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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal ILMU DASAR Jurnal Kedokteran Syiah Kuala e-Journal Pustaka Kesehatan Research Journal of Life Science Indonesian Journal of Biotechnology Journal of Agromedicine and Medical Sciences Mutiara Medika: Jurnal Kedokteran dan Kesehatan UNEJ e-Proceeding MNJ (Malang Neurology Journal) Health Notions Jurnal Pengabdian Pada Masyarakat Warta Pengabdian Multidiciplinary Journal Al-sihah: The Public Health Science Journal Manuju : Malahayati Nursing Journal Jurnal Kesehatan dr. Soebandi Majalah Kedokteran Andalas Medical Technology and Public Health Journal Jurnal Inovasi Teknik Kimia Jurnal ABDINUS : Jurnal Pengabdian Nusantara AloHA International Journal of Health Advancement (AIJHA) STRADA Jurnal Ilmiah Kesehatan
Erma Sulistyaningsih
Department Of Parasitology, Faculty Of Medicine, University Of Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Other

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Peningkatan Kemampuan Mengatasi Masalah Stunting dan Kesehatan melalui Pemberdayaan Masyarakat Desa Sukogidri, Jember Erma Sulistyaningsih; Parawita Dewanti; Pulong Wijang Pralampita; Wiji Utami
Jurnal Pengabdian Pada Masyarakat Vol 5 No 1 (2020)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (684.396 KB) | DOI: 10.30653/002.202051.244

Abstract

INCREASING THE ABILITY IN OVERCOMING STUNTING AND HEALTH PROBLEM BY COMMUNITY EMPOWERMENT IN SUKOGIDRI VILLAGE, JEMBER. Indonesia has a high prevalence of stunting, and Jember is one of the six districts in East Java province that contribute to stunting prevalence. To overcome the stunting problem, it needs a holistic strategy including health, education and economic aspects, therefore the community empowerment is an important approach. The purpose of this public service program was to increase the awareness and capability of the community to overcome stunting and health problem by community empowerment. Several activities were conducted in this community service program, i.e. by examining the health status of the children, pregnant woman, and high risk people, counseling and education about stunting and the effect on health status and also the effect for the next generation, education about the efforts to prevent stunting, provide training to prepare a healthy food and specific training for the cadres who responsible for the stunting-alleviation program. The impact of the activity was an increase the knowledge and awareness of the community about stunting. And there were several trained-cadres about stunting and its prevention.
The Relation between Malondialdehyde (MDA) and Histopatological Appearance in male Wistar Rats Model Fridayanti, Febrina Sylva; Sulistyaningsih, Erma; Sakinah, Elly Nurus
Journal of Agromedicine and Medical Sciences Vol 1, No 1 (2015)
Publisher : Medical Faculty of Jember University (Fakultas Kedokteran Universitas Jember)

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Abstract

Fractures are a serious health problem in Indonesia due to increasing prevalence. The healing process of fracture is disturbed by the oxidative stress that caused by imbalance quantity of free radical and antioxidant. An antioxidant such as polyphenol, which can be found in cocoa, is needed to suppress oxidative stress. The study aimed to investigate the effect of the ethanolic extract of cacao on fracture healing process in a rat model through MDA concentration and histopatological appearance. This study is in vivo experimental study with post-test only controlled group design. 30 male Wistar rats were randomized and divided into 5 groups. 1 group was rats without fractured. The negative control and three treatment groups were rats with fractured manually on left tibia under anesthesia and immobilized by bandage. The treatment groups treated with cocoa ethanolic extract in a dose of 125 mg/kgBW, 250 mg/kgBW, and 500 mg/kgBW orally for 21 days. The result showed that there was a significant different between the treatment groups and the negative control group on MDA concentration and histopatological appearance (p>0,05). The corelation between them were strong and had negative direction (R=-0,771). The study concluded that cocoa ethanolic extract had a positive effect to supress oxidation stress and increases the number of osteoblast on fracture healing process.   Key words: cocoa ethanolic extract, polyphenol, fracture healing process, oxidative stress
Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate Sulistyaningsih, Erma; Moeljopawiro, Sukarti; Subandono, Jarot; Artama, Wayan T.
Indonesian Journal of Biotechnology Vol 10, No 1 (2005)
Publisher : Universitas Gadjah Mada

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Abstract

Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the researchwas to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinanttechnology. Tachyzoite was grown in Balb/c mice in vivo. Messenger RNA was isolated from total RNA and itwas used to synthesis cDNA. Complementary DNA encoding GRA1 protein of tachyzoite Toxoplasma gondii localisolate was amplified and cloned in a prokaryote cloning vector. The recombinant GRA1-encoding gene was thendigesting using EcoRI restriction endonuclease and sequencing. The result showed that the recombinant GRA1-encoding gene consisted of DNA sequences encoding all signal peptide and mature peptide of GRA1 protein.Alignment of recombinant GRA1 sequence to gene encoding GRA1 protein of Toxoplasma gondii RH isolate showed100% homologous.Keywords: GRA1 protein, Toxoplasma gondii, tachyzoite, cloning, cDNA
Hubungan Sanitasi Lingkungan dengan Kejadian Infeksi Soil Transmitted Helminths pada Anak Sekolah Dasar di Jember Nur Aqmarina Kusumawardani; Erma Sulistyaningsih; Cicih Komariah
Pustaka Kesehatan Vol 7 No 1 (2019): Volume 7 No. 1, 2019
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v7i1.17591

Abstract

Poor environmental sanitation is suspected to be one of the causes of soil transmitted helminthes (STH) infection. Environmental sanitation consists of water sources, latrine, sewerage, garbage disposal facilities, and floor type. The objective of this study was to investigate the relationship between environmental sanitation and the incidence of STH infection in primary school children. This was an observational analytic study with cross-sectional design. The subjects were 80 students of two elementary schools in Jember. Stool samples were collected and examined by Kato-Katz method. Data of environmental sanitation was collected by questionnaire and direct observation. Kato-Katz examination showed that 9 samples (11,3%) infected by Ascaris lumbricoides. The majority respondents had not fulfilled requirements of good environmental sanitation. The result of Spearman test showed that there was a significant correlation between STH infection and latrine (p=0,02) but there were no significant relationship between STH infection and environmental sanitation (p = 0.165), gender (p = 0,669), age (p = 0,92), water source (p = 0.084), sewerage (p = 0.146), garbage disposal facilities (p = 0,728), and floor type (p = 0,065).
The Density of Dermatophagoides sp. in Households and its Correlation with the Score for Allergic Rhinitis in Jember, East Java Sulistyaningsih, Erma; Laili, Elisa Fadia; Abrori, Cholis
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 21, No 1: January 2021
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v21i1.7925

Abstract

The prevalence of allergic rhinitis (AR) in Indonesia is an estimated 24.3% and increasing each year. The majority cause of AR is house dust mites (HDMs) allergens, especially Dermatophagoides sp. The screening tool to estimate the prevalence and diagnose AF in a population setting is the Score for Allergic Rhinitis (SFAR) questionnaire. This study aimed to investigate the correlation between the density of Dermatophagoides sp. in households and the SFAR in Jember, East Java. The study enrolled 30 housewives as respondents, which were selected by stratified random sampling. Respondents were interviewed using the SFAR questionnaire. The dust sample from each house of the respondent was collected, the HDMs either Dermatophagoides sp. or others were identified and the density of Dermatophagoides sp. was calculated. Data were analyzed using the Pearson Test. Dermatophagoides sp. were found in 21 out of 30 samples and 11 out of 30 respondents had an SFAR of ≥ 7. The Pearson analysis showed the value of p = 0.555 and r = 0.136. Dermatophagoides sp. was the majority of species of HDMs, but there was no significant correlation between the density of Dermatophagoides sp. in households and the SFAR among housewives  in Jember.
Specific sequence of Plasmodium falciparum DBL domains associated with severe malaria outcome Sulistyaningsih, Erma; Fitri, Loeki Enggar; Loescher, Thomas; Berens-Riha, Nicole
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Duffy-binding like (DBL) domains of Plasmodium falciparum are believed to be involved in erythrocytes invasion and infected erythrocytes cytoadhesion during the blood stage of malaria infection. In Plasmodium falciparum, DBL domains found in the two different protein families; Erythrocyte Binding Ligand (EBL) including EBA-175, EBA-140, EBA-181 and EBL-1, and Plasmodium falciparum Erythrocyte Membrane Protein-1. The study aimed at investigating the specific sequence of Plasmodium falciparum involved in severe malaria outcome.Blood samples from severe and uncomplicated falciparum malaria cases from Papua and South Kalimantan province, Indonesia, were collected for DNA extraction. A dried blood on filter paper were used for RNA extraction. PCR was performed using UNIEBP primers and directly sequenced. Internal var D primers were designed according to the sequencing of the ~550 bp band produced by UNIEBP primers. The nucleotide sequences were analyzed by NCBI BLAST. Multiple bands ranging from nearly 250 bp to 1 kb were resulted from gDNA in all samples. Two isolates yielded bands of 450 and 525 bp, three isolates showed three bands additionally 250 bp, one isolate presented four bands additionally 800 bp and one isolate resulted one band additionally 1 kb.Amplification of cDNA from severe malaria cases produced one to four bands ranging from 250 bp to 700 bp, and no band observed from cDNA of uncomplicated malaria. Sequencing of the 418 bp bands matched with the eba-175 gene, the 316 bp determined as DBL1a domain and 486 bp band matched with the DBLg domain isolated from placenta of PAM’s Malawian woman. The expression of a 237 bp sequence corresponding to var D gene, was detected solely in severe malaria patients, implicating an association of gene expression and manifestation of severe malaria. Further characterization of the var D gene with a larger sample size is required to draw a definite conclusion. Keywords: DBL domains, Plasmodium falciparum, severe malaria, var D gene.
Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia Arifin, Samsul; Fitri, Loeki Enggar; Sujuti, Hidayat; Hermansyah, Bagus; Endharti, Agustina Tri; Burhan, Niniek; Candradikusuma, Didi; Sulistyaningsih, Erma; Tuda, Josef Sem Berth; Zein, Umar
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.11

Abstract

Indonesia is still included in high endemic area of malaria infection. Early detection as well as appropriate and quick treatment is needed to be able to prevent and treat malaria in Indonesia. Laboratory examination using a microscopic method is still used as the gold standard to diagnose malaria cases. However, the morphology similarity of some Plasmodium species and the number of parasites that can be seen under microscopy causes malaria diagnosis become difficult if only relying on microscopy diagnostic method. The purpose of this study is to analyze the sensitivity and specificity of nested PCR compared to microscopic examination in diagnosing malaria cases. A cross-sectional study has been carried out in some areas of Indonesia and the microscopic analysis as well as nest PCR was done in Laboratory of Parasitology and Laboratory of Central Biomedical Faculty of Medicine, Universitas Brawijaya, Malang East Java Indonesia. A total of 149 blood samples from patients with clinical symptoms of malaria had been obtained from Sumatra, Sulawesi and East Java during December 2011 to December 2013. From 149 sample, 81.9% samples were diagnosed malaria positive by microscopy examination, whereas the PCR results showed that 90.6% of samples were positive. Nested PCR sensitivity is 97.5%, and microscopy 88.2%. Nested PCR specificity is 40.7%, whereas microscopy 78.5%. PPV and NPV for nested PCR are 88,2% and 78.5% respectively, and for microscopy are 97.5% and 40.7% respectively. Nested PCR has a higher sensitivity than microscopy in diagnosing malaria and is able to detect mixed infection better than microscopic examination. However, it is statistically less specific than microscopy examination.
Analysis of Factors Affecting the Contamination of Entamoeba sp. cyst and Soil-Transmitted Helminths egg on Dug Well Water Iza Billa Fahmi; Erma Sulistyaningsih; Dion Krismashogi Darmawan
Journal of Agromedicine and Medical Sciences Vol 7 No 2 (2021)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v7i2.22233

Abstract

Dug well water is often polluted by fecal waste containment such as septic tank and pit latrine system due to its distance. The common pathogenic parasites found in fecal waste containment are E. histolytica and Soil-Transmitted Helminths (STH). The port de entry of both parasites is ingestion of contaminated food or water by fecal materials. This study was aimed to analyze factors affecting the existence of Entamoeba sp. cyst and STH egg on dug well water in Bungatan Village, Situbondo. This observational analytic study used cross-sectional approach, carried out 30 respondents in Bungatan Village, Situbondo. Entamoeba sp. cyst and STH egg were examined using sedimentation method, the distance of fecal waste containment to dug well measured by measuring tape. The characteristic of fecal waste containment and dug well was observed using questionnaires. The results showed 100% fecal containment used pit latrine system, 100% had an incomplete well standard, 66,7% had unstandardized distance with fecal waste containment. Entamoeba sp. cyst and STH egg were not found on all water samples. The soil condition near the dug well (acidity, temperature, moisture, and sunlight intensity) and physical condition (temperature) of dug well water were inappropriate to the development of Entamoeba sp. cyst and STH egg. The dug well's component and its distance to fecal containment did not affect Entamoeba sp. cyst and STH egg existence in all dug well water. The physical soil condition near the dug well and the temperature of dug well water were inappropriate to the development of both parasites. Keywords: Entamoeba sp., Soil Transmitted Helminths, Dug Well, Fecal Waste Containment
Pengaruh Pemberian Ekstrak Etanol Kakao (Theobroma cacao) terhadap Kadar Malondialdehida Tikus Wistar Jantan Model Fraktur Tulang (The Effect of Cocoa Ethanolic Extract (Theobroma cacao) on Malondialdehyde of Male Rattus novergicus Bone Anas Bakhtiar Diyansah; Erma Sulistyaningsih; Muhammad Hasan
Pustaka Kesehatan Vol 3 No 1 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Fracture is a serious musculoskeletal trauma. The healing process of fracture is disturbed by the oxidative stress, because of imbalance quantity of Reactive Oxygen Species (ROS) and an antioxidant. An antioxidant such as polyphenol, that can be found in cocoa, is needed to suppress oxidative stress. The study aimed to investigate the effect of cocoa ethanolic extract on decreasing oxidative stress by measuring malonaldialdehyde (MDA) concentration in fracture healing process. Trial test were conducted in eleven rats to determine appropriate plaster technique. The study used thirty rats and divided into five groups; the normal group without fracture, the negative control group and three treatment groups with manually fractured on left tibia under anaesthesia and immobilized by leucodur plaster. Each treatment group was treated with cocoa ethanolic extract in a dose of 125 mg/kgBW, 250 mg/kgBW, or 500 mg/kgBW orally, respectively for 21 days. The result showed that there was a significant effect of cocoa ethanolic extract on serum and bone MDA concentration in which MDA concentration decreased after cocoa ethanolic extract treatment. The study concluded that cocoa ethanolic extract had a positive effect on supression of oxidative stress in fracture healing process.   Keywords: Cocoa ethanolic extract, polyphenol, fracture healing process, MDA, ROS
Efektivitas Ekstrak Artemisia vulgaris L. sebagai Hepatoprotektor pada sel-sel Hati Tikus yang Diinduksi Niasin Laily Rahmawati; Erma Sulistyaningsih; Rosita Dewi
Pustaka Kesehatan Vol 6 No 2 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i2.7617

Abstract

The niacin in energy drinks has metabolic product that cause oxidative stress and liver damage, while the liver damage can be prevented by hepatoprotective agents. Scoparone in Artemisia vulgaris L. can act as a hepatoprotector by its antioxidant effect. This study aimed to investigate the effectivity of Artemisia vulgaris L. extract as a hepatoprotector in wistar hepatocytes induced by niacin. This study used 25 male rats which were divided into 5 groups: normal, the negative control, the positive control, the treatment group I, and II. Treatment was conducted for 28 days. The samples were terminated and the hepatocyte were prepared for histological examination. Histological appearance was catagorized as mild, moderate, and severe damage with or without inflamatory cells activity. The data analysis by Kruskal Wallis showed significant difference (p<0,001). Further analysis by Mann Whitney revealed significantly difference (p<0,05) between normal group and all groups, negative control group and positive control group, and positive control group and treatment group I, but not significantly difference between negative control group and treatment group I, negative control group and treatment group II, positive control group and treatment group II, and between treatment groups. The study concluded that the effectivity of Artemisia vulgaris L. extract has not been proven as a hepatoprotector but further study is needed to draw a definite conclusion. Keywords: energy drink, niacin, Artemisia vulgaris L., hepatoprotector
Co-Authors Agustina Tri Endharti Agustina Tri Endharti Al Munawir Aldi Cahya Muhammad Alfi Kamalia Aliyatul Farida Anas Bakhtiar Diyansah Ancah Caesarina Novi Marchianti Bagus Hermansyah Bagus Hermansyah Burhan, Niniek Cholis Abrori, Cholis Cicih Komariah Denaneer Rahmadatu Devita Prima Nurmasari Didi Candradikusuma Didi Candradikusuma Dini Agustina Dion Krismashogi Darmawan Dwi Wahyuni Dwita Aryadina Rachmawati Elly Nurus Sakinah, Elly Nurus Endang Sri Purwanti Wulandari Eqiel Navadz Akthar Alami Erfan Efendi Eva Sulawati Arum Fathul Hidayatul Hasanah Febrina Sylva Fridayanti, Febrina Sylva Fifil Sumarni Sumarni Firdha Novitasari Fitri Dika Maharani FX Ady Soesetijo, FX Ady Ghani Silahudin Hadi Prayitno Heni Fatmawati Hidayat Sujuti Hidayat Sujuti I Ketut Suada Ika Rahmawati Sutejo Intan Rachmawati Irawan Fajar Kusuma Iza Billa Fahmi Jarot Subandono Jarot Subandono Jauhar Firdaus Josef Sem Berth Tuda Kahfi Karunia Ilahi Laeli Kurniasari Laili, Elisa Fadia Laily Rahmawati Leersia Yusi Ratnawati Loeki Enggar Fitri Loeki Enggar Fitri Luh Putu Ratna Sundari Muhammad Hasan Ngurah Agung Reza Satria Nugraha Putra Nicole Berens-Riha Nicole Berens-Riha, Nicole Niniek Burhan Nur Aqmarina Kusumawardani Pralampita, Pulong Wijang Pungky Setya Arini Rina Fitriana Rina Fitriana Ristya Widi Endah Yani Rosita Dewi Salim Salim Samsul Arifin Samsul Arifin Sheilla Rachmania Silvi Ahmada Chasya Sinta Ariyani Sri Hernawati Sugiyanta Sugiyanta Sukarti Moeljopawiro Sukarti Moeljopawiro Thomas Loescher Thomas Loescher, Thomas Tristira Urvina Tuda, Josef Sem Berth Umar Zein Warda Ayu Nadira Wayan T. Artama Wayan T. Artama Widhi Dyah Sawitri Wiji Utami Winnie Almira Setyoadji Wiwien Sugih Utami Yunita Armiyanti