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All Journal Biomedical Engineering The Indonesian Biomedical Journal
Mochammad Amin Amin
Institute Of Tropical Disease, Airlangga University Surabaya, East Java, Indonesia
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

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Journal : Biomedical Engineering

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Detection of Hepatitis C Virus RNA in Blood Donors with Nested PCR Technique Supiana Dian Nurtjahyani; Mochammad Amin Amin; Retno Handajani Handajani
Biomedical Engineering Vol 2, No 2 (2016): Biomedical Engineering
Publisher : Biomedical Engineering

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Abstract

Objective: This study was conducted to detect hepatitis C virus (HCV) RNA among blood donors in Tuban, East Java, Indonesia,  using nested Polymerase Chain Reaction (PCR) technique.Material and methods: Seven blood donors  with positives anti-HCV were obtained from 500 blood donors from Red Cross Blood Donor Center (Palang Merah Indonesia = PMI) Tuban, East Java, Indonesia, consisting of 375 men (75%) and 125 women (25%). Detection of anti-HCV antibody prevalence in the blood donors was done by using Enzyme-Linked Immunosorbent Assay (ELISA).  The detection of HCV RNA in blood donors  sera  with positives  anti-HCV were performed using nested-PCR technique with three set of primer pairs targeting the NS5B and 5’UTR regions of HCV.  Further amplification products were visualized on a 2% agarose gel containing ethidium bromide under  UV illumination.Result: Seven positives anti-HCV blood donors sera (1.4%) were obtained from 500 blood donors sera, in PMI Tuban, East Java, Indonesia. Out of seven blood donors sera with positives anti-HCV, 6 sera (85,7%)  were positives HCV RNA by using nested PCR technique. Five HCV RNAs were detected with two set of primers based on NS5B HCV region and 1 positive HCV RNA was detected with another set of primer based on 5’UTR HCV region.Conclusion: HCV RNAs were detected in 85,7% positives anti-HCV blood donors using nested PCR technique, in PMI Tuban, East Java. Further research is needed to determine the  genotype  and  subtype  of  HCV. 
Co-Authors Cahyo Wibisono Nugroho Djoko Santoso Eka Arum Cahyaning Putri Haerani Rasyid Ika Nindya Kadariswantiningsih Maulana Antiyan Empitu Maulana Muhtadin Suryansyah Maulana Muhtadin Suryansyah MOCHAMMAD THAHA Mohammad Yusuf Alsagaff Mohammad Yusuf Alsagaff Nurina Hasanatuludhhiyah Retno Handajani Rieza Rizqi Alda Rieza Rizqi Alda Supiana Dian Nurtjahyani Yusuke Suzuki Yusuke Suzuki Zaky El Hakim Zaky El Hakim