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All Journal JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi Biosel: Biologi Science and Education Jurnal Biosilampari: Jurnal Biologi Biofaal Journal Rumphius Pattimura Biological Journal

Amos Killay

Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Pattimura, Ambon

Author-ID : 298817

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Articles

Kitosan dari Limbah Kulit Kepiting Rajungan (Portunus sanginolentus L.) sebagai Adsorben Zat Warna Biru Metilena Tanasale, Matheis F.J.D.P.; Killay, Amos; Laratmase, Marsela S
Jurnal Natur Indonesia Vol 14, No 1 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The chitosan from shell of crabs (Portunus sanginolentus L.) waste was produced from deproteinization, demineralization, depigmentation,and deacetylation steps. This process was obtained 22.66% chitosan which identified by FTIR spectrophotometry and had 65.47% degree ofdeacetylation. This chitosan was used to adsorb methylene blue dye which fit to the Langmuir isotherm with 0.0312 l mg -1 equilibriumconstant of adsorption (K) and 4.20 mg g -1 maximum capacity of adsorption (q m).

Efek Antihiperurisemia Sirup Cengkeh pada Tikus Rattus Norvegicus Model Asam Urat Nia D. Laratmase; Maria Nindatu; Amos Killay; Adrien Jems Akiles Unitly
Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi Vol 13, No 2 (2021): Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jbe.v13i2.24279

Penelitian ini bertujuan untuk mengetahui efektivitas antihiperurisemia sirup cengkeh pada tikus Rattus norvegicus model asam urat dan dosis antihiperurisemia sirup cengkeh yang efektif. Penelitian ini menggunakan RAL yang terdiri dari 5 perlakuan dan 3 kali ulangan. Perlakuan adalah normal, kontrol negatif, kontrol positif, P1 dan P2. Pemberian sirup cengkeh dosis 1.8ml/ekor/hari (P1) dan dosis 3.6 ml/ekor/hari (P2) selama 14 hari digunakan untuk mengamati asam urat. Pengambilan data dilakukan sebanyak 3 kali yaitu setelah tikus diberi kalium bromat 0.87ml dan setelah diberi allopurinol dan sirup cengkeh. Hasil penelitian menunjukkan bahwa Pemberian sirup cengkeh memiliki efek antihiperurisemia yang sama dengan allopurinol yang mampu menurunkan kadar asam urat dalam darah sampai kebatas normal. Dosis sirup cengkeh 1.8ml dalam penelitian ini merupakan dosis yang baik sebagai antihiperurisemia.

Kandungan Karbohidrat, Lemak Dan Kalsium Kulit Buah Pisang Ambon Kuning (Musa paradisiaca var. sapientum (L.) Kunt) Pada Dataran Tinggi Dan Dataran Rendah Di Kecamatan Leitimur Selatan Kota Ambon Deli Wakano; Amos Killay; Wulandary Rasia Caling
BIOSEL (Biology Science and Education): Jurnal Penelitian Science dan Pendidikan Vol 10, No 2 (2021): BIOSEL (Biology Science and Education): Jurnal Penelitian Science dan Pendidika
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

The aim of this research is to find out the carbohydrate, fat, and calsium content of yellow Ambon banana (Musa paradisiaca var. Sapientum (L.) kunt) grown on South Leitimur lowland and highland, Ambon (as an attempt to convert banana peel into food). The research is a descriptive quantitative research. The research was conducted in Ambon Research and Standardization Center Laboratory on November 2016. The research measures physical and chemical factors such as height, soil pH, soil types, and temperature. Banana peels used in the research were taken from yellow ambon banana grown in two different areas: the lowland and the highland. The result shows that banana peels from the highland contain 4.07% carbohydrate, fat 0.82% and calcium 19.11 µg/gr, while banana peels from the lowland contain 4.08%, fat 0.85% and ca;cium 42.82 µg/gr. Keywords: Carbohydrate, fat, calcium, banana peel, yellow ambon banana, highland, lowland

UJI AKTIVITAS SEDIAAN SPRAY HAND SANITIZER KOMBINASI EKSTRAK DAUN KEMANGI DAN DAUN JERUK NIPIS SEBAGAI ANTIBAKTERI TERHADAP Staphylococcus aureus Idham Halil Zakaria; Cecilia Anna Seumahu; Amos Killay
Jurnal Biosilampari : Jurnal Biologi Vol 4 No 2 (2022): Biosilampari
Publisher : LP4MK STKIP PGRI Lubuklinggau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31540/biosilampari.v4i2.1514

Basil and lime leaf extracts have chemical compounds that function to inhibit the growth of Staphylococcus aureus and total bacteria on the palms of the hands. The purpose of this study was to determine the antibacterial activity of basil and lime leaf extracts against Staphylococcus aureus total bacteria on the palms of the hands. This experimental laboratory research used basil and lime leaves which were extracted using the partial maceration method. The formulation of the hand sanitizer spray combination of basil leaf extract with lime leaves is 1:2, 2:1, and 2:2. The formulation was tested for an antibacterial effect on Staphylococcus aureus and total bacteria on the palms. Data analysis used descriptive quantitative. The results showed that the extracts of basil leaves and lime leaves had an antibacterial activity of Staphylococcus aureus and total bacteria. Formulation 2:2 is the best formulation with bactericidal power of formula is 0 CFU/ml and total bacteria with antiseptic power of formula are 7 CFU. The study concluded that basil and lime leaf extracts were effective in killing Staphylococcus aureus and total bacteria on the palms of the hands.

ANALISIS FILOGENETIK SUBUNIT ALFA DNA POLIMERASE III DAN ERROR-PRONE DNA POLIMERASE DARI GENUS RHODOBACTER Edwin Thomas Apituley; Amos Killay
Biofaal Journal Vol 3 No 1 (2022): Biofaal Journal
Publisher : Jurusan Biologi FMIPA Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v3i1pp19-27

Sekuens nukleotida dari gen dnaE yang mengkodekan subunit alfa dari DNA polimerase III maupun sekuen asam amino produknya dari genus Rhodobacter dan beberapa genus yang berhubungan dianalisis untuk menentukan hubungan evolusioner mereka. Metode Maximum Likelihood dan model Kimura 2 parameter masing-masing untuk mengkonstruksi pohon filogenetik dan mengukur jarak genetik. Pohon filogenetik berdasarkan sekuen nukleotida dari gen yang mengkodekan subunit alfa dari DNA polimerase III dibandingkan terhadap pohon filogenetik berdasarkan sekuen gen 16S rRNA, Genus Rhodobacter maupun genus lain yang diuji memiliki 2 jenis dnaE, diberi nama dnaE1 dan dnaE2. Perbandingan pasangan sekuen menunjukkan bahwa persentase keidentikan sekuen antara kedua jenis gen dibawah 50 persen untuk sekuens nukleotida dan 25 persen untuk sekuen asam amino. Satu anggota genus Rhodobacter, R.sediminicola JA983 memiliki tiga salinan gen dnaE dengan rentang keidentikan 40 persen hingga 54 persen untuk sekuen nukleotida dan 22.6 hingga 50.6 persen untuk sekuen asam amino. Pohon filogenetik berdasarkan sekuen nukleotida dnaE memiliki dua percabangan utama, masing-masing mewakili dnaE1 dan dnaE2. Topologi dari pohon filogenetik berdasarkan gen dnaE memiliki kemiripan terhadap pohon filogenetik berdasarkan sekuen gen 16S rRNA, terutama untuk percabangan dnaE1. R.sediminicola memiliki satu salinan gen pada percabangan dnaE1 dan dua salinan gen pada percabangan dnaE2, pada pohon filogenetik berdasarkan sekeun gen dnaE, dan dianotasi sebagai dnaE2.1 dan dnaE2.2.

Kinetics of Formation and Characterization of Green Silver Nanoparticles of Ficus variegata Leaf Extract Synodalia C. Wattimena; Violin Ririmasse; Amos Killay; Philipus J. Patty
Jurnal Kimia Sains dan Aplikasi Vol 25, No 1 (2022): Volume 25 Issue 1 Year 2022
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

This study aimed to determine the formation rate of silver nanoparticles synthesized using leaf extract of Ficus variegata and characterize their physical, chemical, and antibacterial properties against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. For the formation rate determination, an empirical exponential model was proposed and used to fit the absorbance vs. time data (kinetics data). The surface plasmon resonance wavelength was measured using UV-Vis spectroscopy for physical and chemical characterization. The shape and size of the silver nanoparticles were characterized by transmission electron microscopy (TEM), and organic materials on the surface of the particles were identified by characterizing the associated chemical bonding using FTIR spectroscopy. For antibacterial assays, disc diffusion and spectrophotometric methods were used. The formation rates of the silver nanoparticles were 0.036 per hour or 1.0 x 10-5 s-1 (slower rate) and 0.767 per hour or 2.1 x 10-4 s-1 (faster rate). UV-Vis absorption spectrum indicated the surface plasmon resonance peak at 415 nm. Silver nanoparticles formed mainly were spherical, with a mean diameter of 26.5±0.7 nm. The FTIR spectrum indicated the presence of organic materials on the surface of the silver nanoparticles, which indicated the involvement of the extract as a reducing agent in particles formation. Antibacterial assay showed that synthesized silver nanoparticles inhibited the growth of both S. aureus and E. coli. The results from the disc diffusion method imply that the particles inhibited the growth of E. coli more effectively than S. aureus.

ADMINISTRATED OF SNAKE WOOD (Strychnos lucida) TO DECREASED ABNORMALITY SPERMATOZOA OF MICE (Mus musculus) DIABETES MELLITUS MODELING Marissa Beatrix Kunu; Chomsa Dintasari Umi Baszary; Amos Killay
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p037-042

Diabetes mellitus, known as diabetes or blood sugar disease, is a group of chronic diseases characterized by increased blood sugar level (hyperglycemia). Prolonged hyperglycemia conditions will cause an increase in ROS (Reactive Oxygen Spesies) production by the mitochondria, which then cause damage to the mitochondrial membrane resulting in loss of function of the mitochondrial membrane potential and can induce sperm cells apoptosis. This study aims to determine effect of kayu ular (Strychnos lucida) steeping on decreasing spermatozoa abnormalities of mice (Mus musculus) diabetes mellitus model. The result showed that administration of kayu ular steeping at concentration of 2,6gr/50ml was signicantly reduced (P<0.05) spermatozoa abnormality. This dose is the most effective concentration to decreased the spermatozoa abnormality of mice. This though to be due to the active compounds found in steeping kayu ular (Strychnos lucida) which can counteract free radicals due to diabetes mellitus that decreasing spermatozoa abnormalities.

SOATTING OF UREA FERTILIZER AND CONCENTRATION LEVEL OF SEAWEED CARRAGINAN Eucheuma cottonii Stefano Rijoly; Amos Killay; Joseph A Rupilu
RUMPHIUS Vol 2 No 1 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i1p025-031

Euchema cottonii seaweed as carrageenan-producing, growth and development is in need of the quality of light and nutrients such as nitrogen, such as urea. This study aims to determine the combination of long soaking and urea concentration levels that are effective in improving the content of Euchema carrageenan cottonii. This research method uses long immersion urea treatment 2 hours, 4 hours, 6 hours and without immersion (control) and urea concentration of 1 gram, 1,5 gram, 2 gram and wihout of urea fertilizer (control), repeat 3 times. The content of carrageenan in each treatment will be analyzed using ANOVA, followed by HSD test at 95% significance level. The results showed that the best carrageenan content (66.52%) in the treatment of long soaking 2 hours with 2 grams of urea concentration and carrageenan content of the lowest (51.33%) obtained in the treatment of long immersion 6 hours with 1 gram of urea concentration. Carrageenan seaweed Eucheuma cottonii on research generally meets the standards of quality carrageenan for maximum moisture content 35% (SNI Indonesia), and ash content of 15-40% (FAO, FCC), a maximum of 35% (EEC).

Co-Authors Adrien Jems Akiles Unitly CECILIA ANNA SEUMAHU Chomsa Dintasari Umi Baszary Deli Wakano Edwin Thomas Apituley Idham Halil Zakaria Joseph A Rupilu Marissa Beatrix Kunu Marsela S Laratmase Matheis F.J.D.P. Tanasale Nia D. Laratmase Nindatu, Maria Philipus J. Patty Stefano Rijoly Synodalia C. Wattimena Violin Ririmasse Wulandary Rasia Caling

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