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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia Buletin Peternakan Jurnal Agripet Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak dan Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Ilmu dan Teknologi Peternakan Tropis JSDH Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Animal Production : Indonesian Journal of Animal Production Tropical Animal Science Journal ARSHI Veterinary Letters International Journal of Animal Science Wahana Peternakan Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Kedokteran Hewan Journal of Animal Research and Applied Science

Jakaria Jakaria

Bogor Agricultural University

Author-ID : 290981

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

Published : 76 Documents Claim Missing Document

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Heritability estimation and environmental factors affecting production traits of Indonesian Ongole cross Hartati, .; Muladno, .; Jakaria, .; Priyanto, R.; Gunawan, A.; Aryogi, .; Talib, Chalid
Indonesian Journal of Animal and Veterinary Sciences Vol 20, No 3 (2015): SEPTEMBER 2015
Publisher : Indonesian Animal Sciences Society

Productivity data from 560 head of PO cattle have been collected for 9 years from 2004 until 2013 for evaluating heritability estimation and environmental factors affecting production traits of Indonesian Ongole cross from birth to yearling old. Heritability estimation were analyzed using varians analysis with data of paternal halfsib correlation. Varians component for to estimate of heritability value were analyzed using completely randomized design one - way classification. While a general linear model was used to analyze the environmental factors (sex, year, parity, season and type of births). The results showed that sex of calves and year of births had significant differences (P<0.05) for all production traits such as weight at birth, weaning and yearling, while the type of birth only presented a significant difference on birth weight. Parity, season and generation exhibited nonsignificantly effect on those production traits. Estimation of heritability on birth weight, weaning weight and yearling weight were 0.28+0.12; 0.47+0.15 and 0.63+0.17 respectively. The highest heritability values obtained in yearling weight, this means that yearling weight will be effective as selection criteria to improve the genetic of PO cattle. Key Words: Genetic, Non Genetic, Production, PO Cattle

Application of Linear Body Measurements for Predicting Weaning and Yearling Weight of Bali Cattle Gunawan, A; Jakaria, Jakaria
ANIMAL PRODUCTION Vol 12, No 3 (2010): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.875 KB)

The objective of this research was to predict the weaning and yearling weight of Bali cattle using simple linear body measurement. The height wither (HW), body length (BL) and heart girth (HG) were measured in centimeters, using caliper, whereas weaning (WW)and yearling weight (YW) was determined in kilograms using a weighing scale. Results of the correlation coefficient showed Â that hearth girth (HG) highly correlated with weaning and yearling weight were 0.847 and 0.871 respectively. In all, the height wither (HW) had the least correlation coefficient were 0.328 and 0.782 on weaning and yearling weight respectively.Â Results of the stepwise regression showed that HG was a good estimator of WW and YW of Bali cattle followed by HW and BL. The comparison of residuals indicated that only two equations (HG and BL) accurately predicted weaning and yearling weight. From these results, it was concluded that weaning and yearling weight of Bali cattle can be estimated using simple linear body measurement of heart girth, body length and height wither especially in most remote areas where farmers are challenged with the unavailability of weighing scale. (Animal Production 12(3): 163-168 (2010)Key Words: weaning and yearling weight, linear body measurements, Bali cattle

The Ghrelin Receptor (Ghsr) Gene Polymorphism in Indonesian Local Chicken and Crossbreed Is Associated With Carcass Traits Khaerunnisa, Isyana; Jakaria, Jakaria; Arief, Irma Isnafia; Budiman, Cahyo; Sumantri, Cece
ANIMAL PRODUCTION Vol 19, No 2 (2017)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Ghrelin receptor (GHSR) gene is candidate gene for growth performance in chicken by modulating growth hormone release from the pituitary by binding to its ligand of ghrelin. Ghrelin gene, or growth hormone secretagogue (GHS) gene, is well known as feed intake and energy homeostasis regulator in mammals and birds. The objectives of this study were to identify the polymorphism of the T1857C GHSR locus in Indonesian local chicken and to evaluate its effects on carcass traits. The gene polymorphism was identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using Hin6I restriction enzyme. Effect of genotype on carcass composition was analyzed using SAS General Linear Model (GLM) procedure. The genotyping was performed on 343 individuals including Merawang, Pelung,Â Sentul, Kampung, broiler (strain Cobb, parent stock), F1 crossbred of Kampung x broiler (strain Cobb, parent stock), and F2 crossbred of Kampung x broiler (strain Cobb, parent stock). All individuals were successfully amplified and were resulted in a 470 bp PCR product. This locus was polymorphic with two alleles (T and C) and three genotypes (TT, CT, and CC). The T allele and TT genotype were predominant in all populations. Individuals with CT genotype were significantly had higher live weight at 26w, carcass weight, commercial cuts weights, and muscles weights than TT genotype in F2 crossbred of Kampung x broiler population. Association of the T1857C GHSR locus-polymorphism with chicken carcass composition has been described in Indonesian chicken, providing evidence that GHSR might be an important candidate gene for chicken carcass traits.

The expression of heat shock protein 70 gene with organic selenium supplementation and its effetc on productivity of broilers in tropical environment Amizar, R.; Suharti, S.; Jakaria, J.; Mutia, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 4 (2017): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.4.279-287

The purpose of this experiment is to study the effect of organic selenium (Se) supplementation on the expression of heat shock protein 70 gene (HSP70), glutathione peroxidase (GSH-Px) enzyme activity, malondialdehyde (MDA) and productivity of broilers in tropical environment. Three kinds of environmental pens were designed in this experiment: comfortable environment pens with temperature of air conditioner adjusted at 22oC (R0), tropical environment pens (±30oC ) without organic Se (R1), and tropical environment pens supplemented with 0.30 ppm organic Se (R2). One hundred and twenty broiler chickens (unisex) were used in this study. There were 40 chicks per pen for each treatment. The experimental design was completely randomized with four replications for each treatment. The data were statistically analyzed using the general linear model of SAS program. Results showed that R0 and R2 groups had significantly increased (P<0.05) feed intake, body weight, body weight gain, and decreased feed conversion ratio compared to R1 groups. Meanwhile, the expression of HSP70, GSH-Px enzyme activity and MDA of R2 groups and R0 groups were significantly lower (P<0.05) than that of R1 groups. It was concluded that the broilers given 0.30 ppm organic Se in tropical environment had similar productivity and expression of HSP 70 with broilers kept in comfortable environment.

Diversity of D-loop mitochondrial DNA (mtDNA) sequence in Bali and Sumba Ongole cattle breeds Jakaria, J.; Musyaddad, T.; Rahayu, S.; Muladno, M.; Sumantri, C.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 4 (2019): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.4.335-345

This study aimed to investigate the diversity of the complete sequence of D-loop mitochondrial DNA (mtDNA) in Bali and Sumba Ongole (SO) cattlebreeds. A total of 24 blood samples were collected from Bali cattle (19 heads) and SO cattle (5 heads), and were extracted and then analyzed to obtain the sequence of D-loop mt DNA.Multiple alignments of the whole sequence of D-loop mtDNA were determined using clustal W. Genetic distance was calculated using a p-distance method, while the genetic tree was constructed using neighbor-joining (NJ) based on MEGA 6. Haplotype number, haplotype diversity (Hd) and nucleotide diversity (Pi) were analyzed using DnaSP version 6. As a result, the sequence of D-loop mtDNA in Bali cattle (921-1119 bp) and SO cattle (913 bp) was reported to have 8 and 4 haplotypes. Hd and Pi of Bali cattle reached 0.625±0.139 and 0.0266±0.0145, respectively, which wwere different from that of SO cattle, namely 0.900±0.1610 and 0.0064±0.0015, respectively. Specifically, we found 22 bp-repetitive nucleotide in Bali cattle, existing 3-9 times with a length of 66-198 bp present in D-loop mtDNA. This unique feature did not exist in SO cattle. Genetic distance and genetic tree determined according to sequence in hypervariability (HV-1) region of D-loop mtDNA (166 bp) resulted in satisfied separation, successfully classifying Bos javanicus, Bos indicus, and Bos taurus cluster.

NOVEL SNP OF CALPAIN-1 (CAPN1) GENE AND ITS ASSOCIATION WITH CARCASS AND MEAT CHARACTERISTICS TRAITS IN BALI CATTLE Pratiwi, N.; Maskur, M.; Priyanto, R.; Jakaria, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 3 (2016): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.3.109-116

Calpain-1 gene (CAPN1) produces an calpain enzyme controlling structure of meat protein and tenderness. The aims of this study were to identify Single Nucleotide Polymorphism (SNP) in exon 5 and 6 of CAPN1 gene and its associate with carcass and meat characteristic traits in bali cattle. A total of 48 bali cattles from BPTU-HMT Bali Cattle, Bali Province were used in the research. SNP in exon 5 and 6 of CAPN1 gene were identify with direct sequencing using MEGA 5 program. Analysis of polymorphism was conducted by PopGen 1.32 software to identify frequencies of genotype, allele and Hardy-Weinberg equilibrium. The association of CAPN1 gene genotype with carcass and meat characteristic traits was analyzed using Generalized Linear Model (GLM) procedure of SAS. Sequencing analysis at exon 5-6 of CAPN1 gene in Bali cattle resulted in eight polymorphic SNPs. They are c.3669T>C, c.3854G>A, c.3881T>C, c.3899C>T, c.3908C>G, c.4002C>A, c.4021G>T and c.4037A>C. The SNPs c.3669T>C, c.3854G>A and c.3899C>T were significantly (P<0.05) associated with rump thickness (RT), rump fat thickness (RFT) and marbling score (MS), while SNP c.4037A>C was not significantly associated with carcass and meat characteristic traits. The SNPs were significantly associated with carcass and meat characteristic traits namely c.3669T>C, c.3854G>A and c.3899C>T. Those SNPs may be used as candidate marker for Marker Assisted Selection (MAS) in bali cattle.

THE USE OF MICROSATELLITE MARKERS TO STUDY GENETIC DIVERSITY IN INDONESIAN SHEEP Jakaria, J.; Zein, M.S.A.; Sulandari, S.; Subandriyo, S.; Muladno, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 1 (2012): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.1.1-9

The purpose of this research was to study genetic diversity in Indonesian sheep population usingmicrosatellite markers. A total of 18 microsatellite loci have been used for genotyping Indonesian sheep.Total sheep blood 200 samples were extracted from garut sheep of fighting and meat types, purbalinggasheep, batur sheep and jember sheep populations by using a salting out method. Microsatellite loci datawere analyzed using POPGENE 3.2 software. Based on this study obtained 180 alleles from 17microsatellite loci, while average number of alleles was 6.10 alleles (6 to 18 alleles) from fiveIndonesian sheep populations (garut sheep of fighting type, garut sheep of meat type, purbalingga sheep,batur sheep and jember sheep population). The average of observed heterozygosity (Ho) and expectedheterozygosity (He) values were 0.5749 and 0.6896, respectively, while the genetic differentiation forinbreeding among population (FIS), within population (FIT) and average genetic differentiation (FST)were 0.1006, 0.1647 and 0.0712, respectively. Genetic distance and genetic tree showed that Indonesiansheep population was distinct from garut sheep of fighting and meat types, purbalingga sheep, batursheep and jember sheep population. Based on this results were needed a strategy for conservation andbreeding programs in each Indonesian sheep population.

Polymorphism of 5’UTR myostatin gene indel (g.1256/TTTTA) and its association with body weight in Boerka crossbred goat Ismail, R.; Handiwirawan, E.; Elieser, S.; Jakaria, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 3 (2020): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.3.163-172

This study aimed to identify the variation of 5’UTR Myostatin (MSTN) gene and its association to body weight in Boer, Kacang, and Boerka goats. DNA samples were obtained from 149 heads of the goats from the Indonesian Goat Research Center, Sungei Putih, North Sumatera. Polymorphism identification was conducted by direct sequencing and PCR-RFLP with DraI as the restriction enzyme for indel g.1256/TTTTA. Analysis of variance for body weight was carried out using the General Linear Model (GLM). The 5’UTR MSTN gene|DraI was monomorphic in Kacang but polymorphic in Boer and Boerka. Genotype frequencies for Boer 0.40(AA), 0.43(AB), 0.17(BB); Kacang 1.00(AA); Boerka 0.53(AA), 0.37(AB), 0.10(BB). The allele frequencies for Boer 0.62(A), 0.38(B); Kacang 1.00(A); Boerka 0.72(A), 0.28(B), respectively. AB was the most frequent genotype among Boer, but AA was the most frequent in Kacang and Boerka. Indel g.1256/TTTTA has a significant effect (P<0.05) only on birth weight (BW) of Boer, but no significant effect on other bodyweight parameters both in Boer and Boerka.AA genotype has the highest BW (P<0.05) than AB, but it’s not significantly different from BB. Indel g.1256/TTTTA could be used as a genetic marker for the birth weight of Boer but not in Boerka goats.

IDENTIFICATION OF UTERIN MILK PROTEIN (UTMT) GENE IN BALI CATTLE USING DIRECT SEQUENCING Jakaria, J.; Saputra, F.; Paramitasari, K. A.; Agung, P. P.; Maskur, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 1 (2016): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.1.1-6

The objective of this research was to identify diversity of exon 5 UTMP gene fragment in Bali cattle using direct sequencing. The total 60 blood samples of Bali Cattle derived from BPTU Bali in Bali siland (20 heads), BPTU Serading in Sumbawa island (20 heads) and Village Breeding Center in Barru District South Sulawesi (20 heads) were used to evaluate their genetic diversity at exon 5 UTMP gene. The forward and reverse data sequences were analyzed using Bioedit program and alignment analysis was carried out using MEGA5 program. Meanwhile haplotype analysis was performed by DnaSPv5 program. The result showed that partial sequences in exon 5 UTMP gene had 16 haplotypes with the highest number of haplotypes ware found in VBC Barru district South Sulawesi (8 haplotypes). Moreover, the highest average of haplotype (h) and nucleotide (p) diversity were found in VBC Barru district South Sulawesi were 0.7949 and 0.0016, respectively. In addition, minisatellite insersion was found in exon 5 UTMP gene fragment on Bali cattle which are consist of 5'-CCA GTC ATG AAG AAG GCA GAG GTC GTC GTG CCG GCG AAA-3'. According to our results, haplotype and minisatellite variation in exon 5 UTMP gene fragment can be used as a candidate genetic marker specific for reproductive trait in the Bali cattle and for its strategy breeding program in the future.

POLYMORPHISM STEAROYL-COA DESATURASE (SCD) GENE AND ASSOCIATON WITH CHARACTERISTICS MEAT IN BALI CATTLE Alwiyah, A.; Naraini, H.; Agung, P. P.; Jakaria, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 4 (2016): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.4.188-195

Stearoyl-CoA desaturase (SCD) is an enzyme produced by SCD gene which is responsible for a conversion of saturated fatty acids (SFA) to mono-unsaturated fatty acid (MUFA) in adipose tissue. This enzyme affects the fats in intramuscular so having influence on marbling. The purpose of this study was to obtain the polymorphisms of the SCD gene and their associations with meat quality traits in Bali cattle. The number of samples used were 48 heads of cattle consisted of 24 bulls and 24 cows from BPTU-HMT Bali cattle in the province of Bali. The SCD gene has been amplified using forward primer 5’-ACC CCT TGG TGT GTG GTT GTT CTT C-3 ‘and reverses primer 5'-CCT GAC GAT ACT ATG TTT CTA CTT C-3'. The polymorphisms of the SCD gene were identified by direct sequencing method. Meat quality traits such as thick of longissimus dorsi (TLD), thick of back fat (TBF), thick of fat rump (TFR), thick of rump (TR), marbling score (MS), and the percentage of intramuscular fat (PIMF) were analyzed using the Veterinary Ultrasound Scanner. To determine Hardy-Weinberg equilibrium status, both allele and genotype frequencies were analyzed using GENEPOP program (V3.2). Association of the SCD gene SNP and meat quality traits was analyzed by GLM. This result showed that there were 5 monomorphic SNPs (c.10153A>G, c.10318C>A, c.10329C>T, g.10394G>A, g.10486A>C) and 3 polymorphic SNPs (g.10360G>A, g.10428C>T, g. 10487G>A) were in HW equilibrium. Association analysis showed that g.10428C>T SNP significantly affected marbling score (MS) and percentage of intramuscular fat (PIMF) (P<0.05). Based on these results, g.10428C>T SNP of the SCD gene may be used as a candidate marker to select meat quality traits in Bali cattle.

Co-Authors . Aryogi . Aryogi, . . hartati . Hartati A Gunawan A Saefuddin A. Alwiyah, A. A. Anggraeni A. Anggraeni A. Furqon A. Gunawan A. Gunawan A. Gunawan A. GUNAWAN A.A. Ketut Agung Cahyawan W Adefia Fahira Amin Wahyudiono Aminurrahman Aminurrahman Andi Baso Lompengeng Ishak Anggi Nurhardiyanti Munawaroh Annang Dwijatmiko Asep Gunawan B Brahmantiyo B Tappa B. Brahmantiyo Berry Juliandi Bramada Winiar Putra C Sumantri C. Budiman C. SUMANTRI C. Sumantri C. Sumantri C. Sumantri C. Sumantri Cahyo Budiman Cece Sumantri Chalid Talib Chalid Talib D Duryadi D. A. Astuti D. Anggrela D. E. W. T. Sihite Dairoh Dairoh Dairoh Dairoh Dairoh, D. DEWI APRI ASTUTI E Andreas E Sari E. Andreas E. Handiwirawan Edwar Edwar EDWAR EDWAR Eryk Andreas F Hasan F Saputra F. Saputra F. Saputra, F. F. W. Pramukti Fitra Aji Pamungkas Fitriani Eka Puji Lestari Fuadi Zulkipli Gunawan Sitanggang H Martojo H. Naraini, H. H. Nuraini Hasrul Bani Asharudin Henny Nuraini I I Arief I Khaerunnisa I Komang Gede Wiryawan I Made Londra I. I. Arief I. Inounu I. Inounu I. Khaerunnisa I. Khaerunnisa I. Suhendro Ida Bagus Gaga Partama Irma Isnafia Arief Isyana Khaerunnisa Jessy Mainidar K Suzuki K. A. Paramitasari, K. A. K. G. Wiryawan K. Listyarini K. Listyarini K. Listyarini K. Listyarini Karimah, S. N. Khasanah, H. Komang Alit Paramitasari M Kaomini M Ulfah M. A. Abuzahra M. A. Abuzahra M. Baihaqi M. J. Uddin M. J. Uddin M. J. Uddin M. Syamsul Arifin Zein M. Yamin M. Yamin M.S.A. Zein Mardilah Maryani Maryani Maskur Maskur Mohamad Yamin Mokhamad Fakhrul Ulum Mokhamad Fakhrul Ulum Muhammad Nico Ghossani Musyaddad, T. N. Pratiwi, N. N. Ulupi Niken Ulupi P. P. Agung, P. P. Prastya Idihastuti Priyambodo, Danang Putra, I. G. R. Putri Indah Ningtias Quraisy Zakky R R Noor R Sari R. Azis R. H. Mulyono R. Iis Arifiantini R. Ismail R. K. Rusli R. Misrianti R. Mutia R. Mutia R. OKTAVIANI R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Putri R. R. Noor R. R. Noor R. R. Noor, R. R. R.R. Noor Rachmawati, S. M. Restu Misrianti Ridho Kurniawan Rusli Rini Herlina Mulyono Rita Mutia Robi Amizar Ronny R Noor Ronny Rachman Noor Ronny Rachman Noor Rudy Priyanto Ruhama Maya Sari Rukmiasih Rukmiasih Rukmiasih Rukmiasih S S Mansjoer S. Elieser S. H. Wijaya S. Rahayu, S. S. Subandriyo S. Suharti S. Sulandari S. Y. Basril Saputra, E. A. Sari, D. A. Sony Hartono Wijaya Sri Sulandari Sri Wahyuni Siswanti Suhendro, I. Suhendro, Ikhsan Suprio Guntoro Sutikno Madnasri Sutikno Sutikno Sutikno Sutikno T I Amalianingsih T Suryati T. Rafian T. Suselowati T. Toharmat Teguh Rafian Toto Toharmat Tya I Amalianingsih Ulum, M. F. W. Bilyaro W. Manalu Wasmen Manalu Wenny Ladhunka Nur Aliyya Wike andre Septian Winni Liani Daulay Winni Liani Daulay Yureni Sahril Dedi Z Wulandari

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