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All Journal Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Journal of Tropical Biodiversity and Biotechnology
Donan Satria Yudha
Fakultas Biologi, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology

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Journal : Biogenesis: Jurnal Ilmiah Biologi

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Monitoring Anurans Diversity along Code River, Province of Daerah Istimewa Yogyakarta, Indonesia Donan Satria Yudha; Wulan Rahmani Akmal; Rury Eprilurahman
Biogenesis: Jurnal Ilmiah Biologi Vol 7 No 2 (2019)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v7i2.10174

Abstract

The research about frogs and toads diversity in the Code River, Province of Daerah Istimewa Yogyakarta (DIY) had been done in 2012 (Yudha et al., 2013). Now, after five years passed, we conduct monitoring activities for updating its diversity and distribution. After five years, we consider that there are many changes along the river that influenced habitat condition for frogs and toads which live along and near the river. Some of the changes are unused land became human settlement or paddy fields and wasteland. The purpose of this research is to monitor the diversity of frogs and toads after five years along Code River. The method used was acoustic and visual encounter survey (VES). We have done total of 10 days of sampling. Each day we did two times sampling, during the day and night. Results show that the species number of frogs and toads found in 2012 were slightly higher compare to 2017. The frogs and toads on upstream part were diverse in 2017 compare to 2012 due to natural restoration of riparian vegetation occur on upstream. The frogs and toads on midstream part were less diverse in 2017 compared to 2012 due to increasing human activities near the river and less riparian vegetation. The frogs and toads on downstream part were also less diverse in 2017 compared to 2012 due to increasing human activities near the river and more household waste stuck on riparian vegetation. There was variation of anurans diversity after five years due to changing on physical condition of the river and human activities near the river.
Comparising DNA extraction from environmental DNA samples to reveal the diversity of freshwater metazoans Donan Satria Yudha; Dwi Sendi Priyono; Rahma Izzati; Aulia Sigit Ardianto; Ananto Puradi; Nainggolan Nainggolan
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.24390

Abstract

Environmental DNA (eDNA) monitoring has gained popularity in the last decade as one of the most sensitive and cost-effective monitoring methods. However, information regarding the type of DNA extraction used still needs to be studied, especially for metazoan in fresh water samples. This parameter is also critical for a project's experimental design. This study aims to compare the effectiveness of two extraction kits between DNeasy® Blood & Tissue Kit (Qiagen) silica column-based and ZymoBIOMICS 96 MagBead DNA Kit (Zymo Research) magnetic bead-based. The quantity of DNA extracts was measured using a spectrophotometer at 260/280 nm. Following that, we continued the metazoa PCR procedure. Qiagen has higher mean value of DNA concentration (88.48 ng/μl) than Zymo (20.89 ng/μl). For DNA purity, Zymo has higher mean value of DNA purity (1.84) than the Qiagen (1.59). However, both kits were equally successful in amplifying universal metazoan primers. We recommend that the use of these types of kits appears to be the least important consideration. Other important factors that may have a major impact on DNA extraction such as water volume, membrane type, sampling strategy need to be investigated in freshwater samples.
Co-Authors Abida, Farahsani Umi Alfonsus Toribio Eko Saputro AMIR HAMIDY Ananto Puradi Ananto Puradi Nainggolan Asmaa’Anugerah Tarekat Aulia Sigit Ardianto Christian Manggala Yudha Pratama Didit Hadi Barianto Dwi Sendi Priyono Elika Boscha Fathiya, Raden Firly Fidelis Triki Sadewa Aritona Haekal, Muhammad Hanan Asyrofi Hastin Ambar Asti Herofi Azhar Imam Sujadi Iman Akbar Muhtianda Kusumardiastuti Kusumardiastuti M Zulfiqar Meizar Pratama Muhammad Ageng Prabowo Muhammad Faisal Alawi Nainggolan Nainggolan Nopitasari, Sri Nugraha, Rizki Nurrochmah Wisudhaningrum Parama Putra, Krisogonus Yudha Puji Lestari Rahma Izzati Rury Eprilurahman Rury Erilurahman Rusyad Adi Suriyanto Setiawan Silva Pambudi Siti Markhamah Suranto, Rosita Dwi Putri Tobing, Vega Felicia Tri Trijoko Trijoko Trijoko Trijoko Trijoko Wajudi Wajudi Widodo Widodo Wulan Rahmani Akmal