 3 (2016): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Background : The development of technology nowadays gives a lot of benefits, however it creates some negative effects such as decreasing physical activity that can cause obesity in the population. Obesity creates some chronic diseasessuch as cancer and heart disease. The composition of body consists of fat mass and fat free mass. Zumba is one of the most popularphysical exercise that can decrease percentage of body fat.Aim : To observe the effect of Zumba exercise on body fat percentage in young females.Method: This is an analytic observasional study with cross sectional design. Research subjects were females aged 20-25 years who experienced Zumba exercise (n=24). The percentage of body fat was measured with Bioelectric Impedance Analysis (Glass Body Analyzer 835) weight scales. Correlation between the period of Zumba exercise and body fat percentage was analyzed using Spearman’s rho correlation test.Results: The body fat percentage in subjects who were conducted Zumba for ≥8 weeks was lower compared to subjects who were conducted Zumba for <8 weeks. The body fat percentage average in subjects who experienced Zumba for<8 weeks is 31,97 ± 5,27 with 25,7 was the lowest score and 42,9 was the highest score, and the average percentageof subjects who experienced Zumba for ≥8 weeks was 27,47±2,96 with 22,6 was the lowest score and 42,9 was the highest. There was a negative correlation with moderate degree of body fat percentage with Zumba exercise (r=-0,421; p=0,04) in Spearman’s rho test.Conclusion : The study found thedifferences between the average of body fat percentage in subjectswhoexperiencedZumbaexercisefor<8 weeks and≥8weeks. This study also showed a negative correlation with moderate degree between body fat percentage and Zumba exercise period.

KUALITAS DAN KUANTITAS PENGGUNAAN ANTIBIOTIK PADA KASUS PENYAKIT DALAM SEBELUM DAN SETELAH PENYULUHAN PPRA DI RUMAH SAKIT NASIONAL DIPONEGORO Nathalia Tiara Mulia Kartika; Endang Sri Lestari; Helmia Farida; V. Rizke Ciptaningtyas
Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal) Vol 8, No 4 (2019): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Latar Belakang: Salah satu penyebab penggunaan antibiotik yang tidak tepat adalah kurangnya pengetahuan dari tenaga medis. Edukasi yang efektif dapat meningkatkan pengetahuan tenaga medis dalam menggunakan antibiotik secara bijak. Edukasi tersebut dapat berupa penyuluhan tentang PPRA (Program Pengendalian Resistensi Antimikroba). Tujuan: Menganalisis kualitas dan kuantitas penggunaan antibiotik pada kasus penyakit dalam sebelum dan setelah penyuluhan PPRA di Rumah Sakit Nasional Diponegoro (RSND). Metode: Penelitian ini menggunakan desain suatu studi intervensi. Sampel adalah 68 rekam medis pasien RSND dengan kasus penyakit dalam yang diterapi antibiotik, masing-masing 34 sampel sebelum dan setelah penyuluhan. Kualitas dan kuantitas penggunaan antibiotik dibandingkan periode sebelum dan setelah penyuluhan. Penilaian kualitas penggunaan antibiotik berdasarkan kriteria van der Meer – Gyssens dengan menggunakan uji Chi-square. Penilaian kuantitas penggunaan antibiotik berdasarkan klasifikasi Anatomical Therapeutic Chemical (ATC) dan satuan Defined Daily Dose/ 100 pasien-hari dengan menggunakan uji independent t test atau uji Mann-Whitney. Hasil: Kualitas penggunaan antibiotik kategori bijak meningkat dari 28,0% menjadi 33,3%, kategori tidak bijak menurun dari 26,0% menjadi 4,8%, dan kategori tanpa indikasi meningkat dari 46,0% menjadi 61,9% setelah penyuluhan (p = 0,022). Defined Daily Dose/ 100 pasien-hari sebelum penyuluhan adalah 103,65 dan setelah penyuluhan adalah 99,63 (p = 0,092). Kesimpulan: Penyuluhan yang diberikan tidak cukup untuk memperbaiki kualitas dan kuantitas penggunaan antibiotik, diperlukan intervensi lain pada faktor pendukung seperti kebijakan rumah sakit, sistem reward and punishment serta pemberian umpan balik.Kata Kunci: Kualitas, kuantitas, penggunaan antibiotik, penyakit dalam, penyuluhan, PPRA

Title

Found 25 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 25 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 25 Documents
Search