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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Hortikultura Indonesia Jurnal Ilmiah Farmasi Bioplante Biospecies Jurnal Natur Indonesia Biogenesis: Jurnal Ilmiah Biologi JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Media Pertanian Riau Journal of Empowerment Jurnal Agroecotania : Publikasi Nasional Ilmu Budidaya Pertanian Menara Perkebunan AGROSCRIPT Journal of Applied Agricultural Sciences Bubungan Tinggi: Jurnal Pengabdian Masyarakat Pelita Masyarakat Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Jurnal Karya Abdi Masyarakat Jurnal Ilmiah Ilmu Terapan Universitas Jambi

Lizawati Lizawati

Fakultas Pertanian, Universitas Jambi

Author-ID : 2677149

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Medicine & Pharmacology Physics Public Health Social Sciences Other

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Proliferasi Kalus dari Eksplan Hipokotil dan Kotiledon Tanaman Jarak Pagar (Jatropha curcas L.) pada Pemberian 2,4-D Zulkarnain, Zulkarnain; Lizawati, Lizawati
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The aim of this study was to develop an efficient method for the induction of embryogenic callus formation for in vitro propagation ofjatropha. Plant materials used were 30-days old in vitro seedlings, cut into hypocotyl and cotyledon (lower, middle and upper) sections.Medium used was MS composition supplemented with vitamins, 3% sucrose, 0.7% agar at pH 5.8 ± 1, and 2,4-D (0, 1, 2, 3, 4 dan5 mg l-1). Cultures were kept at temperature of 25 ± 1 0C with 50 μmol m-2 s-1 light intensity and 16-h photoperiod. The results indicated thatthe rate of callus formation depended on the source of explant, the application of 2,4-D, and the interaction of both. The fastest callusproliferation (2.33 days following initiation) was obtained on cotyledon explants cultured on medium without 2,4-D. The explant sourcesand 2,4-D concentrations also showed significant effect on the percentage of explant forming callus. The most callus formation (88.33%)was obtained on middle cotyledon cultured on 3 mg l-1 2,4-D, whereas the fewest (6.84%) was found on upper cotyledon cultured on mediumwithout 2,4-D. The colour of callus was dominated by white, light yellow, cream and brown with mostly compact structure, particularly onhypocotyl cultured on medium without 2,4-D. The texture of callus formed on hypocotyl treated with up to 4 mg l -1 2,4-D was dominatedby coarse appearance. In contrast, majority of callus proliferated on hypocotyl treated with 5 mg l -1 2,4-D or cotyledon treated with orwithout 2,4-D produced callus with smooth texture %.

Pertumbuhan Bibit Tanaman Manggis (Garcinia mangostana L.) Setelah Inokulasi dengan Berbagai Galur Agrobacterium rhizogenes1 , Lizawati; Roedhy Poerwanto; , Sobir; Iman Rusmana; Tri Muji Ermayanti
Indonesian Journal of Agronomy Vol. 35 No. 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Growth of mangosteen essentially depends on its root system. Therefore, it needs technology to obtain stringer mangosteen root system. The use of Agrobacterium rhizogenes bacterium is an alternative. The objectives of this experiment were : 1) to find the effective strain of A. rhizogenes bacterium for inoculation of mangosteen seedling root, 2) to find the best inoculation method for inducing mangosteen seedling root. The materials used in this experiment were ; mangosteen fruit and A. rhizogenes collection from Puslit Biotechnology LIPI Cibinong-Bogor. The experiment was arranged in completely randomized design with two factorial treatments. The first factor : 11 strains A. rhizogenes (ATCC-15834, ATCC-8196, R-1000, 07-20001, A4, A4-J, 509, 510, 511, MAFF 01-1724, and control), the second factor : 2 inoculation methods (cutting and dipping). The results showed that A. rhizogenes of ATCC-15834, 509, 07-20001, A4, and R-1000 increased stem diameter, plant height, leaf number, lateral and tertiary root number, better than ATCC-8196, MAFF 01-1724, 510, 511, A4-J, and control. Cutting root method of inoculation resulted in higher live plant percentage compared to dipping root method. Key words : Agrobacterium rhizogenes, Garcinia mangostana, inoculation

Induksi dan Multiplikasi Tunas Jarak Pagar (Jatropha curcas L.) Secara In Vitro , Lizawati; Trias Novita; Ragapadmi Purnamaningsih
Indonesian Journal of Agronomy Vol. 37 No. 1 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The conventional propagation of physic nut (Jatropha curcas) is difficult, because it requires a high number of mother plant, which is very limited. In vitro culture is an alternative technique to conventional one to solve the problem. An experiment was done to obtain the best in vitro culture media for shoot induction and multiplication. This research was separated into two steps, (1) in vitro induction of explant growth, and (2) in vitro shoot multiplication. Results showed that medium of WPM + 2.0 ppm BAP induced shoot and leaf better than the control. The highest number of leaf axillary's multiplication was obtained from the medium WPM + 2.0 ppm BAP + 0.1 ppm NAA. Various medium formulations for the induction and multiplication of shoots resulted in highly leaf fall. The use of DKW + 2.0 ppm BAP + 0.4 ppm TDZ + 3.0 ppm AgNO3 medium has effectively induced shoot multiplication and reduction of dehydrated leaf. Meanwhile, the used of DKW medium supplemented with 5 ppm kinetin resulted in the best shoot elongation. Key words : Induction, in vitro, Jatropha curcas, shoot, multiplication

Callus Proliferation from Immature Leaf Explants of Durian (Durio zibethinus Murr. cv. Selat) with the Addition of Picloram and BAP Zulkarnain ,; , Lizawati
Jurnal Hortikultura Indonesia Vol. 4 No. 3 (2013): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTThis study was aimed to obtain an appropriate medium composition with various combinations of Picloram + BAP for the proliferation of embryogenic callus from immature leaf explants of durian. The experiment was carried out at the Plant Biotechnology Laboratory, Faculty of Agriculture , the University of Jambi from January through to November 2012. Five levels of Picloram (1.0, 2.0, 3.0, 4.0, 5.0 mg L-1) in combination with three levels of BAP (0, 0.5, 1.5 mg L-1) were tested. Therefore, there were 15 treatment combinations with 4 replicates resulting in 60 experimental unit. Each unit consisted of 4 culture flasks containing one immature leaf explant. Cultures were kept in culture room with 16 h photoperiod and 1000 lux light intensity. The results showed that: 1) callus proliferation on immature leaf explants of durian cv. Selat was dependent upon the level of Picloram + BAP added to culture medium, 2) the addition of 3.0 - 5.0 mg L-1Picloram without BAP was found to be effective in promoting callus proliferation on the majority of cultured explants, 3) all regenerated callus showed similar characteristics, but embryogenic properties was not seen yet, and 4) the application of tissue culture technique in the propagation of durian cv. Selat needs further comprehensive investigation, particularly on factors directly affecting culture development and inducing somatic embryogenesis.Key words: tissue culture, in vitro culture, micropropagation, plant hormones, auxin, cytokinin, fruit crops. ABSTRAKPenelitian ini bertujuan untuk mendapatkan komposisi media yang tepat dari kombinasi Picloram + BAP untuk proliferasi kalus embriogenik dari eksplan daun dewasa durian. Penelitian dilakukan di Laboratorium Bioteknologi Tanaman, Fakultas Pertanian, Universitas Jambi dari Januari hingga November 2012. Perlakuan kombinasi media zat pengatur tumbuh adalah lima taraf Picloram (1.0, 2.0, 3.0, 4.0, 5.0 mg L-1) dengan kombinasi tiga taraf perlakuan BAP (0, 0.5, 1.5 mg L-1). Terdapat 15 kombinasi media perlakuan dengan 4 ulangan, sehingga terdapat 60 kombinasi satuan percobaan. Setiap unit percobaan terdapat 4 botol kultur dengan satu eksplan daun. Kultur disimpan di ruang kultur selama 16 jam penyinaran dan intensitas cahaya 1000 lux. Hasil penelitian menunjukkan bahwa: 1) Proliferasi kalus dari eksplan daun muda buah durian tergantung pada taraf kombinasi picloram + BAP yang ditambahkan ke media kultur; 2) Penambahan 3.0-5.0 mg L-1Picloram tanpa BAP memberikan hasil yang efektif untuk menginduksi proliferasi kalus pada sebagian besar eksplan; 3) Regenerasi kalus menunjukkan karakteristik serupa, tetapi embriogenik kalus tidak muncul, dan 4) Perbanyakan eksplan daun durian dengan tehnik kultur jaringan membutuhkan penelitian lebih lanjut, terutama pada faktor yang berpengaruh langsung pada induksi embriogenesis somatik.Kata kunci: auksin, buah, kultur jaringan, kultur in vitro, mikropropagasi, sitokinin, zat pengaturtumbuh,

Application of Indigenous AMF from ex-coal Mining Soil Combined with Phosphorus Fertilizers to Improved Oil Palm Seedling Growth (Elaeis guineensis Jacq.) Made Deviani Duaja; Elis Kartika; Lizawati Lizawati
Biogenesis: Jurnal Ilmiah Biologi Vol 7 No 1 (2019)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v7i1.5990

Opencast mining caused heavy deforestation and barren land in Jambi. This ex-mining land must be reclaimed by planting crops based on biofertilizers containing mycorrhizae, this is a potential alternative that provides benefit both agronomy plant and ecosystem specially replanting with oil palm. Those are the technology for reclamation ex-coal mining soil. This research aim is to examine the effect of four combinations indigenous isolate of Arbuscular Mycorrhizae Fungi (AMF), which is originated from ex-coal mining soil that combined with Phosphorous fertilizers to minimize utilizing P fertilizer at oil palm pre-nursery, to improve soil fertility, and seedling growth. Hence, this research was arranged in factorial experiment with 2 factors, using a complete randomized design with three replications. The first factor is five different mycorrhizae isolates, representing a broad range of endomycorrhizae fungi, there are: without indigenous AMF, isolate of Glomus sp. 3, isolates of Glomus sp. 6, isolates of Glomus sp. 15 and isolates of Glomus sp. 16. The second factor is P fertilizer: control (without P fertilizers), fertilizer dosage P 25%, 50%, 75%, and 100%. Variables measured are shoot height, number of leaves per plant, total leaf area per plant, and plant stem diameter. The data were analyzed by analysis of variance to see whether there was an interaction between the MVA isolate and the P fertilizer dosage. The result showed a significant effect on palm oil seedling growth, shoot height, number of leaves per plant, total leaf area per plant but there is no interaction on the stem diameter. Plants that are inoculated with mycorrhizae have greater P content in leaves than those not inoculated. The types of isolated Glomus sp.3 and dosage P 75% of recommended dosage, give the best seedling growth. This research concluded that inoculation with AMF could minimize P fertilizers doses.

In Vitro Callus Development on Immature Leaf Explants of Liberica Coffee (Coffea liberica L. cv. Liberika Tungkal Komposit) by the Application of 2.4-D and BAP Lizawati Lizawati; Zulkarnain Zulkarnain; Neliyati Neliyati
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.11769

Conventional vegetative propagation is the process in which new plants are grown from a variety of sources, seeds, cuttings and other parts of the plants. Therefore, the conventional vegetative propagation of liberica coffee by cutting or grafting of stems is hampered by the limited number of stem or branches, which can be used as propagating materials. In addition, the tissue culture technique is another method used to propagate liberica coffee. This study aims to investigate an efficient protocol for embryogenic callus development from leaf explants of Coffea liberica cv. Liberika Tungkal Komposit. The explants used are immature leaves of fully opened liberica coffee. The medium used was Murashige and Skoog (MS) composition supplemented with vitamins, 3% sucrose and solidified with 0.7% agar, and the medium pH was adjusted to 5.8 ± 0.1. The experiment was arranged in a factorial randomized block design, and the first factor was 2.4-D (2.0, 3.0, 4.0, and 5.0 ppm) and then the second factor was BAP (0.0, 0.5, and 1.0 ppm). The results showed that the application of 2.4-D and BAP significantly improved the distribution of callus proliferation on cultured explants. The use of 2.0 ppm 2.4-D + 1.0 ppm BAP resulted in the fastest callus proliferation (19 days after culture initiation). In general, the application of different levels of 2.4-D and BAP successfully induced friable with nodular morphology callus on young leaf explant of liberica coffee. It is believe that the callus has embryogenic capacity and will undergo embryogenesis when transferred to a suitable medium composition

ISOLASI, IDENTIFIKASI DAN PEMURNIAN CENDAWAN MIKORIZA ARBUSKULAR (CMA) DARI TANAH BEKAS TAMBANG BATU BARA (Isolation, Identification and Purification of Arbuscular Mycorrhiza Fungi (AMF) from Coal Post Mining Soil) Elis Kartika; . Lizawati; . Hamzah
Bioplantae Vol. 1 No. 4 (2012): Bioplantae
Publisher : Bioplantae

Show Abstract | Download Original | Original Source | Check in Google Scholar

Land of coal post-mining is the critical area that generally can not becultivated due to very low levels of fertility of the land, so this landbecomes slighted. One of the alternatives to overcome this problem isthrough inoculation with Arbuscular Mycorrhiza Fungi (AMF).Indigenous AMF (from coal post-mining location) is more potential AMFdeveloping in that area. Therefore, isolation, identification and purificationsteps of AMF spores are required. The objective of this study was toisolate, identify, and purify of arbuscular mycorrhiza fungi from coal postmining area. The study had identified that at this soil was found 3 AMFgenuses, i.e. Glomus, Acaulospora, and Gigaspora. On coal post-miningsoil was found 20 strains of AMF (13 strains of Glomus, 3 strains ofAcaulospora, and 1 strain of Gigaspora). In this soil was dominated byGlomus. Strain’s AMF that was successful isolated from single sporeculture was 4 strains i.e. Glomus sp-3, Glomus sp-6, Glomus sp-15, danGlomus sp-16.

PROLIFERASI KALUS DAN EMBRIOGENESIS SOMATIK JARAK PAGAR (JATROPHA CURCAS L.) DENGAN BERBAGAI KOMBINASI ZPT DAN ASAM AMINO (Callii Proliferation and Somatic Embryogenesis of Physic Nut (Jatropha curcas L.) Various Combination with PGR’s and Amino Acids) . Lizawati
Bioplantae Vol. 1 No. 4 (2012): Bioplantae
Publisher : Bioplantae

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The aim of this study was to reveal a combination of plant growthregulators (PGR’s) and amino acids best callii proliferation and somaticembryogenesis physic nut. The experiment was arranged in completelyrandomized design with combination of plant growth regulators (PGR’s)and amino acids, i.e. 1 ppm TDZ + 1 ppm 2,4-D + 100 ppm glutamine;2 ppm BAP + 1 ppm 2,4 - D + 100 ppm glutamine; 1 ppm TDZ + 1 ppm2,4-D + 100 ppm CH (Casein hydrolyzate); 2 ppm BAP + 1 ppm 2,4-D+ 100 ppm CH (casein hydrolyzate); 1 ppm TDZ + 1 ppm 2,4-D; 2 ppmBAP + 1 ppm 2,4-D; Mannitol 3%; proline 5,75 ppm; proline 2.875ppm. The parameters observed were callii weight, callii diameter, calliicharacteristics (colour and structure) and the number of embryogeniccallii. The result showed that some treatment combinations of plantgrowth regulators and amino acids can increase the callii weight andcallii diameter. The color of celli was dominated by green and creamwith mostly compact structure. Meanwhile, celli friable structure as intreatment 1 ppm TDZ + 1 ppm 2,4-D; proline 5.75 ppm and 2.875 ppm.Key words : casein hydrolyzate, BAP, glutamine, proline, TDZ

INDUKSI KALUS EKSPLAN DAUN DURIAN (Durio zibethinus Murr. cv. Selat Jambi) PADA BEBERAPA KOMBINASI 2,4-D DAN BAP (Callus Induction Explants Leaf Durian (Durio zibethinus Murr. cv. Selat Jambi) With 2,4-D And Bap Combination) . Lizawati; . Neliyati; Retna Desfira
Bioplantae Vol. 1 No. 1 (2012): Bioplantae
Publisher : Bioplantae

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The aim of this study was to obtain the best combination of 2,4-D and BAP in inducing callus from leaf explants durian cv. Selat Jambi. Experiment was arranged in completely randomized design with a growth regulators combination of treatment 2,4-D (1, 2, 3, 4, 5, 6, 7 ppm) and BAP (0,0 ; 0.5 ppm). Each treatment consisted of 10 bottles each culture bottle were planted on explant culture. Explants were cultured on induction medium for 2 month. The parameter time of calli initiation were observed every day. Meanwhile the percentage of explant forming callus, callus structure and color of callus was observed at the end of the study. The results showed that, granting some combination of growth regulators 2,4-D and BAP are given to the culture medium was able to stimulate the formation of callus on leaf explants young durian CV. Selat Jambi. Time of the fastest callus initiation (8 days after cultur) was obtained on medium 4 ppm 2,4-D + 0.5 ppm BAP. In contrast, the highest percentage of explants forming callus was obtained on treatment of 5 ppm 2,4-D (30%).Key words: Exsplants, Durio zibethinus, callus, BAP, 2,4-D

INDUKSI KALUS EMBRIOGENIK DARI EKSPLAN TUNAS APIKAL TANAMAN JARAK PAGAR (Jatropha curcas L.) DENGAN PENGGUNAAN 2,4 D DAN TDZ Lizawati .
Bioplantae Vol. 1 No. 2 (2012): Bioplantae
Publisher : Bioplantae

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This studyaims at determine the interactionbetween the different level of 2.4-D andTDZ concentrations, to induction theembryogeniccallus of physic nut. This Research used completely randomized design with factorial treatment pattern, which consists oftwofactors :concentration of2,4 –D, which consists of 5levels : 0.0, 2.5, 5.0, 7.5, 10.0 ppm, and the concentration ofTDZconsists of 5levels: 0.0, 0.5, 1.0, 1.5, 2.0 ppm. The results showes that there are interactions between 2,4-D andTDZ to formingcallus ofthe apicalbudexplants of physic nut.The combination of5.0 ppm2,4-D + 1.0ppmTDZand7.5ppm2,4-D + 1.5ppmTDZproducedthe time appearing of callusfastestcompared toother treatments. The highestpercentage ofcallus formation occuredin thetreatment of2.5ppm2,4-D + 1.0ppmTDZand7.5ppm2,4-D + 1.5ppmTDZ. Whiteyellow color, shinyand friable are characteristicof embryogenic callusproduced on thetreatment of0.0 ppmand 2.5ppm2,4-D with the addition ofTDZata concentration of 0.5ppm, 1.0 ppm, 1.5 ppmand 2.0ppm. Key words : embryogenic, callus, TDZ

Co-Authors . Hamzah Addion Nizori Astra Migandi Budiyati Ichwan Buhaira Buhaira Dedy Antony Devi Safitri Elis Kartika Eliyanti Eliyanti Elly Indra Swari FITRY TAFZI Gusniwati , H ASWIDINNOOR Hajar Setyaji Hamzah Hamzah I BOERHENDY Iman Rusmana Indra Lasmana Tarigan Indra Lasmana Tarigan Johannes Made Deviani Duaja Madyawati Latief Madyawati Latief Muhaimin Muhammad Zuhdi N Toruan-Mathius NELIYATI, Neliyati Nelson Nerty Soverda Olya Aprinda RAGAPADMI PURNAMANINGSIH Rajjitha Handayani Ratih Dyah Puspitasari Rayhan Amadius Weihan Retna Desfira ROEDHY POERWANTO Santi Sandra Sobir Sobir Sutrisno Tri Muji Ermayanti Trias Novita Weni Wilia Wilyus Yulia Alia Yusnaidar Yusnaidar Zul Fahri Gani Zulkarnain , ZULKARNAIN ZULKARNAIN Zulkarnain Zulkarnain Zulkarnain Zulkarnain

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