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All Journal Jurnal Natur Indonesia Jurnal Kesehatan Andalas Jurnal Farmasi Andalas Jurnal Sains dan Teknologi Farmasi Indonesian Journal of Pharmaceutical Science and Technology JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Kesehatan Medika Saintika JSFK (Jurnal Sains Farmasi & Klinis) Jurnal Farmasi Higea Human Care Journal Socius (Jurnal Pendidikan dan Pembelajaran Ilmu Pengetahuan Sosial) (Edisi Elektronik) Elevate : The International Journal of Nursing Education, Practice and Research
Netty Suharti
Fakultas Farmasi Universitas Andalas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Isolasi Jamur Pengurai Pati Dari Tanah Limbah Sagu Juwita, Dian Ayu; Suharti, Netty; Rasyid, Roslinda
Jurnal Farmasi Andalas Vol 1, No 1 (2013)
Publisher : Jurnal Farmasi Andalas

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Abstract

Screening of glucoamylase enzyme producing fungi obtained from sago dregs soil at Tapakis district of Padang Pariaman have been done. The screening was conducted by incubating of sample on Potato Dextrose Agar media previously added with starch 1% as an inducer. Incubation was performed during 120 hours at temperature of 20-250C. The existence of glucoamylase enzyme producing fungi were indicated with the disappearance of lugol indicator solution colour around the colonies. It was found that there were 2 isolates of fungi which produced glucoamylase. Following identification, it could be concluded that isolates were Aspergillus sp. and Penicillium sp.
Isolasi dan Uji Kualitatif Hidrolisat Jamur Penghasil Enzim Selulase dari Tanah Tumpukan Ampas Tebu Yosmar, Rahmi; Suharti, Netty; Rasyid, Roslinda
Jurnal Farmasi Andalas Vol 1, No 1 (2013)
Publisher : Jurnal Farmasi Andalas

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Abstract

A Study on the isolation and qualitative test  of cellulose producer fungus from waste product soil of Saccharum officinarum in Tabek Patah West Sumatera has been done by using  Czapek Dox Agar (CDA) selective media. The selulolitic ability test of this isolated fungus by giving the congo red solution on the surface of medium that was enriched with Carboxy Methyl Cellulose, the formed of transparent area around the colonies showed that the fungus has the capability to decomposed the cellulose. The hydrolitic enzyme in glucose form was analyed using Fehling solution and Thin Layer Chromatograph. The result from this research showed that the sample consist of two species of decomposed cellulose fungus Aspergillus terreus Thom and Rhizopus sp.
Karakterisasi Simplisia dan Ekstrak Etanol Serta Uji Aktivitas Antioksidan Rimpang Jahe Merah (Zingiber Officinale Var. Vubrum Theilade) yang Diinokulasi Fungi Mikoriza Arbuskula (FMA) Suharti, Netty; Lenggogeni, Yossi Gustria; Husni, Elidahanum
Jurnal Sains dan Teknologi Farmasi Vol 19 No Supl1 (2017): Vol 19 Supplement 1, December 2017
Publisher : Fakultas Farmasi Universitas Andalas

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Abstract

Jahe merah (Zingiber officinale var. rubrum Theilade) merupakan salah satu tanaman temu-temuan dari famili Zingiberaceae yang telah dimanfaatkan secara tradisional dalam pengobatan penyakit rematik, asma, stroke, sakit gigi, diabetes, sakit otot, tenggorokan, kram, hipertensi, mual, demam dan infeksi. Rimpag tanaman ini mengandung banyak senyawa kimia seperti gingerol, shogaol, zingiberen yang memiliki berbagai aktivitas farmakologi seperti antiinflamasi, antioksidan dan antikanker. Penelitian ini bertujuan untuk mengetahui karakterisasi simplisia dan ekstrak etanol rimpang jahe merah yang diinokulasi mikoriza, menentukan kadar fenolat total dengan metoda Folin-Ciocalteau dan uji aktivitas antioksidan dengan metode Ferric Reducing Antioxidant Power (FRAP). Rimpang jahe merah diambil di desa Bari, Padang Pariaman, Sumatera Barat dan dimaserasi menggunakan pelarut etanol 70% hingga didapatkan ekstrak etanol rimpang jahe merah. Dari karakterisasi simplisia rimpang jahe merah didapatkan diketahui bahwa bentuknya irisan, warna kuning kecokelatan, rasa pedas dan bau yang khas. Parameter susut pengeringan simplisia diperoleh sebesar 6,01 % ± 0,22, kadar abu total 7,42 % ± 0,86, kadar abu tidak larut asam 0,94 % ± 0,26, kadar sari larut air 13,55 % ± 1,11, dan kadar sari larut etanol 5,31 % ± 0,25. Karakterisasi ekstrak etanol rimpang jahe merah secara organolpetis diketahui  bahwa bentuknya cairan kental, warna kuning kecokelatan, rasa pedas dan bau yang khas. Parameter kadar air ekstrak etanol rimpang jahe merah adalah 9,33 % ± 1,53, kadar abu total 14,79 % ± 0,86, dan kadar abu tidak larut asam 0,97 % ± 0,26. Hasil penentuan kadar fenolat total ekstrak etanol rimpang jahe merah adalah 26,73 g/100 g. Aktivitas antioksidan ekstrak rimpang jahe merah yaitu 1,65 mmol Fe(II)/100 g.
Pengaruh Pemberian Jus Wortel (Daucus Carota, Linn) Terhadap Kadar Glukosa Darah Mencit Putih Betina Saputra, Hendra; Dharma, Surya; Suharti, Netty
Jurnal Sains dan Teknologi Farmasi Vol 16 No 2 (2011)
Publisher : Fakultas Farmasi Universitas Andalas

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Abstract

A study of the effect of carrot juice (Daucus carota, Linn) on blood glucose levels in female white mice. This experiment used Advantage blood glucose meter (Roche) with enzymatic method. Length of treatment was for 21 days with a time of observation on day 7th, 14th and 21st. Carrot juice dose given was 0.52; 1.04 and 2.08 g/20 g BW. Results showed that the addition of carrot juice at all dose lower the blood glucose levels in mice significantly (P <0.05) after giving alloxan 3 mg/20 g body weight and glucose 195 mg/20 g BW.
Isolation and Activity Assay of Secondary Metabolites of Aspergillus niger in-Habiting in Termite’s Queen Nest Macrotermes gilvus Hagen., on Enriched Media Alen, Yohannes; Guslianti, Evi; Suharti, Netty
Indonesian Journal of Pharmaceutical Science and Technology Vol 6, No 1 (2019 In Press)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.342 KB) | DOI: 10.15416/ijpst.v5i3.15735

Abstract

The Fungus is a group of the microorganisms that produce secondary metabolites. Secondary metabolites of fungus may be changed in different media. Secondary metabolites from Aspergillus niger in habiting in termite’s queen nest Macrotermes gilvus Hagen are disappeared gradually in artificial media. It was the reason to enrich the media with termite’s queen nest. The purpose of this research was to obtain the similar secondary metabolites of Aspergillus niger as it grows in their habitat. Enrichment was done with the experimental method. It used three concentrations of nest 0.25; 0.75 and 1 g/mL Sabouraud Dextrose Agar (SDA) media. Isolation was done use chromatography method. The antibiotic activities against Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 10541 were performed by the diffusion method. Results showed that enrichment of nest 1 g/mL media gave the best growth of fungus and it obtain the similar secondary metabolites as it grows in their habitat. Three pure compounds, EG-13-31-2, EG-13-34-9, and EG-13-44-2 were obtained. Based on physicochemical data, all compounds were terpenoid class and one of them (EG-13-34-9) contain the phenolic group. All compounds have activity against Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 10541 the bacteriostatic category.Key words: Aspergillus niger; Macrotermes gilvus Hagen; Sabouraud Dextrose Agar (SDA); Isolation; Enrich Media; Diffusion Method
Inokulasi Fungi Mikoriza Arbuskula (FMA) Indigenus pada Bibit Jahe untuk Pengendalian Penyakit Layu Ralstonia solanacearum ras 4) Suharti, Netty; Habazar, Trimurti; Nasir, Nasril; Dachryanus, Dachryanus; Jamsari, Jamsari
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.568 KB) | DOI: 10.31258/jnat.14.1.61-67

Abstract

Bacterial wilt disease caused by Ralstonia solanacearum race 4 is one of factors inhibiting ginger production. The study on ArbuscularMychorrizae Fungus (AMF) in greenhouse was found could reduce of bacteria wilt disease. Experiment design of research was randomizedblock design using 8 isolates. The results showed that inoculation of Arbuscular Mychorrhizae Fungus isolated from healthy gingerrhizosphere as biological diseases control agents could reduces disease severity. Four isolate could reduced disease severity up to 100% andsupport plant growth and production. As the result the seedling formation increased by 50–150%, plant height 36.92–87.56%, the numberof leaf 61.94–162.22% and ginger yield 190.62–400% respectively, compared to the control.
Identifikasi Pertumbuhan Jamur Aspergillus Sp pada Roti Tawar yang Dijual di Kota Padang Berdasarkan Suhu dan Lama Penyimpanan Dina Khaira Mizana; Netty Suharti; Arni Amir
Jurnal Kesehatan Andalas Vol 5, No 2 (2016)
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v5i2.521

Abstract

AbstrakPangan yang cukup banyak dikonsumsi masyarakat sebagai makanan kudapan di Indonesia adalah roti tawar. Jamur merupakan mikroorganisme utama yang berperan penting dalam proses pembuatan dan pembusukan roti. Aspergillus merupakan kontaminan umum pada berbagai substrat di daerah tropis maupun subtropik. Tujuan penelitian ini adalah membandingkan pertumbuhan jamur Aspergillus sp yang disimpan pada suhu kamar dan suhu di kulkas. Penelitian ini merupakan penelitian eksperimental analitik. Hasil penelitian menunjukan bahwa pertumbuhan jamur pada suhu kamar lebih cepat dibandingkan suhu kulkas. Pada suhu kamar 25C-28C, jamur Aspergillus sp tumbuh mulai hari ke-3 (33,3%) diikuti pada hari ke-4 (66,7%), sedangkan pada suhu kulkas (10C-15C) mulai tumbuh pada hari ke-5. Temperatur  ini juga berhubungan dengan kelembaban relatif karena semakin tinggi suhu maka  kelembaban relatif semakin rendah dan sebaliknya, semakin rendah suhu maka kelembaban relatif  akan semakin tinggi. Kesimpulan studi ini ialah terdapat perbedaaan pertumbuhan jamur Aspergillus sp yang disimpan pada suhu kamar dan suhu di kulkas walaupun perbedaannya tidak terlalu besar.Kata kunci: roti tawar, aspergillus sp, suhu, lama penyimpanan AbstractThe popular foods consumed by Indonesian citizens is loaves. Fungus is the main microorganism which has the main role in the process of loaves production and decomposition. Aspergillusis the eukaryotes microorganism that has the widest spread in the nature. This worm is also spreading in various substrates in the tropical and sub-tropical areas. The objective of this study was to compare the growth of Aspergillus sp stored in the room temperature and refrigerator temperature This research is analytic experimental. The results of this research showed  that the growth of fungus in the room temperature is faster than refrigerator temperature. In the room temperature, the fungus growth starts in the day-3 (33.3%) that is followed by dy-4 (66.7%), while the refrigerator temperature (100C-150C) shows that the fungus growth starts on day-5. The temperature is also related to the Relative Humidity (RH), the higher the temperature, so RH will become lower and vice versa. The foods which store in the low RH will be damaged on the surface side since the fungus, yeast, and certain bacteria. Hence, the fungus on loaves stored in the room temperature will be faster than loaves stored in the refrigerator temperature. There is a difference on the growth of Aspergillus sp stored in the room temperature and refrigerator temperature, even if the difference is not too big.Keywords: fresh bread,  aspergillus sp, temperature, prolonged storage
Kualitas Air Minum Yang Diproduksi Depot Air Minum Isi Ulang Di Kecamatan Bungus Padang Berdasarkan Persyaratan Mikrobiologi Rido Wandrivel; Netty Suharti; Yuniar Lestari
Jurnal Kesehatan Andalas Vol 1, No 3 (2012)
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v1i3.84

Abstract

Abstrak Latar belakang: Pendahuluan: Setiap depot air minum wajib melakukan pemeriksaan mutu produk sesuai dengan peraturan yang berlaku, namun tidak satupun dari sembilan depot air minum di Kecamatan Bungus melakukan hal tersebut. Diare adalah salah satu penyakit yang disebabkan oleh air minum yang tidak berkualitas. Morbiditas rate diare di Kecamatan Bungus tertinggi ke-2 di Kota Padang. Penelitian ini bertujuan untuk mengetahui kualitas air minum yang dihasilkan depot air minum di Kecamatan Bungus berdasarkan persyaratan mikrobiologi. Metode Penelitian: Populasi pada penelitian ini adalah air minum yang berasal dari depot air minum isi ulang di Kecamatan Bungus Padang dan sampel pada penelitian ini adalah seluruh populasi yang ada, yaitu sembilan sampel. Penelitian dilaksanakan dalam 2 tahap yaitu pengambilan sampel air dengan galon air sekaligus observasi faktor yang mempengaruhi kualitas air dan pemeriksaan mikrobiologis dengan Most Probable Number Test (MPN) terhadap sampel yang terdiri dari tiga tes, yaitu presumptive test, confirmative test, dan complete test. Hasil Penelitian: Lima dari sembilan sampel mengandung bakteri Coliform dan tiga dari lima sampel tersebut juga mengandung E. coli. Kesimpulan: Hal ini menunjukkan bahwa 55,6% depot air minum di Kecamatan Bungus menghasilkan air minum yang kualitasnya tidak memenuhi persyaratan mikrobiologi yang telah ditetapkan pemerintah. Beberapa faktor yang dapat mempengaruhi adalah air baku, kondisi depot, kebersihan operator, dan penanganan terhadap wadah pembeli. Kata kunci: Depot air minum isi ulang, pemeriksaan mikrobiologis Abstract Background: Every depot is obliged to do inspection of product quality prescribed by the regulations, but not one even also from nine drinking water depot in District of Bungus do the mentioned. Diarrhea is one of the disease that cause by bad quality water product. Diarrhea morbidity rate in the Bungus District is second highest in Padang City. This Research aim to to know the drinking water quality produced by drinking water refill depot in the Bungus District based on microbiological requirements. Methods: Population of this research is drinking water refill depots in the Bungus District of Padang city and sample of this research is all of existing population, that is nine drinking water depot. Research executed in 2 phase that is intake of water sample with gallon at the same time do observation of the factor that influencing the quality of water and mikrobiological test with Most Probable Number Test ( MPN) to the sampel which consist of three test, that is presumtive test, konfirmative test, and complete test. Result: Five from nine sample contain coliform bacteria and three from five the sampel also contain E. coli. Conclusion: This matter indicate that 55,6% drinking water refill depot in District of Bungus produce drinking water which its quality don’t fulfill microbiological requirements which have been specified by government. Some factor able to influence is standard water, condition of depot, hygiene of operator, and handling to place of buyer. Keywords: Drinking water refill depots, mikrobiological test
Isolation and Activity Assay of Secondary Metabolites of Aspergillus niger in-Habiting in Termite’s Queen Nest Macrotermes gilvus Hagen., on Enriched Media Yohannes Alen; Evi Guslianti; Netty Suharti
Indonesian Journal of Pharmaceutical Science and Technology Vol 6, No 1 (2019)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.342 KB) | DOI: 10.24198/ijpst.v6i1.15735

Abstract

The Fungus is a group of the microorganisms that produce secondary metabolites. Secondary metabolites of fungus may be changed in different media. Secondary metabolites from Aspergillus niger in habiting in termite’s queen nest Macrotermes gilvus Hagen are disappeared gradually in artificial media. It was the reason to enrich the media with termite’s queen nest. The purpose of this research was to obtain the similar secondary metabolites of Aspergillus niger as it grows in their habitat. Enrichment was done with the experimental method. It used three concentrations of nest 0.25; 0.75 and 1 g/mL Sabouraud Dextrose Agar (SDA) media. Isolation was done use chromatography method. The antibiotic activities against Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 10541 were performed by the diffusion method. Results showed that enrichment of nest 1 g/mL media gave the best growth of fungus and it obtain the similar secondary metabolites as it grows in their habitat. Three pure compounds, EG-13-31-2, EG-13-34-9, and EG-13-44-2 were obtained. Based on physicochemical data, all compounds were terpenoid class and one of them (EG-13-34-9) contain the phenolic group. All compounds have activity against Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 10541 the bacteriostatic category.Key words: Aspergillus niger; Macrotermes gilvus Hagen; Sabouraud Dextrose Agar (SDA); Isolation; Enrich Media; Diffusion Method
INDUKSI KETAHANAN TANAMAN JAHE TERHADAP PENYAKIT LAYU Ralstonia solanacearum RAS 4 MENGGUNAKAN FUNGI MIKORIZA ARBUSKULAR (FMA) INDIGENUS Netty Suharti; Trimurti Habazar; Nasril Nasir; . Dachryanus; . Jamsari
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 1 (2011): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.073 KB) | DOI: 10.23960/j.hptt.111102-111

Abstract

Bacterial wilt disease caused by Ralstonia solanacearum race 4 is a yield-limiting disease of ginger. A study to investigate the ability of indigenous Arbuscular Mycorrhizae Fungi (AMF) to reduce bacteria wilt disease has been done. This research was designed by using Randomized Block Design (RBD) at greenhouse experiments, using eight AMF isolates with six replication. As supporting data, population R. solanacearum race 4 after treatments was assesed. The results showed that aplication of indigenous AMF to ginger rhizome as seed treatment dicreased disease incidence, support ginger growth and production such as the seedling formation 150%, plant height 98.6%, the number of leaf 115,7%. As the result yield of ginger rhizome increased by 400 % and phyloplan 598,3%, respectively, compared to the control.
Co-Authors . Dachryanus Akmal Djamaan Arlyn Pasella Tri Atma Arni Amir Dachryanus, Dachryanus Dian Ayu Juwita Dina Khaira Mizana Eka Santi Adelinda Evi Guslianti Fajrian Aulia Putra Guslianti, Evi Harmawati Harmawati Helmi Arifin Hendra Saputra Hendra Saputra Henny Lucida Jamsari Jamsari Junuarty Jubahar Lenggogeni, Yossi Gustria Maria Ulfa Mira Susanti Nasril Nasir Nasril Nasir Olivia Sedona Rahmi Yosmar Rido Wandrivel Riris Friandi Rizki Febrika Putri Roslinda Rasyid Surya Dharma Surya Dharma TRIMURTI HABAZAR Yohannes Alen Yuanita Ananda Yuliana Astuti Yuniar Lestari Zulhidayati Zulhidayati