Infectious disease ranks first in the cause of morbidity in the developing countries including Indonesia especially in the eastern part of Indonesia. One of theleading infectious disease is hepatitis B that ranks ninth in the most leading cause of death. There are various cause of the spread of the disease includinglack of understanding in the infection measures including by the service giver of salon. Infection prevention behavior was caused by its service salon. Thepurpose of this study was to know the behavior in preventing infection of salon service giver in Semarang municipality. The study was a cross sectionalstudy, the independent variables were age, education, knowledge, attitude, infrastructure, supervisor support, and attitudes of service giver friend. Whilethe dependent variable was the behavior in the infection prevention measures. The sample was 50 salon service giver conducted with a purposive samplingmethod. The data analysis used chi-square and logistic regression. The result showed that there was a relationship between age, education, service giverfriend and infection prevention behavior, but there was no relationship between knowledge, attitude, infrastructure, supervisor support and infectionprevention behavior. The most influencing factor in infection preventing behavior was partner support of salon service giver (p=0,0002;OR =45.5). It isrecommended that salon owner provide tools used in accordance with the requirements/number of customers and make sure that to always take steps toprevent infections such as using schort, gloves.