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All Journal Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA MEDIA BINA ILMIAH Biosaintifika: Journal of Biology & Biology Education Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology)

Sri Isdadiyanto

Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Author-ID : 288951

Agriculture, Biological Sciences & Forestry Arts Humanities Education Health Professions Mathematics Medicine & Pharmacology Public Health Social Sciences

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Blood Lipid Profile of Coturnix coturnix japonica Fed whith Organic Feed and Supplement Curcuma longa Aetin, Entin Nur; Saraswati, Tyas Rini; Isdadiyanto, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11225

Peoples concern about quail egg consumption has triggered the efforts to produce quail eggs with low cholesterol content by using organic feed. Organic feed is one type of feed rations made of mixing organic material. In this study, standard organic feed (rice bran, yellow corn, soybean, and fishmeal) and organic feed containing mackerel, cassava leaves, and turmeric powder was administere to female japanese quail. Organic feed containing mackerel, cassava leaves, and turmeric powder can affect the Japanese quail (Coturnix coturnix japonica L.) lipid profile. This research used experimental methods. Twenty-seven female Japanese quails were divided into 3 treatment groups (PO: commercial feed, P1: standard organic feed, P2: feed containing organic turmeric powder, cassava leaves, and mackerel) for 145 days with 9 repeat. Data obtained were in the form of average daily feed intake and blood lipid profiles of each treatment group. Further data analysis was performed by analysis of variance (ANOVA) followed by Duncan test. The results showed that administration of organic feed containing mackerel, cassava leaves, and turmeric powder can reduce the levels of cholesterol, triglycerides, and LDL but increase the HDL levels in Japanese quail. Supplementation of turmeric powder improve the chemical quality of Japanese quail eggs, so it is good for the development of quail embryos as well as for consumption. It can be concluded that organic feed and turmeric powder supplementation stabilize blood lipid profile and improve the reproduction of Japanese quail egg low cholesterol, so good for the development of embryo quail and for consumption.

Rasio Otot-Tulang Pectoralis Berbagai Jenis Itik Lokal di Jawa Tengah Savitri, Ajeng Fatria; Wachidah Yuniwarti, Enny Yusuf; Isdadiyanto, Sri
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Local ducks farm businesses potentially developed as the meat producer, which was expected to fit and complete the nutrition for society. The provision of excellent meat quality was one of the main goals that integrated with the development of animal husbandary. The quality and quantity of meat on the carcass could be seen as one of the indicators of ducks productivity. The study about the influence of various types of ducks to pectoralis meat bone ratio as the component of commercial carcass had to be done to find out about the quality and genetics potentials of every ducks type in order to improve the quality of food. This study aimed to analyze the influence of various types of ducks in Central Java, such as Pengging duck, Tegal duck, and Magelang duck to the carcass quality evaluated from the value of pectoralis meat bone ratio. The ducks used in the study were 6 months. The types of ducks were used as comparison. Samples were taken from the three types of local ducks and the repetition was done six times for each type of ducks. The measured variables were live weight, pectoralis weight, pectoralis percentage, meat bone percentage, and meat bone ratio. The data were analyzed using ANOVA. Real different datas were analyzed by further testing Least Significant Difference Test (LSDT) at the level of 95%. The results showed that various types of local ducks did not significantly affect the value of the pectoralis meat bone ratio, therefore it could be concluded that various local ducks did not affect on pectoralis meat bone ratio.Â Keywords: Local ducks in Central Java, carcass, pectoralis meat bone ratio.

Profil Kadar Kolagen Kulit dan Tulang Tikus Wistar pada Berbagai Umur yang Mendapat Perlakuan Stres Oksidatif Hiperkolesterolemia dan Oleoresin Kulit Batang Kayu Manis (Cinnamomum sp) Sunarno, Sunarno; Isdadiyanto, Sri
Bioma : Berkala Ilmiah Biologi Vol. 12, No. 2, Tahun 2010
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Oleoresin cinnamon bark belonging to the phenolic compounds that are known to have potential as antioxidants. On the basis of the potential of these compounds may be used to prevent or repair tissue damage, whether caused by the factors of age and condition of oxidative stress hypercholesterolemia. This study aimed to obtain skin and bone collagen profile of Wistar rats at various ages after oxidative stress treated hypercholesterolemia and oleoresin from the bark of cinnamon (Cinnamomum sp). This study used 54 male Wistar rats with body weight (200 Â± 5 g), which are grouped into six treatment groups combined with the three age groups of mice that is three, six or nine months with three replications. Each group received treatment oleoresin and hypercholesterolemia. Giving oral oleoresin conducted on rats given oral way during the 7 days with a dose of 12 rats mg/200-gr bw / day. Hypercholesterolemic rats was conducted by feeding cholesterol content within 1% during the two months. The results showed that the profile of skin and bone collagen content of the highest found on threemonth old rats treated with oleoresin, oleoresin hypercholesterolemia and given again (P6, 2), namely 57.44 tg / mg and 33.47 tg / mg, while the profile of skin and bone collagen content of the lowest found in rats aged 9 months to get treatment without treatment of hypercholesterolemia oleoresin (P2, 9), namely 28.26 tg / mg and 10.65 tg / mg. From this research can be concluded that the condition of hypercholesterolemia and duration of the aging effect on skin collagen levels decrease and bone, and vice versa oleoresin at the age of young rats that received the treatment of oxidative stress could repair or prevent hypercholesterolemia decreased content of skin collagen and bone. In general, the rats are treated oleoresin and young age profile of the collagen content of skin and bones better compared with rats that do not get treatment in conditions of oxidative stress oleoresin hypercholesterolemia.

Struktur Histologi Hepar Tikus Wistar (Rattus norvegicus) Jantan setelah Pemberian Teh Kombucha Konsentrasi 75% dengan Waktu Fermentasi yang Berbeda Isdadiyanto, Sri; Tana, Silvana
Bioma : Berkala Ilmiah Biologi Vol. 21, No 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

This research was conducted to study and analyze the influence of kombucha fermentation 6 (P1), 9 (P2) and 12 (P3) days fermentation with 75% concentration on hepatic tissue structure and hepatocyte diameter. This study used 16 male Wistar rats consisted of 4 treatments and 4 repeat. The treatment of this study consisted of P0: control treatment; P1, P2 and P3 were treatment of kombucha fermented tea 6, 9 and 12 days of 75% concentration respectively. This research used Completely Randomized Design (RAL). The variables measured in this study were hepatocyte diameter, body weight, feed and drink consumption in animal test. The data obtained were analyzed using Analysis of Variance (ANOVA) at 95% confidence level. The result of data analysis showed that kombucha fermentation 6, 9 and 12 days of 75% concentration had no significant difference to all variables. Histology of the liver were not indicate the presence of tissue-damaged features such as chronic inflammation (cirrhosis). It could be concluded that tea kombucha was safe for consumption by mammalia because it was not affect liver damage.

Ratio Kadar Ldl/Hdl Tikus Putih Sprague Dawley Hiperlipidemia Setelah Diberi Cangkang Udang Laut (Penaeus monodon F.) Isdadiyanto, Sri
Bioma : Berkala Ilmiah Biologi Vol. 17, No.2, Tahun 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

The objective of this study was to analyze the ratio of LDL/HDL level of Sprague Dawley rats induced by high fat ration after given of sea shrimp carapace (Penaeus monodon F.). The animals for this study were twenty adult male rats divided into four groups, i.e. group I as the control was fed with basal ration containing normal fat for 3 months, group II was fed ration containing high fat for 3 months, group III was fed ration containing high fat and given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 3 months, group IV was fed ration containing high fat for 3 months and after 1 month given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the blood were collected to analyzes ratio of LDL/HDL level. LDL level and HDL level were measured using colorimetric enzymatic method and Roche/Hitachi cobas c systems automatically calculate. Â The difference between treatments was statistical analysis by Anava, and continued by Lowest Significant Difference Test to locate the difference. The result showed that high fat diet increased ratio of LDL/HDL level and Sea shrimp carapace decreased ratio of LDL/HDL level. Â Keywords: Sea shrimp carapace, Sprague Dawley rats, ratio of LDL/HDL level

Potensi Cangkang Udang Laut (Penaeus monodon F.) Terhadap Profil Lipid Tikus Putih Hiperlipidemia Isdadiyanto, Sri
Bioma : Berkala Ilmiah Biologi Vol. 17, No.1, Tahun 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

The objective of this study was to analyze the potency of sea shrimp carapace (Penaeus monodon F.) on lipid profiles of Sprague Dawley rats induced by high fat ration. The animals for this study were twenty adult male rats divided into four groups, i.e. group I as the control was fed with basal ration containing normal fat for 3 months, group II was fed ration containing high fat for 3 months, group III was fed ration containing high fat and given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 3 months, group IV was fed ration containing high fat for 3 months and after 1 month given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the blood were collected to analyzes lipid profiles. Lipid profiles were measured using colorimetric enzymatic method and Roche/Hitachi cobas c systems automatically calculate. Â The difference between treatments was statistical analysis by Anava, and continued by Lowest Significant Difference Test to locate the difference. The result showed that high fat diet decreased HDL level, but increased total cholesterol level and LDL level. Sea shrimp carapace increased HDL but decreased total cholesterol level and LDL level in rats by high fat induction. Â Keywords: Sea shrimp carapace, Sprague Dawley rats, lipid profiles

Glikogen Otot Rangka Ayam Broiler (Gallus gallus) setelah Pemberian Teh Kombucha dalam Air Minum Fathoni, Nasrul; Djaelani, M Anwar; Isdadiyanto, Sri
Bioma : Berkala Ilmiah Biologi Vol. 20, No 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Kombucha tea beverage obtained by fermenting sweetened green tea for 12 days with Acetobacter xylinum and Saccharomyces which produce various kinds of organic acids, vitamins, and acts as a probiotic. The role of kombucha tea as a growth promoter is to that improve the metabolic process in the digestion of broiler chickens, so that nutrients can and fulfilled optimally for growth and development. The aim of this research was to analyze the sceletal muscle glycogen glycogen (Gallus gallus) after treat with kombucha tea in drinking water. Kombucha tea used is the result of fermentation of green tea for 12 days. This research used DOC chickens (Day Old Chicken) as much as 20 randomly divided into 4 treatments with concentration ie 0%, 10%, 20%, and 40% kombucha tea in drinking water for 32 days. The variables measured were skeletal muscle glycogen levels, body weight, feed consumption and drink consumption. The data obtained were analyzed using ANOVA followed by Duncan Test with 95% confidence level using SPSS 17.0 software. The results of this study showed that kombucha tea on skeletal muscle glycogen and feed consumption showed no significant difference, while on body weight and drink consumption showed significantly different results. Based on the results of the study, it can be concluded that giving kombucha tea in drinking water to a concentration of 40% has not been able to affect the skeletal muscle glycogen in broiler chickens.Â Key words: Kombucha tea, broiler chicken, glycogen skeletal muscle

Pengaruh Waktu Fermentasi Teh Kombucha Kadar 50% Terhadap Tebal Dinding Dan Diameter Lumen Arteria Koronaria Tikus Putih Isdadiyanto, Sri
Bioma : Berkala Ilmiah Biologi Vol. 20, No 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

The objective of this study was to analyze the influences of 50% kombucha tea level on wall thickness and lumen diameter of coronary artery in rats. Sixteen male Rattus norvegicus rats, 2 months of age were used as experimental animals, with treatment kombucha tea that has been fermented for 6, 9 and 12 days at the temperature of 25oC per oral. The research design used to a completely randomized with 4 treatment (for 4 weeks) and 4 replications, i.e., P0 = control, without of kombucha tea, P1 = water + 1,8 ml kombucha tea that has been fermented for 6 days in the morning and afternoon, P2 = water + 1,8 ml kombucha tea that has been fermented for 9 days in morning and afternoon, P3 = water + 1,8 ml kombucha tea that has been fermented for 12 days in morning and afternoon. Variables measured were wall thickness and lumen diameter of coronary artery. Data were analyzed with ANOVA followed by Duncan test at 95% confidence level were performed using SPSS version 10,0. The result showed that kombucha tea did not influences the wall thickness and lumen diameter of coronary artery in rats.Â Key words:Â Â Rattus norvegicus, 50% kombucha tea level, wall thickness, lumen diameter, coronary artery

Perbandingan Kualitas Daging Itik Magelang, Itik Pengging Dan Itik Tegal Hidayati, Nuke Nur; Yuniwarti, Enny Yusuf Wachidah; Isdadiyanto, Sri
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Local ducks in Indonesia there was several kinds, for example Tegal ducks, Magelang ducks and Pengging duck each having morphology different of duck. Research on the quality meat of ducks that includes ash content, water content, carbohydrate content, fat content, protein content, and muscle cell diameter of femoral ducks important to know quality meat of each duck. This study aimed to analyzed the differences types of ducks on the chemical content of meat and femoral muscle histology of diameter cell at Tegal ducks, Magelang ducks and Pengging ducks. Ducks were used in this study maintained in the Central Breeding and Raising Ducks Work Unit Non Ruminant Livestock Banyubiru, Ambarawa. The duck six month old was analyzed chemical content of meat and measuring diameter of femoral muscle cells. Duck samples taken from three different types of ducks that Tegal duck,Pengging duck and Magelang duck, of each type of sample taken many ducks as 6 tails. Data were analyzed using analysis of variance (ANOVA). If the data were analyzed contained a real difference, continued by the Least Significant Differences Test (LSDT) at 95% confidence level. The results showed that no significant difference in all variables, from the result has been concluded that differences type of ducks not affect the chemical contents of duck meat and diameter of femoral muscle cells.Â Keywords: Local Ducks in Central Java, Quality of Meat, Muscle Cell Diameter Femoral Ducks.

Struktur Mikroanatomi Testis Tikus Putih (Rattus norvegicus) Setelah Pemberian Teh Kombucha Konsentrasi 75% Waktu Fermentasi 6, 9 Dan 12 Hari Isdadiyanto, Sri; Tana, Silvana
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

The objective of this study was to analyze the testicular microanatomy structure of Rattus norvegicus after treatment by 75% kombucha tea with variation of fermentation time. Sixteen male of rats, 2 months of age were used as experimental animals treated with kombucha tea that had been fermented for 6, 9 and 12 days at the temperature of 25oC per oral. This research was using completely randomized design with 4 treatment (for 4 weeks) and 4 replications, namely: P0 = control, without of kombucha tea, P1 = water + 1,8 ml kombucha tea that had been fermented for 6 days in the morning and afternoon, P2 = water + 1,8 ml kombucha tea that had been fermented for 9 days in morning and afternoon, P3 = water + 1,8 ml kombucha tea that had been fermented for 12 days in morning and afternoon. The variables measured were seminiferous tubule diameter and Leydig cell counts. Data were analyzed with ANOVA followed by Duncan test at 95% confidence level were performed using SPSS version 16,0. The results showed that kombucha tea decreased the diameter of the seminiferous tubule, showing the potention of spermatogenesis disturbance, but did not affect Leydig cell counts of Rattus norvegicus

Co-Authors Aetin, Entin Nur Agung Janika Sitasiwi Dina Arumsari, Dina Djaelani, M Anwar Enny Yusuf Wachidah Yuniwarti Enny Yusuf Wachidah Yuniwarti Fathoni, Nasrul Fitriani, Ratih Nur Jannah, Baiq Miftahul Nuke Nur Hidayati, Nuke Nur Rohman, Jihadul Hanif Fadlur Savitri, Ajeng Fatria Shinta Dwi Kurnia Silvana Tana Siti Muflichatun Mardiati Sunarno Sunarno Tyas Rini Saraswati Wulan Tri Lestari, Wulan Tri

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