Diana Chusna Mufida
Departement Mikrobiologi, Fakultas Kedokteran, Universitas Jember, Jl. Kalimantan No.37, Kampus Tegal Boto, Kabupaten Jember, Jawa Timur, 68121

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PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Diana Chusna Mufida; Yuna Annisa Salsabila; Enny Suswati; Bagus Hermansyah; Dini Agustina
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
Protein Haemaglutinin Outer Membran Protein (OMP) 35 kDa sebagai Protein Adhesin Proteus mirabilis pada Vesika Urinaria Kelinci Suswati, Enny; Mufida, Diana Chusna
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (89.982 KB) | DOI: 10.31258/jnat.12.2.136-142

Abstract

Proteus mirabilis is opportunistic and nosocomial pathogen that usually found in clinical specimen from patientswith catheter. The pathogenic mechanism of the bacteria are not fully elucidated especially its potential activity ofthe protein as hemaglutinin and adhesion molecule. The aim of this study is to evaluate the role of 35 kDa outermembrane protein from P. mirabilis. After identification, bacterial isolate of OMP fraction 12,5% SDS-PAGE wereused to isolate OMP followed by hemaglutinin test and invitro adhesion test. The study showed that the 35 kDa OMPof P. mirabilis was a hemaglutinin protein that could agglutinate mice erythrocytes, rabbit erythrocytes, and humangroup O erythrocytes. Hemaglutination test were negative on erythrocytes human blood group A,B, and AB. The 35kDa OMP was also adhesion protein showed by its activity to adhere to the rabbit vesica urinaria epithel receptor.The increase dose of 35 kDa OMP will decrease the amount of P. mirabilis bacteria to adhere to rabbit vesicaurinaria epithel (p< 0,05 ).
PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Mufida, Diana Chusna; Salsabila, Yuna Annisa; Suswati, Enny; Hermansyah, Bagus; Agustina, Dini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
Hubungan antara Higiene Perorangan dan Kejadian Koinfeksi Cacingan pada Penderita Tuberkulosis Shofia, Lailatis; Hermansyah, Bagus; Suswati, Enny; Agustina, Dini; Mufida, Diana Chusna; Shodikin, Muhammad Ali
Sriwijaya Journal of Medicine Vol. 4 No. 1 (2021): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/SJM.v4i1.153

Abstract

Tuberculosis (TB) and intestinal worms are infectious diseases with a large number of sufferers in Indonesia. The high prevalence of intestinal worms in Indonesia allows the occurrence of STH coinfection in pulmonary TB patients which causes anti M. tuberculosis immunity to decrease so that the response to tuberculosis treatment is not optimal. One of the risk factors of STH infection is personal hygiene. The purpose of this study was to analyze the relationship between individual hygiene and the incidence of STH coinfection in TB patients in Tempurejo Sub-District, Jember District. This type of research is observational research with cross sectional analytic design. The population used was TB patients in Tempurejo Sub-District in the period September - October 2019. The data used in this study were primary data in the form of interviews and results of stool examination. Stool examination is carried out using the sedimentation and floatation methods. Data analysis was performed using Fisher's exact test. The results of this study indicate that the incidence of STH co-infection in TB patients in Tempurejo Subdistrict is 9.67% and is caused by two STH species, A. lumbricoides (66.7%) and Hookworm (33.3%). Respondents' personal hygiene consisted of good hygiene (64.5%) and bad hygiene (35.5%), where 66.7% of cases of STH coinfection occurred in respondents with poor personal hygiene. Fisher's test results showed that there was no significant relationship between personal hygiene with the incidence of STH coinfection in TB patients in Tempurejo Sub-District, Jember District (p-value of 0.281).
Pengaruh Pemberian Imunisasi Intranasal Epitope Protein RrgB 255-270 Streptococcus pneumoniae Terhadap Kadar IL-4 Widiatmaja, Danang Tejamukti; Mufida, Diana Chusna; Febianti, Zahrah
Sriwijaya Journal of Medicine Vol. 4 No. 1 (2021): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/SJM.v4i1.155

Abstract

Streptococcus pneumoniae or pneumococcus is the main cause of community acquired pneumoniae (CAP). Infection of pneumococcal can be prevented by vaccines, like PPV and PCV. Those vaccines have several weaknesses, like, limited to several strain of bacteria and invasive. Nowadays, development of pneumococcal vaccines used the antigenic component of which occur on the bacteria, like epitope. One of the epitopes that can be used as vaccine candidate is epitope from RrgB protein of bacteria’s pili. One of the RrgB protein’s epitope that has been identified having antigenic property is epitope protein RrgB 255-270 of Streptococcus pneumoniae bacteria’s pili. That epitope will stimulate immune system to produce antibody against the antigen supported by cytokine, like IL-4. This research aim is to knowing the effect of intranasal immunization epitope protein RrgB 255-270 Streptococcus pneumoniae to IL-4 level. Wistar rat’s nasal rinse sample IL-4 levels measured by ELISA method. The mean of K1 is 28,852± 18 ng/L, K2 is 20.630 ± 9 ng/L and K3 is 18.519 ± 6 ng/L. Result of Welch ANOVA test shows that p-value of this research is 0.299. That can be concluded that the effect of intranasal immunization epitope protein RrgB 255-270 S. pneumoniae give non-significant difference (p-value > 0.05).
Hubungan antara Higiene Perorangan dan Kejadian Koinfeksi Cacingan pada Penderita Tuberkulosis Shofia, Lailatis; Hermansyah, Bagus; Suswati, Enny; Agustina, Dini; Mufida, Diana Chusna; Shodikin, Muhammad Ali
Sriwijaya Journal of Medicine Vol. 4 No. 1 (2021): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/SJM.v4i1.153

Abstract

Tuberculosis (TB) and intestinal worms are infectious diseases with a large number of sufferers in Indonesia. The high prevalence of intestinal worms in Indonesia allows the occurrence of STH coinfection in pulmonary TB patients which causes anti M. tuberculosis immunity to decrease so that the response to tuberculosis treatment is not optimal. One of the risk factors of STH infection is personal hygiene. The purpose of this study was to analyze the relationship between individual hygiene and the incidence of STH coinfection in TB patients in Tempurejo Sub-District, Jember District. This type of research is observational research with cross sectional analytic design. The population used was TB patients in Tempurejo Sub-District in the period September - October 2019. The data used in this study were primary data in the form of interviews and results of stool examination. Stool examination is carried out using the sedimentation and floatation methods. Data analysis was performed using Fisher's exact test. The results of this study indicate that the incidence of STH co-infection in TB patients in Tempurejo Subdistrict is 9.67% and is caused by two STH species, A. lumbricoides (66.7%) and Hookworm (33.3%). Respondents' personal hygiene consisted of good hygiene (64.5%) and bad hygiene (35.5%), where 66.7% of cases of STH coinfection occurred in respondents with poor personal hygiene. Fisher's test results showed that there was no significant relationship between personal hygiene with the incidence of STH coinfection in TB patients in Tempurejo Sub-District, Jember District (p-value of 0.281).
Pengaruh Pemberian Imunisasi Intranasal Epitope Protein RrgB 255-270 Streptococcus pneumoniae Terhadap Kadar IL-4 Widiatmaja, Danang Tejamukti; Mufida, Diana Chusna; Febianti, Zahrah
Sriwijaya Journal of Medicine Vol. 4 No. 1 (2021): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/SJM.v4i1.155

Abstract

Streptococcus pneumoniae or pneumococcus is the main cause of community acquired pneumoniae (CAP). Infection of pneumococcal can be prevented by vaccines, like PPV and PCV. Those vaccines have several weaknesses, like, limited to several strain of bacteria and invasive. Nowadays, development of pneumococcal vaccines used the antigenic component of which occur on the bacteria, like epitope. One of the epitopes that can be used as vaccine candidate is epitope from RrgB protein of bacteria’s pili. One of the RrgB protein’s epitope that has been identified having antigenic property is epitope protein RrgB 255-270 of Streptococcus pneumoniae bacteria’s pili. That epitope will stimulate immune system to produce antibody against the antigen supported by cytokine, like IL-4. This research aim is to knowing the effect of intranasal immunization epitope protein RrgB 255-270 Streptococcus pneumoniae to IL-4 level. Wistar rat’s nasal rinse sample IL-4 levels measured by ELISA method. The mean of K1 is 28,852± 18 ng/L, K2 is 20.630 ± 9 ng/L and K3 is 18.519 ± 6 ng/L. Result of Welch ANOVA test shows that p-value of this research is 0.299. That can be concluded that the effect of intranasal immunization epitope protein RrgB 255-270 S. pneumoniae give non-significant difference (p-value > 0.05).
Environmental Sanitation, Personal Hygiene, STH Co-infection in TB Patients Fitri, Nadya Eka; Mufida, Diana Chusna; Hermansyah, Bagus; Armiyanti, Yunita; Agustina, Dini; Suswanti, Enny
KEMAS: Jurnal Kesehatan Masyarakat Vol 16, No 3 (2021)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v16i3.23639

Abstract

Globally, helminthiasis is one of the infectious diseases that are often associated with the incidence of tuberculosis. Helminth co-infection modulates the immune system of TB patients by reducing Th-1 response that functions as protector against Mycobacterium tuberculosis. This condition disrupts the process of eliminating bacteria so that its development becomes more progressive. The impact is the activation of latent TB and the success of TB treatment. A Soil-transmitted Helminth (STH) is a group of intestinal worms that often infect humans. Previous studies prove that environmental sanitation and personal hygiene are risk factors associated with STH infection. The study to determine the relationship of environmental sanitation and personal hygiene with the incidence of STH co-infection in TB patients at Puskesmas Puger, Jember in September until December 2019. This study uses a cross-sectional design. The sampling technique uses total sampling. A total of 32 TB patients met the criteria as study sample. The data analysis uses Fisher Test. Results showed the prevalence of STH co-infection was relatively low (18.8%); most of the respondents had good environmental sanitation and personal hygiene conditions. Bivariate analysis showed p-value (0,476) for environmental sanitation and p-value (1,000) for personal hygiene. This study concludes that there is no correlation between environmental sanitation, personal hygiene, and STH co-infection in TB patients at Public health center in Puger.
Perubahan Morfologi Staphylococcus aureus Akibat Paparan Ekstrak Etanol Biji Kakao (Theobroma cacao) secara In Vitro Dafista Diyantika; Diana Chusna Mufida; Misnawi Misnawi
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Staphylococcus aureus (S. aureus) adalah bakteri Gram positif yang sering menginfeksi manusia. S. aureus resisten terhadap banyak antibiotik misalnya kloramfenikol, ampisilin, eritromisin, penisilin, dll. Ekstrak etanol biji kakao memiliki daya hambat terhadap pertumbuhan S. aureus secara in vitro, namun belum dapat ditunjukkan perubahan morfologi yang terjadi pada S. aureus setelah diberikan ekstrak etanol biji kakao. Tujuan dari penelitian ini adalah untuk mengkaji perubahan morfologi S. aureus setelah diberikan ekstrak etanol biji kakao. Jenis penelitian yang digunakan adalah Quasi Experimental dengan rancangan Posttest OnlyControl Group Design. Sampel yang digunakan adalah S. aureus dengan bahan uji yaitu ekstrak etanol biji kakao konsentrasi 31,2 mg/ml; 15,6 mg/ml; dan 7,8 mg/ml sedangkan kontrol negatifnya adalah aquades steril dan kontrol positifnya adalah suspensi sefaleksin 4 µg/ml. Metode yang digunakan untuk pengamatan perubahan morfologi S. aureus adalah uji scanning electron microscope. Hasil penelitian dapat disimpulkan bahwa ekstrak etanol biji kakao pada konsentrasi 15,6 mg/ml dan 7,8 mg/ml menyebabkan terjadinya perubahan morfologi S. aureus berupa pembesaran ukuran diameter sel dan tidak terpisahnya septum. Pada konsentrasi 31,25 mg/ml juga tampak adanya penonjolan pada dinding sel bakteri. Kata Kunci: Antimikroba, Kakao, SEM, Staphylococcus aureus
Interleukin-22-induced β‑defensin-2 expression by intranasal immunization with Streptococcus pneumoniae RrgB epitopes Mufida, Diana Chusna; Saputra, Antonius Dwi; Hermansyah, Bagus; Agustina, Dini; Shodikin, Muhammad Ali; Armiyanti, Yunita
Universa Medicina Vol. 41 No. 1 (2022)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2022.v41.37-46

Abstract

BackgroundStreptococcus pneumoniae causes pneumococcal disease, which is responsible for millions of deaths worldwide. Various pneumococcal vaccine candidates have been developed to prevent S. pneumoniae infection, one of which is an epitope-based vaccine. This study aimed to prove that intranasal immunization with each of the five S. pneumoniae RrgB epitopes can induce a mucosal immune response by increasing the β-defensin-2 concentration through upregulation of interleukin (IL)-22 expression. MethodsAn experimental laboratory study was conducted using 28 male Wistar rats aged 3-4 months, that were randomly divided into 7 groups containing four rats each. Group 1 was given 40 mL of phosphate-buffered saline (PBS) only (control group). Group 2 was the adjuvant group that received 40 mL PBS containing 2 ìg cholera toxin B (CTB), and groups 3-7 were immunized with 40 mL PBS containing a combination of adjuvant and one of the five different S. pneumoniae RrgB epitopes. The concentrations of IL-22 and β-defensin-2 from nasal rinse examination were measured by means of ELISA. The Kruskal-Wallis test, followed by the Mann-Whitney post-hoc test were used for statistical analysis. ResultsRats immunized with the adjuvant-epitope combination had significantly higher β-defensin-2 and IL-22 levels than the control group (p=0.030; p=0.018, respectively), according to the Kruskal-Wallis test. And the Mann-Whitney statistical test, showed there was a significant increase in β-defensin-2 and IL-22 levels. ConclusionsIntranasal immunization with epitope 1 of the S. pneumoniae RrgB can increase β-defensin-2 expression significantly and has a greater potential to be developed into a pneumococcal vaccine.