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Effect of Temperature Shock on the Triploidization Success of Seurukan Fish (Osteochilus vittatus) Eriani, Kartini; Syahrin, Alfis; Muchlisin, Zainal Abidin
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8680

Seurukan fish (Osteochilus vittatus) has many advantages, besides the fish also has disadvantages which are the slow growth, so the temperature shock of triploidization technique was expected to solve the problem. The objective of the present study was to obtain an effective temperature to increase of triploidization success of seurukan fish (Osteochilus vittatus). The experimental method and completely randomized design model were used in this study. Five levels of temperature shocks at three replicates were tasted: 4? (cold), 6? (cold), 28? (normal), 35? (heat) and 37? (heat). The sperms and eggs were fertilized in the plastic jar then a total 100 of fertilized eggs (zygotes) were taken randomly 3 minutes after fertilization and soaked in respective temperature for 90 seconds, and then incubated in incubation jars at the water temperature of 28-29?. The results showed that the temperature shock gave the significant effect on the hatching and the success of triploidization success (P?0.05), but did not give the significant effect the fertility and survival rates (P>0.05). The triploid fish can be achieved using cold and heat shock, but the higher triploid fish was recorded at 37? was the best temperature recommended for triploidization of Seurukan fish.

Neurogenic Differentiation of Bone Marrow Mesenchymal-Like Stem Cell Induced by Delonix regia Flowers Extract Ichsan, Ichsan; Boediono, Arief; Eriani, Kartini; Suryani, Irma; Azhar, Al; Nursanti, Risa
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.15051

Stem cell technology has great potential in the effort to cure degenerative diseases. This study was done to determine optimum dose of flamboyant (Delonix regia) flower extract to induce proliferation and differentiation of mice (Mus musculus) bone marrow mesenchymal-like stem cell. Bone marrow cells were collected from mice by aspiration. Cells suspension (1 x 106) were poured into petri dishes containing 2 ml of modified Dulbeccos Modified Eagles Media (mDMEM) and incubated overnight at 37 Â°C in a 5% CO2 incubator and microscopically observed. In quadriplicate, MSC were cultured in mDMEM containing D. regia flower extract of 0.0 (control), 0.4, 0.6, 0.8, and 1.0 mg/ml and incubated at 37 Â°C for 9 days. Population doubling time (PDT) and differentiated cell type were microscopically observed using HE staining on day 1 and 10. Data obtained were analyzed by ANOVA and Tukey test. The results showed that the addition of D regia flowers extracts 0.8 and 1.0 mg/ml significantly reduced PDT compared to that of 0.4, 0.6 and control. The extract, at 0.4 and 0.6 mg/ml, were able to induce MSC differentiation into fibroblast-like and nerve-like cells. In conclusion, D. regia flower extracts of 0.6, 0.8 and 1.0 mg/ml were able to stimulate MSC proliferation, but optimum dose for neurogenic differentiation was 0.6 mg/ml. This is the first to show potential of D. regia flower extract as neurogenic differentiatian inducer on mice MSC. These findings can be used as preliminary information for using the extract as cellular differentian inducer in basic and applicative reseach using stem cells.

Cryopreservation of Aceh Swamp Buffalo (Bubalus bubalis) Semen with Combination of Glycerol and Lactose Eriani, Kartini; Sari, Nisa; Rosnizar, Rosnizar; Dasrul, Dasrul; Suhartono, Suhartono; Rizal, Muhammad
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Aceh swamp buffaloes(Bubalus bubalis) are decreasing their population and genetic quality. This study was conducted to determine the influence of lactose and glycerol cryoprotectants on spermatozoa of Aceh swamp buffaloesafterthawing.This study used completelya factorial randomized design with nine treatments, and five replications.Fresh semen of the Aceh swamp buffalowere diluted by using a combination extender lactose cryoprotectants 0 mM (L0), 60 mM (L60), 120 mM(L120) and glycerol 3% (G3), 5% (G5), 7% (G7) with the equilibration of 4 hours.The results showed that the combination of cryoprotectants L120G7 influenced significantly (P < 0.05) on the quality of spermatozoa of the Aceh swamp buffalo(B. bubalis)after thawing.The percentage of sperm motility L120G7 (42.60 1.14);viability L120G7 (55.00 0.71);acrosome integrity L120G7 (52.00 0.71); and plasma membrane integrity L120G7(53.20 1.48). The combination of lactose cryoprotectants 120 mM (L120) and glycerol 7% (G7) was the best combination to maintain the quality of spermatozoa of swamp buffalo. This finding could be used to define a policy for the spermatozoa storage of Aceh swamp buffalo to artificial insemination (AI).

Pengaruh pemberian Ekstrak Etanol Akar Anting-Anting (Acalypha indica L.) terhadap kualitas Spermatozoa Mencit Yasmin, Cut; Eriani, Kartini; Sari, Widya
Jurnal Kedokteran YARSI Vol 18, No 1 (2010): JANUARI - APRIL 2010
Publisher : Lembaga Penelitian Universitas YARSI

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol akar anting-anting (Acalypha indica L.) terhadap kualitas spermatozoa mencit. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) terdiri atas empat perlakuan dengan lima ulangan. Perlakuan terdiri atas pemberian ekstrak etanol akar anting-anting dengan dosis: 0 mg/kg bb (P0), 150 mg/kg bb (P1), 300 mg/kg bb (P2), dan 600 mg/kg bb (P3) yang diberikan sekali sehari selama 7 hari. Parameter kualitas spermatozoa adalah motilitas spermatozoa, keutuhan membran plasma, spermatozoa hidup, dan abnormalitas spermatozoa dari 200 spermatozoa. Data hasil penelitian dianalisis dengan analisis varian dan dilanjutkan dengan uji Jarak Berganda Duncan. Hasil penelitian menunjukkan ekstrak etanol akar anting-anting berpengaruh nyata dalam meningkatkan motilitas spermatozoa, jumlah spermatozoa dengan membran plasma utuh, dan jumlah spermatozoa hidup. Pemberian ekstrak etanol akar anting-anting dengan dosis 300 mg/kg bb dan 600 mg/kg bb merupakan dosis yang dapat meningkatkan libido, sedangkan dosis untuk meningkatkan kualitas spermatozoa dosis adalah 600 mg/kg bb.

Pengaruh Medium Kultur Bebas Serum terhadap Perkembangan Preimplantasi Embrio Mencit in vitro Eriani, Kartini; Sunarti, Sunarti; Nasir, M.; Djuwita, I.
Jurnal Kedokteran YARSI Vol 16, No 1 (2008): JANUARI - APRIL 2008
Publisher : Lembaga Penelitian Universitas YARSI

The use of serum in a culture medium is a common practice in the study of mouse embryo development in vitro. However, the role of unknown factors in serum influencing the embryo development has been difficult to determine. The aim of this study was to investigate the effect of amino acid in serum free medium on in vitro development of mouse embryo. This study was conducted in the following ways: (1) medium M16 supplemented with 0.3% BSA, (2) medium M16 supplemented with 2% v/v MEM, and (3) medium M16 supplemented with 2% v/v MEM and 0.1 mmol of glutamine. The embryos were collected in 3 stage of development: zygote (day-1), morula (day-3) and compacted morola (day-4). The results indicated that supplementation of amino acid into M16 culture medium could replace the role of BSA aims at supporting the developmental potency of mouse embryos in vitro.

Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation Dwitya, Yonadiah; Eriani, Kartini; Saputra, Hendra; Al-Azhar, Al-Azhar; Rizal, Muhammad
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Cryopreservation process could affect spermatozoa quality during from reactive oxygen species (ROS) produced in cellular metabolism and the environment. Spermatozoa damage caused by ROS during cryopreservation can be reduced with the addition of natural antioxidant which commonly found in fruits like date palm. This research was done to investigate the influence of date extract on semen quality after cryopreservation. This experimental study used a completely randomized design with 4 treatments and 6 replications. Semen collected from two aceh cattle bulls was diluted in tris egg yolk extender contained different concentrations (v/v) of date extract: 0% (P0, control), 0.75% (P1), 1% (P2), and 1.25% (P3) before cryopreserved at -196 ºC for 7 days. Semen quality prior to and after cryopreservation as well as sperm DNA integrity were determined by standard microscopic and laddering methods, respectively. The results showed that the addition of 1% date extract could maintain viability (68.67%), plasma membrane integrity (62.33%), and abnormality (18.58%) of aceh cattle spermatozoa, but unable to maintain its motility above 40%. There was no DNA fragmentation observed in both treated and fresh semen. This is the first study investigates the influence of supplementation of date palm extract on preserved aceh cattle spermatozoa diluted in egg yolk tris based extender.

Utilization of Oocytes Collected from Preserved Ovarian for In Vitro Production of Cat Embryos Eriani, Kartini; Boediono, Arief; Sumarsono, Sony Heru; Azhar, Al
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Preservation of ovarian tissue from severely injured or dead valuable animals has the potential to preserve female germ cells of animals. The ability to mature and fertilize of oocytes from preserved ovary of endangered species will allow us to sustain genetic and global biodiversities. The aims of this study were to investigate the viability of oocytes collected from the preserved ovary and its potential utilization for the production of cat embryos followed by in vitro maturation and fertilization. Ovary was preserved immediately in phosphate buffer saline (PBS) at 4 °C for 24 or 48 hours. The quality and viability of oocytes after the maturation process were identified microscopically using aceto-orcein staining. Biological function of the oocytes was evaluated by using in vitro culture technique for the maturation and fertilization rate in CR1aa medium culture. The results showed that the percentage of oocytes collected from preserved ovary for 24 and 48 hours that remained at the stage of metaphase-II were 29.4% and 21.9% respectively. Fertilization rates produced in the IVF using oocytes collected from ovary preserved for 24 or 48 hours were significantly lower (30%) than that of unpreserved control (36.7%). In conclusion, female germ cells of cat ovary preserved at 4 °C in PBS for 2 days were still viable for in vitro fertilization and thus can be utilized for in vitro production of cat embryos. Information obtained can be used as a basis of knowledge of using a combination of physiological reagent and cold-based preservation technique in modern reproductive technology for animals.

The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation Mahfudhah, Raudhah; Eriani, Kartini; Muchlisin, Zainal Abidin
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

The cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent. This study aimed to examine the effect of the addition of different doses of glutathione in cryopreservation of Aceh Local catfish (Clarias gariepinus) spermatozoa after freezing. The method used was a completely randomized design (CRD) with four treatments and four replications. Fresh semen was diluted in Ringer, 15% DMSO, and 20% Fetal Bovine Serum (FBS) and then added with glutathione antioxidants of 0.0 mgL-1, 0.5 mgL-1, 1.0 mgL-1, and 2.0 mgL-1. The parameters observed in this study were motility, integrity of the plasma membrane, fertility, and DNA integrity. The results showed that the concentration of glutathione had no effect on motility, integrity ofthe plasma membrane, or fertility, but had an effect on DNA integrity. The highest percentage of motility and plasma membrane integrity respectively was 40.50% (P3) and 70.87% (P2). Furthermore, the assessment of DNA integrity showed that there was no DNA fragmentation both treatments and fresh spermatozoa. This research is the first study regarding glutathione supplementation in cryo-diluent of Aceh Local catfish spermatozoa. Finally, the results obtained can provide information about the exact concentration of glutathione in the extender on the quality of spermatozoa of Aceh Local catfish (C. gariepinus) after the cryopreservation process. These results can also increase the success of fertility be used by the seed hall unit and the aquaculture industry to increase the productivity and supply high quality seeds.

EFEK EKSTRAK ETANOL AKAR ANTING- ANTING (ACALYPHA INDICA) TERHADAP LIBIDO MENCIT Yasmin, Cut; Eriani, Kartini; Sari, Widya
Jurnal Kedokteran YARSI Vol 21, No 1 (2013): JANUARI - APRIL 2013
Publisher : Lembaga Penelitian Universitas YARSI

Penelitian ini bertujuan mengevaluasi efek pemberian ekstrak etanol akar anting-anting (Acalypha indica) terhadap libido mencit. Penelitian ini meng-gunakan Rancangan Acak Lengkap, terdiri atas empat perlakuan dengan lima ulangan. Perlakuan terdiri atas pemberian ekstrak etanol akar anting-anting dengan dosis: 0, 150, 300, dan 600 mg/kg bb yang diberikan sekali sehari selama 7 hari. Parameter libido yang diamati adalah mounting latency, intromission latency, dan jumlah orgasme. Data hasil penelitian dianalisis dengan analisis varian dan dilanjutkan dengan uji Jarak Berganda Duncan. Hasil penelitian menunjukkan ekstrak etanol akar anting-anting berpengaruh nyata dalam penyingkatan bermulanya mounting dan intromission, serta meningkatkan jumlah orgasme. Pemberian ekstrak etanol akar anting-anting dengan dosis 300 dan 600 mg/kg bb merupakan dosis yang dapat meningkatkan libido.This research was aimed to evaluate the effect of anting-anting (Acalypha indica) root ethanol extract on sexual arousal of mice. The experimental method with completely randomized design was applied, that consisted of four treatments and five repetition. The treatments were 0, 150, 300, dan 600 mg/kg bw anting-anting root ethanol extract given once a day for 7 days. Observed parameter were mounting latency, intromission latency, and the number of orgasm. The data was analyzed by analysis of variance and continued by Duncan?s multiple range test. The result showed that anting-anting root ethanol extract was significantly difference to shorten the starting of mounting, intromission, and increasing the number of orgasm. The application of anting-anting ethanol extract with dose of 300 and 600 mg/kg bw was able to increase sexual arousal.

Alkaline Phosphatase Expression From Mice Mesenchymal Stem Cells Induced By Flamboyant Flower (Delonix regia) Extract Eriani, Kartini; Anggraini, Deby; Bintoro, Yudha; Ichsan, Ichsan; Azhar, Al; Mariya, Silmi
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25433

Flamboyant flower (Delonix regia) extract can increase proliferation and differentiation rates of mesenchymal stem cells (MSCs) into specific cells such as bone, nerve, and fibroblast cells. The extract possess metabolic compounds that may act as antibiotics, anti-inflammatory, antimicrobial, diuretic, anthelmentic, astringent, leucorrhoea, and potentially increase the body's metabolism normally. This study aimed to investigate expression level of alkaline phosphatase (ALP) by mice MSCs treated with flamboyant flower extract in vitro. Here, mice bone marrow cell cultures were treated with flamboyant flower extracts of 0.6 mg/ml (P1), 0.7 mg/ml (P2), 0.8 mg/ml (P3), and 0.9 mg/ml (P4). Untreated cell culture was used as negative control (P0). Expression of ALP gene was measured by RT-qPCR method. The results showed that mice mesenchymal stem cell could differentiate into bone, nerve, and fibroblast cells. The addition of flamboyant flower extract ranged from 0.6-0.9 mg/ml significantly (p<0.05) influenced the expression of ALP by differentiating MSCs. The highest expression was found at the stem cells treated with flamboyant flower extract of 0.8 mg/ml, 0.13 times compared with control. In conclusion, flamboyant flower extracts treatment might increase the expression of ALP in differentiating MSCs. Â This information can be used as a basis for finding an appropriate biomarkers for tracking the differentiation and profileration of tissue originated MSCs induced by extracts of medicinal plants.

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