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Sandy Vitria Kurniawan
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Health Professions Medicine & Pharmacology Public Health

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Journal : Damianus Journal of Medicine

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ANALISIS METODE PRESERVASI SUBKULTUR BERKALA DAN DENGAN AIR STERIL UNTUK JAMUR Aspergillus sp. DAN Candida albicans Gabi Vania Sally; Sandy Vitria Kurniawan; Sem Samuel Surja
Bahasa Indonesia Vol 19 No 2 (2020): Damianus : Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v19i2.1193

Abstract

Introduction: A good and proper preservation can maintain all collection of culture. Periodic subculture preservation is an old technique that is less able to guarantee the genetic characteristic for a long time. Sterile water is an alternative method in various studies to ensure the genetic characteristic for a long time and can be done in a simple laboratory concept. This study aimed to examine the method of periodic subculture and with sterile water Methods: This research was a descriptive study with an experimental laboratory design on Aspergillus sp. and Candida albicans. This study examined at periodic subculture methods and with sterile water in maintaining viability, level of contamination, morphology, and antifungal resistance after six months Results: The fungus had lived after being preserved with periodic subcultures without contamination, morphological changes, and resistance. Aspergillus sp. and Candida albicans also lived after being preserved with sterile water for six months with the same morphology and without contamination. The zone of inhibition of Aspergillus sp. had decreased but in Candida albicans remains the same. Conclusion: Preservation with periodic subculture and with sterile water can be applied to Aspergillus sp. and Candida albicans.
UJI SINERGITAS ANTIFUNGAL FLUCONAZOLE DENGAN MINYAK ATSIRI CYMBOPOGON CITRATUS TERHADAP JAMUR CANDIDA ALBICANS Sharon Claudia Ethelyn Sihole; Sandy Vitria Kurniawan
Bahasa Indonesia Vol 20 No 2 (2021): Damianus Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v20i2.2513

Abstract

Introduction: Candida albicans is a common human flora. However, if the amount is above average, it can cause a disease called candidiasis. Fluconazole is an antifungal medication that can cure candidiasis but its number of resistance is increasing. Therefore alternative treatments are needed like using herbs. Cymbopogon citratus essential oil is proven to have an antifungal effect. The purpose of this study is to see the synergistic antifungal effect of fluconazole combined with lemongrass against C. albicans. Methods: Experimental study design was used in this research and conducted in vitro. C. citratus was extracted with hydrodistillation method. Disc diffusion method was performed using MHA agar with Glucose 2% and methylene blue. Lemongrass essential oil concentration that are used are 2,5%, 5%, 10%, and 20% using tween as a solvent. Results: Minimum concentration of lemongrass essential oil that could inhibit C. albicans growth is 10% with average diameter of inhibition zone 10 mm. Minimum concentration of fluconazole combined with lemongrass essential oil that could inhibit C. albicans growth is 2,5% with average diameter 21,3333 mm. Average diameter of inhibition zone by fluconazole combined with lemongrass essential oil is greater than fluconazole’s inhibition zone added with lemongrass essential oil inhibition zone alone, therefore fluconazole combined with C. citratus essential oil work synergistically. Conclusion: There is a synergistic antifungal effect of fluconazole combined with C. citratus essential oil against C. albicans. Key Words: Candida albicans, Cymbopogon citratus, fluconazole, synergistic
Co-Authors Gabi Vania Sally Sem Samuel Surja Sharon Claudia Ethelyn Sihole